The most basic application of protein hydrolysis is to provide nitrogen for bacterial culture media. Given that approximately 200 million doses of Clostridium bacterium vaccines are consumed annually in Iran, and 16 tons of hydrolyzed protein [peptone] are required to produce these doses of vaccines. Currently, this need is met through imports, so it is essential to obtain the technical knowledge to produce peptone from various inexpensive sources in the country. Considering the development of vermicompost worm breeding farms, their cheapness, availability, and high protein content, protein hydrolysis of this animal was performed. Worm samples were purchased from farms around Mashhad. To empty the digestive system of the worms, they were placed in a container with clean water and sand for 20 hours. Then, homogenizing them in water for 30 minutes provided a homogeneous and uniform suspension. Following this, fat was removed from the protein tissue with chloroform for two 30-minute periods at 25°C and 60°C. Protein hydrolysis was performed using the vapor phase of six-normal hydrochloric acid at 110°C for 20 hours. Complete drying of the sample was performed for 3 hours under vacuum conditions with a single pressure at 70°C. In this study, three grams of peptone were produced from an initial amount of 10 grams of vermicompost worms. This amount of hydrolyzed protein produced was equal to 30% of the initial weight of the worms.
| Published in | American Journal of Biomedical and Life Sciences (Volume 13, Issue 6) |
| DOI | 10.11648/j.ajbls.20251306.14 |
| Page(s) | 142-147 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2025. Published by Science Publishing Group |
Peptone Production, Amino Acids, Protein Sources, Acid Hydrolysis, Worm Protein
| [1] | Medina A, Cova J, Vielma R, Pujic P, Carlos M, Torres J. Immunological and chemical analysis of proteins from Eisenia fetida earthworm. Food and Agricultural Immunology. 2003; 15(3-4): 255-63. |
| [2] | Pasupuleti VK, Braun S. State of the art manufacturing of protein hydrolysates. Protein hydrolysates in biotechnology. 2010: 11-32. |
| [3] | Noruzy Mogadam H, Banaei A. Production of hydrolyzed protein from vermicompost worms Eisenia foetida for use in vaccine production process. Veterinary Research & Biological Products. 2022; 35(2): 88-94. |
| [4] | Mustățea G, Ungureanu EL, Iorga E. Protein acidic hydrolysis for amino acids analysis in food-progress over time: a short review. |
| [5] | Hou Y, Wu Z, Dai Z, Wang G, Wu G. Protein hydrolysates in animal nutrition: Industrial production, bioactive peptides, and functional significance. Bioactive Peptides from Food. 2022: 209-32. |
| [6] | Cock LS, Guerrero CAR, Restrepo MAR. The use of earthworm flour for lactic acid biomass production. African Journal of Biotechnology. 2013; 12(40). |
| [7] | Wisuthiphaet N, Kongruang S, Chamcheun C. Production of fish protein hydrolysates by acid and enzymatic hydrolysis. Journal of Medical and Bioengineering. 2015; 4(6). |
| [8] | Mongkonkamthorn N, Malila Y, Yarnpakdee S, Makkhun S, Regenstein JM, Wangtueai S. Production of protein hydrolysate containing antioxidant and angiotensin-I-converting enzyme [ACE] inhibitory activities from tuna (Katsuwonus pelamis) blood. Processes. 2020; 8(11): 1518. |
| [9] | Saputra D, Nurhayati T. Production of fish hydrolysates protein from waste of fish carp [Cyprinus carpio] by enzymatic hydrolysis. ComTech: Computer, Mathematics and Engineering Applications. 2016; 7(1): 11-8. |
| [10] | Bernardi DM, PARIS LDd, Dieterich F, Boscolo WR, Sary C, Signor A, et al. Production of hydrolysate from processed Nile tilapia [Oreochromis niloticus] residues and assessment of its antioxidant activity. Food Science and Technology. 2016; 36: 709-16. |
| [11] | Taheri A, Anvar S, Ahari H, Fogliano V. Comparison the functional properties of protein Hydrolysates from poultry byproducts and rainbow trout. Iranian journal of fisheries sciences. 2013; 12(1): 154-69. |
| [12] | Gahadkchee H. Preparation of pepton soya protein, using in vaccine media. Final Report Res Plan. 1997.[In persion] |
| [13] | Clark J Hydrolysis Hydrolysis of Proteins. |
| [14] | Moreno-Hernandez JM, Benítez-García I, Mazorra-Manzano MA, Ramírez-Suarez JC, Sanchez E. Strategies for production, characterization and application of protein-based biostimulants in agriculture: A review. Chilean journal of agricultural research. 2020; 80(2): 274-89. |
| [15] | Hydrolysis to Hydrolysate. Becton, Dickinson and Company (2009). Available from: |
| [16] | McCarthy AL, O’Callaghan YC, O’Brien NM. Protein hydrolysates from agricultural crops—bioactivity and potential for functional food development. Agriculture. 2013; 3(1): 112-30. |
| [17] |
Al-bahri MB, AL-Naimi SA, Ahammed SH. The optimum conditions for production of soya peptone by acidic hydrolysis of soya proteins. Al-Khwarizmi Engineering Journal. 2009; 5(1): 1-19.
https://alkej.uobaghdad.edu.iq/index.php/alkej/article/view/516 |
| [18] | Grieshop CM, Kadzere CT, Clapper GM, Flickinger EA, Bauer LL, Frazier RL, et al. Chemical and nutritional characteristics of United States soybeans and soybean meals. Journal of Agricultural and Food Chemistry. 2003; 51(26): 7684-91. |
APA Style
Moghadam, H. N. (2025). Laboratory Preparation of Eisenia Fetida-Based Peptone as a Nutrient Source for Vaccine Manufacture. American Journal of Biomedical and Life Sciences, 13(6), 142-147. https://doi.org/10.11648/j.ajbls.20251306.14
ACS Style
Moghadam, H. N. Laboratory Preparation of Eisenia Fetida-Based Peptone as a Nutrient Source for Vaccine Manufacture. Am. J. Biomed. Life Sci. 2025, 13(6), 142-147. doi: 10.11648/j.ajbls.20251306.14
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Download@article{10.11648/j.ajbls.20251306.14,
author = {Hossein Noruzy Moghadam},
title = {Laboratory Preparation of Eisenia Fetida-Based Peptone as a Nutrient Source for Vaccine Manufacture},
journal = {American Journal of Biomedical and Life Sciences},
volume = {13},
number = {6},
pages = {142-147},
doi = {10.11648/j.ajbls.20251306.14},
url = {https://doi.org/10.11648/j.ajbls.20251306.14},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbls.20251306.14},
abstract = {The most basic application of protein hydrolysis is to provide nitrogen for bacterial culture media. Given that approximately 200 million doses of Clostridium bacterium vaccines are consumed annually in Iran, and 16 tons of hydrolyzed protein [peptone] are required to produce these doses of vaccines. Currently, this need is met through imports, so it is essential to obtain the technical knowledge to produce peptone from various inexpensive sources in the country. Considering the development of vermicompost worm breeding farms, their cheapness, availability, and high protein content, protein hydrolysis of this animal was performed. Worm samples were purchased from farms around Mashhad. To empty the digestive system of the worms, they were placed in a container with clean water and sand for 20 hours. Then, homogenizing them in water for 30 minutes provided a homogeneous and uniform suspension. Following this, fat was removed from the protein tissue with chloroform for two 30-minute periods at 25°C and 60°C. Protein hydrolysis was performed using the vapor phase of six-normal hydrochloric acid at 110°C for 20 hours. Complete drying of the sample was performed for 3 hours under vacuum conditions with a single pressure at 70°C. In this study, three grams of peptone were produced from an initial amount of 10 grams of vermicompost worms. This amount of hydrolyzed protein produced was equal to 30% of the initial weight of the worms.},
year = {2025}
}
TY - JOUR T1 - Laboratory Preparation of Eisenia Fetida-Based Peptone as a Nutrient Source for Vaccine Manufacture AU - Hossein Noruzy Moghadam Y1 - 2025/12/19 PY - 2025 N1 - https://doi.org/10.11648/j.ajbls.20251306.14 DO - 10.11648/j.ajbls.20251306.14 T2 - American Journal of Biomedical and Life Sciences JF - American Journal of Biomedical and Life Sciences JO - American Journal of Biomedical and Life Sciences SP - 142 EP - 147 PB - Science Publishing Group SN - 2330-880X UR - https://doi.org/10.11648/j.ajbls.20251306.14 AB - The most basic application of protein hydrolysis is to provide nitrogen for bacterial culture media. Given that approximately 200 million doses of Clostridium bacterium vaccines are consumed annually in Iran, and 16 tons of hydrolyzed protein [peptone] are required to produce these doses of vaccines. Currently, this need is met through imports, so it is essential to obtain the technical knowledge to produce peptone from various inexpensive sources in the country. Considering the development of vermicompost worm breeding farms, their cheapness, availability, and high protein content, protein hydrolysis of this animal was performed. Worm samples were purchased from farms around Mashhad. To empty the digestive system of the worms, they were placed in a container with clean water and sand for 20 hours. Then, homogenizing them in water for 30 minutes provided a homogeneous and uniform suspension. Following this, fat was removed from the protein tissue with chloroform for two 30-minute periods at 25°C and 60°C. Protein hydrolysis was performed using the vapor phase of six-normal hydrochloric acid at 110°C for 20 hours. Complete drying of the sample was performed for 3 hours under vacuum conditions with a single pressure at 70°C. In this study, three grams of peptone were produced from an initial amount of 10 grams of vermicompost worms. This amount of hydrolyzed protein produced was equal to 30% of the initial weight of the worms. VL - 13 IS - 6 ER -
Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Mashhad, Iran
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