A well-known herbal remedy for a variety of conditions is withania sominifera. Using a combination of indole butyric acid (IBA) at 1.0 mg/l and indole acetic acid (IAA) at 0.5 mg/L, adventitious roots were successfully initiated from leaf explants of in vitro propagated plantlets in MS medium in the current study. The best conditions for inducing hairy roots were found to be a co-culture period of 24 hours with Agrobacterium rhizogenes. Within 7 days of treatment, hairy roots started to appear. The addition of 50 M acetosyringone and a 1-hour infection time was found to increase the transformation frequency from 72.11 0.5% to 81.22 0.24%. Following a 30-day period of culture, the induced roots were moved to a shake flask system, and the growth index was discovered to be 33.35 for adventitious roots and 37.24 for hairy roots. The induced roots were moved to a shake flask culture system for a 30-day period of culture, and the growth indices for adventitious roots and hairy roots were found to be 33.35 and 37.24, respectively. The roots were extracted after being collected, and they were then screened for phytochemicals using GC-MS, TLC, and HPLC. Alkaloids, flavanoids, tannins, saponins, phenols, glycosides, terpenoids, reducing sugar, and anthraquinones were found in these analyses. The presence of Withaferin A and Withanolide A was confirmed by HPLC of the methanolic extracts of the roots. Withaferin A was present in transformed roots at a concentration of 69.21 g/g dry weight, which was two times greater than that of adventitious root cultures at 32.45 g/g dry weight. This work emphasises the importance of Withania sominifera poshita variety adventitious and hairy root culture, which may be utilised for systematic and reliable metabolite production that can be used for scientific and commercial applications.
| Published in | American Journal of Plant Biology (Volume 8, Issue 2) |
| DOI | 10.11648/j.ajpb.20230802.13 |
| Page(s) | 36-42 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2023. Published by Science Publishing Group |
Withania sominifera Poshita Variety, Adventitious Roots, Hairy Roots
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APA Style
Viji Mo, Neeba Wilson. (2023). Enhanced Production of Secondary Metabolites Through Adventitious and Hairy Root Cultures of Withania sominifera cv. Poshita. American Journal of Plant Biology, 8(2), 36-42. https://doi.org/10.11648/j.ajpb.20230802.13
ACS Style
Viji Mo; Neeba Wilson. Enhanced Production of Secondary Metabolites Through Adventitious and Hairy Root Cultures of Withania sominifera cv. Poshita. Am. J. Plant Biol. 2023, 8(2), 36-42. doi: 10.11648/j.ajpb.20230802.13
AMA Style
Viji Mo, Neeba Wilson. Enhanced Production of Secondary Metabolites Through Adventitious and Hairy Root Cultures of Withania sominifera cv. Poshita. Am J Plant Biol. 2023;8(2):36-42. doi: 10.11648/j.ajpb.20230802.13
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Download@article{10.11648/j.ajpb.20230802.13,
author = {Viji Mo and Neeba Wilson},
title = {Enhanced Production of Secondary Metabolites Through Adventitious and Hairy Root Cultures of Withania sominifera cv. Poshita},
journal = {American Journal of Plant Biology},
volume = {8},
number = {2},
pages = {36-42},
doi = {10.11648/j.ajpb.20230802.13},
url = {https://doi.org/10.11648/j.ajpb.20230802.13},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajpb.20230802.13},
abstract = {A well-known herbal remedy for a variety of conditions is withania sominifera. Using a combination of indole butyric acid (IBA) at 1.0 mg/l and indole acetic acid (IAA) at 0.5 mg/L, adventitious roots were successfully initiated from leaf explants of in vitro propagated plantlets in MS medium in the current study. The best conditions for inducing hairy roots were found to be a co-culture period of 24 hours with Agrobacterium rhizogenes. Within 7 days of treatment, hairy roots started to appear. The addition of 50 M acetosyringone and a 1-hour infection time was found to increase the transformation frequency from 72.11 0.5% to 81.22 0.24%. Following a 30-day period of culture, the induced roots were moved to a shake flask system, and the growth index was discovered to be 33.35 for adventitious roots and 37.24 for hairy roots. The induced roots were moved to a shake flask culture system for a 30-day period of culture, and the growth indices for adventitious roots and hairy roots were found to be 33.35 and 37.24, respectively. The roots were extracted after being collected, and they were then screened for phytochemicals using GC-MS, TLC, and HPLC. Alkaloids, flavanoids, tannins, saponins, phenols, glycosides, terpenoids, reducing sugar, and anthraquinones were found in these analyses. The presence of Withaferin A and Withanolide A was confirmed by HPLC of the methanolic extracts of the roots. Withaferin A was present in transformed roots at a concentration of 69.21 g/g dry weight, which was two times greater than that of adventitious root cultures at 32.45 g/g dry weight. This work emphasises the importance of Withania sominifera poshita variety adventitious and hairy root culture, which may be utilised for systematic and reliable metabolite production that can be used for scientific and commercial applications.},
year = {2023}
}
TY - JOUR T1 - Enhanced Production of Secondary Metabolites Through Adventitious and Hairy Root Cultures of Withania sominifera cv. Poshita AU - Viji Mo AU - Neeba Wilson Y1 - 2023/07/06 PY - 2023 N1 - https://doi.org/10.11648/j.ajpb.20230802.13 DO - 10.11648/j.ajpb.20230802.13 T2 - American Journal of Plant Biology JF - American Journal of Plant Biology JO - American Journal of Plant Biology SP - 36 EP - 42 PB - Science Publishing Group SN - 2578-8337 UR - https://doi.org/10.11648/j.ajpb.20230802.13 AB - A well-known herbal remedy for a variety of conditions is withania sominifera. Using a combination of indole butyric acid (IBA) at 1.0 mg/l and indole acetic acid (IAA) at 0.5 mg/L, adventitious roots were successfully initiated from leaf explants of in vitro propagated plantlets in MS medium in the current study. The best conditions for inducing hairy roots were found to be a co-culture period of 24 hours with Agrobacterium rhizogenes. Within 7 days of treatment, hairy roots started to appear. The addition of 50 M acetosyringone and a 1-hour infection time was found to increase the transformation frequency from 72.11 0.5% to 81.22 0.24%. Following a 30-day period of culture, the induced roots were moved to a shake flask system, and the growth index was discovered to be 33.35 for adventitious roots and 37.24 for hairy roots. The induced roots were moved to a shake flask culture system for a 30-day period of culture, and the growth indices for adventitious roots and hairy roots were found to be 33.35 and 37.24, respectively. The roots were extracted after being collected, and they were then screened for phytochemicals using GC-MS, TLC, and HPLC. Alkaloids, flavanoids, tannins, saponins, phenols, glycosides, terpenoids, reducing sugar, and anthraquinones were found in these analyses. The presence of Withaferin A and Withanolide A was confirmed by HPLC of the methanolic extracts of the roots. Withaferin A was present in transformed roots at a concentration of 69.21 g/g dry weight, which was two times greater than that of adventitious root cultures at 32.45 g/g dry weight. This work emphasises the importance of Withania sominifera poshita variety adventitious and hairy root culture, which may be utilised for systematic and reliable metabolite production that can be used for scientific and commercial applications. VL - 8 IS - 2 ER -
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