Association of NLRP3 rs4612666 Gene Polymorphism with Helicobacter Pylori Infection Susceptibility a Case-Control Study Using Molecular and Statistical Analysis

Liyan Liu; Chaoling Shi; Yixuan Lang; Bo Zhang; Zhonghuang Cong; Li Yang; Chunlei Dai; Guotao Qin; Zhonghui Wang; Tieliang Sun; Jie Lei

doi:doi:10.11648/j.ajhr.20251306.16

Research Article |

| Peer-Reviewed

Association of NLRP3 rs4612666 Gene Polymorphism with Helicobacter Pylori Infection Susceptibility a Case-Control Study Using Molecular and Statistical Analysis

Liyan Liu

, Chaoling Shi

, Yixuan Lang

, Bo Zhang

, Zhonghuang Cong

, Li Yang, Chunlei Dai, Guotao Qin, Zhonghui Wang, Tieliang Sun, Jie Lei^*

Published in American Journal of Health Research (Volume 13, Issue 6)

Received: 27 October 2025 Accepted: 19 November 2025 Published: 11 December 2025

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Abstract

Helicobacter pylori infection is a major global health concern, associated with various gastrointestinal disorders such as peptic ulcers and gastric cancer, which result in significant morbidity and economic burden on healthcare systems. This study investigates the relationship between the NLRP3 rs4612666 gene polymorphism and susceptibility to H. pylori infection using a case-control design that involved 200 people. We employed molecular techniques and statistical analyses to evaluate the distributions of genotypes and alleles. Our findings indicated no significant difference in the distribution of NLRP3 rs4612666 genotypes between the infected group (C/C: 35.0%, C/T: 51.0%, T/T: 14.0%) and the non-infected group (C/C: 24.0%, C/T: 50.0%, T/T: 26.0%) with a p-value of 0.059. However, we did observe a statistically significant difference in allele frequencies, with allele C found in 60.5% of the infected group compared to 49.0% in the non-infected group (p=0.021), suggesting that allele C may be linked to a higher susceptibility to H. pylori infection. Despite these insights, the study has limitations, including a small sample size and a lack of clinical validation, highlighting the necessity for further research with larger cohorts to verify these associations. This research enhances our understanding of the genetic factors contributing to H. pylori infection, which could inform future diagnostic and therapeutic approaches, and emphasizes the need to explore additional genetic markers to deepen our understanding of the disease's pathogenesis.

Published in	American Journal of Health Research (Volume 13, Issue 6)
DOI	10.11648/j.ajhr.20251306.16
Page(s)	338-342
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2025. Published by Science Publishing Group

Keywords

Association, NLRP3 rs4612666, Gene Polymorphism, Helicobacter pylori Infection

References

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[2]	Fischbach W, Malfertheiner P. Helicobacter Pylori Infection. Dtsch Arztebl Int. 2018; 115(25): 429-436. https://doi.org/10.3238/arztebl.2018.0429
[3]	Liou J-M, Chen C-C, Chang C-Y, et al. Comparison of the accuracy of non-invasive tests for active Helicobacter pylori infection: a large-scale, multicenter study. Lancet Gastroenterol Hepatol. 2023; 8(6):524-535. https://doi.org/10.1016/S2468-1253(23)00050-7
[4]	Cho J, Prashar A, Jones NL, Moss SF. Helicobacter pylori Infection. Gastroenterol Clin North Am. 2021; 50(2): 261-282. https://doi.org/10.1016/j.gtc.2021.02.001
[5]	González Peña T, Huang M. Genetic Predisposition for Gynecologic Cancers. Clin Obstet Gynecol. 2024; 67(4): 660-665. https://doi.org/10.1097/GRF.0000000000000894
[6]	Malfertheiner P, Camargo MC, El-Omar E, et al. Helicobacter pylori infection. Nat Rev Dis Primers. 2023; 9(1): 19. Published 2023 Apr 20. https://doi.org/10.1038/s41572-023-00431-8
[7]	Lu B, Shi J, Cheng T, et al. Chemokine ligand 14 correlates with immune cell infiltration in the gastric cancer microenvironment in predicting unfavorable prognosis. Front Pharmacol. 15: 1397656. Published 2024 None. https://doi.org/10.3389/fphar.2024.1397656
[8]	Chen W, Wu CS, Liu CC, et al. In-Hospital Use of Long-Acting Injectable Antipsychotics and Readmission Risk in Patients With First-Admission Schizophrenia in Taiwan. JAMA Netw Open. 2024; 7(6): e2417006. Published 2024 Jun 3. https://doi.org/10.1001/jamanetworkopen.2024.17006
[9]	Ashkar Daw M, Azrad M, Peretz A. Associations between biofilm formation and virulence factors among clinical Helicobacter pylori isolates. Microb Pathog. 196:106977. https://doi.org/10.1016/j.micpath.2024.106977
[10]	Graham DY. Helicobacter pylori. Curr Top Microbiol Immunol. 445: 127-154. https://doi.org/10.1007/82_2021_235
[11]	Tshibangu-Kabamba E, and Yamaoka Y. Helicobacter pylori infection and antibiotic resistance from a human perspective. Nat Rev Gastroenterol Hepatol. 2024; 21(3): 184-200. https://doi.org/10.1038/s41575-023-00883-z
[12]	Gantuya B, and Yamaoka Y. The interplay between host genetic factors, Helicobacter pylori virulence, and environmental influences in determining clinical outcomes: an updated review. Nat Rev Gastroenterol Hepatol. 2024; 22(1): 45-62. https://doi.org/10.1038/s41575-023-00882-5
[13]	Sjomina O, et al. Global prevalence of Helicobacter pylori infection and its association with dietary patterns and other modifiable risk factors: a systematic analysis. Lancet Gastroenterol Hepatol.2023;8(11):1011-1025. https://doi.org/10.1016/S2468-1253(23)00234-8
[14]	Malfertheiner P, et al. Management of Helicobacter pylori infection: the Maastricht VI/Florence consensus report. Gut. 2022; 71(9): 1724-1762. https://doi.org/10.1136/gutjnl-2022-327745
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APA Style

Liu, L., Shi, C., Lang, Y., Zhang, B., Cong, Z., et al. (2025). Association of NLRP3 rs4612666 Gene Polymorphism with Helicobacter Pylori Infection Susceptibility a Case-Control Study Using Molecular and Statistical Analysis. American Journal of Health Research, 13(6), 338-342. https://doi.org/10.11648/j.ajhr.20251306.16

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ACS Style

Liu, L.; Shi, C.; Lang, Y.; Zhang, B.; Cong, Z., et al. Association of NLRP3 rs4612666 Gene Polymorphism with Helicobacter Pylori Infection Susceptibility a Case-Control Study Using Molecular and Statistical Analysis. Am. J. Health Res. 2025, 13(6), 338-342. doi: 10.11648/j.ajhr.20251306.16

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AMA Style

Liu L, Shi C, Lang Y, Zhang B, Cong Z, et al. Association of NLRP3 rs4612666 Gene Polymorphism with Helicobacter Pylori Infection Susceptibility a Case-Control Study Using Molecular and Statistical Analysis. Am J Health Res. 2025;13(6):338-342. doi: 10.11648/j.ajhr.20251306.16

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@article{10.11648/j.ajhr.20251306.16,
  author = {Liyan Liu and Chaoling Shi and Yixuan Lang and Bo Zhang and Zhonghuang Cong and Li Yang and Chunlei Dai and Guotao Qin and Zhonghui Wang and Tieliang Sun and Jie Lei},
  title = {Association of NLRP3 rs4612666 Gene Polymorphism with Helicobacter Pylori Infection Susceptibility a Case-Control Study Using Molecular and Statistical Analysis},
  journal = {American Journal of Health Research},
  volume = {13},
  number = {6},
  pages = {338-342},
  doi = {10.11648/j.ajhr.20251306.16},
  url = {https://doi.org/10.11648/j.ajhr.20251306.16},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajhr.20251306.16},
  abstract = {Helicobacter pylori infection is a major global health concern, associated with various gastrointestinal disorders such as peptic ulcers and gastric cancer, which result in significant morbidity and economic burden on healthcare systems. This study investigates the relationship between the NLRP3 rs4612666 gene polymorphism and susceptibility to H. pylori infection using a case-control design that involved 200 people. We employed molecular techniques and statistical analyses to evaluate the distributions of genotypes and alleles. Our findings indicated no significant difference in the distribution of NLRP3 rs4612666 genotypes between the infected group (C/C: 35.0%, C/T: 51.0%, T/T: 14.0%) and the non-infected group (C/C: 24.0%, C/T: 50.0%, T/T: 26.0%) with a p-value of 0.059. However, we did observe a statistically significant difference in allele frequencies, with allele C found in 60.5% of the infected group compared to 49.0% in the non-infected group (p=0.021), suggesting that allele C may be linked to a higher susceptibility to H. pylori infection. Despite these insights, the study has limitations, including a small sample size and a lack of clinical validation, highlighting the necessity for further research with larger cohorts to verify these associations. This research enhances our understanding of the genetic factors contributing to H. pylori infection, which could inform future diagnostic and therapeutic approaches, and emphasizes the need to explore additional genetic markers to deepen our understanding of the disease's pathogenesis.},
 year = {2025}
}

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TY  - JOUR
T1  - Association of NLRP3 rs4612666 Gene Polymorphism with Helicobacter Pylori Infection Susceptibility a Case-Control Study Using Molecular and Statistical Analysis
AU  - Liyan Liu
AU  - Chaoling Shi
AU  - Yixuan Lang
AU  - Bo Zhang
AU  - Zhonghuang Cong
AU  - Li Yang
AU  - Chunlei Dai
AU  - Guotao Qin
AU  - Zhonghui Wang
AU  - Tieliang Sun
AU  - Jie Lei
Y1  - 2025/12/11
PY  - 2025
N1  - https://doi.org/10.11648/j.ajhr.20251306.16
DO  - 10.11648/j.ajhr.20251306.16
T2  - American Journal of Health Research
JF  - American Journal of Health Research
JO  - American Journal of Health Research
SP  - 338
EP  - 342
PB  - Science Publishing Group
SN  - 2330-8796
UR  - https://doi.org/10.11648/j.ajhr.20251306.16
AB  - Helicobacter pylori infection is a major global health concern, associated with various gastrointestinal disorders such as peptic ulcers and gastric cancer, which result in significant morbidity and economic burden on healthcare systems. This study investigates the relationship between the NLRP3 rs4612666 gene polymorphism and susceptibility to H. pylori infection using a case-control design that involved 200 people. We employed molecular techniques and statistical analyses to evaluate the distributions of genotypes and alleles. Our findings indicated no significant difference in the distribution of NLRP3 rs4612666 genotypes between the infected group (C/C: 35.0%, C/T: 51.0%, T/T: 14.0%) and the non-infected group (C/C: 24.0%, C/T: 50.0%, T/T: 26.0%) with a p-value of 0.059. However, we did observe a statistically significant difference in allele frequencies, with allele C found in 60.5% of the infected group compared to 49.0% in the non-infected group (p=0.021), suggesting that allele C may be linked to a higher susceptibility to H. pylori infection. Despite these insights, the study has limitations, including a small sample size and a lack of clinical validation, highlighting the necessity for further research with larger cohorts to verify these associations. This research enhances our understanding of the genetic factors contributing to H. pylori infection, which could inform future diagnostic and therapeutic approaches, and emphasizes the need to explore additional genetic markers to deepen our understanding of the disease's pathogenesis.
VL  - 13
IS  - 6
ER  -

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Author Information

Liyan Liu

Jilin Province FAW General Hospital, Changchun, China

http://orcid.org/0009-0003-3690-2882
Chaoling Shi

Jilin Province FAW General Hospital, Changchun, China

http://orcid.org/0009-0004-7495-6811
Yixuan Lang

Jilin Province FAW General Hospital, Changchun, China

http://orcid.org/0009-0004-1107-5005
Bo Zhang

Jilin Province FAW General Hospital, Changchun, China

http://orcid.org/0009-0000-4511-5029
Zhonghuang Cong

Jilin Province FAW General Hospital, Changchun, China

http://orcid.org/0009-0003-4056-4628
Li Yang

Jilin Province FAW General Hospital, Changchun, China
Chunlei Dai

Jilin Province FAW General Hospital, Changchun, China
Guotao Qin

Jilin Province FAW General Hospital, Changchun, China
Zhonghui Wang

Jilin Province FAW General Hospital, Changchun, China
Tieliang Sun

Jilin Province FAW General Hospital, Changchun, China
Jie Lei

Jilin Province FAW General Hospital, Changchun, China

Contact Email

http://orcid.org/0009-0008-1786-6990

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Plain Text BibTeX RIS

APA Style

Liu, L., Shi, C., Lang, Y., Zhang, B., Cong, Z., et al. (2025). Association of NLRP3 rs4612666 Gene Polymorphism with Helicobacter Pylori Infection Susceptibility a Case-Control Study Using Molecular and Statistical Analysis. American Journal of Health Research, 13(6), 338-342. https://doi.org/10.11648/j.ajhr.20251306.16

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ACS Style

Liu, L.; Shi, C.; Lang, Y.; Zhang, B.; Cong, Z., et al. Association of NLRP3 rs4612666 Gene Polymorphism with Helicobacter Pylori Infection Susceptibility a Case-Control Study Using Molecular and Statistical Analysis. Am. J. Health Res. 2025, 13(6), 338-342. doi: 10.11648/j.ajhr.20251306.16

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AMA Style

Liu L, Shi C, Lang Y, Zhang B, Cong Z, et al. Association of NLRP3 rs4612666 Gene Polymorphism with Helicobacter Pylori Infection Susceptibility a Case-Control Study Using Molecular and Statistical Analysis. Am J Health Res. 2025;13(6):338-342. doi: 10.11648/j.ajhr.20251306.16

Copy | Download

@article{10.11648/j.ajhr.20251306.16,
  author = {Liyan Liu and Chaoling Shi and Yixuan Lang and Bo Zhang and Zhonghuang Cong and Li Yang and Chunlei Dai and Guotao Qin and Zhonghui Wang and Tieliang Sun and Jie Lei},
  title = {Association of NLRP3 rs4612666 Gene Polymorphism with Helicobacter Pylori Infection Susceptibility a Case-Control Study Using Molecular and Statistical Analysis},
  journal = {American Journal of Health Research},
  volume = {13},
  number = {6},
  pages = {338-342},
  doi = {10.11648/j.ajhr.20251306.16},
  url = {https://doi.org/10.11648/j.ajhr.20251306.16},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajhr.20251306.16},
  abstract = {Helicobacter pylori infection is a major global health concern, associated with various gastrointestinal disorders such as peptic ulcers and gastric cancer, which result in significant morbidity and economic burden on healthcare systems. This study investigates the relationship between the NLRP3 rs4612666 gene polymorphism and susceptibility to H. pylori infection using a case-control design that involved 200 people. We employed molecular techniques and statistical analyses to evaluate the distributions of genotypes and alleles. Our findings indicated no significant difference in the distribution of NLRP3 rs4612666 genotypes between the infected group (C/C: 35.0%, C/T: 51.0%, T/T: 14.0%) and the non-infected group (C/C: 24.0%, C/T: 50.0%, T/T: 26.0%) with a p-value of 0.059. However, we did observe a statistically significant difference in allele frequencies, with allele C found in 60.5% of the infected group compared to 49.0% in the non-infected group (p=0.021), suggesting that allele C may be linked to a higher susceptibility to H. pylori infection. Despite these insights, the study has limitations, including a small sample size and a lack of clinical validation, highlighting the necessity for further research with larger cohorts to verify these associations. This research enhances our understanding of the genetic factors contributing to H. pylori infection, which could inform future diagnostic and therapeutic approaches, and emphasizes the need to explore additional genetic markers to deepen our understanding of the disease's pathogenesis.},
 year = {2025}
}

Copy | Download

TY  - JOUR
T1  - Association of NLRP3 rs4612666 Gene Polymorphism with Helicobacter Pylori Infection Susceptibility a Case-Control Study Using Molecular and Statistical Analysis
AU  - Liyan Liu
AU  - Chaoling Shi
AU  - Yixuan Lang
AU  - Bo Zhang
AU  - Zhonghuang Cong
AU  - Li Yang
AU  - Chunlei Dai
AU  - Guotao Qin
AU  - Zhonghui Wang
AU  - Tieliang Sun
AU  - Jie Lei
Y1  - 2025/12/11
PY  - 2025
N1  - https://doi.org/10.11648/j.ajhr.20251306.16
DO  - 10.11648/j.ajhr.20251306.16
T2  - American Journal of Health Research
JF  - American Journal of Health Research
JO  - American Journal of Health Research
SP  - 338
EP  - 342
PB  - Science Publishing Group
SN  - 2330-8796
UR  - https://doi.org/10.11648/j.ajhr.20251306.16
AB  - Helicobacter pylori infection is a major global health concern, associated with various gastrointestinal disorders such as peptic ulcers and gastric cancer, which result in significant morbidity and economic burden on healthcare systems. This study investigates the relationship between the NLRP3 rs4612666 gene polymorphism and susceptibility to H. pylori infection using a case-control design that involved 200 people. We employed molecular techniques and statistical analyses to evaluate the distributions of genotypes and alleles. Our findings indicated no significant difference in the distribution of NLRP3 rs4612666 genotypes between the infected group (C/C: 35.0%, C/T: 51.0%, T/T: 14.0%) and the non-infected group (C/C: 24.0%, C/T: 50.0%, T/T: 26.0%) with a p-value of 0.059. However, we did observe a statistically significant difference in allele frequencies, with allele C found in 60.5% of the infected group compared to 49.0% in the non-infected group (p=0.021), suggesting that allele C may be linked to a higher susceptibility to H. pylori infection. Despite these insights, the study has limitations, including a small sample size and a lack of clinical validation, highlighting the necessity for further research with larger cohorts to verify these associations. This research enhances our understanding of the genetic factors contributing to H. pylori infection, which could inform future diagnostic and therapeutic approaches, and emphasizes the need to explore additional genetic markers to deepen our understanding of the disease's pathogenesis.
VL  - 13
IS  - 6
ER  -

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