The Effects of Chilling on Antioxidant Enzyme System and Related Gene Expression Levels in Sweet Corn Seeds with Different Germination Characteristics

Tingzhen Wang; Zhenxing Wu; Jianjian Chen; Fangjian Li; Guihua Lv

doi:doi:10.11648/j.aff.20241306.11

Research Article |

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The Effects of Chilling on Antioxidant Enzyme System and Related Gene Expression Levels in Sweet Corn Seeds with Different Germination Characteristics

Tingzhen Wang

, Zhenxing Wu, Jianjian Chen, Fangjian Li, Guihua Lv^*

Published in Agriculture, Forestry and Fisheries (Volume 13, Issue 6)

Received: 8 October 2024 Accepted: 11 November 2024 Published: 21 November 2024

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Abstract

Sweet corn is a vegetable and grain dual-use crop with high economic value and industrial advantages. Low temperature stress significantly reduces the germination rate of sweet corn seeds, which has a negative impact on both quality and yield. This study used the chilling sensitive sweet corn inbred line 20hi111 and the chilling tolerant sweet corn inbred line T135 as experimental materials to measure the MDA (malondialdehyde) and H₂O₂content, CAT, POD, and SOD enzyme activities, and enzyme gene expression patterns during seed germination under low temperature (10°C) and normal temperature (25°C) treatments. The research results indicated that during low-temperature germination, the H₂O₂ content and CAT activity of 20hi111 were generally higher than those of T135, while the POD and SOD activities and MDA content were generally lower than those of T135. There was no strict consistency between gene expression and enzyme activity. At low temperature, the expression of ZmCAT1 and ZmCAT3 in 20hi111 was significantly higher than T135, while there was no significant difference in ZmPOD1. In 20hi111, ZmPOD3 was first lower and then higher than T135, and ZmSOD3 and ZmSOD9 were lower than T135 under low temperature treatment. In this study, the activity of antioxidant enzymes and the expression of antioxidant enzyme-related genes in sweet corn inbred lines with different germination characteristics under low temperature were analyzed, which provided some theoretical basis for cultivating sweet corn varieties with low temperature tolerance.

Published in	Agriculture, Forestry and Fisheries (Volume 13, Issue 6)
DOI	10.11648/j.aff.20241306.11
Page(s)	224-234
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Sweet Corn, Germination, Low Temperature, Antioxidant Enzyme Activity

References

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APA Style

Wang, T., Wu, Z., Chen, J., Li, F., Lv, G. (2024). The Effects of Chilling on Antioxidant Enzyme System and Related Gene Expression Levels in Sweet Corn Seeds with Different Germination Characteristics. Agriculture, Forestry and Fisheries, 13(6), 224-234. https://doi.org/10.11648/j.aff.20241306.11

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ACS Style

Wang, T.; Wu, Z.; Chen, J.; Li, F.; Lv, G. The Effects of Chilling on Antioxidant Enzyme System and Related Gene Expression Levels in Sweet Corn Seeds with Different Germination Characteristics. Agric. For. Fish. 2024, 13(6), 224-234. doi: 10.11648/j.aff.20241306.11

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AMA Style

Wang T, Wu Z, Chen J, Li F, Lv G. The Effects of Chilling on Antioxidant Enzyme System and Related Gene Expression Levels in Sweet Corn Seeds with Different Germination Characteristics. Agric For Fish. 2024;13(6):224-234. doi: 10.11648/j.aff.20241306.11

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@article{10.11648/j.aff.20241306.11,
  author = {Tingzhen Wang and Zhenxing Wu and Jianjian Chen and Fangjian Li and Guihua Lv},
  title = {The Effects of Chilling on Antioxidant Enzyme System and Related Gene Expression Levels in Sweet Corn Seeds with Different Germination Characteristics
},
  journal = {Agriculture, Forestry and Fisheries},
  volume = {13},
  number = {6},
  pages = {224-234},
  doi = {10.11648/j.aff.20241306.11},
  url = {https://doi.org/10.11648/j.aff.20241306.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.aff.20241306.11},
  abstract = {Sweet corn is a vegetable and grain dual-use crop with high economic value and industrial advantages. Low temperature stress significantly reduces the germination rate of sweet corn seeds, which has a negative impact on both quality and yield. This study used the chilling sensitive sweet corn inbred line 20hi111 and the chilling tolerant sweet corn inbred line T135 as experimental materials to measure the MDA (malondialdehyde) and H2O2 content, CAT, POD, and SOD enzyme activities, and enzyme gene expression patterns during seed germination under low temperature (10°C) and normal temperature (25°C) treatments. The research results indicated that during low-temperature germination, the H2O2 content and CAT activity of 20hi111 were generally higher than those of T135, while the POD and SOD activities and MDA content were generally lower than those of T135. There was no strict consistency between gene expression and enzyme activity. At low temperature, the expression of ZmCAT1 and ZmCAT3 in 20hi111 was significantly higher than T135, while there was no significant difference in ZmPOD1. In 20hi111, ZmPOD3 was first lower and then higher than T135, and ZmSOD3 and ZmSOD9 were lower than T135 under low temperature treatment. In this study, the activity of antioxidant enzymes and the expression of antioxidant enzyme-related genes in sweet corn inbred lines with different germination characteristics under low temperature were analyzed, which provided some theoretical basis for cultivating sweet corn varieties with low temperature tolerance.
 },
 year = {2024}
}

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TY  - JOUR
T1  - The Effects of Chilling on Antioxidant Enzyme System and Related Gene Expression Levels in Sweet Corn Seeds with Different Germination Characteristics

AU  - Tingzhen Wang
AU  - Zhenxing Wu
AU  - Jianjian Chen
AU  - Fangjian Li
AU  - Guihua Lv
Y1  - 2024/11/21
PY  - 2024
N1  - https://doi.org/10.11648/j.aff.20241306.11
DO  - 10.11648/j.aff.20241306.11
T2  - Agriculture, Forestry and Fisheries
JF  - Agriculture, Forestry and Fisheries
JO  - Agriculture, Forestry and Fisheries
SP  - 224
EP  - 234
PB  - Science Publishing Group
SN  - 2328-5648
UR  - https://doi.org/10.11648/j.aff.20241306.11
AB  - Sweet corn is a vegetable and grain dual-use crop with high economic value and industrial advantages. Low temperature stress significantly reduces the germination rate of sweet corn seeds, which has a negative impact on both quality and yield. This study used the chilling sensitive sweet corn inbred line 20hi111 and the chilling tolerant sweet corn inbred line T135 as experimental materials to measure the MDA (malondialdehyde) and H2O2 content, CAT, POD, and SOD enzyme activities, and enzyme gene expression patterns during seed germination under low temperature (10°C) and normal temperature (25°C) treatments. The research results indicated that during low-temperature germination, the H2O2 content and CAT activity of 20hi111 were generally higher than those of T135, while the POD and SOD activities and MDA content were generally lower than those of T135. There was no strict consistency between gene expression and enzyme activity. At low temperature, the expression of ZmCAT1 and ZmCAT3 in 20hi111 was significantly higher than T135, while there was no significant difference in ZmPOD1. In 20hi111, ZmPOD3 was first lower and then higher than T135, and ZmSOD3 and ZmSOD9 were lower than T135 under low temperature treatment. In this study, the activity of antioxidant enzymes and the expression of antioxidant enzyme-related genes in sweet corn inbred lines with different germination characteristics under low temperature were analyzed, which provided some theoretical basis for cultivating sweet corn varieties with low temperature tolerance.
 
VL  - 13
IS  - 6
ER  -

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Author Information

Tingzhen Wang

Institute of Maize and Featured Upland Crops, Zhejiang Academy of Agricultural Sciences, Dongyang, China

http://orcid.org/0000-0003-1154-054X
Zhenxing Wu

Institute of Maize and Featured Upland Crops, Zhejiang Academy of Agricultural Sciences, Dongyang, China
Jianjian Chen

Institute of Maize and Featured Upland Crops, Zhejiang Academy of Agricultural Sciences, Dongyang, China
Fangjian Li

Institute of Maize and Featured Upland Crops, Zhejiang Academy of Agricultural Sciences, Dongyang, China
Guihua Lv

Institute of Maize and Featured Upland Crops, Zhejiang Academy of Agricultural Sciences, Dongyang, China

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APA Style

Wang, T., Wu, Z., Chen, J., Li, F., Lv, G. (2024). The Effects of Chilling on Antioxidant Enzyme System and Related Gene Expression Levels in Sweet Corn Seeds with Different Germination Characteristics. Agriculture, Forestry and Fisheries, 13(6), 224-234. https://doi.org/10.11648/j.aff.20241306.11

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ACS Style

Wang, T.; Wu, Z.; Chen, J.; Li, F.; Lv, G. The Effects of Chilling on Antioxidant Enzyme System and Related Gene Expression Levels in Sweet Corn Seeds with Different Germination Characteristics. Agric. For. Fish. 2024, 13(6), 224-234. doi: 10.11648/j.aff.20241306.11

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AMA Style

Wang T, Wu Z, Chen J, Li F, Lv G. The Effects of Chilling on Antioxidant Enzyme System and Related Gene Expression Levels in Sweet Corn Seeds with Different Germination Characteristics. Agric For Fish. 2024;13(6):224-234. doi: 10.11648/j.aff.20241306.11

Copy | Download

@article{10.11648/j.aff.20241306.11,
  author = {Tingzhen Wang and Zhenxing Wu and Jianjian Chen and Fangjian Li and Guihua Lv},
  title = {The Effects of Chilling on Antioxidant Enzyme System and Related Gene Expression Levels in Sweet Corn Seeds with Different Germination Characteristics
},
  journal = {Agriculture, Forestry and Fisheries},
  volume = {13},
  number = {6},
  pages = {224-234},
  doi = {10.11648/j.aff.20241306.11},
  url = {https://doi.org/10.11648/j.aff.20241306.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.aff.20241306.11},
  abstract = {Sweet corn is a vegetable and grain dual-use crop with high economic value and industrial advantages. Low temperature stress significantly reduces the germination rate of sweet corn seeds, which has a negative impact on both quality and yield. This study used the chilling sensitive sweet corn inbred line 20hi111 and the chilling tolerant sweet corn inbred line T135 as experimental materials to measure the MDA (malondialdehyde) and H2O2 content, CAT, POD, and SOD enzyme activities, and enzyme gene expression patterns during seed germination under low temperature (10°C) and normal temperature (25°C) treatments. The research results indicated that during low-temperature germination, the H2O2 content and CAT activity of 20hi111 were generally higher than those of T135, while the POD and SOD activities and MDA content were generally lower than those of T135. There was no strict consistency between gene expression and enzyme activity. At low temperature, the expression of ZmCAT1 and ZmCAT3 in 20hi111 was significantly higher than T135, while there was no significant difference in ZmPOD1. In 20hi111, ZmPOD3 was first lower and then higher than T135, and ZmSOD3 and ZmSOD9 were lower than T135 under low temperature treatment. In this study, the activity of antioxidant enzymes and the expression of antioxidant enzyme-related genes in sweet corn inbred lines with different germination characteristics under low temperature were analyzed, which provided some theoretical basis for cultivating sweet corn varieties with low temperature tolerance.
 },
 year = {2024}
}

Copy | Download

TY  - JOUR
T1  - The Effects of Chilling on Antioxidant Enzyme System and Related Gene Expression Levels in Sweet Corn Seeds with Different Germination Characteristics

AU  - Tingzhen Wang
AU  - Zhenxing Wu
AU  - Jianjian Chen
AU  - Fangjian Li
AU  - Guihua Lv
Y1  - 2024/11/21
PY  - 2024
N1  - https://doi.org/10.11648/j.aff.20241306.11
DO  - 10.11648/j.aff.20241306.11
T2  - Agriculture, Forestry and Fisheries
JF  - Agriculture, Forestry and Fisheries
JO  - Agriculture, Forestry and Fisheries
SP  - 224
EP  - 234
PB  - Science Publishing Group
SN  - 2328-5648
UR  - https://doi.org/10.11648/j.aff.20241306.11
AB  - Sweet corn is a vegetable and grain dual-use crop with high economic value and industrial advantages. Low temperature stress significantly reduces the germination rate of sweet corn seeds, which has a negative impact on both quality and yield. This study used the chilling sensitive sweet corn inbred line 20hi111 and the chilling tolerant sweet corn inbred line T135 as experimental materials to measure the MDA (malondialdehyde) and H2O2 content, CAT, POD, and SOD enzyme activities, and enzyme gene expression patterns during seed germination under low temperature (10°C) and normal temperature (25°C) treatments. The research results indicated that during low-temperature germination, the H2O2 content and CAT activity of 20hi111 were generally higher than those of T135, while the POD and SOD activities and MDA content were generally lower than those of T135. There was no strict consistency between gene expression and enzyme activity. At low temperature, the expression of ZmCAT1 and ZmCAT3 in 20hi111 was significantly higher than T135, while there was no significant difference in ZmPOD1. In 20hi111, ZmPOD3 was first lower and then higher than T135, and ZmSOD3 and ZmSOD9 were lower than T135 under low temperature treatment. In this study, the activity of antioxidant enzymes and the expression of antioxidant enzyme-related genes in sweet corn inbred lines with different germination characteristics under low temperature were analyzed, which provided some theoretical basis for cultivating sweet corn varieties with low temperature tolerance.
 
VL  - 13
IS  - 6
ER  -

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