Physiological and Symbiotic Characteristics of Rhizobia Isolated from <i>Medicago Polymorpha</i> L and Trigonella Stellate L Growing in semi-arid Regions of Libya

Salah Hassan Mohamed; Massoudah Omar Abulqasem Khalifa; Nazar Nasreldeen Babiker

doi:doi:10.11648/j.abb.20180604.11

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Physiological and Symbiotic Characteristics of Rhizobia Isolated from Medicago Polymorpha L and Trigonella Stellate L Growing in semi-arid Regions of Libya

Salah Hassan Mohamed, Massoudah Omar Abulqasem Khalifa, Nazar Nasreldeen Babiker

Published in Advances in Bioscience and Bioengineering (Volume 6, Issue 4)

Received: 14 January 2019 Accepted: 18 March 2019 Published: 9 April 2019

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Abstract

Physiological and symbiotic characteristics of 17 indigenous rhizobial isolates obtained from root nodules of wild legumes, (Trigonella stellate L. and Medicago polymorpha L.) that growing in different locations of Libya (northeast and northwest) and their cross-nodulation with cultivated Medicago sativa were studied. The results showed that the isolates were effective in their symbiosis with cultivated Medicago sativa L. A numerical taxonomic analysis performed on 56 non-symbiotic characteristics showed that at similarity level of 87%, the isolates formed four distinguished groups and three isolates remained separate. The results showed high variability among the isolates in their tolerance to various temperatures, and the majority of rhizobia isolates were sensitive to high acidity and are not able to grow at pH of 4.5. The tested isolates demonstrated a wide diversity in their tolerance to salinity, from sensitive unable to grow at 1% (w/v) to resistant to high salinity on a medium containing 4% (w/v) of NaCl. All isolates forming the four groups, including reference strains, were sensitive to CuCl₂.2H₂O, but they grew in medium containing HgCl₂. The isolates forming the four groups varied in their resistance to salts. The rhizobial isolates revealed a great diversity in their ability to react with antibiotic from sensitive to resistant.

Published in	Advances in Bioscience and Bioengineering (Volume 6, Issue 4)
DOI	10.11648/j.abb.20180604.11
Page(s)	34-41
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2019. Published by Science Publishing Group

Keywords

Libya, Trigonella Stellata, Medicago Polymorpha, Rhizobia, Physiological, Symbiotic, Characteristics

References

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Salah Hassan Mohamed, Massoudah Omar Abulqasem Khalifa, Nazar Nasreldeen Babiker. (2019). Physiological and Symbiotic Characteristics of Rhizobia Isolated from Medicago Polymorpha L and Trigonella Stellate L Growing in semi-arid Regions of Libya. Advances in Bioscience and Bioengineering, 6(4), 34-41. https://doi.org/10.11648/j.abb.20180604.11

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Salah Hassan Mohamed; Massoudah Omar Abulqasem Khalifa; Nazar Nasreldeen Babiker. Physiological and Symbiotic Characteristics of Rhizobia Isolated from Medicago Polymorpha L and Trigonella Stellate L Growing in semi-arid Regions of Libya. Adv. BioSci. Bioeng. 2019, 6(4), 34-41. doi: 10.11648/j.abb.20180604.11

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AMA Style

Salah Hassan Mohamed, Massoudah Omar Abulqasem Khalifa, Nazar Nasreldeen Babiker. Physiological and Symbiotic Characteristics of Rhizobia Isolated from Medicago Polymorpha L and Trigonella Stellate L Growing in semi-arid Regions of Libya. Adv BioSci Bioeng. 2019;6(4):34-41. doi: 10.11648/j.abb.20180604.11

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@article{10.11648/j.abb.20180604.11,
  author = {Salah Hassan Mohamed and Massoudah Omar Abulqasem Khalifa and Nazar Nasreldeen Babiker},
  title = {Physiological and Symbiotic Characteristics of Rhizobia Isolated from Medicago Polymorpha L and Trigonella Stellate L Growing in semi-arid Regions of Libya},
  journal = {Advances in Bioscience and Bioengineering},
  volume = {6},
  number = {4},
  pages = {34-41},
  doi = {10.11648/j.abb.20180604.11},
  url = {https://doi.org/10.11648/j.abb.20180604.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.abb.20180604.11},
  abstract = {Physiological and symbiotic characteristics of 17 indigenous rhizobial isolates obtained from root nodules of wild legumes, (Trigonella stellate L. and Medicago polymorpha L.) that growing in different locations of Libya (northeast and northwest) and their cross-nodulation with cultivated Medicago sativa were studied. The results showed that the isolates were effective in their symbiosis with cultivated Medicago sativa L. A numerical taxonomic analysis performed on 56 non-symbiotic characteristics showed that at similarity level of 87%, the isolates formed four distinguished groups and three isolates remained separate. The results showed high variability among the isolates in their tolerance to various temperatures, and the majority of rhizobia isolates were sensitive to high acidity and are not able to grow at pH of 4.5. The tested isolates demonstrated a wide diversity in their tolerance to salinity, from sensitive unable to grow at 1% (w/v) to resistant to high salinity on a medium containing 4% (w/v) of NaCl. All isolates forming the four groups, including reference strains, were sensitive to CuCl2.2H2O, but they grew in medium containing HgCl2. The isolates forming the four groups varied in their resistance to salts. The rhizobial isolates revealed a great diversity in their ability to react with antibiotic from sensitive to resistant.},
 year = {2019}
}

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TY  - JOUR
T1  - Physiological and Symbiotic Characteristics of Rhizobia Isolated from Medicago Polymorpha L and Trigonella Stellate L Growing in semi-arid Regions of Libya
AU  - Salah Hassan Mohamed
AU  - Massoudah Omar Abulqasem Khalifa
AU  - Nazar Nasreldeen Babiker
Y1  - 2019/04/09
PY  - 2019
N1  - https://doi.org/10.11648/j.abb.20180604.11
DO  - 10.11648/j.abb.20180604.11
T2  - Advances in Bioscience and Bioengineering
JF  - Advances in Bioscience and Bioengineering
JO  - Advances in Bioscience and Bioengineering
SP  - 34
EP  - 41
PB  - Science Publishing Group
SN  - 2330-4162
UR  - https://doi.org/10.11648/j.abb.20180604.11
AB  - Physiological and symbiotic characteristics of 17 indigenous rhizobial isolates obtained from root nodules of wild legumes, (Trigonella stellate L. and Medicago polymorpha L.) that growing in different locations of Libya (northeast and northwest) and their cross-nodulation with cultivated Medicago sativa were studied. The results showed that the isolates were effective in their symbiosis with cultivated Medicago sativa L. A numerical taxonomic analysis performed on 56 non-symbiotic characteristics showed that at similarity level of 87%, the isolates formed four distinguished groups and three isolates remained separate. The results showed high variability among the isolates in their tolerance to various temperatures, and the majority of rhizobia isolates were sensitive to high acidity and are not able to grow at pH of 4.5. The tested isolates demonstrated a wide diversity in their tolerance to salinity, from sensitive unable to grow at 1% (w/v) to resistant to high salinity on a medium containing 4% (w/v) of NaCl. All isolates forming the four groups, including reference strains, were sensitive to CuCl2.2H2O, but they grew in medium containing HgCl2. The isolates forming the four groups varied in their resistance to salts. The rhizobial isolates revealed a great diversity in their ability to react with antibiotic from sensitive to resistant.
VL  - 6
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Author Information

Salah Hassan Mohamed

Department of Botany, Faculty of Sciences, Sebha University, Sebha, Libya
Massoudah Omar Abulqasem Khalifa

Department of Botany, Faculty of Sciences, Sebha University, Sebha, Libya
Nazar Nasreldeen Babiker

Department of Soil and Water Science, Faculty of Agricultural sciences, University of Gezira, Wad Medani, sudan

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Salah Hassan Mohamed, Massoudah Omar Abulqasem Khalifa, Nazar Nasreldeen Babiker. (2019). Physiological and Symbiotic Characteristics of Rhizobia Isolated from Medicago Polymorpha L and Trigonella Stellate L Growing in semi-arid Regions of Libya. Advances in Bioscience and Bioengineering, 6(4), 34-41. https://doi.org/10.11648/j.abb.20180604.11

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Salah Hassan Mohamed; Massoudah Omar Abulqasem Khalifa; Nazar Nasreldeen Babiker. Physiological and Symbiotic Characteristics of Rhizobia Isolated from Medicago Polymorpha L and Trigonella Stellate L Growing in semi-arid Regions of Libya. Adv. BioSci. Bioeng. 2019, 6(4), 34-41. doi: 10.11648/j.abb.20180604.11

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AMA Style

Salah Hassan Mohamed, Massoudah Omar Abulqasem Khalifa, Nazar Nasreldeen Babiker. Physiological and Symbiotic Characteristics of Rhizobia Isolated from Medicago Polymorpha L and Trigonella Stellate L Growing in semi-arid Regions of Libya. Adv BioSci Bioeng. 2019;6(4):34-41. doi: 10.11648/j.abb.20180604.11

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@article{10.11648/j.abb.20180604.11,
  author = {Salah Hassan Mohamed and Massoudah Omar Abulqasem Khalifa and Nazar Nasreldeen Babiker},
  title = {Physiological and Symbiotic Characteristics of Rhizobia Isolated from Medicago Polymorpha L and Trigonella Stellate L Growing in semi-arid Regions of Libya},
  journal = {Advances in Bioscience and Bioengineering},
  volume = {6},
  number = {4},
  pages = {34-41},
  doi = {10.11648/j.abb.20180604.11},
  url = {https://doi.org/10.11648/j.abb.20180604.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.abb.20180604.11},
  abstract = {Physiological and symbiotic characteristics of 17 indigenous rhizobial isolates obtained from root nodules of wild legumes, (Trigonella stellate L. and Medicago polymorpha L.) that growing in different locations of Libya (northeast and northwest) and their cross-nodulation with cultivated Medicago sativa were studied. The results showed that the isolates were effective in their symbiosis with cultivated Medicago sativa L. A numerical taxonomic analysis performed on 56 non-symbiotic characteristics showed that at similarity level of 87%, the isolates formed four distinguished groups and three isolates remained separate. The results showed high variability among the isolates in their tolerance to various temperatures, and the majority of rhizobia isolates were sensitive to high acidity and are not able to grow at pH of 4.5. The tested isolates demonstrated a wide diversity in their tolerance to salinity, from sensitive unable to grow at 1% (w/v) to resistant to high salinity on a medium containing 4% (w/v) of NaCl. All isolates forming the four groups, including reference strains, were sensitive to CuCl2.2H2O, but they grew in medium containing HgCl2. The isolates forming the four groups varied in their resistance to salts. The rhizobial isolates revealed a great diversity in their ability to react with antibiotic from sensitive to resistant.},
 year = {2019}
}

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TY  - JOUR
T1  - Physiological and Symbiotic Characteristics of Rhizobia Isolated from Medicago Polymorpha L and Trigonella Stellate L Growing in semi-arid Regions of Libya
AU  - Salah Hassan Mohamed
AU  - Massoudah Omar Abulqasem Khalifa
AU  - Nazar Nasreldeen Babiker
Y1  - 2019/04/09
PY  - 2019
N1  - https://doi.org/10.11648/j.abb.20180604.11
DO  - 10.11648/j.abb.20180604.11
T2  - Advances in Bioscience and Bioengineering
JF  - Advances in Bioscience and Bioengineering
JO  - Advances in Bioscience and Bioengineering
SP  - 34
EP  - 41
PB  - Science Publishing Group
SN  - 2330-4162
UR  - https://doi.org/10.11648/j.abb.20180604.11
AB  - Physiological and symbiotic characteristics of 17 indigenous rhizobial isolates obtained from root nodules of wild legumes, (Trigonella stellate L. and Medicago polymorpha L.) that growing in different locations of Libya (northeast and northwest) and their cross-nodulation with cultivated Medicago sativa were studied. The results showed that the isolates were effective in their symbiosis with cultivated Medicago sativa L. A numerical taxonomic analysis performed on 56 non-symbiotic characteristics showed that at similarity level of 87%, the isolates formed four distinguished groups and three isolates remained separate. The results showed high variability among the isolates in their tolerance to various temperatures, and the majority of rhizobia isolates were sensitive to high acidity and are not able to grow at pH of 4.5. The tested isolates demonstrated a wide diversity in their tolerance to salinity, from sensitive unable to grow at 1% (w/v) to resistant to high salinity on a medium containing 4% (w/v) of NaCl. All isolates forming the four groups, including reference strains, were sensitive to CuCl2.2H2O, but they grew in medium containing HgCl2. The isolates forming the four groups varied in their resistance to salts. The rhizobial isolates revealed a great diversity in their ability to react with antibiotic from sensitive to resistant.
VL  - 6
IS  - 4
ER  -

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