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Production and Comprehensive Characterization of Banana Fruit Wine Using Saccharomyces Cerevisiae

Received: 12 November 2025     Accepted: 21 November 2025     Published: 20 December 2025
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Abstract

Bananas (Musa spp.) are a globally cultivated fruit valued for their flavor and rich nutritional content (high in sugars, potassium and B-vitamins). Because ripe bananas are highly perishable, converting excess or overripe bananas into wine can reduce waste and add value. Fruit wines are produced by fermenting fruit juices with yeast, turning sugars into ethanol, carbon dioxide and minor by-products that give wine its aroma and preserve it. In particular, the high sugar/carbohydrate content of banana makes it an excellent substrate for fermentation. Thus banana wine production has emerged as an attractive alternative to grape wine in regions where grapes are scarce: it harnesses surplus fruit, yields a vitamin-rich beverage, and extends shelf life through fermentation. In this study, ripe banana pulp was cleaned, mashed and blended with water, sugar and lemon juice, then inoculated with baker’s yeast (Saccharomyces cerevisiae) and fermented anaerobically for about six days. Fermentation progress was monitored by measuring pH, titratable acidity, specific gravity, total dissolved solids (TDS) and ethanol content at regular intervals. These analytical assays are standard for fruit wine characterization. The yeast consumes banana sugars to produce ethanol and CO2; as fermentation proceeded we observed vigorous bubbling (CO2 release) and viscosity reduction, as expected for Saccharomyces fermentation. The fermenting banana must showed the typical trends of wine fermentation. The pH fell markedly (from about 5.0 initially to ~2.0 by the end), reflecting increased organic acid production, while titratable acidity rose to ~1.6 g/100 mL (as lactic/acetic acid equivalents). Specific gravity declined (from ~0.983 to ~0.982), indicating sugar depletion and ethanol formation. Correspondingly, ethanol content increased steadily and reached about 14% v/v by day six. Observed ethanol rising to ~15% as pH dropped into the low-3 range during fruit fermentation. A clear, amber banana wine was obtained after racking; it exhibited the characteristic aroma and flavor of banana and acceptable clarity.

Published in American Journal of Applied and Industrial Chemistry (Volume 9, Issue 2)
DOI 10.11648/j.ajaic.20250902.15
Page(s) 74-79
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2025. Published by Science Publishing Group

Keywords

Banana Wine, Fermentation, Saccharomyces Cerevisiae, Fruit Wine, Ethanol Content, Titratable Acidity

References
[1] Awe, S., Eniola, K. I. T. and Kayode-Ishola, T. M., 2013. Proximate and mineral composition of locally produced pawpaw and banana wine. American Journal of Research Communication, 1(12), pp. 390-397.
[2] Ogodo, A. C., Ugbogu, O. C., Ugbogu, A. E. and Ezeonu, C. S., 2015. Production of mixed fruit (pawpaw, banana and watermelon) wine using Saccharomyces cerevisiae isolated from palm wine. SpringerPlus, 4(1), pp. 1-11.
[3] Isitua, C. C. and Ibeh, I. N., 2010. Novel method of wine production from banana (Musa acuminata) and pineapple (Ananas comosus) wastes. African journal of Biotechnology, 9(44), pp. 7521-7524.
[4] Alemu, D. and Dagnew, A., 2008. Banana markets in Ethiopia. Ethiopian Institute of Agricultural Research.
[5] Tamrakar, K., Lama, A., Dhakal, B., Adhikari, L., Shrestha, M. and Amatya, J., 2020. Qualitative analysis of wine prepared from banana and orange. Int J Food Sci Nutr, 5(1), p 7 p. 60-63.
[6] Saranraj, P., Sivasakthivelan, P. and Naveen, M., 2017. Fermentation of fruit wine and its quality analysis: A review. Australian Journal of Science and Technology, 1(2), pp. 85- 97.
[7] Okiemute, E. and Edward, I., 2011. Studies of wine produced from banana (Musa sapientum). International Journal of Biotechnology and Molecular Biology Research, 2(12), pp. 209-214.
[8] Maheswari, T. U., Karuppaiya, M., Subhagar, S. and Rahul, R., 2020. Potent inhibitory action of Banana wine polyphenols on colon cancer cells (HCT-15). Research J. Pharm. and Tech, 13(11), pp. 5387-5390.
[9] Swami, S. B., Thakor, N. J. and Divate, A. D., 2014. Fruit wine production: a review. Journal of Food Research and Technology, 2(3), pp. 93-100.
[10] Martinho, V. J. P. D., 2019. Historical records of wine: Highlighting the old wine world.
[11] Byarugaba-Bazirake, G. W., 2008. The effect of enzymatic processing on banana juice and wine (Doctoral dissertation, Stellenbosch: Stellenbosch University).
[12] Shyamsunder mishra Srinivas Tadepalli., 2020. Production of Wine from banana. Waffen-Und Kostumkunde Journal, pp. 27-47.
[13] Mills, D. A., Phister, T., Neeley, E. and Johannsen, E., 2008. Wine fermentation. In Molecular techniques in the microbial ecology of fermented foods (pp. 162-192).
[14] J. H., Varela, C., Pretorius, I. S. and Agosin, E., 2007. Influence of wine fermentation temperature on the synthesis of yeast-derived volatile aroma compounds. Applied microbiology and biotechnology, 77(3), pp. 675-687.
[15] Zamora, F., 2009. Biochemistry of alcoholic fermentation. In Wine chemistry and biochemistry (pp. 3-26). Springer, New York, NY.
[16] Boulton, R. B., Singleton, V. L., Bisson, L. F. and Kunkee, R. E., 1999. Yeast and biochemistry of ethanol fermentation. In Principles and practices of winemaking (pp. 102-192). Springer, Boston, MA.
[17] Fleet, G. H., 2008. Wine yeasts for the future. FEMS yeast research, 8(7), pp. 979-995.
[18] Okafor, D. C., Ihediohanma, N. C., Abolude, D. S., Onuegbu, N. C., Osuji, C. M. and Ofoedu, C. E., 2014. Physico-chemical and Sensory Acceptability of Soursop (Annonamuricata) Wine. Int J Life Sci, 3, pp. 163-169.
[19] Sadler, G. D. and Murphy, P. A., 2010. pH and titratable acidity. In Food analysis (pp. 219-238). Springer, Boston, MA.
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    Boggale, W. R. (2025). Production and Comprehensive Characterization of Banana Fruit Wine Using Saccharomyces Cerevisiae. American Journal of Applied and Industrial Chemistry, 9(2), 74-79. https://doi.org/10.11648/j.ajaic.20250902.15

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    ACS Style

    Boggale, W. R. Production and Comprehensive Characterization of Banana Fruit Wine Using Saccharomyces Cerevisiae. Am. J. Appl. Ind. Chem. 2025, 9(2), 74-79. doi: 10.11648/j.ajaic.20250902.15

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    AMA Style

    Boggale WR. Production and Comprehensive Characterization of Banana Fruit Wine Using Saccharomyces Cerevisiae. Am J Appl Ind Chem. 2025;9(2):74-79. doi: 10.11648/j.ajaic.20250902.15

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  • @article{10.11648/j.ajaic.20250902.15,
      author = {Wabi Reggassa Boggale},
      title = {Production and Comprehensive Characterization of Banana Fruit Wine Using Saccharomyces Cerevisiae},
      journal = {American Journal of Applied and Industrial Chemistry},
      volume = {9},
      number = {2},
      pages = {74-79},
      doi = {10.11648/j.ajaic.20250902.15},
      url = {https://doi.org/10.11648/j.ajaic.20250902.15},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajaic.20250902.15},
      abstract = {Bananas (Musa spp.) are a globally cultivated fruit valued for their flavor and rich nutritional content (high in sugars, potassium and B-vitamins). Because ripe bananas are highly perishable, converting excess or overripe bananas into wine can reduce waste and add value. Fruit wines are produced by fermenting fruit juices with yeast, turning sugars into ethanol, carbon dioxide and minor by-products that give wine its aroma and preserve it. In particular, the high sugar/carbohydrate content of banana makes it an excellent substrate for fermentation. Thus banana wine production has emerged as an attractive alternative to grape wine in regions where grapes are scarce: it harnesses surplus fruit, yields a vitamin-rich beverage, and extends shelf life through fermentation. In this study, ripe banana pulp was cleaned, mashed and blended with water, sugar and lemon juice, then inoculated with baker’s yeast (Saccharomyces cerevisiae) and fermented anaerobically for about six days. Fermentation progress was monitored by measuring pH, titratable acidity, specific gravity, total dissolved solids (TDS) and ethanol content at regular intervals. These analytical assays are standard for fruit wine characterization. The yeast consumes banana sugars to produce ethanol and CO2; as fermentation proceeded we observed vigorous bubbling (CO2 release) and viscosity reduction, as expected for Saccharomyces fermentation. The fermenting banana must showed the typical trends of wine fermentation. The pH fell markedly (from about 5.0 initially to ~2.0 by the end), reflecting increased organic acid production, while titratable acidity rose to ~1.6 g/100 mL (as lactic/acetic acid equivalents). Specific gravity declined (from ~0.983 to ~0.982), indicating sugar depletion and ethanol formation. Correspondingly, ethanol content increased steadily and reached about 14% v/v by day six. Observed ethanol rising to ~15% as pH dropped into the low-3 range during fruit fermentation. A clear, amber banana wine was obtained after racking; it exhibited the characteristic aroma and flavor of banana and acceptable clarity.},
     year = {2025}
    }
    

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  • TY  - JOUR
    T1  - Production and Comprehensive Characterization of Banana Fruit Wine Using Saccharomyces Cerevisiae
    AU  - Wabi Reggassa Boggale
    Y1  - 2025/12/20
    PY  - 2025
    N1  - https://doi.org/10.11648/j.ajaic.20250902.15
    DO  - 10.11648/j.ajaic.20250902.15
    T2  - American Journal of Applied and Industrial Chemistry
    JF  - American Journal of Applied and Industrial Chemistry
    JO  - American Journal of Applied and Industrial Chemistry
    SP  - 74
    EP  - 79
    PB  - Science Publishing Group
    SN  - 2994-7294
    UR  - https://doi.org/10.11648/j.ajaic.20250902.15
    AB  - Bananas (Musa spp.) are a globally cultivated fruit valued for their flavor and rich nutritional content (high in sugars, potassium and B-vitamins). Because ripe bananas are highly perishable, converting excess or overripe bananas into wine can reduce waste and add value. Fruit wines are produced by fermenting fruit juices with yeast, turning sugars into ethanol, carbon dioxide and minor by-products that give wine its aroma and preserve it. In particular, the high sugar/carbohydrate content of banana makes it an excellent substrate for fermentation. Thus banana wine production has emerged as an attractive alternative to grape wine in regions where grapes are scarce: it harnesses surplus fruit, yields a vitamin-rich beverage, and extends shelf life through fermentation. In this study, ripe banana pulp was cleaned, mashed and blended with water, sugar and lemon juice, then inoculated with baker’s yeast (Saccharomyces cerevisiae) and fermented anaerobically for about six days. Fermentation progress was monitored by measuring pH, titratable acidity, specific gravity, total dissolved solids (TDS) and ethanol content at regular intervals. These analytical assays are standard for fruit wine characterization. The yeast consumes banana sugars to produce ethanol and CO2; as fermentation proceeded we observed vigorous bubbling (CO2 release) and viscosity reduction, as expected for Saccharomyces fermentation. The fermenting banana must showed the typical trends of wine fermentation. The pH fell markedly (from about 5.0 initially to ~2.0 by the end), reflecting increased organic acid production, while titratable acidity rose to ~1.6 g/100 mL (as lactic/acetic acid equivalents). Specific gravity declined (from ~0.983 to ~0.982), indicating sugar depletion and ethanol formation. Correspondingly, ethanol content increased steadily and reached about 14% v/v by day six. Observed ethanol rising to ~15% as pH dropped into the low-3 range during fruit fermentation. A clear, amber banana wine was obtained after racking; it exhibited the characteristic aroma and flavor of banana and acceptable clarity.
    VL  - 9
    IS  - 2
    ER  - 

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