Enhance Maize <i>(Zea mays L.)</i> Tolerance to Abiotic Stress Through the Genetic Transformation with Anthranilate Synthase (ASA2 Gene) Using Particle Bombardment

Karim Mohamed Beltagy; Manal Moustafa Abdel-Rahman; Hanaa Mahdy Abouzied; Samia Ahmed Madkour

doi:doi:10.11648/j.bio.20190702.11

| Peer-Reviewed

Enhance Maize (Zea mays L.) Tolerance to Abiotic Stress Through the Genetic Transformation with Anthranilate Synthase (ASA2 Gene) Using Particle Bombardment

Karim Mohamed Beltagy, Manal Moustafa Abdel-Rahman, Hanaa Mahdy Abouzied, Samia Ahmed Madkour

Published in American Journal of Bioscience and Bioengineering (Volume 7, Issue 2)

Received: 4 March 2019 Accepted: 19 March 2019 Published: 7 May 2019

Views: Downloads:

Download PDF

Share This Article

Twitter
Linked In
Facebook

Abstract

The agricultural plans in Egypt aim to increase maize production and yield to reduce the importation and to meet the country's need from maize. Currently the crop production is affected by the climatic changes which rise the abiotic and biotic stresses problems. In an attempt to raise the tryptophan level in maize, particle bombardment technique was used with two hybrids maize, namely Sc168 and Sc10. The results of this study showed that both hybrids were transformed efficiently and showed increased levels of tryptophan in the two hybrids. The double shot 1100 psi was higher effective than single shot. It can be concluded that levels of tryptophan increased clearly in the two hybrids which is considered an indicator for ASA2 successful expressing as compared to control. The present study established effective tissue culture protocol for maize hybrids suitable for gene transformation which may used in maize improvement programs in the future.

Published in	American Journal of Bioscience and Bioengineering (Volume 7, Issue 2)
DOI	10.11648/j.bio.20190702.11
Page(s)	28-33
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2019. Published by Science Publishing Group

Keywords

Maize, Biotic Stress, Anthranilate Synthase, Gus Assay, Particle Bombardment

References

[1]	Faostat Agricultural database, Food and Agriculture Organization of the United Natios (FAO) http://www.fao.org/2009.
[2]	Morgan, D. 2010. Egypt: growth and opportunity inagriculture. Available at: http://www.english.globalarabnetwork.com/201003155185/Economics/growth-and-opportunity-in-egyptian-agriculture.html
[3]	Snežana Mladenović Drinić, Dragana Ignjatović Micić, Iva Erić, Violeta anđ Elković, Dražen Jelovac, аnd Kosana Konstantinov. 2004. Biotechnology in maize breeding. Genetika, vol. 36, no. 2, 93-109, 2004.
[4]	Abdel-Rahman M. M. and Widholm J. M. 2009. Transformation of two plasmids into maize callus using particle bombardment. Journal Enviromental Agriculture Science, Faculty of Agriculture Damanhour branch, Alexandria University. Vol. 8 (2) August.
[5]	Shou H, Frame B. R., Witham S. A., and Wang K. 2004. Assessment of transgenic maize events produced by particle bombardment or Agrobacterium- mediated transformation Mol. Breeding 13: 201-208.
[6]	USDA ERS 1999. Genetically engineered crops for pest management. U.S. Dept. of agriculture, economic research service, Washington, DC. http://www.ers.usda.gov/whatnew/issues/biotech
[7]	Gordon-Kamm W. J., T. M. Spencer, M. L. Mangano, R. J. Adam S, N. G. Willets, T. B. Rice, C. J. Mackery, R. W. Krueger, A. P. Kaush, and P. G. Lemaux. 1990. Transformation of maize cells and regeneration of fertile transgenic plants. Plant Cell, 2, 603-618.
[8]	Tsuji J., Zook M., Somerville S. C., Hammerschmidt L. 1993. Evidence that tryptophan is not a direct biosynthetic intermediate of camamexin in Arabidopsis thaliana Physiol.mol. Plant Pathol. 43: 221-229.
[9]	Normanly J., Slovin J. P., Cohen J. D. 1995. Rethinking auxin biosynthesis and metabolism. Plant Physiol. 107: 323-329.
[10]	Radwanski E. R., and LAST r.l. 1995. Tryptophan biosynthesis and metabolism: biochemicaland molecular genetics. Plant Cell 7: 921-934.
[11]	Rai. V. K. 2002. Role of amino acid in plant responses to stresses. Biol. Plantarum J., 45: 481-487.
[12]	Zhao J, Last RL. 1996. Coordinate regulation of the tryptophan biosynthetic pathway and indolic phytoalexin accumulation in Arabidopsis. Plant Cel. 8: 2235-44; PMID: 8989880; http://dx.doi.org/10.1105/tpc.8.12.2235 [PMC free article, PubMed, Cross Ref].
[13]	Kanno T, Kasai K, Ikejiri-Kanno Y, Wakasa K, Tozawa Y. 2004. In vitro reconstitution of rice anthranilate synthase: MAPK signaling pathways in plant abiotic stress responses. Mol Biol. 54: 11-22; http://dx.doi.org/10.1023/B:PLAN.0000028729.79034.07 [PubMed, Cross Ref].
[14]	Chu CC, Wang CC, Sun CS, Hsu C, Yin KC, Chu CY and Bi F. 1975. Establishment of efficient medium for another culture of rice through comparative experiments on the nitrogen source. Sci. Sinica. 18: 659-668.
[15]	Murashige T and Skoog F. 1962. Revised medium for rapid growth and bioassays with tobacco tissue cultures. Plant Physiol. 15: 473-479.
[16]	Berardino MB., Roingeard F. C., Fukagawa N. K. 1990. Plasma tryptophan and tyrosine concentrations determination using high performance liquid chromatography and fluorometric detection J. Nutr. Biocheml. 220-222.
[17]	Brotherton J. E., Schechter S., Ranch J. P., Widholm J. M. 1996. Inheritance and stability of 5 methyl tryptophan resistance in Dature innoxia selected in vitro Plant Cell Physiol. 37: 389-394.
[18]	Valdez-ortiz A., Medina-godoy S., Evcerde M., Paredes-lo'pez O. 2007. Transgenic tropical maize line generated by the direct transformation of the embryo-scutellum by A. tumefaciens Plant cell tissue organ culture 9: 201-214.
[19]	Green, C. E. and R. L. Phillips. 1975. Plant regeneration from tissue cultures of maize. Crop Sci., 15: 417-420.
[20]	Straus, J. 1960. Maize endosperm tissue grown in vitro. III. Development of a synthetic medium. Am. J. Bot., 47: 641-647.
[21]	Schenk, R. U. and A. C. Hildebrandt. 1972. Medium and techniques for induction and growth of monocotyledonous and dicotyledonous plant cell cultures. Can. J. Bot., 50: 199-204.
[22]	Armstrong CL, Green CE. 1985. Establishment and maintenance of friable, embryogenic maize callus and the involvement of L-proline. Planta. 164: 207-214.
[23]	Ray DS, Ghosh PD. 1990. Somatic embryogenesis and plant regeneration from cultured leaf explants of Zea mays L. Ann. Bot. 66: 497-500.
[24]	Twyman R, Christou P, Stoger E (2002) Genetic transformation of plants and their cells, Molecular Biotechnology, John Innes Center, Norwich, United Kingdom pp. 126-150.
[25]	Frame B, Zhang H, Cocciolone S, S idorenko L, Dietrich C, Pegg S, Zhen S, SchnableP, Wang K. 2000. Production of transgenic maize from bombarded type II callus: Effect of gold particle size and callus morphologyon transformation efficiency In Vitro Cell Dev. Biol. Plant 36: 21-29.
[26]	Aulinger I, Peter S, Schmid J, Stamp P. 2003. Gametic embryos of maize as atarget for biolistic transformation: Comparison to immature zygotic embryos Plant Cell Rep. 21: 585-591.
[27]	De'cima Oneto C, Bossio E, Gonza'lez G, Faccio P, Lewi D. 2010. High and low pressure gene gun devices gie similar transformation efficiencies in maize calluses Afr. J. Plant Sci 4 (7): 217- 225.
[28]	Oneto C. D., Gonzalez G., Lewi D. 2010. Biolistic maize transformation: Improving and simplifying the protocol efficiency Afr. J. of Agri Res. 25: 3561-3570.
[29]	Gallagher SR. 1992. GUS protocols: using the GUS gene as a reporter of gene expression. Academic Press, New York.
[30]	Jefferson RA. 1987 Assaying chimeric genes in plants: the GUS gene fusion system. Plant Mol. BioI. Rep. 5: 387-405.
[31]	Jefferson RA, Kavanagh TA and Bevan MW. 1987. GUS fusion: f3-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO J. 6: 3901-3907.
[32]	Wakasa K., Tozawa Y., Terakawa T., Hasegawa H. 1999. Gene encoding α –subunit of rice anthranilate synthase and DNA relating thereto. World Intellectual Property Organization 99/11800.
[33]	Shukla V. K., Doyon, Y., Miller, J. C., Dekelver, R. C., Moehle, E. A., Worden, S. E., et al. 2009. Precise genome modification in the crop species (Zea mays L.) using zinc- finger nucleases. Nature 459, 437-441.
[34]	Gao H., Smith J., Yang M., Jones S., Djukanovic V., Nicholson M. G., et al. 2010. Heritable targeted mutagenesis in maize using a designed endonuclease. Plant J. 61: 176-187.
[35]	Liang Z., Zhang K., Chen K., and Gao C. 2014. Targeted mutagenesis in maize (Zea mays L.) using TALENs and the CRISPR/Cas system J. Genet. Genomics 41: 63-68.

Cite This Article

Plain Text BibTeX RIS

APA Style

Karim Mohamed Beltagy, Manal Moustafa Abdel-Rahman, Hanaa Mahdy Abouzied, Samia Ahmed Madkour. (2019). Enhance Maize (Zea mays L.) Tolerance to Abiotic Stress Through the Genetic Transformation with Anthranilate Synthase (ASA2 Gene) Using Particle Bombardment. American Journal of Bioscience and Bioengineering, 7(2), 28-33. https://doi.org/10.11648/j.bio.20190702.11

Copy | Download

ACS Style

Karim Mohamed Beltagy; Manal Moustafa Abdel-Rahman; Hanaa Mahdy Abouzied; Samia Ahmed Madkour. Enhance Maize (Zea mays L.) Tolerance to Abiotic Stress Through the Genetic Transformation with Anthranilate Synthase (ASA2 Gene) Using Particle Bombardment. Am. J. BioSci. Bioeng. 2019, 7(2), 28-33. doi: 10.11648/j.bio.20190702.11

Copy | Download

AMA Style

Karim Mohamed Beltagy, Manal Moustafa Abdel-Rahman, Hanaa Mahdy Abouzied, Samia Ahmed Madkour. Enhance Maize (Zea mays L.) Tolerance to Abiotic Stress Through the Genetic Transformation with Anthranilate Synthase (ASA2 Gene) Using Particle Bombardment. Am J BioSci Bioeng. 2019;7(2):28-33. doi: 10.11648/j.bio.20190702.11

Copy | Download

@article{10.11648/j.bio.20190702.11,
  author = {Karim Mohamed Beltagy and Manal Moustafa Abdel-Rahman and Hanaa Mahdy Abouzied and Samia Ahmed Madkour},
  title = {Enhance Maize (Zea mays L.) Tolerance to Abiotic Stress Through the Genetic Transformation with Anthranilate Synthase (ASA2 Gene) Using Particle Bombardment},
  journal = {American Journal of Bioscience and Bioengineering},
  volume = {7},
  number = {2},
  pages = {28-33},
  doi = {10.11648/j.bio.20190702.11},
  url = {https://doi.org/10.11648/j.bio.20190702.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bio.20190702.11},
  abstract = {The agricultural plans in Egypt aim to increase maize production and yield to reduce the importation and to meet the country's need from maize. Currently the crop production is affected by the climatic changes which rise the abiotic and biotic stresses problems. In an attempt to raise the tryptophan level in maize, particle bombardment technique was used with two hybrids maize, namely Sc168 and Sc10. The results of this study showed that both hybrids were transformed efficiently and showed increased levels of tryptophan in the two hybrids. The double shot 1100 psi was higher effective than single shot. It can be concluded that levels of tryptophan increased clearly in the two hybrids which is considered an indicator for ASA2 successful expressing as compared to control. The present study established effective tissue culture protocol for maize hybrids suitable for gene transformation which may used in maize improvement programs in the future.},
 year = {2019}
}

Copy | Download

TY  - JOUR
T1  - Enhance Maize (Zea mays L.) Tolerance to Abiotic Stress Through the Genetic Transformation with Anthranilate Synthase (ASA2 Gene) Using Particle Bombardment
AU  - Karim Mohamed Beltagy
AU  - Manal Moustafa Abdel-Rahman
AU  - Hanaa Mahdy Abouzied
AU  - Samia Ahmed Madkour
Y1  - 2019/05/07
PY  - 2019
N1  - https://doi.org/10.11648/j.bio.20190702.11
DO  - 10.11648/j.bio.20190702.11
T2  - American Journal of Bioscience and Bioengineering
JF  - American Journal of Bioscience and Bioengineering
JO  - American Journal of Bioscience and Bioengineering
SP  - 28
EP  - 33
PB  - Science Publishing Group
SN  - 2328-5893
UR  - https://doi.org/10.11648/j.bio.20190702.11
AB  - The agricultural plans in Egypt aim to increase maize production and yield to reduce the importation and to meet the country's need from maize. Currently the crop production is affected by the climatic changes which rise the abiotic and biotic stresses problems. In an attempt to raise the tryptophan level in maize, particle bombardment technique was used with two hybrids maize, namely Sc168 and Sc10. The results of this study showed that both hybrids were transformed efficiently and showed increased levels of tryptophan in the two hybrids. The double shot 1100 psi was higher effective than single shot. It can be concluded that levels of tryptophan increased clearly in the two hybrids which is considered an indicator for ASA2 successful expressing as compared to control. The present study established effective tissue culture protocol for maize hybrids suitable for gene transformation which may used in maize improvement programs in the future.
VL  - 7
IS  - 2
ER  -

Copy | Download

Author Information

Karim Mohamed Beltagy

Plant Pathology Department (Genetic Branch), Faculty of Agriculture, Damanhour University, Damanhour, Egypt
Manal Moustafa Abdel-Rahman

Plant Pathology Department (Genetic Branch), Faculty of Agriculture, Damanhour University, Damanhour, Egypt
Hanaa Mahdy Abouzied

Agronomy Department, Faculty of Agriculture, Damanhour University, Damanhour, Egypt
Samia Ahmed Madkour

Plant Pathology Department (Genetic Branch), Faculty of Agriculture, Damanhour University, Damanhour, Egypt

Download PDF

Sections

Plain Text BibTeX RIS

APA Style

Karim Mohamed Beltagy, Manal Moustafa Abdel-Rahman, Hanaa Mahdy Abouzied, Samia Ahmed Madkour. (2019). Enhance Maize (Zea mays L.) Tolerance to Abiotic Stress Through the Genetic Transformation with Anthranilate Synthase (ASA2 Gene) Using Particle Bombardment. American Journal of Bioscience and Bioengineering, 7(2), 28-33. https://doi.org/10.11648/j.bio.20190702.11

Copy | Download

ACS Style

Karim Mohamed Beltagy; Manal Moustafa Abdel-Rahman; Hanaa Mahdy Abouzied; Samia Ahmed Madkour. Enhance Maize (Zea mays L.) Tolerance to Abiotic Stress Through the Genetic Transformation with Anthranilate Synthase (ASA2 Gene) Using Particle Bombardment. Am. J. BioSci. Bioeng. 2019, 7(2), 28-33. doi: 10.11648/j.bio.20190702.11

Copy | Download

AMA Style

Karim Mohamed Beltagy, Manal Moustafa Abdel-Rahman, Hanaa Mahdy Abouzied, Samia Ahmed Madkour. Enhance Maize (Zea mays L.) Tolerance to Abiotic Stress Through the Genetic Transformation with Anthranilate Synthase (ASA2 Gene) Using Particle Bombardment. Am J BioSci Bioeng. 2019;7(2):28-33. doi: 10.11648/j.bio.20190702.11

Copy | Download

@article{10.11648/j.bio.20190702.11,
  author = {Karim Mohamed Beltagy and Manal Moustafa Abdel-Rahman and Hanaa Mahdy Abouzied and Samia Ahmed Madkour},
  title = {Enhance Maize (Zea mays L.) Tolerance to Abiotic Stress Through the Genetic Transformation with Anthranilate Synthase (ASA2 Gene) Using Particle Bombardment},
  journal = {American Journal of Bioscience and Bioengineering},
  volume = {7},
  number = {2},
  pages = {28-33},
  doi = {10.11648/j.bio.20190702.11},
  url = {https://doi.org/10.11648/j.bio.20190702.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bio.20190702.11},
  abstract = {The agricultural plans in Egypt aim to increase maize production and yield to reduce the importation and to meet the country's need from maize. Currently the crop production is affected by the climatic changes which rise the abiotic and biotic stresses problems. In an attempt to raise the tryptophan level in maize, particle bombardment technique was used with two hybrids maize, namely Sc168 and Sc10. The results of this study showed that both hybrids were transformed efficiently and showed increased levels of tryptophan in the two hybrids. The double shot 1100 psi was higher effective than single shot. It can be concluded that levels of tryptophan increased clearly in the two hybrids which is considered an indicator for ASA2 successful expressing as compared to control. The present study established effective tissue culture protocol for maize hybrids suitable for gene transformation which may used in maize improvement programs in the future.},
 year = {2019}
}

Copy | Download

TY  - JOUR
T1  - Enhance Maize (Zea mays L.) Tolerance to Abiotic Stress Through the Genetic Transformation with Anthranilate Synthase (ASA2 Gene) Using Particle Bombardment
AU  - Karim Mohamed Beltagy
AU  - Manal Moustafa Abdel-Rahman
AU  - Hanaa Mahdy Abouzied
AU  - Samia Ahmed Madkour
Y1  - 2019/05/07
PY  - 2019
N1  - https://doi.org/10.11648/j.bio.20190702.11
DO  - 10.11648/j.bio.20190702.11
T2  - American Journal of Bioscience and Bioengineering
JF  - American Journal of Bioscience and Bioengineering
JO  - American Journal of Bioscience and Bioengineering
SP  - 28
EP  - 33
PB  - Science Publishing Group
SN  - 2328-5893
UR  - https://doi.org/10.11648/j.bio.20190702.11
AB  - The agricultural plans in Egypt aim to increase maize production and yield to reduce the importation and to meet the country's need from maize. Currently the crop production is affected by the climatic changes which rise the abiotic and biotic stresses problems. In an attempt to raise the tryptophan level in maize, particle bombardment technique was used with two hybrids maize, namely Sc168 and Sc10. The results of this study showed that both hybrids were transformed efficiently and showed increased levels of tryptophan in the two hybrids. The double shot 1100 psi was higher effective than single shot. It can be concluded that levels of tryptophan increased clearly in the two hybrids which is considered an indicator for ASA2 successful expressing as compared to control. The present study established effective tissue culture protocol for maize hybrids suitable for gene transformation which may used in maize improvement programs in the future.
VL  - 7
IS  - 2
ER  -

Copy | Download