The screening method for discovering new lipid synthesis inhibitors has not been well developed. Lipid synthesis genes are responsible for the synthesis of fatty acids in normal and tumor cells. Mortierella alpina(M. alpina) fungus has been found to produce large amounts of fatty acids because it possesses a full complement of lipid synthesis genes. We therefore hypothesized that M. alpina could be a good screening tool to find new compounds that inhibit fatty acid synthesis. We developed a M. alpina liquid culture based method to analyze the inhibitory effects of lipid synthesis inhibitors. We applied a color indicator method to monitor the oil production using some well documented lipid inhibitors (C75, Cerulenin) to verify the system, and analyzing pictures using image analysis software. The experimental drug nocodazole inhibited the lipid production in M. alpina almost to the same extent as the control inhibitors. Compared with biomass and protein levels, there was a profound effect on fatty acid level. We report the development of a fast and effective method for screening lipid synthesis inhibitors which can be used against obesity and cancer. This method can further be used to screen additional lipid and fatty acid synthesis inhibitors from natural compound libraries.
| Published in | Advances in Bioscience and Bioengineering (Volume 3, Issue 1) |
| DOI | 10.11648/j.abb.20150301.11 |
| Page(s) | 1-10 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2015. Published by Science Publishing Group |
Fatty Acid Synthase, Image Analysis Software, Lipid Synthesis Inhibitor, Mortierella alpina, Nocodazole, Screening System
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APA Style
Md. Shofiul Azam, Zhennan Gu, Haiqin Chen, Yong Q. Chen. (2015). Using Mortierella alpina as a Novel Platform for Lipid Synthesis Inhibitor Screening. Advances in Bioscience and Bioengineering, 3(1), 1-10. https://doi.org/10.11648/j.abb.20150301.11
ACS Style
Md. Shofiul Azam; Zhennan Gu; Haiqin Chen; Yong Q. Chen. Using Mortierella alpina as a Novel Platform for Lipid Synthesis Inhibitor Screening. Adv. BioSci. Bioeng. 2015, 3(1), 1-10. doi: 10.11648/j.abb.20150301.11
AMA Style
Md. Shofiul Azam, Zhennan Gu, Haiqin Chen, Yong Q. Chen. Using Mortierella alpina as a Novel Platform for Lipid Synthesis Inhibitor Screening. Adv BioSci Bioeng. 2015;3(1):1-10. doi: 10.11648/j.abb.20150301.11
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Download@article{10.11648/j.abb.20150301.11,
author = {Md. Shofiul Azam and Zhennan Gu and Haiqin Chen and Yong Q. Chen},
title = {Using Mortierella alpina as a Novel Platform for Lipid Synthesis Inhibitor Screening},
journal = {Advances in Bioscience and Bioengineering},
volume = {3},
number = {1},
pages = {1-10},
doi = {10.11648/j.abb.20150301.11},
url = {https://doi.org/10.11648/j.abb.20150301.11},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.abb.20150301.11},
abstract = {The screening method for discovering new lipid synthesis inhibitors has not been well developed. Lipid synthesis genes are responsible for the synthesis of fatty acids in normal and tumor cells. Mortierella alpina(M. alpina) fungus has been found to produce large amounts of fatty acids because it possesses a full complement of lipid synthesis genes. We therefore hypothesized that M. alpina could be a good screening tool to find new compounds that inhibit fatty acid synthesis. We developed a M. alpina liquid culture based method to analyze the inhibitory effects of lipid synthesis inhibitors. We applied a color indicator method to monitor the oil production using some well documented lipid inhibitors (C75, Cerulenin) to verify the system, and analyzing pictures using image analysis software. The experimental drug nocodazole inhibited the lipid production in M. alpina almost to the same extent as the control inhibitors. Compared with biomass and protein levels, there was a profound effect on fatty acid level. We report the development of a fast and effective method for screening lipid synthesis inhibitors which can be used against obesity and cancer. This method can further be used to screen additional lipid and fatty acid synthesis inhibitors from natural compound libraries.},
year = {2015}
}
TY - JOUR T1 - Using Mortierella alpina as a Novel Platform for Lipid Synthesis Inhibitor Screening AU - Md. Shofiul Azam AU - Zhennan Gu AU - Haiqin Chen AU - Yong Q. Chen Y1 - 2015/04/23 PY - 2015 N1 - https://doi.org/10.11648/j.abb.20150301.11 DO - 10.11648/j.abb.20150301.11 T2 - Advances in Bioscience and Bioengineering JF - Advances in Bioscience and Bioengineering JO - Advances in Bioscience and Bioengineering SP - 1 EP - 10 PB - Science Publishing Group SN - 2330-4162 UR - https://doi.org/10.11648/j.abb.20150301.11 AB - The screening method for discovering new lipid synthesis inhibitors has not been well developed. Lipid synthesis genes are responsible for the synthesis of fatty acids in normal and tumor cells. Mortierella alpina(M. alpina) fungus has been found to produce large amounts of fatty acids because it possesses a full complement of lipid synthesis genes. We therefore hypothesized that M. alpina could be a good screening tool to find new compounds that inhibit fatty acid synthesis. We developed a M. alpina liquid culture based method to analyze the inhibitory effects of lipid synthesis inhibitors. We applied a color indicator method to monitor the oil production using some well documented lipid inhibitors (C75, Cerulenin) to verify the system, and analyzing pictures using image analysis software. The experimental drug nocodazole inhibited the lipid production in M. alpina almost to the same extent as the control inhibitors. Compared with biomass and protein levels, there was a profound effect on fatty acid level. We report the development of a fast and effective method for screening lipid synthesis inhibitors which can be used against obesity and cancer. This method can further be used to screen additional lipid and fatty acid synthesis inhibitors from natural compound libraries. VL - 3 IS - 1 ER -
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