Studies on Qualitative and Quantitative Estimation of Primary and Secondary Metabolites in Various Solvents Extracts of <i>Aegle marmelos</i>

Hanumantappa Bherigi Nayaka; Ramesh Londonkar

doi:doi:10.11648/j.ab.20190701.12

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Studies on Qualitative and Quantitative Estimation of Primary and Secondary Metabolites in Various Solvents Extracts of Aegle marmelos

Hanumantappa Bherigi Nayaka, Ramesh Londonkar

Published in Advances in Biochemistry (Volume 7, Issue 1)

Received: 10 April 2019 Accepted: 21 May 2019 Published: 10 June 2019

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Abstract

The present study was an attempt to analyze the qualitative and quantitative analysis of phytochemical in different solvents extracts of A. marmelos. Primary metabolites like total soluble carbohydrates, proteins and secondary metabolites such as flavonoids, total phenols, and tannins were estimated using standard protocols. The qualitative and quantitative analysis is very essential for identifying and quantifying the compounds present in the medicinal plants. The results obtained from the present study provides evidence that various solvent extracts of A. marmelos leaves contains different types of primary and secondary metabolites which are having highly medicinal values and this justifies that the use of plant species as traditional medicine for treatment of various diseases. The finding of this study suggests that this plant leaves could be a potential source of natural medicine that could have great importance as therapeutic agents in preventing various diseases. The results are very much encouraging but scientific validation is necessary before being put in to practice. The present study suggest for future researcher on this plant that further Isolation, purification, characterization, structural elucidation and evaluation of a specific biological activity and preclinical and clinical studies of Bioactive compounds of this plant extracts were also necessary to emerge as medicine in the market.

Published in	Advances in Biochemistry (Volume 7, Issue 1)
DOI	10.11648/j.ab.20190701.12
Page(s)	5-9
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2019. Published by Science Publishing Group

Keywords

Aegle marmelos, Leaves, Secondary Metabolites, Phytochemical Screening, Medicinal Plant

References

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[3]	Oluma H O, Umoh E U, Onekutu A and Okolo J. Antibacterial potentials of eight medicinal plants from the lower Benue valley of Nigeria against Salmonella typhi., Journal of Botany. 2004: 17: 1-11.
[4]	Shabir G. S, Bahadur and Choudhry M. R, Botanical Description, Significance and production Technology of Some important medicinal herbs. Hamdard medicus, 2003. 1, 23-26.
[5]	Harbone J B, Phytochemical Methods, pp. 49–188, Chapman and Hall, London, UK, 1973.
[6]	Sofowara, A. Medicinal plants and traditional medicine in Africa. Spectrum Books Ltd., Ibadan, Nigeria, 1993.
[7]	Hedge, J. E. and Hofreiter, B. T. Carbohydrate chemistry 17. Whistler, R. L. and Be Miller, J. N., Eds., Academic Press, New York, 1962.
[8]	Stanford Moore and; William H. Stein. photometric Ninhydrin method for use in the chromatography of amino acids J. Biol. Chem. 1948.
[9]	Lowry, O. H. N., Rosenbrough. J. Farr, N. L and Randall. R. J., Protein measurement with folic phenol reagent J. Biol. Chem. 1951. 193, 265-275.
[10]	Zhu H, Wang Y, Liu Y, Xia Y, and Tang T, “Analysis of flavonoids in Portulaca oleracea L. by UV-vis spectrophotometry with comparative study on different extraction technologies, “Food Analytical Methods, 2010. vol. 3, no. 2, pp. 90–97.
[11]	Manjush Ramesh. W Aparna K and Ramesh C. Phytochemical screening and antimicrobial evaluation of Cassia anriculata L, Bionano frontier, 2010. vol. 3, 2.
[12]	Baskaran C, Ratho bai V, Kanimozhi D. Screening for antibacterial activity and phytochemical analysis of various leaf extract of Murrya koenigi IJRAP, 2012. 2, 6, PP 1807-8110.
[13]	Chinnappan A. Antimicrobial activity and Phytochemical analysis of Andrographis alata Nees from Southern India. Int J of PharmTech Research, 2011. vol. 3, No. 3, pp 1322-1328.

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Hanumantappa Bherigi Nayaka, Ramesh Londonkar. (2019). Studies on Qualitative and Quantitative Estimation of Primary and Secondary Metabolites in Various Solvents Extracts of Aegle marmelos. Advances in Biochemistry, 7(1), 5-9. https://doi.org/10.11648/j.ab.20190701.12

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ACS Style

Hanumantappa Bherigi Nayaka; Ramesh Londonkar. Studies on Qualitative and Quantitative Estimation of Primary and Secondary Metabolites in Various Solvents Extracts of Aegle marmelos. Adv. Biochem. 2019, 7(1), 5-9. doi: 10.11648/j.ab.20190701.12

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AMA Style

Hanumantappa Bherigi Nayaka, Ramesh Londonkar. Studies on Qualitative and Quantitative Estimation of Primary and Secondary Metabolites in Various Solvents Extracts of Aegle marmelos. Adv Biochem. 2019;7(1):5-9. doi: 10.11648/j.ab.20190701.12

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@article{10.11648/j.ab.20190701.12,
  author = {Hanumantappa Bherigi Nayaka and Ramesh Londonkar},
  title = {Studies on Qualitative and Quantitative Estimation of Primary and Secondary Metabolites in Various Solvents Extracts of Aegle marmelos},
  journal = {Advances in Biochemistry},
  volume = {7},
  number = {1},
  pages = {5-9},
  doi = {10.11648/j.ab.20190701.12},
  url = {https://doi.org/10.11648/j.ab.20190701.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ab.20190701.12},
  abstract = {The present study was an attempt to analyze the qualitative and quantitative analysis of phytochemical in different solvents extracts of A. marmelos. Primary metabolites like total soluble carbohydrates, proteins and secondary metabolites such as flavonoids, total phenols, and tannins were estimated using standard protocols. The qualitative and quantitative analysis is very essential for identifying and quantifying the compounds present in the medicinal plants. The results obtained from the present study provides evidence that various solvent extracts of A. marmelos leaves contains different types of primary and secondary metabolites which are having highly medicinal values and this justifies that the use of plant species as traditional medicine for treatment of various diseases. The finding of this study suggests that this plant leaves could be a potential source of natural medicine that could have great importance as therapeutic agents in preventing various diseases. The results are very much encouraging but scientific validation is necessary before being put in to practice. The present study suggest for future researcher on this plant that further Isolation, purification, characterization, structural elucidation and evaluation of a specific biological activity and preclinical and clinical studies of Bioactive compounds of this plant extracts were also necessary to emerge as medicine in the market.},
 year = {2019}
}

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TY  - JOUR
T1  - Studies on Qualitative and Quantitative Estimation of Primary and Secondary Metabolites in Various Solvents Extracts of Aegle marmelos
AU  - Hanumantappa Bherigi Nayaka
AU  - Ramesh Londonkar
Y1  - 2019/06/10
PY  - 2019
N1  - https://doi.org/10.11648/j.ab.20190701.12
DO  - 10.11648/j.ab.20190701.12
T2  - Advances in Biochemistry
JF  - Advances in Biochemistry
JO  - Advances in Biochemistry
SP  - 5
EP  - 9
PB  - Science Publishing Group
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UR  - https://doi.org/10.11648/j.ab.20190701.12
AB  - The present study was an attempt to analyze the qualitative and quantitative analysis of phytochemical in different solvents extracts of A. marmelos. Primary metabolites like total soluble carbohydrates, proteins and secondary metabolites such as flavonoids, total phenols, and tannins were estimated using standard protocols. The qualitative and quantitative analysis is very essential for identifying and quantifying the compounds present in the medicinal plants. The results obtained from the present study provides evidence that various solvent extracts of A. marmelos leaves contains different types of primary and secondary metabolites which are having highly medicinal values and this justifies that the use of plant species as traditional medicine for treatment of various diseases. The finding of this study suggests that this plant leaves could be a potential source of natural medicine that could have great importance as therapeutic agents in preventing various diseases. The results are very much encouraging but scientific validation is necessary before being put in to practice. The present study suggest for future researcher on this plant that further Isolation, purification, characterization, structural elucidation and evaluation of a specific biological activity and preclinical and clinical studies of Bioactive compounds of this plant extracts were also necessary to emerge as medicine in the market.
VL  - 7
IS  - 1
ER  -

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Author Information

Hanumantappa Bherigi Nayaka

Department of Life Sciences, Faculty of Sciences, Kristu Jayanti College Autonomous, Bengaluru, India
Ramesh Londonkar

Department of Post Graduate Studies and Research in Biotechnology, Faculty of Science and Technology, Gulbarga University, Kalburgi, India

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APA Style

Hanumantappa Bherigi Nayaka, Ramesh Londonkar. (2019). Studies on Qualitative and Quantitative Estimation of Primary and Secondary Metabolites in Various Solvents Extracts of Aegle marmelos. Advances in Biochemistry, 7(1), 5-9. https://doi.org/10.11648/j.ab.20190701.12

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ACS Style

Hanumantappa Bherigi Nayaka; Ramesh Londonkar. Studies on Qualitative and Quantitative Estimation of Primary and Secondary Metabolites in Various Solvents Extracts of Aegle marmelos. Adv. Biochem. 2019, 7(1), 5-9. doi: 10.11648/j.ab.20190701.12

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AMA Style

Hanumantappa Bherigi Nayaka, Ramesh Londonkar. Studies on Qualitative and Quantitative Estimation of Primary and Secondary Metabolites in Various Solvents Extracts of Aegle marmelos. Adv Biochem. 2019;7(1):5-9. doi: 10.11648/j.ab.20190701.12

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@article{10.11648/j.ab.20190701.12,
  author = {Hanumantappa Bherigi Nayaka and Ramesh Londonkar},
  title = {Studies on Qualitative and Quantitative Estimation of Primary and Secondary Metabolites in Various Solvents Extracts of Aegle marmelos},
  journal = {Advances in Biochemistry},
  volume = {7},
  number = {1},
  pages = {5-9},
  doi = {10.11648/j.ab.20190701.12},
  url = {https://doi.org/10.11648/j.ab.20190701.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ab.20190701.12},
  abstract = {The present study was an attempt to analyze the qualitative and quantitative analysis of phytochemical in different solvents extracts of A. marmelos. Primary metabolites like total soluble carbohydrates, proteins and secondary metabolites such as flavonoids, total phenols, and tannins were estimated using standard protocols. The qualitative and quantitative analysis is very essential for identifying and quantifying the compounds present in the medicinal plants. The results obtained from the present study provides evidence that various solvent extracts of A. marmelos leaves contains different types of primary and secondary metabolites which are having highly medicinal values and this justifies that the use of plant species as traditional medicine for treatment of various diseases. The finding of this study suggests that this plant leaves could be a potential source of natural medicine that could have great importance as therapeutic agents in preventing various diseases. The results are very much encouraging but scientific validation is necessary before being put in to practice. The present study suggest for future researcher on this plant that further Isolation, purification, characterization, structural elucidation and evaluation of a specific biological activity and preclinical and clinical studies of Bioactive compounds of this plant extracts were also necessary to emerge as medicine in the market.},
 year = {2019}
}

Copy | Download

TY  - JOUR
T1  - Studies on Qualitative and Quantitative Estimation of Primary and Secondary Metabolites in Various Solvents Extracts of Aegle marmelos
AU  - Hanumantappa Bherigi Nayaka
AU  - Ramesh Londonkar
Y1  - 2019/06/10
PY  - 2019
N1  - https://doi.org/10.11648/j.ab.20190701.12
DO  - 10.11648/j.ab.20190701.12
T2  - Advances in Biochemistry
JF  - Advances in Biochemistry
JO  - Advances in Biochemistry
SP  - 5
EP  - 9
PB  - Science Publishing Group
SN  - 2329-0862
UR  - https://doi.org/10.11648/j.ab.20190701.12
AB  - The present study was an attempt to analyze the qualitative and quantitative analysis of phytochemical in different solvents extracts of A. marmelos. Primary metabolites like total soluble carbohydrates, proteins and secondary metabolites such as flavonoids, total phenols, and tannins were estimated using standard protocols. The qualitative and quantitative analysis is very essential for identifying and quantifying the compounds present in the medicinal plants. The results obtained from the present study provides evidence that various solvent extracts of A. marmelos leaves contains different types of primary and secondary metabolites which are having highly medicinal values and this justifies that the use of plant species as traditional medicine for treatment of various diseases. The finding of this study suggests that this plant leaves could be a potential source of natural medicine that could have great importance as therapeutic agents in preventing various diseases. The results are very much encouraging but scientific validation is necessary before being put in to practice. The present study suggest for future researcher on this plant that further Isolation, purification, characterization, structural elucidation and evaluation of a specific biological activity and preclinical and clinical studies of Bioactive compounds of this plant extracts were also necessary to emerge as medicine in the market.
VL  - 7
IS  - 1
ER  -

Copy | Download