Design of Portable Time-Resolved Fluorometer

Chongde Zi; Junsheng Shi; Yonghang Tai; Huan Yang; Xicai Li

doi:doi:10.11648/j.abb.20160406.13

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Design of Portable Time-Resolved Fluorometer

Chongde Zi, Junsheng Shi, Yonghang Tai, Huan Yang, Xicai Li

Published in Advances in Bioscience and Bioengineering (Volume 4, Issue 6)

Received: 2 December 2016 Published: 5 December 2016

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Abstract

A miniaturization time-resolved fluorescence analyzer was designed based on immunofluorescence and embedded technology, this instrument can be utilized to detect the immunochromatographic strip to achieve quantitative inspection and analysis of analytes in human blood or body fluid. By mechanical scanning means point-by-point assay, using UV-LED irradiation the fluorescence labeled immunocomplex on the immunochromatographic strip, and the fluorescence was emitted after being excited. Fluorescent light passes through narrow-band optical filters and converted to electrical signal by Silicon photodiode (Si PIN). After amplification and analog-digital conversion, the signal is sent to the micro-controller STM32F103 for processing. The fluorescence intensity distribution of the reagent strip was obtained after the scan detection finished, and the concentration of the substance can be calculated based on the known standard curve. After testing, the instrument has a wide dynamic range of measurement. It not only has a reliable repeatability (CV<0.2%) performance in the detection of concentration larger than 100µg/mL, but also hasdemonstrate stable validate features (CV=2.6%) when the concentration only at 1µg/mL. It has commendably application prospects in point-of-care test detection (POCT).

Published in	Advances in Bioscience and Bioengineering (Volume 4, Issue 6)
DOI	10.11648/j.abb.20160406.13
Page(s)	79-84
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2016. Published by Science Publishing Group

Keywords

Time-Resolved, Fluorescence Immunoassay, TRFIA, Quantitative Detection, Si PIN, POCT

References

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[2]	Zhancheng Gao, “Application of the indirect immunofluorescence assay against specific antibodies in the diagnosis of infectious respiratory diseases,” Chinese Journal of Laboratory Medicine, vol. 35, 2012, pp. 697-700.
[3]	H. Déchaud, R. Bador, F. Claustrat, et al, “New approach to competitive lanthanide immunoassay: time-resolved fluoroimmunoassay of progesterone with labeled analyte,” Clinical Chemistry, vol. 34, 1988, pp. 501-504.
[4]	A. A. Oraevsky, S. L. Jacques, and F. K. Tittel, “Measurement of tissue optical properties by time-resolved detection of laser-induced transient stress,” Applied Optics, vol. 36, 1997, pp. 402-415.
[5]	Jian Shen, Deqiu Lin, and Jie Xu, “Present situation and progress of time-resolved fluoroimmunoassay,” Chinse Bulletin Life Sciences, vol.16, 2004, pp. 55-59.
[6]	Xuecheng Wu, Lin He, and Keyuan Zhou, “The Research and Clinical Application of Time- resolved Fluoroimmunoassay,” Medical Recapitulate, vol 12, 2006, pp. 434-436.
[7]	Q. Liao, X. A. L. Liu, and H. Guo, “The discussion of Time-resolved immunofluorescence method for determination of syphius antibody,” Chinese Journal of Laboratory Diagnosis, vol. 18, 2014, pp. 447-450.
[8]	Xiaoyu Fu, Feiyuan Wu, and Gang Chen, et al, “Feasibility analysis of quantitative detection on serum HBeAg/HBeAb by time-resolved immunofluorescence assay,” Journal of Central South University (Medical Science), vol. 41, 2016, pp. 852-855.
[9]	Jianfeng Hang, Ying Song Wu, and Ming Li, "New Progress in the Research on Time-resolved Fluoroimmunoassay." Journal of Tropical Medicine, vol. 4, 2004, pp. 340-343.
[10]	H. Siitari, I. Hemmilä, E. Soini, T. Lövgren, et al, “Detection of hepatitis B surface antigen using time-resolved fluoroimmunoassay,” Nature, vol. 301, 1983, pp. 258-260.
[11]	J. R. Lakowicz, “Emerging Biomedical Applications of Time-Resolved Fluorescence Spectroscopy,” Topics in Fluorescence Spectroscopy. Springer US, vol. 2136, 2002, pp. 1-19.
[12]	Poras, Herve, T. Ouimet, and M. C. Fournie-Zaluski, “substrates the fluorescence of which is suppressed, preparation thereof, and use thereof for identifying, detecting, and assaying legionella pneumophilia,” US20130203097. 2013.
[13]	P. B. Luppa, C. Müller, A. Schlichtiger, et al, “Point-of-care testing (POCT), pp. Current techniques and future perspectives,” Trac Trends in Analytical Chemistry, vol. 30, 2011, pp. 887-898.
[14]	R. H. Shyu, H. F. Shyu, H. W. Liu, “Colloidal gold-based immunochromatographic assay for detection of ricin,” Toxicon Official Journal of the International Society on Toxinology, vol.40, 2002, pp. 255-258.
[15]	B. B. Dzantiev, N. A. Byzova, A. E. Urusov, et al, "Immunochromatographic methods in food analysis,” Trac Trends in Analytical Chemistry, vol.55, 2014, pp. 81-93.
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APA Style

Chongde Zi, Junsheng Shi, Yonghang Tai, Huan Yang, Xicai Li. (2016). Design of Portable Time-Resolved Fluorometer. Advances in Bioscience and Bioengineering, 4(6), 79-84. https://doi.org/10.11648/j.abb.20160406.13

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ACS Style

Chongde Zi; Junsheng Shi; Yonghang Tai; Huan Yang; Xicai Li. Design of Portable Time-Resolved Fluorometer. Adv. BioSci. Bioeng. 2016, 4(6), 79-84. doi: 10.11648/j.abb.20160406.13

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AMA Style

Chongde Zi, Junsheng Shi, Yonghang Tai, Huan Yang, Xicai Li. Design of Portable Time-Resolved Fluorometer. Adv BioSci Bioeng. 2016;4(6):79-84. doi: 10.11648/j.abb.20160406.13

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@article{10.11648/j.abb.20160406.13,
  author = {Chongde Zi and Junsheng Shi and Yonghang Tai and Huan Yang and Xicai Li},
  title = {Design of Portable Time-Resolved Fluorometer},
  journal = {Advances in Bioscience and Bioengineering},
  volume = {4},
  number = {6},
  pages = {79-84},
  doi = {10.11648/j.abb.20160406.13},
  url = {https://doi.org/10.11648/j.abb.20160406.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.abb.20160406.13},
  abstract = {A miniaturization time-resolved fluorescence analyzer was designed based on immunofluorescence and embedded technology, this instrument can be utilized to detect the immunochromatographic strip to achieve quantitative inspection and analysis of analytes in human blood or body fluid. By mechanical scanning means point-by-point assay, using UV-LED irradiation the fluorescence labeled immunocomplex on the immunochromatographic strip, and the fluorescence was emitted after being excited. Fluorescent light passes through narrow-band optical filters and converted to electrical signal by Silicon photodiode (Si PIN). After amplification and analog-digital conversion, the signal is sent to the micro-controller STM32F103 for processing. The fluorescence intensity distribution of the reagent strip was obtained after the scan detection finished, and the concentration of the substance can be calculated based on the known standard curve. After testing, the instrument has a wide dynamic range of measurement. It not only has a reliable repeatability (CV<0.2%) performance in the detection of concentration larger than 100µg/mL, but also hasdemonstrate stable validate features (CV=2.6%) when the concentration only at 1µg/mL. It has commendably application prospects in point-of-care test detection (POCT).},
 year = {2016}
}

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TY  - JOUR
T1  - Design of Portable Time-Resolved Fluorometer
AU  - Chongde Zi
AU  - Junsheng Shi
AU  - Yonghang Tai
AU  - Huan Yang
AU  - Xicai Li
Y1  - 2016/12/05
PY  - 2016
N1  - https://doi.org/10.11648/j.abb.20160406.13
DO  - 10.11648/j.abb.20160406.13
T2  - Advances in Bioscience and Bioengineering
JF  - Advances in Bioscience and Bioengineering
JO  - Advances in Bioscience and Bioengineering
SP  - 79
EP  - 84
PB  - Science Publishing Group
SN  - 2330-4162
UR  - https://doi.org/10.11648/j.abb.20160406.13
AB  - A miniaturization time-resolved fluorescence analyzer was designed based on immunofluorescence and embedded technology, this instrument can be utilized to detect the immunochromatographic strip to achieve quantitative inspection and analysis of analytes in human blood or body fluid. By mechanical scanning means point-by-point assay, using UV-LED irradiation the fluorescence labeled immunocomplex on the immunochromatographic strip, and the fluorescence was emitted after being excited. Fluorescent light passes through narrow-band optical filters and converted to electrical signal by Silicon photodiode (Si PIN). After amplification and analog-digital conversion, the signal is sent to the micro-controller STM32F103 for processing. The fluorescence intensity distribution of the reagent strip was obtained after the scan detection finished, and the concentration of the substance can be calculated based on the known standard curve. After testing, the instrument has a wide dynamic range of measurement. It not only has a reliable repeatability (CV<0.2%) performance in the detection of concentration larger than 100µg/mL, but also hasdemonstrate stable validate features (CV=2.6%) when the concentration only at 1µg/mL. It has commendably application prospects in point-of-care test detection (POCT).
VL  - 4
IS  - 6
ER  -

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Author Information

Chongde Zi

Color & Image Vision Lab, Yunnan Normal Univ, Kunming, China
Junsheng Shi

Color & Image Vision Lab, Yunnan Normal Univ, Kunming, China
Yonghang Tai

Color & Image Vision Lab, Yunnan Normal Univ, Kunming, China
Huan Yang

Color & Image Vision Lab, Yunnan Normal Univ, Kunming, China
Xicai Li

Color & Image Vision Lab, Yunnan Normal Univ, Kunming, China

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Plain Text BibTeX RIS

APA Style

Chongde Zi, Junsheng Shi, Yonghang Tai, Huan Yang, Xicai Li. (2016). Design of Portable Time-Resolved Fluorometer. Advances in Bioscience and Bioengineering, 4(6), 79-84. https://doi.org/10.11648/j.abb.20160406.13

Copy | Download

ACS Style

Chongde Zi; Junsheng Shi; Yonghang Tai; Huan Yang; Xicai Li. Design of Portable Time-Resolved Fluorometer. Adv. BioSci. Bioeng. 2016, 4(6), 79-84. doi: 10.11648/j.abb.20160406.13

Copy | Download

AMA Style

Chongde Zi, Junsheng Shi, Yonghang Tai, Huan Yang, Xicai Li. Design of Portable Time-Resolved Fluorometer. Adv BioSci Bioeng. 2016;4(6):79-84. doi: 10.11648/j.abb.20160406.13

Copy | Download

@article{10.11648/j.abb.20160406.13,
  author = {Chongde Zi and Junsheng Shi and Yonghang Tai and Huan Yang and Xicai Li},
  title = {Design of Portable Time-Resolved Fluorometer},
  journal = {Advances in Bioscience and Bioengineering},
  volume = {4},
  number = {6},
  pages = {79-84},
  doi = {10.11648/j.abb.20160406.13},
  url = {https://doi.org/10.11648/j.abb.20160406.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.abb.20160406.13},
  abstract = {A miniaturization time-resolved fluorescence analyzer was designed based on immunofluorescence and embedded technology, this instrument can be utilized to detect the immunochromatographic strip to achieve quantitative inspection and analysis of analytes in human blood or body fluid. By mechanical scanning means point-by-point assay, using UV-LED irradiation the fluorescence labeled immunocomplex on the immunochromatographic strip, and the fluorescence was emitted after being excited. Fluorescent light passes through narrow-band optical filters and converted to electrical signal by Silicon photodiode (Si PIN). After amplification and analog-digital conversion, the signal is sent to the micro-controller STM32F103 for processing. The fluorescence intensity distribution of the reagent strip was obtained after the scan detection finished, and the concentration of the substance can be calculated based on the known standard curve. After testing, the instrument has a wide dynamic range of measurement. It not only has a reliable repeatability (CV<0.2%) performance in the detection of concentration larger than 100µg/mL, but also hasdemonstrate stable validate features (CV=2.6%) when the concentration only at 1µg/mL. It has commendably application prospects in point-of-care test detection (POCT).},
 year = {2016}
}

Copy | Download

TY  - JOUR
T1  - Design of Portable Time-Resolved Fluorometer
AU  - Chongde Zi
AU  - Junsheng Shi
AU  - Yonghang Tai
AU  - Huan Yang
AU  - Xicai Li
Y1  - 2016/12/05
PY  - 2016
N1  - https://doi.org/10.11648/j.abb.20160406.13
DO  - 10.11648/j.abb.20160406.13
T2  - Advances in Bioscience and Bioengineering
JF  - Advances in Bioscience and Bioengineering
JO  - Advances in Bioscience and Bioengineering
SP  - 79
EP  - 84
PB  - Science Publishing Group
SN  - 2330-4162
UR  - https://doi.org/10.11648/j.abb.20160406.13
AB  - A miniaturization time-resolved fluorescence analyzer was designed based on immunofluorescence and embedded technology, this instrument can be utilized to detect the immunochromatographic strip to achieve quantitative inspection and analysis of analytes in human blood or body fluid. By mechanical scanning means point-by-point assay, using UV-LED irradiation the fluorescence labeled immunocomplex on the immunochromatographic strip, and the fluorescence was emitted after being excited. Fluorescent light passes through narrow-band optical filters and converted to electrical signal by Silicon photodiode (Si PIN). After amplification and analog-digital conversion, the signal is sent to the micro-controller STM32F103 for processing. The fluorescence intensity distribution of the reagent strip was obtained after the scan detection finished, and the concentration of the substance can be calculated based on the known standard curve. After testing, the instrument has a wide dynamic range of measurement. It not only has a reliable repeatability (CV<0.2%) performance in the detection of concentration larger than 100µg/mL, but also hasdemonstrate stable validate features (CV=2.6%) when the concentration only at 1µg/mL. It has commendably application prospects in point-of-care test detection (POCT).
VL  - 4
IS  - 6
ER  -

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