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Phytochemical Screening, Chemical Composition and Antioxidant Activity of Leaves and Bark Extracts from Khaya senegalensis

Received: 5 September 2018     Accepted: 30 September 2018     Published: 1 November 2018
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Abstract

Khaya senegalensis belonging to family Meliaceae. This plant has been used as traditional medicine for several disease such as pain, inflammation, malaria, anemia, diabetic and gastrointestinal diseases. The objective of this study to carry out phytochemical screening, determination of chemical composition and determine antioxidant activity of plant extracts. Many of secondary metabolites were investigated in leaves extract include alkaloids, flavonoids, sterols and saponins while the bark extract includes sterols, triterpines, tannins and saponins. The antioxidant potential of ethanol extract of leaves and bark was determined on the basis of their scavenging activity of the stable 1, 1-diphenyl-2-picryl hydrazyl (DPPH) free radical. Antioxidant activity of leaves extract was higher than bark with (42.69%) and (36.36%) respectively. The quantitative analysis of chemical composition was determined by Gas Chromatography–Mass Spectrometry (GC-MS). A number of bioactive compounds were present in the leaves and bark of K.senegalensis. The highest compound in both extracts represented the reducing sugar 4-O-Methyl mannose (66.47 and 14.73%) in bark and leaves respectively. Fatty acids and phenols were presented in high percentage in both extract include; n-Hexadecanoic acid, 9,12,15-Octadecatrienoic acid, Oleic Acid and Catechol.

Published in Advances in Biochemistry (Volume 6, Issue 4)
DOI 10.11648/j.ab.20180604.12
Page(s) 32-38
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2018. Published by Science Publishing Group

Keywords

Khaya senegalensis, Leaves, Bark, Phytochemical Screening, GC-MS Analysis, Antioxidant Activity

References
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[4] Elisha, I. L., Makoshi, M. S., Makama, S., Dawurung, C. J., Offiah, N. V., Gotep, J. G., Oladipo, O. and Shamaki, D. Antidiarrheal evaluation of aqueous and ethanolic stem bark extracts of Khaya senegalensis A. Juss (Meliaceae) in albino rats. Pak. Vet. J., 2013; 33(1): 32-36.
[5] Trease, G. E., Evans, W. C. Textbook of Pharmacognosy. 15th Ed.,2002. Saunders Publishers, London.
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[7] Alrasheid, A. A., Abdallah, B. S., Ali. A. O. (2018). In Vitro Antimicrobial Activity and GC-MS Analysis of Seed Extracts from Pimpinella anisum L. Journal of Drug Design and Medicinal Chemistry., 4( 2):16-21.
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[9] Braca A, Tommasi ND, Bari LD, Pizza C, Politi M, Morelli I (2001). Natural anti-oxidants from plant material in phenolic compounds in food and their effects on health. J. Nat. Prod., 64: 892-895.
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[11] Aguoru, C. U., Bashayi, C. G and Ogbonna, I. O (2017). Phytochemical profile of stem bark extracts of Khaya senegalensisby Gas Chromatography-Mass Spectrometry (GC-MS) analysis. Journal of Pharmacognosy and Phytotherapy;9(3):35-43.
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[14] Wakirwa JH, Idris S, Madu SJ, Dibal M, Malgwi T (2013). Assessment of the In-vitro antimicrobial potential of Khaya senegalensi sethanol leaf extract. J. Chem. Pharm. Res. 5(7):182-186.
[15] Kuta, F. A., Tsado, D. J., Garba, S. A and Saidu, A. N (2015). Antibacterial activity of the leaf and stem bark crude extracts of Khaya senegalensis. Med. and Aromatic Plant Res. J., 3(1): 9-15. 2015.
[16] Van de Venter M, Roux S, Bungu LC, et al. Antidiabetic screening and scoring of 11plants traditionally used in South Africa. JEthnopharmacol2008; 119: 81–86.
[17] Ibrahim. M. A., Koorbanally, N. A and Islam, M. S. Anti-oxidative activity and inhibition of key enzymes linked to type 2 diabetes (α-glucosidase and α-amylase) by Khaya senegalensis Records of Natural Products. 2013, 195-200.
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[19] Shittu LAJ, Bankole MA, Ahmed T, Bankole MN, Shittu RK, Saalu CL, Ashiru OA (2007). Antibacterial and antifungal activities of essential oils of crude extracts of Sesame radiatum against some common pathogenic microorganisms. Iran J. Pharmacol. Ther. 6:165-170.
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    Marvit Osman Widdat Allah, Ayat Ahmed Alrasheid, Altayeb Suliman Elamin. (2018). Phytochemical Screening, Chemical Composition and Antioxidant Activity of Leaves and Bark Extracts from Khaya senegalensis. Advances in Biochemistry, 6(4), 32-38. https://doi.org/10.11648/j.ab.20180604.12

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    ACS Style

    Marvit Osman Widdat Allah; Ayat Ahmed Alrasheid; Altayeb Suliman Elamin. Phytochemical Screening, Chemical Composition and Antioxidant Activity of Leaves and Bark Extracts from Khaya senegalensis. Adv. Biochem. 2018, 6(4), 32-38. doi: 10.11648/j.ab.20180604.12

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    AMA Style

    Marvit Osman Widdat Allah, Ayat Ahmed Alrasheid, Altayeb Suliman Elamin. Phytochemical Screening, Chemical Composition and Antioxidant Activity of Leaves and Bark Extracts from Khaya senegalensis. Adv Biochem. 2018;6(4):32-38. doi: 10.11648/j.ab.20180604.12

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  • @article{10.11648/j.ab.20180604.12,
      author = {Marvit Osman Widdat Allah and Ayat Ahmed Alrasheid and Altayeb Suliman Elamin},
      title = {Phytochemical Screening, Chemical Composition and Antioxidant Activity of Leaves and Bark Extracts from Khaya senegalensis},
      journal = {Advances in Biochemistry},
      volume = {6},
      number = {4},
      pages = {32-38},
      doi = {10.11648/j.ab.20180604.12},
      url = {https://doi.org/10.11648/j.ab.20180604.12},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ab.20180604.12},
      abstract = {Khaya senegalensis belonging to family Meliaceae. This plant has been used as traditional medicine for several disease such as pain, inflammation, malaria, anemia, diabetic and gastrointestinal diseases. The objective of this study to carry out phytochemical screening, determination of chemical composition and determine antioxidant activity of plant extracts. Many of secondary metabolites were investigated in leaves extract include alkaloids, flavonoids, sterols and saponins while the bark extract includes sterols, triterpines, tannins and saponins. The antioxidant potential of ethanol extract of leaves and bark was determined on the basis of their scavenging activity of the stable 1, 1-diphenyl-2-picryl hydrazyl (DPPH) free radical. Antioxidant activity of leaves extract was higher than bark with (42.69%) and (36.36%) respectively. The quantitative analysis of chemical composition was determined by Gas Chromatography–Mass Spectrometry (GC-MS). A number of bioactive compounds were present in the leaves and bark of K.senegalensis. The highest compound in both extracts represented the reducing sugar 4-O-Methyl mannose (66.47 and 14.73%) in bark and leaves respectively. Fatty acids and phenols were presented in high percentage in both extract include; n-Hexadecanoic acid, 9,12,15-Octadecatrienoic acid, Oleic Acid and Catechol.},
     year = {2018}
    }
    

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  • TY  - JOUR
    T1  - Phytochemical Screening, Chemical Composition and Antioxidant Activity of Leaves and Bark Extracts from Khaya senegalensis
    AU  - Marvit Osman Widdat Allah
    AU  - Ayat Ahmed Alrasheid
    AU  - Altayeb Suliman Elamin
    Y1  - 2018/11/01
    PY  - 2018
    N1  - https://doi.org/10.11648/j.ab.20180604.12
    DO  - 10.11648/j.ab.20180604.12
    T2  - Advances in Biochemistry
    JF  - Advances in Biochemistry
    JO  - Advances in Biochemistry
    SP  - 32
    EP  - 38
    PB  - Science Publishing Group
    SN  - 2329-0862
    UR  - https://doi.org/10.11648/j.ab.20180604.12
    AB  - Khaya senegalensis belonging to family Meliaceae. This plant has been used as traditional medicine for several disease such as pain, inflammation, malaria, anemia, diabetic and gastrointestinal diseases. The objective of this study to carry out phytochemical screening, determination of chemical composition and determine antioxidant activity of plant extracts. Many of secondary metabolites were investigated in leaves extract include alkaloids, flavonoids, sterols and saponins while the bark extract includes sterols, triterpines, tannins and saponins. The antioxidant potential of ethanol extract of leaves and bark was determined on the basis of their scavenging activity of the stable 1, 1-diphenyl-2-picryl hydrazyl (DPPH) free radical. Antioxidant activity of leaves extract was higher than bark with (42.69%) and (36.36%) respectively. The quantitative analysis of chemical composition was determined by Gas Chromatography–Mass Spectrometry (GC-MS). A number of bioactive compounds were present in the leaves and bark of K.senegalensis. The highest compound in both extracts represented the reducing sugar 4-O-Methyl mannose (66.47 and 14.73%) in bark and leaves respectively. Fatty acids and phenols were presented in high percentage in both extract include; n-Hexadecanoic acid, 9,12,15-Octadecatrienoic acid, Oleic Acid and Catechol.
    VL  - 6
    IS  - 4
    ER  - 

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Author Information
  • Department of Pharmacology, Faculty of Pharmacy, University of Medical Sciences and Technology, Khartoum, Sudan

  • Department of Pharmacognosy, Faculty of Pharmacy, University of Medical Sciences and Technology, Khartoum, Sudan

  • Department of Pharmaceutics, Faculty of Pharmacy, Omdurman Islamic University, Khartoum, Sudan

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