Hydroxyapatite and Demineralized Bone Matrix from Marine Food Waste – A Possible Bone Implant

Rethinam Senthil; Sathyaraj Weslen Vedakumari; Thotapalli Parvathaleswara Sastry

doi:doi:10.11648/j.ajmsp.20180301.11

| Peer-Reviewed

Hydroxyapatite and Demineralized Bone Matrix from Marine Food Waste – A Possible Bone Implant

Rethinam Senthil, Sathyaraj Weslen Vedakumari, Thotapalli Parvathaleswara Sastry

Published in American Journal of Materials Synthesis and Processing (Volume 3, Issue 1)

Received: 28 September 2017 Accepted: 18 October 2017 Published: 18 January 2018

Views: Downloads:

Download PDF

Share This Article

Twitter
Linked In
Facebook

Abstract

In the present study, a novel bone implant (BI) was prepared using demineralized bone matrix (DBM) and hydroxyapatite (HA) isolated from Bluefin trevally (BT) bones, which was considered to be a marine industry food waste. Gelatin (GA) was used as a binder. Physico-chemical characterization and in vitro studies were carried out using this implant. Fourier transform infrared spectrum of BI exhibited the characteristic bands of all the three components viz., DBM, HA and GA, while scanning electron microscopic studies revealed the irregular shape of the particles. The mechanical properties of BI were also appreciable. In vitro studies were carried out using Human keratinocyte cell line (HaCaT), wherein MTT (3-(4,5-dimethylazol-2-yl)-2,5-diphenyl-tetrazolium bromide) assay proved the biocompatibility of BI. From the results obtained it could be stated that BI prepared from waste marine bones could serve as a promising biomaterial for bone tissue engineering applications.

Published in	American Journal of Materials Synthesis and Processing (Volume 3, Issue 1)
DOI	10.11648/j.ajmsp.20180301.11
Page(s)	1-6
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2018. Published by Science Publishing Group

Keywords

Fish Waste, Recycling, Bone Implant, Biomaterial

References

[1]	Senthil R, Vedakumari SW, Hemalatha T, Sumathi V, Gobi N, Sastry TP, New Approaches for the Effective Utilization of Fish Skin Wastes of Aluterus monoceros, J Earth Environ Health Sci, 2: 50-5, 2016.
[2]	Jayathilakan K, Sultana K, Radhakrishna K, Bawa AS, Utilization of byproducts and waste materials from meat, poultry and fish processing industries: a review, J Food Sci Technol. 49: 278-293, 2012.
[3]	Sankar S, Sekar S, Mohan R, Sunita R, Sundaraseelan J, Sastry TP, Preparation and partial characterization of collagen sheet from fish (Lates calcarifer) scales, Int J Biol Macromol, 42: 6–9, 2008.
[4]	Arvanitoyannis I, Kassaveti A, Fish industry waste: Treatments, environmental impacts, current and potential uses, Int J Food Sci Technol, 43: 726-45, 2007.
[5]	Noorjahan SE, Sastry TP, Physiologically Clotted Fibrin-Calcined Bone Composite—A Possible Bone Graft Substitute, J Biomed Mater Res B, 75B: 343-350, 2005.
[6]	Sundaraseelan J, Sastry TP, Fabrication of a Biomimetic Compound Containing Nano Hydroxyapatite—Demineralised Bone Matrix, J Biomed Nanotechnol, 3: 1–5, 2007.
[7]	Manjubala I, Sivakumar M, Sureshkumar RV, Sastry TP, Bioactivity and osseointegration study of calcium phosphate ceramic of different chemical composition, J Biomed Mater Res, 63: 200-208, 2002.
[8]	Saffar JF, Colombier ML, Datenville R, Bone formation in tricalcium phosphate filled periodontal infrabony lesions. Histological observations in humans, J Periodontol, 61: 209–216, 1990.
[9]	Amit Kumar N, Hydroxyapatite Synthesis Methodologies: An Overview, Int J Chem Tech Res, 2: 903-907, 2010.
[10]	Guizzardi S, Montanari C, Migliaccio S, Strocchi R, Solmi R, Martin D, Ruggeri A, Qualitative assessment of natural apatite in vitro and in vivo, J Biomed Mater Res A, 53: 227-234, 2000.
[11]	Kang YH, Kim HM, Byun JH, KimUK, Sung IY, Cho YC, Park BW, Stability of simultaneously placed dental implants with autologous bone grafts harvested from the iliac crest or intraoral jaw bone, BMC Oral Health, 15: 3-11, 2015.
[12]	Gruskin E, Doll BA, William Futrell F, Schmitz JP, Hollinger JO, Demineralized bone matrix in bone repair: History and use, Adv Drug Deliv Rev, 64: 1063–1077, 2012.
[13]	Saraswathy G, Pal S, Rose C, Sastry TP, A novel bio-inorganic bone implant containing deglued bone, chitosan and gelatin, Bull Mater Sci, 24: 415–420, 2001.
[14]	Krithiga G, Antaryami J, Selvamani P, Sastry TP, In vitro study on biomineralization of biphasic calcium phosphate biocomposite crosslinked with hydrolysable tannins of Terminalia chebula, Bull Mater Sci, 34: 589-594, 2011.
[15]	Krithiga G, Kumar BS, Divya SM, Sastry TP, Influence of mineral phase in mineralization of a biocomposite containing chitosan, demineralized bone matrix and bone ash—in vitro study, Bull Mater Sci, 37: 729–733, 2014.
[16]	Zhang R, Ma PX, Poly(α-hydroxyl acids)/hydroxyapatite porous composites for bone-tissue engineering. I. Preparation and morphology, J Biomed Mater Res, 44: 446-455, 1999.
[17]	Jitendra P, Rajam AM, Kalaivani T, Mandal AB, Rose C, Collagen based silver nanoparticles for biological applications, Appl Mater Interfaces, 5: 7291-7298, 2013.
[18]	Jianguo L, Haihong L, Malena S, Characterization of calcium phosphates precipitated from simulated body fluid of different buffering capacities, Biomaterials, 18: 743-747, 1997.
[19]	Arockianathan PM, Sekar S, Kumaran B, Sastry TP, Preparation, characterization and evaluation of biocomposite films containing chitosan and sago starch impregnated with silver nanoparticles, Int J Biol Macromol, 50: 939-946, 2012.
[20]	Ucker BET, Cottel CM, Auyering RCV, Spector M, Nicollas GH, Pre-conditioning and dual constant composition dissolution kinetics of pulsed laser deposited hydroxyapatite thin films on silicon substrates, Biomaterials, 17: 631-637, 1996.
[21]	Plepis AMG, Goissis G, Das-Gupta DK, Dielectric and pyroelectric characterization of anionic and native collagen, Polym Eng Sci, 36: 2932-2938, 1996.
[22]	Das D, Zhang Z, Winkler T, Mour M, Gunter GI, Morlock MM, Machens HG, Schilling AF, Bioresorption and Degradation of Biomaterials, Adv Biochem Engin Biotech, 126: 317-333, 2011.
[23]	Dohan Ehrenfest DM, Coelho PG, Kang BS, Sul YT, Albrektsson T, Classification of osseointegrated implant surfaces: materials, chemistry and topography, Trend Biotech, 28: 198-206, 2010.
[24]	Sopyan I, Melb M, Rameshc S, Khalidd KA, Porous hydroxyapatite for artificial bone applications, Sci Tech Adv Mater, 8: 116–123, 2007.
[25]	Boskey AL, Bone composition: relationship to bone fragility and antiosteoporotic drug effects, Nature: Bone K Ey Reports, 44: 7 1-11, 2013.
[26]	Mbarki M, Sharrock P, Fiallo M, EIFeki H, Hydroxyapatite bioceramic with large porosity, Mater Sci Eng, 76: 985-990, 2017.
[27]	Liu, FH, Fabrication of Bioceramic Bone Scaffolds for Tissue Engineering, J Mater Eng Perform, 23: 3762-3769, 2014.
[28]	Greeshma T, Giridhar M, Bikramjit B, In vitro/In vivo assessment and mechanisms of toxicity of bioceramic materials and its wear particulates, RSC Adv, 4: 12763-12781, 2014.
[29]	Senthil R, Nivedita P, Hemalatha T, Vedakumari WS, Sastry TP, A possible wound dressing material from marine food waste, Int. J. Artif. Organs, 39: 509-517, 2016.
[30]	Nudelman F, Pieterse K, George A, Bomans PH, Friedrich H, Brylka LJ, Hilbers PA, de With G, Sommerdijk NA, The role of collagen in bone apatite formation in the presence of hydroxyapatite nucleation inhibitors, Nature Materials,: 1004–1009, 2013.

Cite This Article

Plain Text BibTeX RIS

APA Style

Rethinam Senthil, Sathyaraj Weslen Vedakumari, Thotapalli Parvathaleswara Sastry. (2018). Hydroxyapatite and Demineralized Bone Matrix from Marine Food Waste – A Possible Bone Implant. American Journal of Materials Synthesis and Processing, 3(1), 1-6. https://doi.org/10.11648/j.ajmsp.20180301.11

Copy | Download

ACS Style

Rethinam Senthil; Sathyaraj Weslen Vedakumari; Thotapalli Parvathaleswara Sastry. Hydroxyapatite and Demineralized Bone Matrix from Marine Food Waste – A Possible Bone Implant. Am. J. Mater. Synth. Process. 2018, 3(1), 1-6. doi: 10.11648/j.ajmsp.20180301.11

Copy | Download

AMA Style

Rethinam Senthil, Sathyaraj Weslen Vedakumari, Thotapalli Parvathaleswara Sastry. Hydroxyapatite and Demineralized Bone Matrix from Marine Food Waste – A Possible Bone Implant. Am J Mater Synth Process. 2018;3(1):1-6. doi: 10.11648/j.ajmsp.20180301.11

Copy | Download

@article{10.11648/j.ajmsp.20180301.11,
  author = {Rethinam Senthil and Sathyaraj Weslen Vedakumari and Thotapalli Parvathaleswara Sastry},
  title = {Hydroxyapatite and Demineralized Bone Matrix from Marine Food Waste – A Possible Bone Implant},
  journal = {American Journal of Materials Synthesis and Processing},
  volume = {3},
  number = {1},
  pages = {1-6},
  doi = {10.11648/j.ajmsp.20180301.11},
  url = {https://doi.org/10.11648/j.ajmsp.20180301.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajmsp.20180301.11},
  abstract = {In the present study, a novel bone implant (BI) was prepared using demineralized bone matrix (DBM) and hydroxyapatite (HA) isolated from Bluefin trevally (BT) bones, which was considered to be a marine industry food waste. Gelatin (GA) was used as a binder. Physico-chemical characterization and in vitro studies were carried out using this implant. Fourier transform infrared spectrum of BI exhibited the characteristic bands of all the three components viz., DBM, HA and GA, while scanning electron microscopic studies revealed the irregular shape of the particles. The mechanical properties of BI were also appreciable. In vitro studies were carried out using Human keratinocyte cell line (HaCaT), wherein MTT (3-(4,5-dimethylazol-2-yl)-2,5-diphenyl-tetrazolium bromide) assay proved the biocompatibility of BI. From the results obtained it could be stated that BI prepared from waste marine bones could serve as a promising biomaterial for bone tissue engineering applications.},
 year = {2018}
}

Copy | Download

TY  - JOUR
T1  - Hydroxyapatite and Demineralized Bone Matrix from Marine Food Waste – A Possible Bone Implant
AU  - Rethinam Senthil
AU  - Sathyaraj Weslen Vedakumari
AU  - Thotapalli Parvathaleswara Sastry
Y1  - 2018/01/18
PY  - 2018
N1  - https://doi.org/10.11648/j.ajmsp.20180301.11
DO  - 10.11648/j.ajmsp.20180301.11
T2  - American Journal of Materials Synthesis and Processing
JF  - American Journal of Materials Synthesis and Processing
JO  - American Journal of Materials Synthesis and Processing
SP  - 1
EP  - 6
PB  - Science Publishing Group
SN  - 2575-1530
UR  - https://doi.org/10.11648/j.ajmsp.20180301.11
AB  - In the present study, a novel bone implant (BI) was prepared using demineralized bone matrix (DBM) and hydroxyapatite (HA) isolated from Bluefin trevally (BT) bones, which was considered to be a marine industry food waste. Gelatin (GA) was used as a binder. Physico-chemical characterization and in vitro studies were carried out using this implant. Fourier transform infrared spectrum of BI exhibited the characteristic bands of all the three components viz., DBM, HA and GA, while scanning electron microscopic studies revealed the irregular shape of the particles. The mechanical properties of BI were also appreciable. In vitro studies were carried out using Human keratinocyte cell line (HaCaT), wherein MTT (3-(4,5-dimethylazol-2-yl)-2,5-diphenyl-tetrazolium bromide) assay proved the biocompatibility of BI. From the results obtained it could be stated that BI prepared from waste marine bones could serve as a promising biomaterial for bone tissue engineering applications.
VL  - 3
IS  - 1
ER  -

Copy | Download

Author Information

Rethinam Senthil

Biological Materials Lab, Central Leather Research Institute, Chennai, India
Sathyaraj Weslen Vedakumari

Faculty of Allied Health Science, Chettinad Academy of Research & Education, Chennai, India
Thotapalli Parvathaleswara Sastry

Biological Materials Lab, Central Leather Research Institute, Chennai, India

Download PDF

Sections

Plain Text BibTeX RIS

APA Style

Rethinam Senthil, Sathyaraj Weslen Vedakumari, Thotapalli Parvathaleswara Sastry. (2018). Hydroxyapatite and Demineralized Bone Matrix from Marine Food Waste – A Possible Bone Implant. American Journal of Materials Synthesis and Processing, 3(1), 1-6. https://doi.org/10.11648/j.ajmsp.20180301.11

Copy | Download

ACS Style

Rethinam Senthil; Sathyaraj Weslen Vedakumari; Thotapalli Parvathaleswara Sastry. Hydroxyapatite and Demineralized Bone Matrix from Marine Food Waste – A Possible Bone Implant. Am. J. Mater. Synth. Process. 2018, 3(1), 1-6. doi: 10.11648/j.ajmsp.20180301.11

Copy | Download

AMA Style

Rethinam Senthil, Sathyaraj Weslen Vedakumari, Thotapalli Parvathaleswara Sastry. Hydroxyapatite and Demineralized Bone Matrix from Marine Food Waste – A Possible Bone Implant. Am J Mater Synth Process. 2018;3(1):1-6. doi: 10.11648/j.ajmsp.20180301.11

Copy | Download

@article{10.11648/j.ajmsp.20180301.11,
  author = {Rethinam Senthil and Sathyaraj Weslen Vedakumari and Thotapalli Parvathaleswara Sastry},
  title = {Hydroxyapatite and Demineralized Bone Matrix from Marine Food Waste – A Possible Bone Implant},
  journal = {American Journal of Materials Synthesis and Processing},
  volume = {3},
  number = {1},
  pages = {1-6},
  doi = {10.11648/j.ajmsp.20180301.11},
  url = {https://doi.org/10.11648/j.ajmsp.20180301.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajmsp.20180301.11},
  abstract = {In the present study, a novel bone implant (BI) was prepared using demineralized bone matrix (DBM) and hydroxyapatite (HA) isolated from Bluefin trevally (BT) bones, which was considered to be a marine industry food waste. Gelatin (GA) was used as a binder. Physico-chemical characterization and in vitro studies were carried out using this implant. Fourier transform infrared spectrum of BI exhibited the characteristic bands of all the three components viz., DBM, HA and GA, while scanning electron microscopic studies revealed the irregular shape of the particles. The mechanical properties of BI were also appreciable. In vitro studies were carried out using Human keratinocyte cell line (HaCaT), wherein MTT (3-(4,5-dimethylazol-2-yl)-2,5-diphenyl-tetrazolium bromide) assay proved the biocompatibility of BI. From the results obtained it could be stated that BI prepared from waste marine bones could serve as a promising biomaterial for bone tissue engineering applications.},
 year = {2018}
}

Copy | Download

TY  - JOUR
T1  - Hydroxyapatite and Demineralized Bone Matrix from Marine Food Waste – A Possible Bone Implant
AU  - Rethinam Senthil
AU  - Sathyaraj Weslen Vedakumari
AU  - Thotapalli Parvathaleswara Sastry
Y1  - 2018/01/18
PY  - 2018
N1  - https://doi.org/10.11648/j.ajmsp.20180301.11
DO  - 10.11648/j.ajmsp.20180301.11
T2  - American Journal of Materials Synthesis and Processing
JF  - American Journal of Materials Synthesis and Processing
JO  - American Journal of Materials Synthesis and Processing
SP  - 1
EP  - 6
PB  - Science Publishing Group
SN  - 2575-1530
UR  - https://doi.org/10.11648/j.ajmsp.20180301.11
AB  - In the present study, a novel bone implant (BI) was prepared using demineralized bone matrix (DBM) and hydroxyapatite (HA) isolated from Bluefin trevally (BT) bones, which was considered to be a marine industry food waste. Gelatin (GA) was used as a binder. Physico-chemical characterization and in vitro studies were carried out using this implant. Fourier transform infrared spectrum of BI exhibited the characteristic bands of all the three components viz., DBM, HA and GA, while scanning electron microscopic studies revealed the irregular shape of the particles. The mechanical properties of BI were also appreciable. In vitro studies were carried out using Human keratinocyte cell line (HaCaT), wherein MTT (3-(4,5-dimethylazol-2-yl)-2,5-diphenyl-tetrazolium bromide) assay proved the biocompatibility of BI. From the results obtained it could be stated that BI prepared from waste marine bones could serve as a promising biomaterial for bone tissue engineering applications.
VL  - 3
IS  - 1
ER  -

Copy | Download