Antibiotics are of great importance in medicine, that are harmful to the growth and metabolic activities of bacteria. Ever increasing search is going on in the hope that agents’ superior to these and other antibiotics now in use might be isolated. Present study seeks to screen bacteria with antibiotic producing potentials from termite mounds. Eight (8) termite mound samples were collected from the study sites (Shamawa, Dundaye, G/Yaro and Gumburawa), processed and bacteria were isolated and identified using culturing methods, gram staining and biochemical characterization methods. The isolates were further tested for antibiotic production using crowded plate techniques on muellar-hilton agar. Morphological and cultural studies shows that twenty gram positive and six gram negative bacteria species were identified namely Bacillus spp, Citrobacter freundii, Enterococcus cloacae, Enterococcus faecalis, E. coli, K-pneumoniae, Pseudomonas spp, Staph aureus, Staph lentus and Staph ureae. Eleven bacteria species out of the fourteen isolates showed antibiotic production activity against Staphylococcus epidermidis and 3 were not active against the test organism. These include E. coli and Pseudomonas spp. The zone of inhibition formed are Bacillus cereus 4 mm, Bacillus subtilis 5 mm, Bacillus megaterium 2 mm, Citrobacter freundii 4 mm, Enterococcus cloacae 2 mm, and Enterococcus faecalis 2 mm respectively. The result of this research indicates that termites mound may be used as a source of antibiotic producing bacteria.
| Published in | Advances in Biochemistry (Volume 9, Issue 3) |
| DOI | 10.11648/j.ab.20210903.14 |
| Page(s) | 56-59 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
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Copyright © The Author(s), 2021. Published by Science Publishing Group |
Bacillus Spp, Pseudomonas Spp, Staphylococcus Spp, Enterococus Spp
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APA Style
Salisu Hussaini, Muhammad Ummulkusum, Manga Shuaibu Bala, Maimuna Abdulrahman Ashafa. (2021). Isolation, Identification and Screening of Bacteria with Antibiotic Production Potential from Termite Mounds. Advances in Biochemistry, 9(3), 56-59. https://doi.org/10.11648/j.ab.20210903.14
ACS Style
Salisu Hussaini; Muhammad Ummulkusum; Manga Shuaibu Bala; Maimuna Abdulrahman Ashafa. Isolation, Identification and Screening of Bacteria with Antibiotic Production Potential from Termite Mounds. Adv. Biochem. 2021, 9(3), 56-59. doi: 10.11648/j.ab.20210903.14
AMA Style
Salisu Hussaini, Muhammad Ummulkusum, Manga Shuaibu Bala, Maimuna Abdulrahman Ashafa. Isolation, Identification and Screening of Bacteria with Antibiotic Production Potential from Termite Mounds. Adv Biochem. 2021;9(3):56-59. doi: 10.11648/j.ab.20210903.14
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Download@article{10.11648/j.ab.20210903.14,
author = {Salisu Hussaini and Muhammad Ummulkusum and Manga Shuaibu Bala and Maimuna Abdulrahman Ashafa},
title = {Isolation, Identification and Screening of Bacteria with Antibiotic Production Potential from Termite Mounds},
journal = {Advances in Biochemistry},
volume = {9},
number = {3},
pages = {56-59},
doi = {10.11648/j.ab.20210903.14},
url = {https://doi.org/10.11648/j.ab.20210903.14},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ab.20210903.14},
abstract = {Antibiotics are of great importance in medicine, that are harmful to the growth and metabolic activities of bacteria. Ever increasing search is going on in the hope that agents’ superior to these and other antibiotics now in use might be isolated. Present study seeks to screen bacteria with antibiotic producing potentials from termite mounds. Eight (8) termite mound samples were collected from the study sites (Shamawa, Dundaye, G/Yaro and Gumburawa), processed and bacteria were isolated and identified using culturing methods, gram staining and biochemical characterization methods. The isolates were further tested for antibiotic production using crowded plate techniques on muellar-hilton agar. Morphological and cultural studies shows that twenty gram positive and six gram negative bacteria species were identified namely Bacillus spp, Citrobacter freundii, Enterococcus cloacae, Enterococcus faecalis, E. coli, K-pneumoniae, Pseudomonas spp, Staph aureus, Staph lentus and Staph ureae. Eleven bacteria species out of the fourteen isolates showed antibiotic production activity against Staphylococcus epidermidis and 3 were not active against the test organism. These include E. coli and Pseudomonas spp. The zone of inhibition formed are Bacillus cereus 4 mm, Bacillus subtilis 5 mm, Bacillus megaterium 2 mm, Citrobacter freundii 4 mm, Enterococcus cloacae 2 mm, and Enterococcus faecalis 2 mm respectively. The result of this research indicates that termites mound may be used as a source of antibiotic producing bacteria.},
year = {2021}
}
TY - JOUR T1 - Isolation, Identification and Screening of Bacteria with Antibiotic Production Potential from Termite Mounds AU - Salisu Hussaini AU - Muhammad Ummulkusum AU - Manga Shuaibu Bala AU - Maimuna Abdulrahman Ashafa Y1 - 2021/08/24 PY - 2021 N1 - https://doi.org/10.11648/j.ab.20210903.14 DO - 10.11648/j.ab.20210903.14 T2 - Advances in Biochemistry JF - Advances in Biochemistry JO - Advances in Biochemistry SP - 56 EP - 59 PB - Science Publishing Group SN - 2329-0862 UR - https://doi.org/10.11648/j.ab.20210903.14 AB - Antibiotics are of great importance in medicine, that are harmful to the growth and metabolic activities of bacteria. Ever increasing search is going on in the hope that agents’ superior to these and other antibiotics now in use might be isolated. Present study seeks to screen bacteria with antibiotic producing potentials from termite mounds. Eight (8) termite mound samples were collected from the study sites (Shamawa, Dundaye, G/Yaro and Gumburawa), processed and bacteria were isolated and identified using culturing methods, gram staining and biochemical characterization methods. The isolates were further tested for antibiotic production using crowded plate techniques on muellar-hilton agar. Morphological and cultural studies shows that twenty gram positive and six gram negative bacteria species were identified namely Bacillus spp, Citrobacter freundii, Enterococcus cloacae, Enterococcus faecalis, E. coli, K-pneumoniae, Pseudomonas spp, Staph aureus, Staph lentus and Staph ureae. Eleven bacteria species out of the fourteen isolates showed antibiotic production activity against Staphylococcus epidermidis and 3 were not active against the test organism. These include E. coli and Pseudomonas spp. The zone of inhibition formed are Bacillus cereus 4 mm, Bacillus subtilis 5 mm, Bacillus megaterium 2 mm, Citrobacter freundii 4 mm, Enterococcus cloacae 2 mm, and Enterococcus faecalis 2 mm respectively. The result of this research indicates that termites mound may be used as a source of antibiotic producing bacteria. VL - 9 IS - 3 ER -
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