Elevated Level of Interleukin-29: A Proinflammatory Role in Synovial Inflammation of Rheumatoid Arthritis

Dalia A. Shaheen; Hisham M. Habib

doi:doi:10.11648/j.ab.20140202.11

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Elevated Level of Interleukin-29: A Proinflammatory Role in Synovial Inflammation of Rheumatoid Arthritis

Dalia A. Shaheen, Hisham M. Habib

Published in Advances in Biochemistry (Volume 2, Issue 2)

Received: 13 March 2014 Accepted: 9 April 2014 Published: 20 April 2014

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Abstract

Background: The multiple pro-inflammatory cascades described in RA lead to persistent synovitis, resulting in articular cartilage and bone damages. The up-regulation of interferon-inducible genes has been found in the synovial lining regions and may play an important role in the pathogenesis of RA. Objective: The aim of the current study is to examine the expression of IL-29 and its receptors IL-28Rα in blood and synovial fluid (SF) in RA patients and investigate the correlation of IL-29 with disease activity and other laboratory characters of RA. Methods: The study was carried out on 108 RA patients, 70 OA patients and 80 healthy control subjects. Clinical assessment of disease activity in RA patients and disease activity score with DAS28 had been done. IL29 was detected in the serum and synovial fluid of all samples by ELISA. Real time PCR was used for human IL-29 and IL-28Rα expression from PBMC. Results: expression of IL-29 mRNA was significantly higher in RA PBMCs when compared to OA and HC (132±13.8, 49± 11.2, 33.3± 10.5; respectively P <0.05). Similarly, IL-28Rα mRNA expression was significantly higher in RA PBMCs when compared to OA and HC (30.5±9.7, 13.3±4.5, 8.5± 3.3; respectively P <0.05). The mean level of IL-29 in SF was increased in RA and found also much higher in RA (17.29 ± 10.19pg/ml) than OA (8.33 ±3.89 pg/ml) (P = 0.01). There is a significant correlation between serum levels of circulating IL- 29 and DAS28, CRP, ESR, RF, and anti-CCP. Conclusion: our data have presented new evidence that IL-29 may contribute to RA pathogenesis

Published in	Advances in Biochemistry (Volume 2, Issue 2)
DOI	10.11648/j.ab.20140202.11
Page(s)	29-33
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2014. Published by Science Publishing Group

Keywords

IL29, Rheumatoid Arthritis, Synovial Inflammation

References

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APA Style

Dalia A. Shaheen, Hisham M. Habib. (2014). Elevated Level of Interleukin-29: A Proinflammatory Role in Synovial Inflammation of Rheumatoid Arthritis. Advances in Biochemistry, 2(2), 29-33. https://doi.org/10.11648/j.ab.20140202.11

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ACS Style

Dalia A. Shaheen; Hisham M. Habib. Elevated Level of Interleukin-29: A Proinflammatory Role in Synovial Inflammation of Rheumatoid Arthritis. Adv. Biochem. 2014, 2(2), 29-33. doi: 10.11648/j.ab.20140202.11

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AMA Style

Dalia A. Shaheen, Hisham M. Habib. Elevated Level of Interleukin-29: A Proinflammatory Role in Synovial Inflammation of Rheumatoid Arthritis. Adv Biochem. 2014;2(2):29-33. doi: 10.11648/j.ab.20140202.11

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@article{10.11648/j.ab.20140202.11,
  author = {Dalia A. Shaheen and Hisham M. Habib},
  title = {Elevated Level of Interleukin-29: A Proinflammatory Role in Synovial Inflammation of Rheumatoid Arthritis},
  journal = {Advances in Biochemistry},
  volume = {2},
  number = {2},
  pages = {29-33},
  doi = {10.11648/j.ab.20140202.11},
  url = {https://doi.org/10.11648/j.ab.20140202.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ab.20140202.11},
  abstract = {Background: The multiple pro-inflammatory cascades described in RA lead to persistent synovitis, resulting in articular cartilage and bone damages. The up-regulation of interferon-inducible genes has been found in the synovial lining regions and may play an important role in the pathogenesis of RA. Objective: The aim of the current study is to examine the expression of IL-29 and its receptors IL-28Rα in blood and synovial fluid (SF) in RA patients and investigate the correlation of IL-29 with disease activity and other laboratory characters of RA. Methods: The study was carried out on 108 RA patients, 70 OA patients and 80 healthy control subjects. Clinical assessment of disease activity in RA patients and disease activity score with DAS28 had been done. IL29 was detected in the serum and synovial fluid of all samples by ELISA. Real time PCR was used for human IL-29 and IL-28Rα expression from PBMC. Results: expression of IL-29 mRNA was significantly higher in RA PBMCs when compared to OA and HC (132±13.8, 49± 11.2, 33.3± 10.5; respectively P <0.05). Similarly, IL-28Rα mRNA expression was significantly higher in RA PBMCs when compared to OA and HC (30.5±9.7, 13.3±4.5, 8.5± 3.3; respectively P <0.05). The mean level of IL-29 in SF was increased in RA and found also much higher in RA (17.29 ± 10.19pg/ml) than OA (8.33 ±3.89 pg/ml) (P = 0.01). There is a significant correlation between serum levels of circulating IL- 29 and DAS28, CRP, ESR, RF, and anti-CCP. Conclusion: our data have presented new evidence that IL-29 may contribute to RA pathogenesis},
 year = {2014}
}

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TY  - JOUR
T1  - Elevated Level of Interleukin-29: A Proinflammatory Role in Synovial Inflammation of Rheumatoid Arthritis
AU  - Dalia A. Shaheen
AU  - Hisham M. Habib
Y1  - 2014/04/20
PY  - 2014
N1  - https://doi.org/10.11648/j.ab.20140202.11
DO  - 10.11648/j.ab.20140202.11
T2  - Advances in Biochemistry
JF  - Advances in Biochemistry
JO  - Advances in Biochemistry
SP  - 29
EP  - 33
PB  - Science Publishing Group
SN  - 2329-0862
UR  - https://doi.org/10.11648/j.ab.20140202.11
AB  - Background: The multiple pro-inflammatory cascades described in RA lead to persistent synovitis, resulting in articular cartilage and bone damages. The up-regulation of interferon-inducible genes has been found in the synovial lining regions and may play an important role in the pathogenesis of RA. Objective: The aim of the current study is to examine the expression of IL-29 and its receptors IL-28Rα in blood and synovial fluid (SF) in RA patients and investigate the correlation of IL-29 with disease activity and other laboratory characters of RA. Methods: The study was carried out on 108 RA patients, 70 OA patients and 80 healthy control subjects. Clinical assessment of disease activity in RA patients and disease activity score with DAS28 had been done. IL29 was detected in the serum and synovial fluid of all samples by ELISA. Real time PCR was used for human IL-29 and IL-28Rα expression from PBMC. Results: expression of IL-29 mRNA was significantly higher in RA PBMCs when compared to OA and HC (132±13.8, 49± 11.2, 33.3± 10.5; respectively P <0.05). Similarly, IL-28Rα mRNA expression was significantly higher in RA PBMCs when compared to OA and HC (30.5±9.7, 13.3±4.5, 8.5± 3.3; respectively P <0.05). The mean level of IL-29 in SF was increased in RA and found also much higher in RA (17.29 ± 10.19pg/ml) than OA (8.33 ±3.89 pg/ml) (P = 0.01). There is a significant correlation between serum levels of circulating IL- 29 and DAS28, CRP, ESR, RF, and anti-CCP. Conclusion: our data have presented new evidence that IL-29 may contribute to RA pathogenesis
VL  - 2
IS  - 2
ER  -

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Author Information

Dalia A. Shaheen

Department of Medical Biochemistry, Faculty of Medicine, Mansoura University, Egypt
Hisham M. Habib

Department of Rheumatology and Rehabilitation, Faculty of Medicine, Mansoura University, Egypt

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Dalia A. Shaheen, Hisham M. Habib. (2014). Elevated Level of Interleukin-29: A Proinflammatory Role in Synovial Inflammation of Rheumatoid Arthritis. Advances in Biochemistry, 2(2), 29-33. https://doi.org/10.11648/j.ab.20140202.11

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ACS Style

Dalia A. Shaheen; Hisham M. Habib. Elevated Level of Interleukin-29: A Proinflammatory Role in Synovial Inflammation of Rheumatoid Arthritis. Adv. Biochem. 2014, 2(2), 29-33. doi: 10.11648/j.ab.20140202.11

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AMA Style

Dalia A. Shaheen, Hisham M. Habib. Elevated Level of Interleukin-29: A Proinflammatory Role in Synovial Inflammation of Rheumatoid Arthritis. Adv Biochem. 2014;2(2):29-33. doi: 10.11648/j.ab.20140202.11

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@article{10.11648/j.ab.20140202.11,
  author = {Dalia A. Shaheen and Hisham M. Habib},
  title = {Elevated Level of Interleukin-29: A Proinflammatory Role in Synovial Inflammation of Rheumatoid Arthritis},
  journal = {Advances in Biochemistry},
  volume = {2},
  number = {2},
  pages = {29-33},
  doi = {10.11648/j.ab.20140202.11},
  url = {https://doi.org/10.11648/j.ab.20140202.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ab.20140202.11},
  abstract = {Background: The multiple pro-inflammatory cascades described in RA lead to persistent synovitis, resulting in articular cartilage and bone damages. The up-regulation of interferon-inducible genes has been found in the synovial lining regions and may play an important role in the pathogenesis of RA. Objective: The aim of the current study is to examine the expression of IL-29 and its receptors IL-28Rα in blood and synovial fluid (SF) in RA patients and investigate the correlation of IL-29 with disease activity and other laboratory characters of RA. Methods: The study was carried out on 108 RA patients, 70 OA patients and 80 healthy control subjects. Clinical assessment of disease activity in RA patients and disease activity score with DAS28 had been done. IL29 was detected in the serum and synovial fluid of all samples by ELISA. Real time PCR was used for human IL-29 and IL-28Rα expression from PBMC. Results: expression of IL-29 mRNA was significantly higher in RA PBMCs when compared to OA and HC (132±13.8, 49± 11.2, 33.3± 10.5; respectively P <0.05). Similarly, IL-28Rα mRNA expression was significantly higher in RA PBMCs when compared to OA and HC (30.5±9.7, 13.3±4.5, 8.5± 3.3; respectively P <0.05). The mean level of IL-29 in SF was increased in RA and found also much higher in RA (17.29 ± 10.19pg/ml) than OA (8.33 ±3.89 pg/ml) (P = 0.01). There is a significant correlation between serum levels of circulating IL- 29 and DAS28, CRP, ESR, RF, and anti-CCP. Conclusion: our data have presented new evidence that IL-29 may contribute to RA pathogenesis},
 year = {2014}
}

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TY  - JOUR
T1  - Elevated Level of Interleukin-29: A Proinflammatory Role in Synovial Inflammation of Rheumatoid Arthritis
AU  - Dalia A. Shaheen
AU  - Hisham M. Habib
Y1  - 2014/04/20
PY  - 2014
N1  - https://doi.org/10.11648/j.ab.20140202.11
DO  - 10.11648/j.ab.20140202.11
T2  - Advances in Biochemistry
JF  - Advances in Biochemistry
JO  - Advances in Biochemistry
SP  - 29
EP  - 33
PB  - Science Publishing Group
SN  - 2329-0862
UR  - https://doi.org/10.11648/j.ab.20140202.11
AB  - Background: The multiple pro-inflammatory cascades described in RA lead to persistent synovitis, resulting in articular cartilage and bone damages. The up-regulation of interferon-inducible genes has been found in the synovial lining regions and may play an important role in the pathogenesis of RA. Objective: The aim of the current study is to examine the expression of IL-29 and its receptors IL-28Rα in blood and synovial fluid (SF) in RA patients and investigate the correlation of IL-29 with disease activity and other laboratory characters of RA. Methods: The study was carried out on 108 RA patients, 70 OA patients and 80 healthy control subjects. Clinical assessment of disease activity in RA patients and disease activity score with DAS28 had been done. IL29 was detected in the serum and synovial fluid of all samples by ELISA. Real time PCR was used for human IL-29 and IL-28Rα expression from PBMC. Results: expression of IL-29 mRNA was significantly higher in RA PBMCs when compared to OA and HC (132±13.8, 49± 11.2, 33.3± 10.5; respectively P <0.05). Similarly, IL-28Rα mRNA expression was significantly higher in RA PBMCs when compared to OA and HC (30.5±9.7, 13.3±4.5, 8.5± 3.3; respectively P <0.05). The mean level of IL-29 in SF was increased in RA and found also much higher in RA (17.29 ± 10.19pg/ml) than OA (8.33 ±3.89 pg/ml) (P = 0.01). There is a significant correlation between serum levels of circulating IL- 29 and DAS28, CRP, ESR, RF, and anti-CCP. Conclusion: our data have presented new evidence that IL-29 may contribute to RA pathogenesis
VL  - 2
IS  - 2
ER  -

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