Characterization of ATP-binding Cassette Transporter Genes in Silkworm, Bombyx Mori

Fengpeng Li; Xuefang Wang; Ying Xu; Jinmei Wu

doi:doi:10.11648/j.bio.20150305.27

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Characterization of ATP-binding Cassette Transporter Genes in Silkworm, Bombyx Mori

Fengpeng Li, Xuefang Wang, Ying Xu, Jinmei Wu

Published in American Journal of Bioscience and Bioengineering (Volume 3, Issue 5)

Received: 3 December 2015 Published: 3 December 2015

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Abstract

Background: ATP-binding cassette (ABC) transporters are transmembrane proteins that utilize the energy of adenosine triphosphate (ATP) binding and hydrolysis to transport various substrates across extra and intracellular membranes, including metabolic products, lipids and sterols, and drugs. They play important roles in various processes of life, especially in drug resistance, metabolism and development. Objective: Identify the ATP-binding cassette (ABC) transporter gene family and their members in the genome of silkworm, Bombyx mori. Method: Bioinformatics and phylogenetic analysis were used in the study. Results: We identified 47 ABC proteins in the silkworm genome, which possesses members of all current ABC subfamilies A to H. ABC proteins of silkworm were compared to those from worm, fruit fly and human. A high conservation of silkworm ABC transporters were observed for proteins involved in fundamental cellular processes, including the half transporters of the ABCB subfamily, which function in iron metabolism and transport of Fe/S protein precursors, and the members of subfamilies ABCD, ABCE and ABCF, which have roles in very long chain fatty acid transport. Both ABCE and F gene products may be involved in an innate immune response to viral infections. As in the fly, ABCH proteins are inverse half-transporters showing the same domain architecture as the members of the ABCG subfamily, and ABCG transporters involve in transportation of ommochrome precursors and uric acid into pigment granules and urate granules. Conclusion: These results paved the way for further study on the function of the ABC transporters in silkworm, Bombyx mori.

Published in	American Journal of Bioscience and Bioengineering (Volume 3, Issue 5)
DOI	10.11648/j.bio.20150305.27
Page(s)	123-133
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2015. Published by Science Publishing Group

Keywords

ATP-binding Cassette Transporters, Silkworm, ABCG, Cholesterol Efflux, Multidrug Resistance-associated Protein, Insect

References

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APA Style

Fengpeng Li, Xuefang Wang, Ying Xu, Jinmei Wu. (2015). Characterization of ATP-binding Cassette Transporter Genes in Silkworm, Bombyx Mori. American Journal of Bioscience and Bioengineering, 3(5), 123-133. https://doi.org/10.11648/j.bio.20150305.27

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ACS Style

Fengpeng Li; Xuefang Wang; Ying Xu; Jinmei Wu. Characterization of ATP-binding Cassette Transporter Genes in Silkworm, Bombyx Mori. Am. J. BioSci. Bioeng. 2015, 3(5), 123-133. doi: 10.11648/j.bio.20150305.27

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AMA Style

Fengpeng Li, Xuefang Wang, Ying Xu, Jinmei Wu. Characterization of ATP-binding Cassette Transporter Genes in Silkworm, Bombyx Mori. Am J BioSci Bioeng. 2015;3(5):123-133. doi: 10.11648/j.bio.20150305.27

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@article{10.11648/j.bio.20150305.27,
  author = {Fengpeng Li and Xuefang Wang and Ying Xu and Jinmei Wu},
  title = {Characterization of ATP-binding Cassette Transporter Genes in Silkworm, Bombyx Mori},
  journal = {American Journal of Bioscience and Bioengineering},
  volume = {3},
  number = {5},
  pages = {123-133},
  doi = {10.11648/j.bio.20150305.27},
  url = {https://doi.org/10.11648/j.bio.20150305.27},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bio.20150305.27},
  abstract = {Background: ATP-binding cassette (ABC) transporters are transmembrane proteins that utilize the energy of adenosine triphosphate (ATP) binding and hydrolysis to transport various substrates across extra and intracellular membranes, including metabolic products, lipids and sterols, and drugs. They play important roles in various processes of life, especially in drug resistance, metabolism and development. Objective: Identify the ATP-binding cassette (ABC) transporter gene family and their members in the genome of silkworm, Bombyx mori. Method: Bioinformatics and phylogenetic analysis were used in the study. Results: We identified 47 ABC proteins in the silkworm genome, which possesses members of all current ABC subfamilies A to H. ABC proteins of silkworm were compared to those from worm, fruit fly and human. A high conservation of silkworm ABC transporters were observed for proteins involved in fundamental cellular processes, including the half transporters of the ABCB subfamily, which function in iron metabolism and transport of Fe/S protein precursors, and the members of subfamilies ABCD, ABCE and ABCF, which have roles in very long chain fatty acid transport. Both ABCE and F gene products may be involved in an innate immune response to viral infections. As in the fly, ABCH proteins are inverse half-transporters showing the same domain architecture as the members of the ABCG subfamily, and ABCG transporters involve in transportation of ommochrome precursors and uric acid into pigment granules and urate granules. Conclusion: These results paved the way for further study on the function of the ABC transporters in silkworm, Bombyx mori.},
 year = {2015}
}

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TY  - JOUR
T1  - Characterization of ATP-binding Cassette Transporter Genes in Silkworm, Bombyx Mori
AU  - Fengpeng Li
AU  - Xuefang Wang
AU  - Ying Xu
AU  - Jinmei Wu
Y1  - 2015/12/03
PY  - 2015
N1  - https://doi.org/10.11648/j.bio.20150305.27
DO  - 10.11648/j.bio.20150305.27
T2  - American Journal of Bioscience and Bioengineering
JF  - American Journal of Bioscience and Bioengineering
JO  - American Journal of Bioscience and Bioengineering
SP  - 123
EP  - 133
PB  - Science Publishing Group
SN  - 2328-5893
UR  - https://doi.org/10.11648/j.bio.20150305.27
AB  - Background: ATP-binding cassette (ABC) transporters are transmembrane proteins that utilize the energy of adenosine triphosphate (ATP) binding and hydrolysis to transport various substrates across extra and intracellular membranes, including metabolic products, lipids and sterols, and drugs. They play important roles in various processes of life, especially in drug resistance, metabolism and development. Objective: Identify the ATP-binding cassette (ABC) transporter gene family and their members in the genome of silkworm, Bombyx mori. Method: Bioinformatics and phylogenetic analysis were used in the study. Results: We identified 47 ABC proteins in the silkworm genome, which possesses members of all current ABC subfamilies A to H. ABC proteins of silkworm were compared to those from worm, fruit fly and human. A high conservation of silkworm ABC transporters were observed for proteins involved in fundamental cellular processes, including the half transporters of the ABCB subfamily, which function in iron metabolism and transport of Fe/S protein precursors, and the members of subfamilies ABCD, ABCE and ABCF, which have roles in very long chain fatty acid transport. Both ABCE and F gene products may be involved in an innate immune response to viral infections. As in the fly, ABCH proteins are inverse half-transporters showing the same domain architecture as the members of the ABCG subfamily, and ABCG transporters involve in transportation of ommochrome precursors and uric acid into pigment granules and urate granules. Conclusion: These results paved the way for further study on the function of the ABC transporters in silkworm, Bombyx mori.
VL  - 3
IS  - 5
ER  -

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Author Information

Fengpeng Li

College of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang City, P. R. China
Xuefang Wang

College of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang City, P. R. China
Ying Xu

College of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang City, P. R. China
Jinmei Wu

College of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang City, P. R. China

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APA Style

Fengpeng Li, Xuefang Wang, Ying Xu, Jinmei Wu. (2015). Characterization of ATP-binding Cassette Transporter Genes in Silkworm, Bombyx Mori. American Journal of Bioscience and Bioengineering, 3(5), 123-133. https://doi.org/10.11648/j.bio.20150305.27

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ACS Style

Fengpeng Li; Xuefang Wang; Ying Xu; Jinmei Wu. Characterization of ATP-binding Cassette Transporter Genes in Silkworm, Bombyx Mori. Am. J. BioSci. Bioeng. 2015, 3(5), 123-133. doi: 10.11648/j.bio.20150305.27

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AMA Style

Fengpeng Li, Xuefang Wang, Ying Xu, Jinmei Wu. Characterization of ATP-binding Cassette Transporter Genes in Silkworm, Bombyx Mori. Am J BioSci Bioeng. 2015;3(5):123-133. doi: 10.11648/j.bio.20150305.27

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@article{10.11648/j.bio.20150305.27,
  author = {Fengpeng Li and Xuefang Wang and Ying Xu and Jinmei Wu},
  title = {Characterization of ATP-binding Cassette Transporter Genes in Silkworm, Bombyx Mori},
  journal = {American Journal of Bioscience and Bioengineering},
  volume = {3},
  number = {5},
  pages = {123-133},
  doi = {10.11648/j.bio.20150305.27},
  url = {https://doi.org/10.11648/j.bio.20150305.27},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bio.20150305.27},
  abstract = {Background: ATP-binding cassette (ABC) transporters are transmembrane proteins that utilize the energy of adenosine triphosphate (ATP) binding and hydrolysis to transport various substrates across extra and intracellular membranes, including metabolic products, lipids and sterols, and drugs. They play important roles in various processes of life, especially in drug resistance, metabolism and development. Objective: Identify the ATP-binding cassette (ABC) transporter gene family and their members in the genome of silkworm, Bombyx mori. Method: Bioinformatics and phylogenetic analysis were used in the study. Results: We identified 47 ABC proteins in the silkworm genome, which possesses members of all current ABC subfamilies A to H. ABC proteins of silkworm were compared to those from worm, fruit fly and human. A high conservation of silkworm ABC transporters were observed for proteins involved in fundamental cellular processes, including the half transporters of the ABCB subfamily, which function in iron metabolism and transport of Fe/S protein precursors, and the members of subfamilies ABCD, ABCE and ABCF, which have roles in very long chain fatty acid transport. Both ABCE and F gene products may be involved in an innate immune response to viral infections. As in the fly, ABCH proteins are inverse half-transporters showing the same domain architecture as the members of the ABCG subfamily, and ABCG transporters involve in transportation of ommochrome precursors and uric acid into pigment granules and urate granules. Conclusion: These results paved the way for further study on the function of the ABC transporters in silkworm, Bombyx mori.},
 year = {2015}
}

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TY  - JOUR
T1  - Characterization of ATP-binding Cassette Transporter Genes in Silkworm, Bombyx Mori
AU  - Fengpeng Li
AU  - Xuefang Wang
AU  - Ying Xu
AU  - Jinmei Wu
Y1  - 2015/12/03
PY  - 2015
N1  - https://doi.org/10.11648/j.bio.20150305.27
DO  - 10.11648/j.bio.20150305.27
T2  - American Journal of Bioscience and Bioengineering
JF  - American Journal of Bioscience and Bioengineering
JO  - American Journal of Bioscience and Bioengineering
SP  - 123
EP  - 133
PB  - Science Publishing Group
SN  - 2328-5893
UR  - https://doi.org/10.11648/j.bio.20150305.27
AB  - Background: ATP-binding cassette (ABC) transporters are transmembrane proteins that utilize the energy of adenosine triphosphate (ATP) binding and hydrolysis to transport various substrates across extra and intracellular membranes, including metabolic products, lipids and sterols, and drugs. They play important roles in various processes of life, especially in drug resistance, metabolism and development. Objective: Identify the ATP-binding cassette (ABC) transporter gene family and their members in the genome of silkworm, Bombyx mori. Method: Bioinformatics and phylogenetic analysis were used in the study. Results: We identified 47 ABC proteins in the silkworm genome, which possesses members of all current ABC subfamilies A to H. ABC proteins of silkworm were compared to those from worm, fruit fly and human. A high conservation of silkworm ABC transporters were observed for proteins involved in fundamental cellular processes, including the half transporters of the ABCB subfamily, which function in iron metabolism and transport of Fe/S protein precursors, and the members of subfamilies ABCD, ABCE and ABCF, which have roles in very long chain fatty acid transport. Both ABCE and F gene products may be involved in an innate immune response to viral infections. As in the fly, ABCH proteins are inverse half-transporters showing the same domain architecture as the members of the ABCG subfamily, and ABCG transporters involve in transportation of ommochrome precursors and uric acid into pigment granules and urate granules. Conclusion: These results paved the way for further study on the function of the ABC transporters in silkworm, Bombyx mori.
VL  - 3
IS  - 5
ER  -

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