Optimization of Amylase Production from <i>Lactobacillus plantarum</i> CS by Submerged Fermentation Using Agro Wastes Substrates

George-Okafor Uzoamaka Ogechi; Nwachukwu Ujunwa Felicia; Ezeme-Nwafor Amara

doi:doi:10.11648/j.abb.20221001.12

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Optimization of Amylase Production from Lactobacillus plantarum CS by Submerged Fermentation Using Agro Wastes Substrates

George-Okafor Uzoamaka Ogechi, Nwachukwu Ujunwa Felicia, Ezeme-Nwafor Amara

Published in Advances in Bioscience and Bioengineering (Volume 10, Issue 1)

Received: 5 January 2022 Accepted: 24 January 2022 Published: 9 February 2022

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Abstract

Amylases are known to be of great importance worldwide. Most of their production using refined carbon and nitrogen substrates are expensive, resulting in the production of expensive goods which are not easily affordable by end users in our country Nigeria. This necessitates the call for the use of cost-effective substrates under optimized condition for maximum amylase yield. Hence, the present study was focused on the optimization of amylase production from Lactobacillus plantarum CS using agro wastes from local substrates. The parameters assayed were carbon and nitrogen substrates from agro wastes, fermentation period (24-168h), pH (3.5-11) and temperature (30°C-70°C). Others included solvents for preparation of fermentation medium and metal ions of Mg²⁺, Mn²⁺, K⁺, Na⁺, Hg²⁺, Fe²⁺, Cu²⁺ and Pb²⁺ as supplements. The amylase produced via shake-flask fermentation was assayed using Dinitro salicylic acid method. The result revealed 3% sweet potato and 1% bambara nut wastes as the best carbon and nitrogen components of the required fermentation medium of pH 6.5. The optimal amylase produced (38.6U/ml) was achieved within 48h at 35°C with 1% inoculum and Mn²⁺/ Mg²⁺ as a co-factor. Relatively, Cu²⁺, Pb²⁺ and Hg²⁺ reduced the amylase yield to < 68.13% as against Mn²⁺ and Mg²⁺ which enhanced the production to 121.66% and 101.09% respectively. Natural rain water used as solvent for the preparation of fermentation medium significantly enhanced amylase production (24.65 U/ml) in relation to other test waters at P>0.05. Comparatively, there was a significant increase in amylase produced under optimized condition than not. Conclusively, the obtained results which revealed high amylase yield with available and cost effective agro-wastes is promising for industrial application in Nigeria.

Published in	Advances in Bioscience and Bioengineering (Volume 10, Issue 1)
DOI	10.11648/j.abb.20221001.12
Page(s)	11-18
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2022. Published by Science Publishing Group

Keywords

Amylase, L. plantarum CS, Optimization, Agro Wastes, Fermentation

References

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George-Okafor Uzoamaka Ogechi, Nwachukwu Ujunwa Felicia, Ezeme-Nwafor Amara. (2022). Optimization of Amylase Production from Lactobacillus plantarum CS by Submerged Fermentation Using Agro Wastes Substrates. Advances in Bioscience and Bioengineering, 10(1), 11-18. https://doi.org/10.11648/j.abb.20221001.12

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George-Okafor Uzoamaka Ogechi; Nwachukwu Ujunwa Felicia; Ezeme-Nwafor Amara. Optimization of Amylase Production from Lactobacillus plantarum CS by Submerged Fermentation Using Agro Wastes Substrates. Adv. BioSci. Bioeng. 2022, 10(1), 11-18. doi: 10.11648/j.abb.20221001.12

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AMA Style

George-Okafor Uzoamaka Ogechi, Nwachukwu Ujunwa Felicia, Ezeme-Nwafor Amara. Optimization of Amylase Production from Lactobacillus plantarum CS by Submerged Fermentation Using Agro Wastes Substrates. Adv BioSci Bioeng. 2022;10(1):11-18. doi: 10.11648/j.abb.20221001.12

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@article{10.11648/j.abb.20221001.12,
  author = {George-Okafor Uzoamaka Ogechi and Nwachukwu Ujunwa Felicia and Ezeme-Nwafor Amara},
  title = {Optimization of Amylase Production from Lactobacillus plantarum CS by Submerged Fermentation Using Agro Wastes Substrates},
  journal = {Advances in Bioscience and Bioengineering},
  volume = {10},
  number = {1},
  pages = {11-18},
  doi = {10.11648/j.abb.20221001.12},
  url = {https://doi.org/10.11648/j.abb.20221001.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.abb.20221001.12},
  abstract = {Amylases are known to be of great importance worldwide. Most of their production using refined carbon and nitrogen substrates are expensive, resulting in the production of expensive goods which are not easily affordable by end users in our country Nigeria. This necessitates the call for the use of cost-effective substrates under optimized condition for maximum amylase yield. Hence, the present study was focused on the optimization of amylase production from Lactobacillus plantarum CS using agro wastes from local substrates. The parameters assayed were carbon and nitrogen substrates from agro wastes, fermentation period (24-168h), pH (3.5-11) and temperature (30°C-70°C). Others included solvents for preparation of fermentation medium and metal ions of Mg2+, Mn2+, K+, Na+, Hg2+, Fe2+, Cu2+ and Pb2+ as supplements. The amylase produced via shake-flask fermentation was assayed using Dinitro salicylic acid method. The result revealed 3% sweet potato and 1% bambara nut wastes as the best carbon and nitrogen components of the required fermentation medium of pH 6.5. The optimal amylase produced (38.6U/ml) was achieved within 48h at 35°C with 1% inoculum and Mn2+/ Mg2+ as a co-factor. Relatively, Cu2+, Pb2+ and Hg2+ reduced the amylase yield to 2+ and Mg2+ which enhanced the production to 121.66% and 101.09% respectively. Natural rain water used as solvent for the preparation of fermentation medium significantly enhanced amylase production (24.65 U/ml) in relation to other test waters at P>0.05. Comparatively, there was a significant increase in amylase produced under optimized condition than not. Conclusively, the obtained results which revealed high amylase yield with available and cost effective agro-wastes is promising for industrial application in Nigeria.},
 year = {2022}
}

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TY - JOUR
T1 - Optimization of Amylase Production from Lactobacillus plantarum CS by Submerged Fermentation Using Agro Wastes Substrates
AU - George-Okafor Uzoamaka Ogechi
AU - Nwachukwu Ujunwa Felicia
AU - Ezeme-Nwafor Amara
Y1 - 2022/02/09
PY - 2022
N1 - https://doi.org/10.11648/j.abb.20221001.12
DO - 10.11648/j.abb.20221001.12
T2 - Advances in Bioscience and Bioengineering
JF - Advances in Bioscience and Bioengineering
JO - Advances in Bioscience and Bioengineering
SP - 11
EP - 18
PB - Science Publishing Group
SN - 2330-4162
UR - https://doi.org/10.11648/j.abb.20221001.12
AB - Amylases are known to be of great importance worldwide. Most of their production using refined carbon and nitrogen substrates are expensive, resulting in the production of expensive goods which are not easily affordable by end users in our country Nigeria. This necessitates the call for the use of cost-effective substrates under optimized condition for maximum amylase yield. Hence, the present study was focused on the optimization of amylase production from Lactobacillus plantarum CS using agro wastes from local substrates. The parameters assayed were carbon and nitrogen substrates from agro wastes, fermentation period (24-168h), pH (3.5-11) and temperature (30°C-70°C). Others included solvents for preparation of fermentation medium and metal ions of Mg2+, Mn2+, K+, Na+, Hg2+, Fe2+, Cu2+ and Pb2+ as supplements. The amylase produced via shake-flask fermentation was assayed using Dinitro salicylic acid method. The result revealed 3% sweet potato and 1% bambara nut wastes as the best carbon and nitrogen components of the required fermentation medium of pH 6.5. The optimal amylase produced (38.6U/ml) was achieved within 48h at 35°C with 1% inoculum and Mn2+/ Mg2+ as a co-factor. Relatively, Cu2+, Pb2+ and Hg2+ reduced the amylase yield to 2+ and Mg2+ which enhanced the production to 121.66% and 101.09% respectively. Natural rain water used as solvent for the preparation of fermentation medium significantly enhanced amylase production (24.65 U/ml) in relation to other test waters at P>0.05. Comparatively, there was a significant increase in amylase produced under optimized condition than not. Conclusively, the obtained results which revealed high amylase yield with available and cost effective agro-wastes is promising for industrial application in Nigeria.
VL - 10
IS - 1
ER -

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George-Okafor Uzoamaka Ogechi

Department of Applied Microbiology and Brewing, Faculty of Applied Natural Sciences, Enugu State University of Science and Technology, Agbani, Nigeria
Nwachukwu Ujunwa Felicia

Department of Applied Microbiology and Brewing, Faculty of Applied Natural Sciences, Enugu State University of Science and Technology, Agbani, Nigeria
Ezeme-Nwafor Amara

Department of Applied Microbiology and Brewing, Faculty of Applied Natural Sciences, Enugu State University of Science and Technology, Agbani, Nigeria

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George-Okafor Uzoamaka Ogechi, Nwachukwu Ujunwa Felicia, Ezeme-Nwafor Amara. (2022). Optimization of Amylase Production from Lactobacillus plantarum CS by Submerged Fermentation Using Agro Wastes Substrates. Advances in Bioscience and Bioengineering, 10(1), 11-18. https://doi.org/10.11648/j.abb.20221001.12

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George-Okafor Uzoamaka Ogechi; Nwachukwu Ujunwa Felicia; Ezeme-Nwafor Amara. Optimization of Amylase Production from Lactobacillus plantarum CS by Submerged Fermentation Using Agro Wastes Substrates. Adv. BioSci. Bioeng. 2022, 10(1), 11-18. doi: 10.11648/j.abb.20221001.12

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AMA Style

George-Okafor Uzoamaka Ogechi, Nwachukwu Ujunwa Felicia, Ezeme-Nwafor Amara. Optimization of Amylase Production from Lactobacillus plantarum CS by Submerged Fermentation Using Agro Wastes Substrates. Adv BioSci Bioeng. 2022;10(1):11-18. doi: 10.11648/j.abb.20221001.12

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@article{10.11648/j.abb.20221001.12,
  author = {George-Okafor Uzoamaka Ogechi and Nwachukwu Ujunwa Felicia and Ezeme-Nwafor Amara},
  title = {Optimization of Amylase Production from Lactobacillus plantarum CS by Submerged Fermentation Using Agro Wastes Substrates},
  journal = {Advances in Bioscience and Bioengineering},
  volume = {10},
  number = {1},
  pages = {11-18},
  doi = {10.11648/j.abb.20221001.12},
  url = {https://doi.org/10.11648/j.abb.20221001.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.abb.20221001.12},
  abstract = {Amylases are known to be of great importance worldwide. Most of their production using refined carbon and nitrogen substrates are expensive, resulting in the production of expensive goods which are not easily affordable by end users in our country Nigeria. This necessitates the call for the use of cost-effective substrates under optimized condition for maximum amylase yield. Hence, the present study was focused on the optimization of amylase production from Lactobacillus plantarum CS using agro wastes from local substrates. The parameters assayed were carbon and nitrogen substrates from agro wastes, fermentation period (24-168h), pH (3.5-11) and temperature (30°C-70°C). Others included solvents for preparation of fermentation medium and metal ions of Mg2+, Mn2+, K+, Na+, Hg2+, Fe2+, Cu2+ and Pb2+ as supplements. The amylase produced via shake-flask fermentation was assayed using Dinitro salicylic acid method. The result revealed 3% sweet potato and 1% bambara nut wastes as the best carbon and nitrogen components of the required fermentation medium of pH 6.5. The optimal amylase produced (38.6U/ml) was achieved within 48h at 35°C with 1% inoculum and Mn2+/ Mg2+ as a co-factor. Relatively, Cu2+, Pb2+ and Hg2+ reduced the amylase yield to 2+ and Mg2+ which enhanced the production to 121.66% and 101.09% respectively. Natural rain water used as solvent for the preparation of fermentation medium significantly enhanced amylase production (24.65 U/ml) in relation to other test waters at P>0.05. Comparatively, there was a significant increase in amylase produced under optimized condition than not. Conclusively, the obtained results which revealed high amylase yield with available and cost effective agro-wastes is promising for industrial application in Nigeria.},
 year = {2022}
}

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