Influence of Different Cowpea (<i>Vigna unguiculata</i> (L.) Walp.) Genotypes from Burkina Faso on Proteases Inhibition

Pierre Alexandre Eric Djifaby Sombié; Ahmed Yacouba Coulibaly; Adama Hilou; Martin Kiendrebéogo

doi:doi:10.11648/j.ab.20190701.14

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Influence of Different Cowpea (Vigna unguiculata (L.) Walp.) Genotypes from Burkina Faso on Proteases Inhibition

Pierre Alexandre Eric Djifaby Sombié, Ahmed Yacouba Coulibaly, Adama Hilou, Martin Kiendrebéogo

Published in Advances in Biochemistry (Volume 7, Issue 1)

Received: 6 April 2019 Accepted: 23 May 2019 Published: 11 June 2019

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Abstract

Cowpea is an important protein crop widespread in Africa. The purpose of this research was to determine the content of trypsin and chymotrypsin inhibitors in different genotypes of cowpea seeds. Trypsin percentage inhibition showed about 13.5-fold variation (5.12% ± 1.47 to 70.52% ± 6.58) between the cowpea genotypes respectively for KVx 421-2J and Kondèsyoungo local. The chymotrypsin inhibitory activity varies among cowpea genotypes from 21.19 to 76.94%. The highest percent inhibitory activity was exhibited by KVx 396-4-5-2D on chymotrypsin. This study also showed significant correlations between type of cowpea genotype and trypsin inhibition potential and also between colour and chymotrypsin inhibition potential of cowpea seeds. Kondèsyoungo local, a landraceae genotype exhibited the high potential to inhibit the trypsin enzyme. Coloured seeds of cowpea genotypes possess higher percentage inhibition of chymotrypsin than the colourless ones (p < 0.05). The calculated mean of trypsin and chymotrypsin inhibition activities showed that Labagela local genotype possess the highest inhibition activity of both protease enzymes. The PCA components analysis and the dendrogram performed basis on the protease inhibitory activities divided the thirty-one genotypes of cowpea used in this study into three classes. The results presented in this work can contribute greatly to the planning of a cowpea breeding program aimed at reducing the content of proteases inhibitors in order to improve the nutritional value of seeds or to increase PI content for tolerance to stored grain pests.

Published in	Advances in Biochemistry (Volume 7, Issue 1)
DOI	10.11648/j.ab.20190701.14
Page(s)	15-21
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2019. Published by Science Publishing Group

Keywords

Cowpea, Trypsin, Chymotrypsin, Genotypes

References

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Pierre Alexandre Eric Djifaby Sombié, Ahmed Yacouba Coulibaly, Adama Hilou, Martin Kiendrebéogo. (2019). Influence of Different Cowpea (Vigna unguiculata (L.) Walp.) Genotypes from Burkina Faso on Proteases Inhibition. Advances in Biochemistry, 7(1), 15-21. https://doi.org/10.11648/j.ab.20190701.14

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ACS Style

Pierre Alexandre Eric Djifaby Sombié; Ahmed Yacouba Coulibaly; Adama Hilou; Martin Kiendrebéogo. Influence of Different Cowpea (Vigna unguiculata (L.) Walp.) Genotypes from Burkina Faso on Proteases Inhibition. Adv. Biochem. 2019, 7(1), 15-21. doi: 10.11648/j.ab.20190701.14

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AMA Style

Pierre Alexandre Eric Djifaby Sombié, Ahmed Yacouba Coulibaly, Adama Hilou, Martin Kiendrebéogo. Influence of Different Cowpea (Vigna unguiculata (L.) Walp.) Genotypes from Burkina Faso on Proteases Inhibition. Adv Biochem. 2019;7(1):15-21. doi: 10.11648/j.ab.20190701.14

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@article{10.11648/j.ab.20190701.14,
  author = {Pierre Alexandre Eric Djifaby Sombié and Ahmed Yacouba Coulibaly and Adama Hilou and Martin Kiendrebéogo},
  title = {Influence of Different Cowpea (Vigna unguiculata (L.) Walp.) Genotypes from Burkina Faso on Proteases Inhibition},
  journal = {Advances in Biochemistry},
  volume = {7},
  number = {1},
  pages = {15-21},
  doi = {10.11648/j.ab.20190701.14},
  url = {https://doi.org/10.11648/j.ab.20190701.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ab.20190701.14},
  abstract = {Cowpea is an important protein crop widespread in Africa. The purpose of this research was to determine the content of trypsin and chymotrypsin inhibitors in different genotypes of cowpea seeds. Trypsin percentage inhibition showed about 13.5-fold variation (5.12% ± 1.47 to 70.52% ± 6.58) between the cowpea genotypes respectively for KVx 421-2J and Kondèsyoungo local. The chymotrypsin inhibitory activity varies among cowpea genotypes from 21.19 to 76.94%. The highest percent inhibitory activity was exhibited by KVx 396-4-5-2D on chymotrypsin. This study also showed significant correlations between type of cowpea genotype and trypsin inhibition potential and also between colour and chymotrypsin inhibition potential of cowpea seeds. Kondèsyoungo local, a landraceae genotype exhibited the high potential to inhibit the trypsin enzyme. Coloured seeds of cowpea genotypes possess higher percentage inhibition of chymotrypsin than the colourless ones (p < 0.05). The calculated mean of trypsin and chymotrypsin inhibition activities showed that Labagela local genotype possess the highest inhibition activity of both protease enzymes. The PCA components analysis and the dendrogram performed basis on the protease inhibitory activities divided the thirty-one genotypes of cowpea used in this study into three classes. The results presented in this work can contribute greatly to the planning of a cowpea breeding program aimed at reducing the content of proteases inhibitors in order to improve the nutritional value of seeds or to increase PI content for tolerance to stored grain pests.},
 year = {2019}
}

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TY - JOUR
T1 - Influence of Different Cowpea (Vigna unguiculata (L.) Walp.) Genotypes from Burkina Faso on Proteases Inhibition
AU - Pierre Alexandre Eric Djifaby Sombié
AU - Ahmed Yacouba Coulibaly
AU - Adama Hilou
AU - Martin Kiendrebéogo
Y1 - 2019/06/11
PY - 2019
N1 - https://doi.org/10.11648/j.ab.20190701.14
DO - 10.11648/j.ab.20190701.14
T2 - Advances in Biochemistry
JF - Advances in Biochemistry
JO - Advances in Biochemistry
SP - 15
EP - 21
PB - Science Publishing Group
SN - 2329-0862
UR - https://doi.org/10.11648/j.ab.20190701.14
AB - Cowpea is an important protein crop widespread in Africa. The purpose of this research was to determine the content of trypsin and chymotrypsin inhibitors in different genotypes of cowpea seeds. Trypsin percentage inhibition showed about 13.5-fold variation (5.12% ± 1.47 to 70.52% ± 6.58) between the cowpea genotypes respectively for KVx 421-2J and Kondèsyoungo local. The chymotrypsin inhibitory activity varies among cowpea genotypes from 21.19 to 76.94%. The highest percent inhibitory activity was exhibited by KVx 396-4-5-2D on chymotrypsin. This study also showed significant correlations between type of cowpea genotype and trypsin inhibition potential and also between colour and chymotrypsin inhibition potential of cowpea seeds. Kondèsyoungo local, a landraceae genotype exhibited the high potential to inhibit the trypsin enzyme. Coloured seeds of cowpea genotypes possess higher percentage inhibition of chymotrypsin than the colourless ones (p < 0.05). The calculated mean of trypsin and chymotrypsin inhibition activities showed that Labagela local genotype possess the highest inhibition activity of both protease enzymes. The PCA components analysis and the dendrogram performed basis on the protease inhibitory activities divided the thirty-one genotypes of cowpea used in this study into three classes. The results presented in this work can contribute greatly to the planning of a cowpea breeding program aimed at reducing the content of proteases inhibitors in order to improve the nutritional value of seeds or to increase PI content for tolerance to stored grain pests.
VL - 7
IS - 1
ER -

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Author Information

Pierre Alexandre Eric Djifaby Sombié

Department of Crop Production, Institute of Environment and Agricultural Research (INERA), Ouagadougou, Burkina Faso
Ahmed Yacouba Coulibaly

Unit of formation and Research in Sciences and Technology, University Norbert Zongo, Koudougou, Burkina Faso
Adama Hilou

Laboratory of Biochemistry and Chemistry Applied (LABIOCA), Department of Biochemistry-Microbiology, University of Ouagadougou, Ouagadougou, Burkina Faso
Martin Kiendrebéogo

Laboratory of Biochemistry and Chemistry Applied (LABIOCA), Department of Biochemistry-Microbiology, University of Ouagadougou, Ouagadougou, Burkina Faso

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Pierre Alexandre Eric Djifaby Sombié, Ahmed Yacouba Coulibaly, Adama Hilou, Martin Kiendrebéogo. (2019). Influence of Different Cowpea (Vigna unguiculata (L.) Walp.) Genotypes from Burkina Faso on Proteases Inhibition. Advances in Biochemistry, 7(1), 15-21. https://doi.org/10.11648/j.ab.20190701.14

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Pierre Alexandre Eric Djifaby Sombié; Ahmed Yacouba Coulibaly; Adama Hilou; Martin Kiendrebéogo. Influence of Different Cowpea (Vigna unguiculata (L.) Walp.) Genotypes from Burkina Faso on Proteases Inhibition. Adv. Biochem. 2019, 7(1), 15-21. doi: 10.11648/j.ab.20190701.14

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AMA Style

Pierre Alexandre Eric Djifaby Sombié, Ahmed Yacouba Coulibaly, Adama Hilou, Martin Kiendrebéogo. Influence of Different Cowpea (Vigna unguiculata (L.) Walp.) Genotypes from Burkina Faso on Proteases Inhibition. Adv Biochem. 2019;7(1):15-21. doi: 10.11648/j.ab.20190701.14

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@article{10.11648/j.ab.20190701.14,
  author = {Pierre Alexandre Eric Djifaby Sombié and Ahmed Yacouba Coulibaly and Adama Hilou and Martin Kiendrebéogo},
  title = {Influence of Different Cowpea (Vigna unguiculata (L.) Walp.) Genotypes from Burkina Faso on Proteases Inhibition},
  journal = {Advances in Biochemistry},
  volume = {7},
  number = {1},
  pages = {15-21},
  doi = {10.11648/j.ab.20190701.14},
  url = {https://doi.org/10.11648/j.ab.20190701.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ab.20190701.14},
  abstract = {Cowpea is an important protein crop widespread in Africa. The purpose of this research was to determine the content of trypsin and chymotrypsin inhibitors in different genotypes of cowpea seeds. Trypsin percentage inhibition showed about 13.5-fold variation (5.12% ± 1.47 to 70.52% ± 6.58) between the cowpea genotypes respectively for KVx 421-2J and Kondèsyoungo local. The chymotrypsin inhibitory activity varies among cowpea genotypes from 21.19 to 76.94%. The highest percent inhibitory activity was exhibited by KVx 396-4-5-2D on chymotrypsin. This study also showed significant correlations between type of cowpea genotype and trypsin inhibition potential and also between colour and chymotrypsin inhibition potential of cowpea seeds. Kondèsyoungo local, a landraceae genotype exhibited the high potential to inhibit the trypsin enzyme. Coloured seeds of cowpea genotypes possess higher percentage inhibition of chymotrypsin than the colourless ones (p < 0.05). The calculated mean of trypsin and chymotrypsin inhibition activities showed that Labagela local genotype possess the highest inhibition activity of both protease enzymes. The PCA components analysis and the dendrogram performed basis on the protease inhibitory activities divided the thirty-one genotypes of cowpea used in this study into three classes. The results presented in this work can contribute greatly to the planning of a cowpea breeding program aimed at reducing the content of proteases inhibitors in order to improve the nutritional value of seeds or to increase PI content for tolerance to stored grain pests.},
 year = {2019}
}

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