LPS Treatment and Exposure to PEMF induce Cell Death and Change in Secretory Activity of HMVEC-Bd with MM6 Cocultutre

Kaszuba-Zwoińska Jolanta; Chorobik Paulina; Nowak Bernadeta; Ziomber Agata; Juszczak Kajetan; Zaraska Wiesław; Thor Piotr

doi:doi:10.11648/j.abb.20140203.12

LPS Treatment and Exposure to PEMF induce Cell Death and Change in Secretory Activity of HMVEC-Bd with MM6 Cocultutre

Kaszuba-Zwoińska Jolanta, Chorobik Paulina, Nowak Bernadeta, Ziomber Agata, Juszczak Kajetan, Zaraska Wiesław, Thor Piotr

Published in Advances in Bioscience and Bioengineering (Volume 2, Issue 3)

Received: 27 August 2014 Accepted: 11 September 2014 Published: 30 September 2014

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Abstract

Objective: Present studies are aimed to elucidate that pulsed electromagnetic field (PEMF) influences cell death parameters and cellular interactions in coculture model in response to inflammatory stimulus like E.coli endotoxin. Methods: We hypothesized that PEMF exposure will affect cell death rate in the experimental coculture model, composed of the human bladder microvascular endothelial cell line (HMEVEC-Bd) and MonoMac6 (MM6) cells previously activated with LPS, and exposed to PEMF (7Hz, 30mT) for three times with 24h intervals. Following the last electromagnetic exposure, we measured viability of cocultured and cultured cells by annexin V (AnV) - propidium iodide (PI) flow cytometry staining procedure to evaluate cell death parameters. The level of proinflammatory cytokine, cell adhesion molecules and vascular endothelial growth factor (IL-8, ICAM-1 and VEGF-A) was estimated by ELISA method in coculture and cell culture collected supernatants. Results: PEMF exposure of HMVEC-Bd and MM6 coculture caused decrease of measured cell death parameters (early and late apoptosis as well as necrosis) and diminished production of some inflammatory agents released in response to LPS activation, comparing to not stimulated with PEMF controls. Conclussion: Obtained results confirmed our hypothesis and showed out that PEMF exposure of HMVEC-Bd & MM6 coculture previously activated with LPS exerted an anti-inflammatory effect.

Published in	Advances in Bioscience and Bioengineering (Volume 2, Issue 3)
DOI	10.11648/j.abb.20140203.12
Page(s)	30-36
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2014. Published by Science Publishing Group

Keywords

Urothelial Cell Line, Monocytic Cell Line, Coculture, Apoptosis, Necrosis, Flow Cytometry, ICAM-1, IL-8,Pulsed Electromagnetic Field, VEGF-A

References

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APA Style

Kaszuba-Zwoińska Jolanta, Chorobik Paulina, Nowak Bernadeta, Ziomber Agata, Juszczak Kajetan, et al. (2014). LPS Treatment and Exposure to PEMF induce Cell Death and Change in Secretory Activity of HMVEC-Bd with MM6 Cocultutre. Advances in Bioscience and Bioengineering, 2(3), 30-36. https://doi.org/10.11648/j.abb.20140203.12

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ACS Style

Kaszuba-Zwoińska Jolanta; Chorobik Paulina; Nowak Bernadeta; Ziomber Agata; Juszczak Kajetan, et al. LPS Treatment and Exposure to PEMF induce Cell Death and Change in Secretory Activity of HMVEC-Bd with MM6 Cocultutre. Adv. BioSci. Bioeng. 2014, 2(3), 30-36. doi: 10.11648/j.abb.20140203.12

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AMA Style

Kaszuba-Zwoińska Jolanta, Chorobik Paulina, Nowak Bernadeta, Ziomber Agata, Juszczak Kajetan, et al. LPS Treatment and Exposure to PEMF induce Cell Death and Change in Secretory Activity of HMVEC-Bd with MM6 Cocultutre. Adv BioSci Bioeng. 2014;2(3):30-36. doi: 10.11648/j.abb.20140203.12

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@article{10.11648/j.abb.20140203.12,
  author = {Kaszuba-Zwoińska Jolanta and Chorobik Paulina and Nowak Bernadeta and Ziomber Agata and Juszczak Kajetan and Zaraska Wiesław and Thor Piotr},
  title = {LPS Treatment and Exposure to PEMF induce Cell Death and Change in Secretory Activity of HMVEC-Bd with MM6 Cocultutre},
  journal = {Advances in Bioscience and Bioengineering},
  volume = {2},
  number = {3},
  pages = {30-36},
  doi = {10.11648/j.abb.20140203.12},
  url = {https://doi.org/10.11648/j.abb.20140203.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.abb.20140203.12},
  abstract = {Objective: Present studies are aimed to elucidate that pulsed electromagnetic field (PEMF) influences cell death parameters and cellular interactions in coculture model in response to inflammatory stimulus like E.coli endotoxin. Methods: We hypothesized that PEMF exposure will affect cell death rate in the experimental coculture model, composed of the human bladder microvascular endothelial cell line (HMEVEC-Bd) and MonoMac6 (MM6) cells previously activated with LPS, and exposed to PEMF (7Hz, 30mT) for three times with 24h intervals. Following the last electromagnetic exposure, we measured viability of cocultured and cultured cells by annexin V (AnV) - propidium iodide (PI) flow cytometry staining procedure to evaluate cell death parameters. The level of proinflammatory cytokine, cell adhesion molecules and vascular endothelial growth factor (IL-8, ICAM-1 and VEGF-A) was estimated by ELISA method in coculture and cell culture collected supernatants. Results: PEMF exposure of HMVEC-Bd and MM6 coculture caused decrease of measured cell death parameters (early and late apoptosis as well as necrosis) and diminished production of some inflammatory agents released in response to LPS activation, comparing to not stimulated with PEMF controls. Conclussion: Obtained results confirmed our hypothesis and showed out that PEMF exposure of HMVEC-Bd & MM6 coculture previously activated with LPS exerted an anti-inflammatory effect.},
 year = {2014}
}

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TY  - JOUR
T1  - LPS Treatment and Exposure to PEMF induce Cell Death and Change in Secretory Activity of HMVEC-Bd with MM6 Cocultutre
AU  - Kaszuba-Zwoińska Jolanta
AU  - Chorobik Paulina
AU  - Nowak Bernadeta
AU  - Ziomber Agata
AU  - Juszczak Kajetan
AU  - Zaraska Wiesław
AU  - Thor Piotr
Y1  - 2014/09/30
PY  - 2014
N1  - https://doi.org/10.11648/j.abb.20140203.12
DO  - 10.11648/j.abb.20140203.12
T2  - Advances in Bioscience and Bioengineering
JF  - Advances in Bioscience and Bioengineering
JO  - Advances in Bioscience and Bioengineering
SP  - 30
EP  - 36
PB  - Science Publishing Group
SN  - 2330-4162
UR  - https://doi.org/10.11648/j.abb.20140203.12
AB  - Objective: Present studies are aimed to elucidate that pulsed electromagnetic field (PEMF) influences cell death parameters and cellular interactions in coculture model in response to inflammatory stimulus like E.coli endotoxin. Methods: We hypothesized that PEMF exposure will affect cell death rate in the experimental coculture model, composed of the human bladder microvascular endothelial cell line (HMEVEC-Bd) and MonoMac6 (MM6) cells previously activated with LPS, and exposed to PEMF (7Hz, 30mT) for three times with 24h intervals. Following the last electromagnetic exposure, we measured viability of cocultured and cultured cells by annexin V (AnV) - propidium iodide (PI) flow cytometry staining procedure to evaluate cell death parameters. The level of proinflammatory cytokine, cell adhesion molecules and vascular endothelial growth factor (IL-8, ICAM-1 and VEGF-A) was estimated by ELISA method in coculture and cell culture collected supernatants. Results: PEMF exposure of HMVEC-Bd and MM6 coculture caused decrease of measured cell death parameters (early and late apoptosis as well as necrosis) and diminished production of some inflammatory agents released in response to LPS activation, comparing to not stimulated with PEMF controls. Conclussion: Obtained results confirmed our hypothesis and showed out that PEMF exposure of HMVEC-Bd & MM6 coculture previously activated with LPS exerted an anti-inflammatory effect.
VL  - 2
IS  - 3
ER  -

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Author Information

Kaszuba-Zwoińska Jolanta

Department of Pathophysiology, Medical College, Jagiellonian University, Cracow, Poland
Chorobik Paulina

Department of Immunology, Medical College, Jagiellonian University, Cracow, Poland
Nowak Bernadeta

Department of Immunology, Medical College, Jagiellonian University, Cracow, Poland
Ziomber Agata

Department of Pathophysiology, Medical College, Jagiellonian University, Cracow, Poland
Juszczak Kajetan

Department of Pathophysiology, Medical College, Jagiellonian University, Cracow, Poland
Zaraska Wiesław
Thor Piotr

Department of Pathophysiology, Medical College, Jagiellonian University, Cracow, Poland

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APA Style

Kaszuba-Zwoińska Jolanta, Chorobik Paulina, Nowak Bernadeta, Ziomber Agata, Juszczak Kajetan, et al. (2014). LPS Treatment and Exposure to PEMF induce Cell Death and Change in Secretory Activity of HMVEC-Bd with MM6 Cocultutre. Advances in Bioscience and Bioengineering, 2(3), 30-36. https://doi.org/10.11648/j.abb.20140203.12

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ACS Style

Kaszuba-Zwoińska Jolanta; Chorobik Paulina; Nowak Bernadeta; Ziomber Agata; Juszczak Kajetan, et al. LPS Treatment and Exposure to PEMF induce Cell Death and Change in Secretory Activity of HMVEC-Bd with MM6 Cocultutre. Adv. BioSci. Bioeng. 2014, 2(3), 30-36. doi: 10.11648/j.abb.20140203.12

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AMA Style

Kaszuba-Zwoińska Jolanta, Chorobik Paulina, Nowak Bernadeta, Ziomber Agata, Juszczak Kajetan, et al. LPS Treatment and Exposure to PEMF induce Cell Death and Change in Secretory Activity of HMVEC-Bd with MM6 Cocultutre. Adv BioSci Bioeng. 2014;2(3):30-36. doi: 10.11648/j.abb.20140203.12

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@article{10.11648/j.abb.20140203.12,
  author = {Kaszuba-Zwoińska Jolanta and Chorobik Paulina and Nowak Bernadeta and Ziomber Agata and Juszczak Kajetan and Zaraska Wiesław and Thor Piotr},
  title = {LPS Treatment and Exposure to PEMF induce Cell Death and Change in Secretory Activity of HMVEC-Bd with MM6 Cocultutre},
  journal = {Advances in Bioscience and Bioengineering},
  volume = {2},
  number = {3},
  pages = {30-36},
  doi = {10.11648/j.abb.20140203.12},
  url = {https://doi.org/10.11648/j.abb.20140203.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.abb.20140203.12},
  abstract = {Objective: Present studies are aimed to elucidate that pulsed electromagnetic field (PEMF) influences cell death parameters and cellular interactions in coculture model in response to inflammatory stimulus like E.coli endotoxin. Methods: We hypothesized that PEMF exposure will affect cell death rate in the experimental coculture model, composed of the human bladder microvascular endothelial cell line (HMEVEC-Bd) and MonoMac6 (MM6) cells previously activated with LPS, and exposed to PEMF (7Hz, 30mT) for three times with 24h intervals. Following the last electromagnetic exposure, we measured viability of cocultured and cultured cells by annexin V (AnV) - propidium iodide (PI) flow cytometry staining procedure to evaluate cell death parameters. The level of proinflammatory cytokine, cell adhesion molecules and vascular endothelial growth factor (IL-8, ICAM-1 and VEGF-A) was estimated by ELISA method in coculture and cell culture collected supernatants. Results: PEMF exposure of HMVEC-Bd and MM6 coculture caused decrease of measured cell death parameters (early and late apoptosis as well as necrosis) and diminished production of some inflammatory agents released in response to LPS activation, comparing to not stimulated with PEMF controls. Conclussion: Obtained results confirmed our hypothesis and showed out that PEMF exposure of HMVEC-Bd & MM6 coculture previously activated with LPS exerted an anti-inflammatory effect.},
 year = {2014}
}

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TY  - JOUR
T1  - LPS Treatment and Exposure to PEMF induce Cell Death and Change in Secretory Activity of HMVEC-Bd with MM6 Cocultutre
AU  - Kaszuba-Zwoińska Jolanta
AU  - Chorobik Paulina
AU  - Nowak Bernadeta
AU  - Ziomber Agata
AU  - Juszczak Kajetan
AU  - Zaraska Wiesław
AU  - Thor Piotr
Y1  - 2014/09/30
PY  - 2014
N1  - https://doi.org/10.11648/j.abb.20140203.12
DO  - 10.11648/j.abb.20140203.12
T2  - Advances in Bioscience and Bioengineering
JF  - Advances in Bioscience and Bioengineering
JO  - Advances in Bioscience and Bioengineering
SP  - 30
EP  - 36
PB  - Science Publishing Group
SN  - 2330-4162
UR  - https://doi.org/10.11648/j.abb.20140203.12
AB  - Objective: Present studies are aimed to elucidate that pulsed electromagnetic field (PEMF) influences cell death parameters and cellular interactions in coculture model in response to inflammatory stimulus like E.coli endotoxin. Methods: We hypothesized that PEMF exposure will affect cell death rate in the experimental coculture model, composed of the human bladder microvascular endothelial cell line (HMEVEC-Bd) and MonoMac6 (MM6) cells previously activated with LPS, and exposed to PEMF (7Hz, 30mT) for three times with 24h intervals. Following the last electromagnetic exposure, we measured viability of cocultured and cultured cells by annexin V (AnV) - propidium iodide (PI) flow cytometry staining procedure to evaluate cell death parameters. The level of proinflammatory cytokine, cell adhesion molecules and vascular endothelial growth factor (IL-8, ICAM-1 and VEGF-A) was estimated by ELISA method in coculture and cell culture collected supernatants. Results: PEMF exposure of HMVEC-Bd and MM6 coculture caused decrease of measured cell death parameters (early and late apoptosis as well as necrosis) and diminished production of some inflammatory agents released in response to LPS activation, comparing to not stimulated with PEMF controls. Conclussion: Obtained results confirmed our hypothesis and showed out that PEMF exposure of HMVEC-Bd & MM6 coculture previously activated with LPS exerted an anti-inflammatory effect.
VL  - 2
IS  - 3
ER  -

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