Manipulation of Fertility in Crossbred Dairy Cattle Using Controlled Internal Drug Release Device Combined With Prostaglandin and Equine Chorionic Gonadotrophin

Alemselam Birhanu Mekonnin; A. Forbes Howie; Christopher R. Harlow; Goitom Gidey; Desalew Tadesse Tegegne; Gidena Desta; Tadesse Gugsa; Berihu Gebrekidan; Gebregiorgis Ashebir; Simon C. Riley

doi:doi:10.11648/j.avs.20160403.12

Manipulation of Fertility in Crossbred Dairy Cattle Using Controlled Internal Drug Release Device Combined With Prostaglandin and Equine Chorionic Gonadotrophin

Alemselam Birhanu Mekonnin, A. Forbes Howie, Christopher R. Harlow, Goitom Gidey, Desalew Tadesse Tegegne, Gidena Desta, Tadesse Gugsa, Berihu Gebrekidan, Gebregiorgis Ashebir, Simon C. Riley

Published in Animal and Veterinary Sciences (Volume 4, Issue 3)

Received: 13 December 2015 Accepted: 27 April 2016 Published: 23 May 2016

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Abstract

Simplifying and improving our understanding of the protocols for inducing or synchronizing estrus is important for improving management of dairy cattle. This study evaluated the estrus response and conception rate of crossbred (Holstein Friesian X Zebu) dairy cows (n=75) and heifers (n=47) assigned to a 10-day controlled internal drug release (CIDR) device – prostaglandin F2-alpha (PGF2α) – equine chorionic gonadotrophin (eCG) based estrus synchronization protocol. Animals were assigned to three groups (a) anestrus (n=62), (b) repeat-breeders (n=11) and (c) silent estrus (n=46), according to their reproductive history, per rectal palpation, and progesterone concentrations determined by On-Farm enzyme-linked immunosorbent assays (ELISA) in milk (lactating cows) or serum (dry cows and heifers). For each animal, a CIDR device (containing 1.38g progesterone) was inserted into the vagina and allowed to remain in-situ for 10 days. On Day 8 after device insertion, a dose of 500µg of PGF2α was administered intramuscularly (IM). On Day 10 (device removal), 500 IU of eCG was given IM. Animals were inseminated or mated 48 and 72 hrs post device removal. Pregnancy was assessed 20-24 days post insemination by measurement of progesterone in milk/serum and pregnancy was confirmed by per rectal palpation and/or ultrasonography 44-90 days post insemination. Overall estrus response and conception rates were 97.5% and 78.3%, respectively, with no significant differences in parity, pre-treatment reproductive status and farming system (smallholder vs organized commercial farms). Conception rate after induced/synchronized estrus was 82.9% and 72.1% in cows and heifers, respectively, with an overall calving rate of 94.4%. In conclusion, the present study has shown high estrus response and conception rate in crossbred dairy cattle using this CIDR - PGF2α - eCG based estrus synchronization protocol. Application of this protocol is highly recommended to enhance fertility of dairy cattle in the study area, and this could readily be applied to other regions.

Published in	Animal and Veterinary Sciences (Volume 4, Issue 3)
DOI	10.11648/j.avs.20160403.12
Page(s)	39-46
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2016. Published by Science Publishing Group

Keywords

Cattle, CIDR, eCG, Estrus, Fertility, PGF2α, Synchronization

References

[1]	Kumar, N. and Tkui, K. (2014). Reproductive performance of crossbred dairy cows in Mekelle, Ethiopia. Sci. J. Anim. Sci. 3(2): 35-40.
[2]	Kumar, N., Abadi, Y., Gebrekidan, B. and Woldearegay, Y. H. (2014). Productive and Reproductive Performance of Local Cows under Farmer’s Management in and around Mekelle, Ethiopia. IOSR-JAVS 7(5): 21-24.
[3]	Dickson, N., Siew N., Singh-Knights, D., Bourne G. and Knights, M. (2012). Technical and Economic Outcome Using Various Timed Artificial Insemination Protocols in Dairy Cattle in the Tropics. J. Anim. Prod. Adv. 2(1): 490-499.
[4]	Perez-Marin, C., Moreno, M. and Calero, G. (2012). Clinical approach to the repeat breeder cow syndrome. (Ed. Perez-Marin C). A Bird's-Eye View of Veterinary Medicine, 337-362.
[5]	Dubuc, J. (2011). Postpartum Uterine Diseases: Prevalence, Impacts, and Treatments. WCDS Advances in Dairy Technology. 23: 255-267.
[6]	Mekonnin, A. B., Harlow, C. R., Gidey, G., Tadesse, D., Desta, G., Gugssa, T. and Riley, S. C. (2015a). Assessment of Reproductive Performance and Problems in Crossbred (Holstein Friesian X Zebu) Dairy Cattle in and around Mekelle, Tigray, Ethiopia. J. Anim. Vet. 3(3): 94-101.
[7]	Shiferaw, Y., Tenhagen, B. A., Bekana, M. and Kassa. T. (2003). Reproductive performance of crossbredd cows in different production systems in the central highlands of Ethiopia. Trop. Anim. Health and Prod. 35: 551-561.
[8]	Duguma, B., Kechero, Y. and Janssens, G-P. J. (2012). Productive and Reproductive Performance of Zebu X Holstein-Friesian Crossbred Dairy Cows in Jimma Town, Oromia, Ethiopia. Global Veterinaria 8(1): 67-72.
[9]	Walker, W. L., Nebel, R. L. and McGillard, M. L. (1996). Time of ovulation relative to mounting activity in dairy cattle. J. Dairy Sci. 79: 1555-1561.
[10]	Hansar, E., Lemma, A. and Yilma, T. (2014). Pre-service ultrasonic and manual evaluation of the reproductive organs of dairy cows presumed to be in estrus. Springer Plus 3: 529.
[11]	Nebel, R. and DeJarnette, M. (2015). Dairy cow synchronization protocols. Select Sirs. Available at: http://www.selectsires.com/resources/fertilitydocs/dairy_cow_synch_protocols.pdf. (Accessed 20 September 2015).
[12]	Larson, J. E., Lamb, G. C., Stevenson, J. S., Johnson, S. K., Day, M. L., Geary, T. W., Kesler, D. J., DeJarnette, J. M., Schrick, F. N., DiCostanzo, A. and Arseneau, J. D. (2006). Synchronization of estrus in suckled beef cows for detected estrus and artificial insemination and timed artificial insemination using gonadotropin-releasing hormone, prostaglandin F, and progesterone. J. Anim. Sci. 84: 332-342.
[13]	Lamb, G. C, Dahlen, C. R., Larson, J. E., Marquezini, G. and Stevenson, J. S. (2010). Control of the estrous cycle to improve fertility for fixed-time artificial insemination in beef cattle: a review. J. Anim. Sci. 88(13 Suppl): E181-192.
[14]	Patterson, D. J. Mallory, D. A. Nash, J. M. Martin, N. T. and Smith, M. F. (2011). Strategies to optimize use of AI in cow/calf production systems: focus on fixed-time AI protocols for cows Proceedings, Applied Reproductive Strategies in Beef Cattle – Northwest, September 30 - October 1, 2011; Boise, ID, USA.
[15]	Williams, G., Stanko, R., Allen, C., Cardoso, R., Prezotto, L., Thorson, J., and Amstalden. M. (2011). Evidence that prostaglandin administration at the onset of a 5-day CO-Synch + CIDR synchronization protocol markedly improves fixed-time AI pregnancy rates in Bos indicus-influenced cattle. J. Anim. Sci. 89 (E-Suppl. 1): 264.
[16]	Siregar, T. N., Hamdan, H., Riady, G., Panjaitan, B., Aliza, D., Pratiwi, E. F., Darianto T., and Husnurrizal (2015). Efficacy of Two Estrus Synchronization Methods in Indonesian Aceh Cattle. Inter. J. Vet. Sci. 4(2): 87-91.
[17]	DeJarnette, M. (2015). Ovsynch, Co-synch, Presynch and Kitchensynch: how did breeding cows get so complicated? Select Sires. pp. 1-6. Available at: http://www.selectsires.com (Accessed 2 September 2015).
[18]	Yelich, J. V. and Bridges, G. A. (2012). Synchronization response: Bos taurus vs. Bos indicus cattle. A review. Beef Improvement Federation (BIF) Research Symposium and Annual Meeting, April 18-21, 2012, Houston, Texas, USA.
[19]	Powell, J. G., Lester, T. D., Reynolds, J. L., Davis, A. J., Hornsby, J. A. and Rorie, R. W. (2012). Comparison of a 5- or 14-day CIDR-based estrous synchronization protocols with sorted semen in beef heifers. AAES Research Series 606. pp. 51-53.
[20]	Maurer, R. E., Webel, S. K. and Brown, M. D. (1975). Ovulation control in cattle with progesterone intravaginal device (PRID) and gonadotropin releasing hormone (GnRH). Ann. Biol. Anim. Bioch. Biophys. 15: 291-296.
[21]	Macmillan, K. L. and Peterson, A. J., (1993). A new intravaginal progesterone releasing device for cattle (CIDR-B) for oestrous synchronization, increasing pregnancy rates and the treatment of postpartum anoestrus. Anim. Reprod. Sci. 33: 1-25.
[22]	Gordon, I. (1999). Artificial control of oestrus and ovulation. In: I. Gordon (ed.) Controlled reproduction in cattle & buffaloes. CAB Int. Publ., Wallingford, UK, pp. 133-166.
[23]	Palomares, R. A., Fishman, H. J., Jones, A. L., Ferrer, M. S., Jenerette, M. and Vaughn A. (2015). Comparison of 4- versus 5-day Co-Synch þ controlled internal drug release (CIDR) þ timed artificial insemination protocols in dairy heifers. Theriogenology 84: 868-874.
[24]	Bridges, G. A., Helser, L. A., Grum, D. E., Mussard, M. L., Gasser, C. L. and Day, M. L. (2008). Decreasing the interval between GnRH and PGF2alpha from 7 to 5 days and lengthening proestrus increases timed-AI pregnancy rates in beef cows. Theriogenology 15: 843-851.
[25]	Broadbent, P. J., Tregaskes, L. D., Dolman, D. F., Franklin, M. F. and Jones, R. L. (1993). Synchronlzatlon of estrus in embryo transfer recipients after using a combination of PRID or CIDR-B plus PGF2α. Theriogenology 39: 1055-1065.
[26]	Kim, U. H., Suh, G. H., Hur, T. Y., Kang, S. J., Kang, H. G., Park, S. B., Kim, S. H. and Kim, I. H. (2007). Comparison of two types of CIDR-based timed artificial insemination protocols for repeat breeder dairy cows. J. Reprod. And Develop. 53: 639-645.
[27]	Bridges, G. A., Lake, S. L., Kruse, S. G., Bird, S. L., Funnell, B. J., Aries, R., Walker, J. A., Grant, J. K. and Perry, G. A. (2014). Comparison of three CIDR-based fixed-time AI protocols in beef heifers. J. Anim. Sci. 92: 3127-3133.
[28]	Romano, J. E. and Fahning, M. L. (2013). Comparison between 7 vs 9 days of controlled internal drug release inserts permanency on oestrus performance and fertility in lactating dairy cattle. Ital. J. Anim. Sci. 12(e63): 390-394.
[29]	Hailu, B., Gebrekidan, B., Raju, S., Birhanu, A. and Tadesse, G. (2015). Effects of Gonadotropin Releasing Hormone Analogue in Enhancements of Pregnancy in Repeat Breeding Dairy Cows in and around Mekelle, Tigray, Ethiopia. Anim. Vet. Sci. 3(1): 12-17.
[30]	Mekonnin, A. B. Harlow, C., Howie, F., Gidey, G., Tadesse, D., Desta, G., Ashebir, G., Gebrekidan, B., Gugsa, T. and Riley, S. C. (2015b). Assessment of major reproductive problems and reproductive status of crossbred (Holstein Friesian X Zebu) dairy cattle in and around Mekelle, Tigray, Ethiopia. Society for Reproduction and Fertility annual conference, 19-22 July 2015, Oxford, UK.
[31]	BoPED (2011). Bureau of Planning and Economic Development: Tigray, Ethiopia.
[32]	DEFRA (2001). Condition scoring of dairy cows: Action on animal health and welfare. The Department for Environment, Food and Rural Affairs, UK.
[33]	Kawate, N., Watanabe, K., Urnaka, K., Takahashi, M., Inaba, T. and Tamada, H. (2011). Comparison of plasma concentration of estradiol 17β and progesterone, and conception in dairy cows with cystic ovarian diseases between Ovsynch and Ovsynch plus CIDR timed protocols. J. Reprod. Develop. 57: 267-272.
[34]	Lucy, M. C., Billings, H. J. Butler, W. R. Ehnes, L. R. Fields, M. J. Kesler, D. J. Kinder, J. E. Mattos, R. C. Short, R. E. Thatcher, W. W. Wettemann, R. P. Yelich, J. V. and Hafs. H. D. (2001). Efficacy of an Intravaginal Progesterone Insert and an Injection of PGF2a Synchronizing Estrus and Shortening the Interval to Pregnancy in Postpartum Beef cows, Peripubertal Beef Heifers, and Dairy Heifers. J. Anim. Sci. 79: 982-995.
[35]	EBLEX, (2014). Artificial insemination (AI) and oestrus synchronisation of beef cattle. Better Returns Program (BRP). pp. 1-12. http://beefandlamb.ahdb.org.uk/wp/wp-content/uploads/2014/07/BRP-plus-artificial-insemination090714.pdf. (Accessed 12 September 2015).
[36]	Racowsky, C. (1991). Gamete resources: origin and production of oocytes. (Eds. Pedersen RA., McLaren A, First, NL). Animal applications of research in mammalian development. Current communications in cell and molecular biology 4, 26-38, Cold Spring Harbor Laboratory Press, New York, USA.
[37]	Gospodarowicz, D. (1972). Purification and physiochemical properties of pregnant mare serum gonadotropin (PMSG). Endocrinology 91: 101-106.
[38]	Sá Filho, O. G, Meneghetti, M., Peres, R. F., Lamb, G. C. and Vasconcelos, J. L. (2009). Fixed-time artificial insemination with estradiol and progesterone for Bos indicus cows II: strategies and factors affecting fertility. Theriogenology 72: 210-218.
[39]	Tegegne, A., Franceschini, R. and Sovani, S. (1994). Superovulatory response, embryo recovery and progesterone secretion in Boran (Full-size image (<1 K)) cows after treatment with either Pergovet or Pluset. Theriogenology 41(8): 1653-1662.
[40]	Grant, E. (2006). Understanding CIDR. National Association of Animal Breeders. Accessed at: http://beefmagazine.com/mag/beef_understanding_cidr (Accessed 10 September 2015).
[41]	Garcia-Winder, M., Lewis, P. E., Deaver, D. R., Smith, V. G., Lewis, G. S. and Inskeep, E. K. (1986). Endocrine profiles associated with life span of induced corpora lutea in postpartum beef cows. J. Anim. Sci. 62: 1353-1362.
[42]	Rosenberg, M., Kaim, M., Herz, Z. and Folman, Y. (1990). Comparisons of methods for synchronization of estrous cycle in dairy cows. 1. Effects on plasma progesterone and manifestation of estrus. J. Dairy Sci. 73(10): 2807-2816.
[43]	Fike, K. E., Day, M. L, Inskeep, E. K., Kinder, J. E., Lewis, P. E., Short, R. E. and Hafs, H. D. (1997). Estrus and luteal function in suckled beef cows that were anestrous when treated with an intravaginal device containing progesterone with or without a subsequent injection of estradiol benzoate. J. Anim. Sci. 75(8): 2009-2015.
[44]	McDougall, S., Burke, C. R., Macmillan, K. L. and Williamson, N. B. (1992). The effect of pretreatment with progesterone on the oestrous response to oestradiol-17β benzoate in the post-partum dairy cow. Proc. N.Z. Soc. Anim. Prod. 52: 157-160.
[45]	Gasser, C. L. (2013). Joint Alphapharma – Beef Species Symposium, considerations on puberty in replacement beef heifers. J. Anim. Sci. 91: 1336-1340.
[46]	McNally, J. C., Crowe, M. A., Roche, J. F., Beltman, M. E. (2014). Effects of physiological and/or disease status on the response of postpartum dairy cows to synchronization of estrus using an intravaginal progesterone device. Theriogenology 82(9): 1263-1272.
[47]	Singh, H., Luthra, R. A., Khar, S. K. and Nanda, T. (2006). Oestrus Induction, Plasma Steroid Hormone Profiles and Fertility Response after CIDR and eCG Treatment in Acyclic Sahiwal Cows. Asian-Aust. J. Anim. Sci. 19(11): 1566-1573.
[48]	Virmani, M., Malik, R. K., Dalal, D. S., Singh, P. and Vikash (2013). Comparing efficacy of different hormonal protocols for induction of cyclicity in postpartum anestrus Sahiwal cows. Haryana Veterinarian 52: 121-123.
[49]	Kasimanickam, R., Schroeder, S., Hall, J. B. and Whittier, W. D. (2015). Fertility after implementation of long- and short-term progesterone-based ovulation synchronization protocols for fixed-time artificial insemination in beef heifers. Theriogenology 83: 1226-1232.
[50]	Gordon, I. (1996). Controlled reproduction in cattle and buffaloes. CAB International. Wallingford, UK.
[51]	Lamb, G. C., Stevenson, J. S. Kesler, D. J. Garverick, H. A. Brown, D. R. and Salfen, B. E. (2001). Inclusion of an intravaginal progesterone insert plus GnRH and prostaglandin F2α for ovulation control in postpartum suckled beef cows. J. Anim. Sci. 79: 2253-2259.
[52]	Colazo M. G., Kastelic, J. P, Whittaker, P. R., Gavaga, Q. A., Wilde, R., Mapletoft, R. J. (2004). Fertility in beef cattle given a new or previously used CIDR insert and estradiol, with or without progesterone. Anim. Reprod. Sci. 81: 25-34.
[53]	Smith, M. W. and Stevenson. J. S. (1995). Fate of the dominant follicle, embryonal survival, and pregnancy rates in dairy cattle treated with prostaglandin F2α and progestins in the absence or presence of a functional corpus luteum. J. Anim. Sci. 73: 3743-3751.
[54]	Zobel, R., Rkalcic, S., Pipal, I. and Buic, V. (2011). Incidence and factors associated with early pregnancy losses in Simmental dairy cows. Anim. Reprod. Sci. 127: 121-125.
[55]	Mekonnin, A. B, Tadesse, G., Bitsue, H. K. and Khar, S. K. (2016). Efficacy of a Modified GnRH-PGF Combination for 2 Estrous Synchronization in Dairy Cattle. Global Veterinaria 16(2): 200-205.
[56]	Giday, J. (2014). Livestock development in Tigray, an overview of past and current development efforts. Livestock Development and Animal Health Services Main Department, Tigray BoARD, Mekelle, Tigray, Ethiopia. A report presented at International Conference on Enhancing Economic Growth and Public Health through Livestock Development and One Health Approach (Mekelle, Ethiopia).
[57]	Lobago, F. (2007). Reproductive and lactation performance of dairy cattle in the Oromia Central Highlands of Ethiopia with special emphasis on pregnancy period. Doctoral thesis Swedish University of Agricultural Sciences, Uppsala.
[58]	Ashebir, G., Birhanu A. and Gugsa, T. (2016). Status of artificial insemination in Tigray Regional State, “Constraints and acceptability under field condition”. J. Dairy Vet. Anim. Res. 3(3): 00078. DOI: 10.15406/jdvar.2016.03.00078.
[59]	Stevenson, J. S., Call, E. P. Scoby, R. K. and Phatak, A. P. (1990). Double insemination and gonadotropin releasing hormone treatment of repeat-breeding dairy cattle. J. Dairy Sci. 73: 1766.
[60]	Abdallah, H. and Abd El Rahim, A. (2014). Efficiency of previously used CIDR stored for a prolonged period. Adv. Anim. Vet. Sci. 2(9): 508-515.

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Alemselam Birhanu Mekonnin, A. Forbes Howie, Christopher R. Harlow, Goitom Gidey, Desalew Tadesse Tegegne, et al. (2016). Manipulation of Fertility in Crossbred Dairy Cattle Using Controlled Internal Drug Release Device Combined With Prostaglandin and Equine Chorionic Gonadotrophin. Animal and Veterinary Sciences, 4(3), 39-46. https://doi.org/10.11648/j.avs.20160403.12

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Alemselam Birhanu Mekonnin; A. Forbes Howie; Christopher R. Harlow; Goitom Gidey; Desalew Tadesse Tegegne, et al. Manipulation of Fertility in Crossbred Dairy Cattle Using Controlled Internal Drug Release Device Combined With Prostaglandin and Equine Chorionic Gonadotrophin. Anim. Vet. Sci. 2016, 4(3), 39-46. doi: 10.11648/j.avs.20160403.12

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Alemselam Birhanu Mekonnin, A. Forbes Howie, Christopher R. Harlow, Goitom Gidey, Desalew Tadesse Tegegne, et al. Manipulation of Fertility in Crossbred Dairy Cattle Using Controlled Internal Drug Release Device Combined With Prostaglandin and Equine Chorionic Gonadotrophin. Anim Vet Sci. 2016;4(3):39-46. doi: 10.11648/j.avs.20160403.12

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@article{10.11648/j.avs.20160403.12,
  author = {Alemselam Birhanu Mekonnin and A. Forbes Howie and Christopher R. Harlow and Goitom Gidey and Desalew Tadesse Tegegne and Gidena Desta and Tadesse Gugsa and Berihu Gebrekidan and Gebregiorgis Ashebir and Simon C. Riley},
  title = {Manipulation of Fertility in Crossbred Dairy Cattle Using Controlled Internal Drug Release Device Combined With Prostaglandin and Equine Chorionic Gonadotrophin},
  journal = {Animal and Veterinary Sciences},
  volume = {4},
  number = {3},
  pages = {39-46},
  doi = {10.11648/j.avs.20160403.12},
  url = {https://doi.org/10.11648/j.avs.20160403.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.avs.20160403.12},
  abstract = {Simplifying and improving our understanding of the protocols for inducing or synchronizing estrus is important for improving management of dairy cattle. This study evaluated the estrus response and conception rate of crossbred (Holstein Friesian X Zebu) dairy cows (n=75) and heifers (n=47) assigned to a 10-day controlled internal drug release (CIDR) device – prostaglandin F2-alpha (PGF2α) – equine chorionic gonadotrophin (eCG) based estrus synchronization protocol. Animals were assigned to three groups (a) anestrus (n=62), (b) repeat-breeders (n=11) and (c) silent estrus (n=46), according to their reproductive history, per rectal palpation, and progesterone concentrations determined by On-Farm enzyme-linked immunosorbent assays (ELISA) in milk (lactating cows) or serum (dry cows and heifers). For each animal, a CIDR device (containing 1.38g progesterone) was inserted into the vagina and allowed to remain in-situ for 10 days. On Day 8 after device insertion, a dose of 500µg of PGF2α was administered intramuscularly (IM). On Day 10 (device removal), 500 IU of eCG was given IM. Animals were inseminated or mated 48 and 72 hrs post device removal. Pregnancy was assessed 20-24 days post insemination by measurement of progesterone in milk/serum and pregnancy was confirmed by per rectal palpation and/or ultrasonography 44-90 days post insemination. Overall estrus response and conception rates were 97.5% and 78.3%, respectively, with no significant differences in parity, pre-treatment reproductive status and farming system (smallholder vs organized commercial farms). Conception rate after induced/synchronized estrus was 82.9% and 72.1% in cows and heifers, respectively, with an overall calving rate of 94.4%. In conclusion, the present study has shown high estrus response and conception rate in crossbred dairy cattle using this CIDR - PGF2α - eCG based estrus synchronization protocol. Application of this protocol is highly recommended to enhance fertility of dairy cattle in the study area, and this could readily be applied to other regions.},
 year = {2016}
}

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TY  - JOUR
T1  - Manipulation of Fertility in Crossbred Dairy Cattle Using Controlled Internal Drug Release Device Combined With Prostaglandin and Equine Chorionic Gonadotrophin
AU  - Alemselam Birhanu Mekonnin
AU  - A. Forbes Howie
AU  - Christopher R. Harlow
AU  - Goitom Gidey
AU  - Desalew Tadesse Tegegne
AU  - Gidena Desta
AU  - Tadesse Gugsa
AU  - Berihu Gebrekidan
AU  - Gebregiorgis Ashebir
AU  - Simon C. Riley
Y1  - 2016/05/23
PY  - 2016
N1  - https://doi.org/10.11648/j.avs.20160403.12
DO  - 10.11648/j.avs.20160403.12
T2  - Animal and Veterinary Sciences
JF  - Animal and Veterinary Sciences
JO  - Animal and Veterinary Sciences
SP  - 39
EP  - 46
PB  - Science Publishing Group
SN  - 2328-5850
UR  - https://doi.org/10.11648/j.avs.20160403.12
AB  - Simplifying and improving our understanding of the protocols for inducing or synchronizing estrus is important for improving management of dairy cattle. This study evaluated the estrus response and conception rate of crossbred (Holstein Friesian X Zebu) dairy cows (n=75) and heifers (n=47) assigned to a 10-day controlled internal drug release (CIDR) device – prostaglandin F2-alpha (PGF2α) – equine chorionic gonadotrophin (eCG) based estrus synchronization protocol. Animals were assigned to three groups (a) anestrus (n=62), (b) repeat-breeders (n=11) and (c) silent estrus (n=46), according to their reproductive history, per rectal palpation, and progesterone concentrations determined by On-Farm enzyme-linked immunosorbent assays (ELISA) in milk (lactating cows) or serum (dry cows and heifers). For each animal, a CIDR device (containing 1.38g progesterone) was inserted into the vagina and allowed to remain in-situ for 10 days. On Day 8 after device insertion, a dose of 500µg of PGF2α was administered intramuscularly (IM). On Day 10 (device removal), 500 IU of eCG was given IM. Animals were inseminated or mated 48 and 72 hrs post device removal. Pregnancy was assessed 20-24 days post insemination by measurement of progesterone in milk/serum and pregnancy was confirmed by per rectal palpation and/or ultrasonography 44-90 days post insemination. Overall estrus response and conception rates were 97.5% and 78.3%, respectively, with no significant differences in parity, pre-treatment reproductive status and farming system (smallholder vs organized commercial farms). Conception rate after induced/synchronized estrus was 82.9% and 72.1% in cows and heifers, respectively, with an overall calving rate of 94.4%. In conclusion, the present study has shown high estrus response and conception rate in crossbred dairy cattle using this CIDR - PGF2α - eCG based estrus synchronization protocol. Application of this protocol is highly recommended to enhance fertility of dairy cattle in the study area, and this could readily be applied to other regions.
VL  - 4
IS  - 3
ER  -

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Author Information

Alemselam Birhanu Mekonnin

MRC Centre for Reproductive Health, The Queen’s Medical Research Institute College of Medicine and Veterinary Medicine, University of Edinburgh, Edinburgh, United Kingdom
A. Forbes Howie

MRC Centre for Reproductive Health, The Queen’s Medical Research Institute College of Medicine and Veterinary Medicine, University of Edinburgh, Edinburgh, United Kingdom
Christopher R. Harlow

MRC Centre for Reproductive Health, The Queen’s Medical Research Institute College of Medicine and Veterinary Medicine, University of Edinburgh, Edinburgh, United Kingdom
Goitom Gidey

College of Veterinary Medicine, Mekelle University, Mekelle, Ethiopia
Desalew Tadesse Tegegne

College of Veterinary Medicine, Mekelle University, Mekelle, Ethiopia
Gidena Desta

College of Veterinary Medicine, Mekelle University, Mekelle, Ethiopia
Tadesse Gugsa

Tigray Regional State Agriculture Bureau, Tigray, Mekelle, Ethiopia
Berihu Gebrekidan

College of Veterinary Medicine, Mekelle University, Mekelle, Ethiopia
Gebregiorgis Ashebir

College of Veterinary Medicine, Mekelle University, Mekelle, Ethiopia
Simon C. Riley

MRC Centre for Reproductive Health, The Queen’s Medical Research Institute College of Medicine and Veterinary Medicine, University of Edinburgh, Edinburgh, United Kingdom

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Alemselam Birhanu Mekonnin, A. Forbes Howie, Christopher R. Harlow, Goitom Gidey, Desalew Tadesse Tegegne, et al. (2016). Manipulation of Fertility in Crossbred Dairy Cattle Using Controlled Internal Drug Release Device Combined With Prostaglandin and Equine Chorionic Gonadotrophin. Animal and Veterinary Sciences, 4(3), 39-46. https://doi.org/10.11648/j.avs.20160403.12

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ACS Style

Alemselam Birhanu Mekonnin; A. Forbes Howie; Christopher R. Harlow; Goitom Gidey; Desalew Tadesse Tegegne, et al. Manipulation of Fertility in Crossbred Dairy Cattle Using Controlled Internal Drug Release Device Combined With Prostaglandin and Equine Chorionic Gonadotrophin. Anim. Vet. Sci. 2016, 4(3), 39-46. doi: 10.11648/j.avs.20160403.12

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AMA Style

Alemselam Birhanu Mekonnin, A. Forbes Howie, Christopher R. Harlow, Goitom Gidey, Desalew Tadesse Tegegne, et al. Manipulation of Fertility in Crossbred Dairy Cattle Using Controlled Internal Drug Release Device Combined With Prostaglandin and Equine Chorionic Gonadotrophin. Anim Vet Sci. 2016;4(3):39-46. doi: 10.11648/j.avs.20160403.12

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@article{10.11648/j.avs.20160403.12,
  author = {Alemselam Birhanu Mekonnin and A. Forbes Howie and Christopher R. Harlow and Goitom Gidey and Desalew Tadesse Tegegne and Gidena Desta and Tadesse Gugsa and Berihu Gebrekidan and Gebregiorgis Ashebir and Simon C. Riley},
  title = {Manipulation of Fertility in Crossbred Dairy Cattle Using Controlled Internal Drug Release Device Combined With Prostaglandin and Equine Chorionic Gonadotrophin},
  journal = {Animal and Veterinary Sciences},
  volume = {4},
  number = {3},
  pages = {39-46},
  doi = {10.11648/j.avs.20160403.12},
  url = {https://doi.org/10.11648/j.avs.20160403.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.avs.20160403.12},
  abstract = {Simplifying and improving our understanding of the protocols for inducing or synchronizing estrus is important for improving management of dairy cattle. This study evaluated the estrus response and conception rate of crossbred (Holstein Friesian X Zebu) dairy cows (n=75) and heifers (n=47) assigned to a 10-day controlled internal drug release (CIDR) device – prostaglandin F2-alpha (PGF2α) – equine chorionic gonadotrophin (eCG) based estrus synchronization protocol. Animals were assigned to three groups (a) anestrus (n=62), (b) repeat-breeders (n=11) and (c) silent estrus (n=46), according to their reproductive history, per rectal palpation, and progesterone concentrations determined by On-Farm enzyme-linked immunosorbent assays (ELISA) in milk (lactating cows) or serum (dry cows and heifers). For each animal, a CIDR device (containing 1.38g progesterone) was inserted into the vagina and allowed to remain in-situ for 10 days. On Day 8 after device insertion, a dose of 500µg of PGF2α was administered intramuscularly (IM). On Day 10 (device removal), 500 IU of eCG was given IM. Animals were inseminated or mated 48 and 72 hrs post device removal. Pregnancy was assessed 20-24 days post insemination by measurement of progesterone in milk/serum and pregnancy was confirmed by per rectal palpation and/or ultrasonography 44-90 days post insemination. Overall estrus response and conception rates were 97.5% and 78.3%, respectively, with no significant differences in parity, pre-treatment reproductive status and farming system (smallholder vs organized commercial farms). Conception rate after induced/synchronized estrus was 82.9% and 72.1% in cows and heifers, respectively, with an overall calving rate of 94.4%. In conclusion, the present study has shown high estrus response and conception rate in crossbred dairy cattle using this CIDR - PGF2α - eCG based estrus synchronization protocol. Application of this protocol is highly recommended to enhance fertility of dairy cattle in the study area, and this could readily be applied to other regions.},
 year = {2016}
}

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TY  - JOUR
T1  - Manipulation of Fertility in Crossbred Dairy Cattle Using Controlled Internal Drug Release Device Combined With Prostaglandin and Equine Chorionic Gonadotrophin
AU  - Alemselam Birhanu Mekonnin
AU  - A. Forbes Howie
AU  - Christopher R. Harlow
AU  - Goitom Gidey
AU  - Desalew Tadesse Tegegne
AU  - Gidena Desta
AU  - Tadesse Gugsa
AU  - Berihu Gebrekidan
AU  - Gebregiorgis Ashebir
AU  - Simon C. Riley
Y1  - 2016/05/23
PY  - 2016
N1  - https://doi.org/10.11648/j.avs.20160403.12
DO  - 10.11648/j.avs.20160403.12
T2  - Animal and Veterinary Sciences
JF  - Animal and Veterinary Sciences
JO  - Animal and Veterinary Sciences
SP  - 39
EP  - 46
PB  - Science Publishing Group
SN  - 2328-5850
UR  - https://doi.org/10.11648/j.avs.20160403.12
AB  - Simplifying and improving our understanding of the protocols for inducing or synchronizing estrus is important for improving management of dairy cattle. This study evaluated the estrus response and conception rate of crossbred (Holstein Friesian X Zebu) dairy cows (n=75) and heifers (n=47) assigned to a 10-day controlled internal drug release (CIDR) device – prostaglandin F2-alpha (PGF2α) – equine chorionic gonadotrophin (eCG) based estrus synchronization protocol. Animals were assigned to three groups (a) anestrus (n=62), (b) repeat-breeders (n=11) and (c) silent estrus (n=46), according to their reproductive history, per rectal palpation, and progesterone concentrations determined by On-Farm enzyme-linked immunosorbent assays (ELISA) in milk (lactating cows) or serum (dry cows and heifers). For each animal, a CIDR device (containing 1.38g progesterone) was inserted into the vagina and allowed to remain in-situ for 10 days. On Day 8 after device insertion, a dose of 500µg of PGF2α was administered intramuscularly (IM). On Day 10 (device removal), 500 IU of eCG was given IM. Animals were inseminated or mated 48 and 72 hrs post device removal. Pregnancy was assessed 20-24 days post insemination by measurement of progesterone in milk/serum and pregnancy was confirmed by per rectal palpation and/or ultrasonography 44-90 days post insemination. Overall estrus response and conception rates were 97.5% and 78.3%, respectively, with no significant differences in parity, pre-treatment reproductive status and farming system (smallholder vs organized commercial farms). Conception rate after induced/synchronized estrus was 82.9% and 72.1% in cows and heifers, respectively, with an overall calving rate of 94.4%. In conclusion, the present study has shown high estrus response and conception rate in crossbred dairy cattle using this CIDR - PGF2α - eCG based estrus synchronization protocol. Application of this protocol is highly recommended to enhance fertility of dairy cattle in the study area, and this could readily be applied to other regions.
VL  - 4
IS  - 3
ER  -

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