Histopathological Study and Oxidative Stress, Antioxidants Parameters and Liver Enzymes Activity Determination of <i>Cyperus</i> Methanolic Extract and Glucophage Drug on Albino Male Mice

Ruqaya Mohammed Al-Ezzy; Sura Alaa Soud; Zahraa Mohand Sadoon

doi:doi:10.11648/j.bio.20190701.12

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Histopathological Study and Oxidative Stress, Antioxidants Parameters and Liver Enzymes Activity Determination of Cyperus Methanolic Extract and Glucophage Drug on Albino Male Mice

Ruqaya Mohammed Al-Ezzy, Sura Alaa Soud, Zahraa Mohand Sadoon

Published in American Journal of Bioscience and Bioengineering (Volume 7, Issue 1)

Received: 26 February 2019 Accepted: 2 April 2019 Published: 26 April 2019

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Abstract

The study aimed to explain the hepatoprotective action of Cyperus rotundus methanolic extract in vitro and in vivo (albino male mice). An interaction between the extract and Glucophage drug were also made to evaluate the in vivo modulating effects of the extract. The hepatoprotective evaluations included assessment of malondialdyde (MDA) and catalase (CAT) activities and liver function enzymes (aspartate aminotransferase; AST and alanine aminotransferase; ALT and alkaline phosphatase; ALP) in blood serum, as well as histopathological estimation of liver tissue, in mice after administration of the drug Glucophage and cyperus rotundus methanolic extract alone or with interaction. The results indicated that the plant extract (200mg/kg) decreased MDA activity and increase significantly activity of catalase in comparison to positive and negative controls. Also Cyperus rotundus methanolic increase the level of ALT, AST and ALP. The drug Glucophage was contributed to increased MDA activity and decreased the activity of catalase in comparison to positive and negative controls in addition to increase the activity of ALT, AST and ALP. The interaction between the extract and glucophage increased both MDA and catalase activity in comparison to control group. In addition to increase the liver function enzymes ALT, AST and ALP. The histopathological activity represented that histopathological section in the liver of mouse treated with Cyperus rotundus extraction showed scattered degenerated cells while histopathological section in the liver of mouse treated with Glucophage showed normal architecture with few pyknotic nuclei. Histopathological section in the liver of mouse treated with Cyperus rotundus and Glucophage showed multiple aggregation mononuclear cells around central vein.

Published in	American Journal of Bioscience and Bioengineering (Volume 7, Issue 1)
DOI	10.11648/j.bio.20190701.12
Page(s)	10-15
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2019. Published by Science Publishing Group

Keywords

Histopathological Study and Oxidative Stress, Antioxidants Parameters and Liver Enzymes Activity Determination of Cyperus Methanolic Extract and Glucophage Drug on Albino Male Mice The study aimed to explain the hepatoprotective action of Cyperus rotundus methanolic extract in vitro and in vivo (albino male mice). An interaction between the extract and Glucophage drug were also made to evaluate the in vivo modulating effects of the extract. The hepatoprotective evalua

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APA Style

Ruqaya Mohammed Al-Ezzy, Sura Alaa Soud, Zahraa Mohand Sadoon. (2019). Histopathological Study and Oxidative Stress, Antioxidants Parameters and Liver Enzymes Activity Determination of Cyperus Methanolic Extract and Glucophage Drug on Albino Male Mice. American Journal of Bioscience and Bioengineering, 7(1), 10-15. https://doi.org/10.11648/j.bio.20190701.12

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ACS Style

Ruqaya Mohammed Al-Ezzy; Sura Alaa Soud; Zahraa Mohand Sadoon. Histopathological Study and Oxidative Stress, Antioxidants Parameters and Liver Enzymes Activity Determination of Cyperus Methanolic Extract and Glucophage Drug on Albino Male Mice. Am. J. BioSci. Bioeng. 2019, 7(1), 10-15. doi: 10.11648/j.bio.20190701.12

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AMA Style

Ruqaya Mohammed Al-Ezzy, Sura Alaa Soud, Zahraa Mohand Sadoon. Histopathological Study and Oxidative Stress, Antioxidants Parameters and Liver Enzymes Activity Determination of Cyperus Methanolic Extract and Glucophage Drug on Albino Male Mice. Am J BioSci Bioeng. 2019;7(1):10-15. doi: 10.11648/j.bio.20190701.12

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@article{10.11648/j.bio.20190701.12,
  author = {Ruqaya Mohammed Al-Ezzy and Sura Alaa Soud and Zahraa Mohand Sadoon},
  title = {Histopathological Study and Oxidative Stress, Antioxidants Parameters and Liver Enzymes Activity Determination of Cyperus Methanolic Extract and Glucophage Drug on Albino Male Mice},
  journal = {American Journal of Bioscience and Bioengineering},
  volume = {7},
  number = {1},
  pages = {10-15},
  doi = {10.11648/j.bio.20190701.12},
  url = {https://doi.org/10.11648/j.bio.20190701.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bio.20190701.12},
  abstract = {The study aimed to explain the hepatoprotective action of Cyperus rotundus methanolic extract in vitro and in vivo (albino male mice). An interaction between the extract and Glucophage drug were also made to evaluate the in vivo modulating effects of the extract. The hepatoprotective evaluations included assessment of malondialdyde (MDA) and catalase (CAT) activities and liver function enzymes (aspartate aminotransferase; AST and alanine aminotransferase; ALT and alkaline phosphatase; ALP) in blood serum, as well as histopathological estimation of liver tissue, in mice after administration of the drug Glucophage and cyperus rotundus methanolic extract alone or with interaction. The results indicated that the plant extract (200mg/kg) decreased MDA activity and increase significantly activity of catalase in comparison to positive and negative controls. Also Cyperus rotundus methanolic increase the level of ALT, AST and ALP. The drug Glucophage was contributed to increased MDA activity and decreased the activity of catalase in comparison to positive and negative controls in addition to increase the activity of ALT, AST and ALP. The interaction between the extract and glucophage increased both MDA and catalase activity in comparison to control group. In addition to increase the liver function enzymes ALT, AST and ALP. The histopathological activity represented that histopathological section in the liver of mouse treated with Cyperus rotundus extraction showed scattered degenerated cells while histopathological section in the liver of mouse treated with Glucophage showed normal architecture with few pyknotic nuclei. Histopathological section in the liver of mouse treated with Cyperus rotundus and Glucophage showed multiple aggregation mononuclear cells around central vein.},
 year = {2019}
}

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TY  - JOUR
T1  - Histopathological Study and Oxidative Stress, Antioxidants Parameters and Liver Enzymes Activity Determination of Cyperus Methanolic Extract and Glucophage Drug on Albino Male Mice
AU  - Ruqaya Mohammed Al-Ezzy
AU  - Sura Alaa Soud
AU  - Zahraa Mohand Sadoon
Y1  - 2019/04/26
PY  - 2019
N1  - https://doi.org/10.11648/j.bio.20190701.12
DO  - 10.11648/j.bio.20190701.12
T2  - American Journal of Bioscience and Bioengineering
JF  - American Journal of Bioscience and Bioengineering
JO  - American Journal of Bioscience and Bioengineering
SP  - 10
EP  - 15
PB  - Science Publishing Group
SN  - 2328-5893
UR  - https://doi.org/10.11648/j.bio.20190701.12
AB  - The study aimed to explain the hepatoprotective action of Cyperus rotundus methanolic extract in vitro and in vivo (albino male mice). An interaction between the extract and Glucophage drug were also made to evaluate the in vivo modulating effects of the extract. The hepatoprotective evaluations included assessment of malondialdyde (MDA) and catalase (CAT) activities and liver function enzymes (aspartate aminotransferase; AST and alanine aminotransferase; ALT and alkaline phosphatase; ALP) in blood serum, as well as histopathological estimation of liver tissue, in mice after administration of the drug Glucophage and cyperus rotundus methanolic extract alone or with interaction. The results indicated that the plant extract (200mg/kg) decreased MDA activity and increase significantly activity of catalase in comparison to positive and negative controls. Also Cyperus rotundus methanolic increase the level of ALT, AST and ALP. The drug Glucophage was contributed to increased MDA activity and decreased the activity of catalase in comparison to positive and negative controls in addition to increase the activity of ALT, AST and ALP. The interaction between the extract and glucophage increased both MDA and catalase activity in comparison to control group. In addition to increase the liver function enzymes ALT, AST and ALP. The histopathological activity represented that histopathological section in the liver of mouse treated with Cyperus rotundus extraction showed scattered degenerated cells while histopathological section in the liver of mouse treated with Glucophage showed normal architecture with few pyknotic nuclei. Histopathological section in the liver of mouse treated with Cyperus rotundus and Glucophage showed multiple aggregation mononuclear cells around central vein.
VL  - 7
IS  - 1
ER  -

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Author Information

Ruqaya Mohammed Al-Ezzy

Departement of Molecular and Medical Biotechnology, College of Biotechnology, Al-Nahrain University, Baghdad, Iraq
Sura Alaa Soud

Departement of Microbiology, College of Science, Al-Karkh University of Science, Baghdad, Iraq
Zahraa Mohand Sadoon

Departement of Molecular and Medical Biotechnology, College of Biotechnology, Al-Nahrain University, Baghdad, Iraq

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Ruqaya Mohammed Al-Ezzy, Sura Alaa Soud, Zahraa Mohand Sadoon. (2019). Histopathological Study and Oxidative Stress, Antioxidants Parameters and Liver Enzymes Activity Determination of Cyperus Methanolic Extract and Glucophage Drug on Albino Male Mice. American Journal of Bioscience and Bioengineering, 7(1), 10-15. https://doi.org/10.11648/j.bio.20190701.12

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ACS Style

Ruqaya Mohammed Al-Ezzy; Sura Alaa Soud; Zahraa Mohand Sadoon. Histopathological Study and Oxidative Stress, Antioxidants Parameters and Liver Enzymes Activity Determination of Cyperus Methanolic Extract and Glucophage Drug on Albino Male Mice. Am. J. BioSci. Bioeng. 2019, 7(1), 10-15. doi: 10.11648/j.bio.20190701.12

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AMA Style

Ruqaya Mohammed Al-Ezzy, Sura Alaa Soud, Zahraa Mohand Sadoon. Histopathological Study and Oxidative Stress, Antioxidants Parameters and Liver Enzymes Activity Determination of Cyperus Methanolic Extract and Glucophage Drug on Albino Male Mice. Am J BioSci Bioeng. 2019;7(1):10-15. doi: 10.11648/j.bio.20190701.12

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@article{10.11648/j.bio.20190701.12,
  author = {Ruqaya Mohammed Al-Ezzy and Sura Alaa Soud and Zahraa Mohand Sadoon},
  title = {Histopathological Study and Oxidative Stress, Antioxidants Parameters and Liver Enzymes Activity Determination of Cyperus Methanolic Extract and Glucophage Drug on Albino Male Mice},
  journal = {American Journal of Bioscience and Bioengineering},
  volume = {7},
  number = {1},
  pages = {10-15},
  doi = {10.11648/j.bio.20190701.12},
  url = {https://doi.org/10.11648/j.bio.20190701.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bio.20190701.12},
  abstract = {The study aimed to explain the hepatoprotective action of Cyperus rotundus methanolic extract in vitro and in vivo (albino male mice). An interaction between the extract and Glucophage drug were also made to evaluate the in vivo modulating effects of the extract. The hepatoprotective evaluations included assessment of malondialdyde (MDA) and catalase (CAT) activities and liver function enzymes (aspartate aminotransferase; AST and alanine aminotransferase; ALT and alkaline phosphatase; ALP) in blood serum, as well as histopathological estimation of liver tissue, in mice after administration of the drug Glucophage and cyperus rotundus methanolic extract alone or with interaction. The results indicated that the plant extract (200mg/kg) decreased MDA activity and increase significantly activity of catalase in comparison to positive and negative controls. Also Cyperus rotundus methanolic increase the level of ALT, AST and ALP. The drug Glucophage was contributed to increased MDA activity and decreased the activity of catalase in comparison to positive and negative controls in addition to increase the activity of ALT, AST and ALP. The interaction between the extract and glucophage increased both MDA and catalase activity in comparison to control group. In addition to increase the liver function enzymes ALT, AST and ALP. The histopathological activity represented that histopathological section in the liver of mouse treated with Cyperus rotundus extraction showed scattered degenerated cells while histopathological section in the liver of mouse treated with Glucophage showed normal architecture with few pyknotic nuclei. Histopathological section in the liver of mouse treated with Cyperus rotundus and Glucophage showed multiple aggregation mononuclear cells around central vein.},
 year = {2019}
}

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TY  - JOUR
T1  - Histopathological Study and Oxidative Stress, Antioxidants Parameters and Liver Enzymes Activity Determination of Cyperus Methanolic Extract and Glucophage Drug on Albino Male Mice
AU  - Ruqaya Mohammed Al-Ezzy
AU  - Sura Alaa Soud
AU  - Zahraa Mohand Sadoon
Y1  - 2019/04/26
PY  - 2019
N1  - https://doi.org/10.11648/j.bio.20190701.12
DO  - 10.11648/j.bio.20190701.12
T2  - American Journal of Bioscience and Bioengineering
JF  - American Journal of Bioscience and Bioengineering
JO  - American Journal of Bioscience and Bioengineering
SP  - 10
EP  - 15
PB  - Science Publishing Group
SN  - 2328-5893
UR  - https://doi.org/10.11648/j.bio.20190701.12
AB  - The study aimed to explain the hepatoprotective action of Cyperus rotundus methanolic extract in vitro and in vivo (albino male mice). An interaction between the extract and Glucophage drug were also made to evaluate the in vivo modulating effects of the extract. The hepatoprotective evaluations included assessment of malondialdyde (MDA) and catalase (CAT) activities and liver function enzymes (aspartate aminotransferase; AST and alanine aminotransferase; ALT and alkaline phosphatase; ALP) in blood serum, as well as histopathological estimation of liver tissue, in mice after administration of the drug Glucophage and cyperus rotundus methanolic extract alone or with interaction. The results indicated that the plant extract (200mg/kg) decreased MDA activity and increase significantly activity of catalase in comparison to positive and negative controls. Also Cyperus rotundus methanolic increase the level of ALT, AST and ALP. The drug Glucophage was contributed to increased MDA activity and decreased the activity of catalase in comparison to positive and negative controls in addition to increase the activity of ALT, AST and ALP. The interaction between the extract and glucophage increased both MDA and catalase activity in comparison to control group. In addition to increase the liver function enzymes ALT, AST and ALP. The histopathological activity represented that histopathological section in the liver of mouse treated with Cyperus rotundus extraction showed scattered degenerated cells while histopathological section in the liver of mouse treated with Glucophage showed normal architecture with few pyknotic nuclei. Histopathological section in the liver of mouse treated with Cyperus rotundus and Glucophage showed multiple aggregation mononuclear cells around central vein.
VL  - 7
IS  - 1
ER  -

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