This study aimed to obtain efficient bacteria capable of desulfurizing dibenzothiophene (DBT). For this purpose forty oil contaminated soil samples were collected from different sites in Iraq. It was found that three isolates (M9, M19 and S25) had the ability to desulfurize DBT (cleave C-S bond) and converted it to 2-hydroxybiphenel (2-HBP) or other phenolic end products. This suggests the involvement of the 4S pathway in the desulfurizing of DBT via a specific cleavage of only the C-S bond by these isolates. These isolates were identified as Pseudomonas aeruginosa. The result also showed that P. aeruginosa S25 was the most efficient one for removing sulfur from DBT. The GC/MS analysis for DBT after growth of P. aeruginosa S25, indicated that 12.89 % of DBT was consumed (consumption of sulfur), and the product (2-HBP) was further converted to 2-MBP (addition of a methyl group) and this could be a novel pathway for consuming DBT.
| Published in | American Journal of Bioscience and Bioengineering (Volume 3, Issue 5) |
| DOI | 10.11648/j.bio.20150305.13 |
| Page(s) | 40-46 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2015. Published by Science Publishing Group |
Dibenzothiophene, Biodesulfurization, Isolation, P. aeruginosa, GC/MS
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APA Style
Majid Hussein Al-Jailawi, Albab Fawaz Al-Faraas, Abdelghani Ibrahem Yahia. (2015). Isolation and Identification of Dibenzothiophene Biodesulfurizing Bacteria. American Journal of Bioscience and Bioengineering, 3(5), 40-46. https://doi.org/10.11648/j.bio.20150305.13
ACS Style
Majid Hussein Al-Jailawi; Albab Fawaz Al-Faraas; Abdelghani Ibrahem Yahia. Isolation and Identification of Dibenzothiophene Biodesulfurizing Bacteria. Am. J. BioSci. Bioeng. 2015, 3(5), 40-46. doi: 10.11648/j.bio.20150305.13
AMA Style
Majid Hussein Al-Jailawi, Albab Fawaz Al-Faraas, Abdelghani Ibrahem Yahia. Isolation and Identification of Dibenzothiophene Biodesulfurizing Bacteria. Am J BioSci Bioeng. 2015;3(5):40-46. doi: 10.11648/j.bio.20150305.13
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Download@article{10.11648/j.bio.20150305.13,
author = {Majid Hussein Al-Jailawi and Albab Fawaz Al-Faraas and Abdelghani Ibrahem Yahia},
title = {Isolation and Identification of Dibenzothiophene Biodesulfurizing Bacteria},
journal = {American Journal of Bioscience and Bioengineering},
volume = {3},
number = {5},
pages = {40-46},
doi = {10.11648/j.bio.20150305.13},
url = {https://doi.org/10.11648/j.bio.20150305.13},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bio.20150305.13},
abstract = {This study aimed to obtain efficient bacteria capable of desulfurizing dibenzothiophene (DBT). For this purpose forty oil contaminated soil samples were collected from different sites in Iraq. It was found that three isolates (M9, M19 and S25) had the ability to desulfurize DBT (cleave C-S bond) and converted it to 2-hydroxybiphenel (2-HBP) or other phenolic end products. This suggests the involvement of the 4S pathway in the desulfurizing of DBT via a specific cleavage of only the C-S bond by these isolates. These isolates were identified as Pseudomonas aeruginosa. The result also showed that P. aeruginosa S25 was the most efficient one for removing sulfur from DBT. The GC/MS analysis for DBT after growth of P. aeruginosa S25, indicated that 12.89 % of DBT was consumed (consumption of sulfur), and the product (2-HBP) was further converted to 2-MBP (addition of a methyl group) and this could be a novel pathway for consuming DBT.},
year = {2015}
}
TY - JOUR T1 - Isolation and Identification of Dibenzothiophene Biodesulfurizing Bacteria AU - Majid Hussein Al-Jailawi AU - Albab Fawaz Al-Faraas AU - Abdelghani Ibrahem Yahia Y1 - 2015/09/26 PY - 2015 N1 - https://doi.org/10.11648/j.bio.20150305.13 DO - 10.11648/j.bio.20150305.13 T2 - American Journal of Bioscience and Bioengineering JF - American Journal of Bioscience and Bioengineering JO - American Journal of Bioscience and Bioengineering SP - 40 EP - 46 PB - Science Publishing Group SN - 2328-5893 UR - https://doi.org/10.11648/j.bio.20150305.13 AB - This study aimed to obtain efficient bacteria capable of desulfurizing dibenzothiophene (DBT). For this purpose forty oil contaminated soil samples were collected from different sites in Iraq. It was found that three isolates (M9, M19 and S25) had the ability to desulfurize DBT (cleave C-S bond) and converted it to 2-hydroxybiphenel (2-HBP) or other phenolic end products. This suggests the involvement of the 4S pathway in the desulfurizing of DBT via a specific cleavage of only the C-S bond by these isolates. These isolates were identified as Pseudomonas aeruginosa. The result also showed that P. aeruginosa S25 was the most efficient one for removing sulfur from DBT. The GC/MS analysis for DBT after growth of P. aeruginosa S25, indicated that 12.89 % of DBT was consumed (consumption of sulfur), and the product (2-HBP) was further converted to 2-MBP (addition of a methyl group) and this could be a novel pathway for consuming DBT. VL - 3 IS - 5 ER -
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