Information on genetic diversity with respect to biochemical characteristics is very important for improving tea quality. Thirteen introduced tea clones were characterized at Jimma agricultural research center (Melko and Gera research stations) with the aim of identifying the diversity of tea clones based on biochemical traits. The study was conducted in triplicate of RCBD during the 2017/18 crop season. Data on biochemical traits such as total polyphenols, total antioxidants, β-carotene, ascorbic acid, leaf water content, chlorophyll content, and photosynthetic efficiency were recorded. Cluster analysis showed that the tea clones were classified into four groups, indicating the presence of variability among the examined clones. The maximum distance was observed between clusters III and IV (1216) whereas, the minimum distance (373.39) was between clusters II and IV. The results of principal components analysis exhibited that the first three principal components contributed for 77.22% diversity among the tea clones. In general, the study showed existing of difference among tea clones on considered biochemical parameters. Nevertheless, high biochemical variation among clones does not only a assurance for a high genetic difference; hence, molecular research need to be complemented as corresponding to biochemical studies.
Published in | Advances in Biochemistry (Volume 11, Issue 3) |
DOI | 10.11648/j.ab.20231103.12 |
Page(s) | 34-40 |
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This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
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Copyright © The Author(s), 2023. Published by Science Publishing Group |
Biochemical, Inter-Cluster, Diversity, Multivariate, Quality
[1] | Phong, N. H., W. Pongnak, K. Soytong, S. Poeaim, A. Poeaim, Diversity of tea (Camellia sinensis) grown in Vietnam based on morphological characteristics and inter-primer binding sites (iPBS) marker. Inter. Jour. of Agri. Bio., 2016; 18: 385-392. |
[2] | Mohammedsani Z., Melaku A., Kassaye T. Multivariate Analysis of Tea (Camellia sinensis (L.) O. Kuntze) Clones on Morphological Traits in Southwestern Ethiopia; International Journal of Plant Breeding and Crop Science 2021; 8: 1080-1087. |
[3] | Wight, W. Nomenclature and classification of the tea plant. Nature, 1959; 183: 1726-1728. |
[4] | Mondal, T. K., A. Bhattacharya, M. Laxmikumaran, P. S. Ahuj. Recent advances of tea (Camellia sinensis) biotechnology - Review of Plant Biotechnology and Applied Genetics. Jour. of Plant Cell, Tis. and Org. Cult., 2004; 76: 195-254. |
[5] | Eden, T. The development of tea culture. Pp 1-4. In: Eden, T. (Ed) Tea. Longman, London, U. K. 1958. |
[6] | Martin, L. C, Tea: The Drink that Changed the World. Tuttle Publishing. 2007; 7-8p. |
[7] | Mondal, T. K. Camellia. Pp 15-40. In: Kole C (eds.) Wild crop relatives: genomic and breeding resources. Springer-Verlag, Heidelberg, 2011. |
[8] | Seurei, P. Tea improvement in Kenya: a review. Tea, 1996; 17: 76-81. |
[9] | Mukhopadhyay, M., K. M. Tapan, K. C. Pradeep. Biotechnological advances in tea (Camellia sinensis [L.]O. Kuntze): a review; Plant Cell Rep. 2015; 1-34p. |
[10] | Shannon, E., A. K. Jaiswal, N. Abu-Ghannam. Polyphenolic content and anti-oxidant capacity of white, green, black, and herbal teas: a kinetic study; Food Res., 2018; 2: 1-11. |
[11] | Koech, K. R., F. N. Wachira, R. M. Ngure, I. A. Orina, J. K. Wanyoko, C. Bii, S. M. Karori. Antifungal activity of crude tea extracts. Afric. Jour. of Agric. Res., 2013; 8: 2086-2089. |
[12] | Khan, N., H. Mukhtar. Tea Polyphenols for health promotion. Life Sci., 2007; 81: 519-533. |
[13] | Karori, S. M., F. N. Wachira, J. K. Wanyoko, R. M. Ngure. Anti-oxidant capacity of different types of tea products. Afric. Jour. of Biotech., 2007; 6: 2287-2296. |
[14] | Kerio, L. C., F. N. Wachira, J. K. Wanyoko, M. K. Rotich. Total polyphenols, catechin profiles and anti-oxidant activity of tea products from purple leaf coloured tea cultivars. Food chemis., 2013; 136: 1405-1413. |
[15] | Tony, K. M. Drought responsive genes in tea cultivars grown in Kenya a; Thesis Submitted to the Graduate School in Partial Fulfillment of the Requirements for the Award of Master of Science Degree in Biochemistry of Egerton University. 2013; 16p. |
[16] | Lu, M. J., S. C. Chu, L. Yan, C. Chen. Effect of tannase treatment on protein tannin aggregation and sensory attributes of green tea infusion. LWT-Food Sci. and Tech. 2009; 42: 338-342. |
[17] | IPGRI. Descriptors for Tea (Camellia sinensis). International Plant Genetic Resources Institute, Rome, Italy; 1997. |
[18] | Kottawa-Arachchi, J. D., M. T. K. Gunasekare, M. A. B. Ranatunga, P. A. N. Punyasiri, L. Jayasinghe,. Use of biochemical compounds in tea germplasm characterization and its implications in tea breeding in Sri Lanka; J. Natn. Sci. Found. Sri., 2013; 41: 309-318. |
[19] | Solomon. T, B. Gezahegn, D. Abnet, G. Biyensa, T. Wondimu, S. Meseret, A. Teshome. Participatory Rural Appraisal Report: Gera Woreda, West Oromia Region; Cascape Working Paper; capacity building for scaling up of evidence based best practices in agricultural production in Ethiopia; 2014; 20p. |
[20] | Ahmad, F., F. S. Hamid, S. Sarwar, S. Waheed, S. Aslam, U. Islam, S. Hussain, N. Ahmad, I. Ali. Effect of different pruning times on the yield of tea (camellia sinensis L.) under the climatic conditions of Mansehra Pakistan. Sarhad Jour. of Agric., 2014; 30: 305-309. |
[21] | Sowa, S., A. E. Kondo. Sailing on the" C": A Vitamin Titration with a Twist. J. Chem. Educ., 2003; 80: 550. |
[22] | Park, Y. W. Effect of freezing, thawing, drying and cooking on carotene retention in carrots, broccoli and spinach. J. Food Sci., 1987; 52: 1022-1025. |
[23] | Lu, Y., L. Y. Foo. Anti-oxidant and radical scavenging activities of polyphenols from apple pomace. Food chem., 2000; 68: 81-85. |
[24] | SAS. Statistical analysis System (Version 9.3) SAS Institute Cary, NC. USA, 2014. |
[25] | Mahalanobis, P. C. On the generalized distance in statistics. Jour. of Gen., 1936; 41: 159-193. |
[26] | Singh, R. K., B. D. Chaundry. Biometrical Methods in Quantitative Genetics Analysis. Kalyani Publishers, New Delhi., 1987; 318p. |
[27] | Piyasundara, J. H. N, M. T. K. Gunasekare, I. P. Wickramasinghe. Characterization of Tea (Camellia sinensis L.) Germplasm in Sri Lanka using Morphological Descriptors, S. L. J, Tea Sci., 2009; 74: 31-39. |
APA Style
Lemi Beksisa, Mohammedsani Zakir, Melaku Addisu. (2023). Multivariate Analysis of Tea (Camellia sinensis (L.) O. Kuntze) Clones on Biochemical Characters in Southwestern Ethiopia. Advances in Biochemistry, 11(3), 34-40. https://doi.org/10.11648/j.ab.20231103.12
ACS Style
Lemi Beksisa; Mohammedsani Zakir; Melaku Addisu. Multivariate Analysis of Tea (Camellia sinensis (L.) O. Kuntze) Clones on Biochemical Characters in Southwestern Ethiopia. Adv. Biochem. 2023, 11(3), 34-40. doi: 10.11648/j.ab.20231103.12
AMA Style
Lemi Beksisa, Mohammedsani Zakir, Melaku Addisu. Multivariate Analysis of Tea (Camellia sinensis (L.) O. Kuntze) Clones on Biochemical Characters in Southwestern Ethiopia. Adv Biochem. 2023;11(3):34-40. doi: 10.11648/j.ab.20231103.12
@article{10.11648/j.ab.20231103.12, author = {Lemi Beksisa and Mohammedsani Zakir and Melaku Addisu}, title = {Multivariate Analysis of Tea (Camellia sinensis (L.) O. Kuntze) Clones on Biochemical Characters in Southwestern Ethiopia}, journal = {Advances in Biochemistry}, volume = {11}, number = {3}, pages = {34-40}, doi = {10.11648/j.ab.20231103.12}, url = {https://doi.org/10.11648/j.ab.20231103.12}, eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ab.20231103.12}, abstract = {Information on genetic diversity with respect to biochemical characteristics is very important for improving tea quality. Thirteen introduced tea clones were characterized at Jimma agricultural research center (Melko and Gera research stations) with the aim of identifying the diversity of tea clones based on biochemical traits. The study was conducted in triplicate of RCBD during the 2017/18 crop season. Data on biochemical traits such as total polyphenols, total antioxidants, β-carotene, ascorbic acid, leaf water content, chlorophyll content, and photosynthetic efficiency were recorded. Cluster analysis showed that the tea clones were classified into four groups, indicating the presence of variability among the examined clones. The maximum distance was observed between clusters III and IV (1216) whereas, the minimum distance (373.39) was between clusters II and IV. The results of principal components analysis exhibited that the first three principal components contributed for 77.22% diversity among the tea clones. In general, the study showed existing of difference among tea clones on considered biochemical parameters. Nevertheless, high biochemical variation among clones does not only a assurance for a high genetic difference; hence, molecular research need to be complemented as corresponding to biochemical studies.}, year = {2023} }
TY - JOUR T1 - Multivariate Analysis of Tea (Camellia sinensis (L.) O. Kuntze) Clones on Biochemical Characters in Southwestern Ethiopia AU - Lemi Beksisa AU - Mohammedsani Zakir AU - Melaku Addisu Y1 - 2023/07/31 PY - 2023 N1 - https://doi.org/10.11648/j.ab.20231103.12 DO - 10.11648/j.ab.20231103.12 T2 - Advances in Biochemistry JF - Advances in Biochemistry JO - Advances in Biochemistry SP - 34 EP - 40 PB - Science Publishing Group SN - 2329-0862 UR - https://doi.org/10.11648/j.ab.20231103.12 AB - Information on genetic diversity with respect to biochemical characteristics is very important for improving tea quality. Thirteen introduced tea clones were characterized at Jimma agricultural research center (Melko and Gera research stations) with the aim of identifying the diversity of tea clones based on biochemical traits. The study was conducted in triplicate of RCBD during the 2017/18 crop season. Data on biochemical traits such as total polyphenols, total antioxidants, β-carotene, ascorbic acid, leaf water content, chlorophyll content, and photosynthetic efficiency were recorded. Cluster analysis showed that the tea clones were classified into four groups, indicating the presence of variability among the examined clones. The maximum distance was observed between clusters III and IV (1216) whereas, the minimum distance (373.39) was between clusters II and IV. The results of principal components analysis exhibited that the first three principal components contributed for 77.22% diversity among the tea clones. In general, the study showed existing of difference among tea clones on considered biochemical parameters. Nevertheless, high biochemical variation among clones does not only a assurance for a high genetic difference; hence, molecular research need to be complemented as corresponding to biochemical studies. VL - 11 IS - 3 ER -