In Vitro Antibacterial Activities of Methanol and Aqueous Extracts of Leaves of <i>Carica papaya</i> and <i>Moringa oleifera</i> Against Selected Human Pathogenic Bacteria

Temam Gemeda Genemo

doi:doi:10.11648/j.bio.20210905.12

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In Vitro Antibacterial Activities of Methanol and Aqueous Extracts of Leaves of Carica papaya and Moringa oleifera Against Selected Human Pathogenic Bacteria

Temam Gemeda Genemo

Published in American Journal of Bioscience and Bioengineering (Volume 9, Issue 5)

Received: 30 August 2021 Accepted: 14 September 2021 Published: 23 September 2021

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Abstract

A medicinal plant is any plant which, in one or more of its organs, contains substances that can be used for therapeutic purposes. In developing countries all over the world, large numbers of people die daily of preventable or curable diseases because of the lack of even simple health care. So the study was interested entitled “In vitro antibacterial activities of methanol and aqueous extracts of leaves of Carica papaya and Moringa oleifera against selected human pathogenic bacteria”. Moringa oleifera and Carica papaya is medicinal plants which have medicinal values for the treatment of various infectious illness were interested for investigation of their antibacterial activities against E. coli and H. pylore. Two solvent types (methanol and distilled water) were used for crude extraction. The vulnerability of the pathogen to the antibacterial substances was determined using the disc diffusion method. Minimum Inhibitory Concentration was determined by the broth dilution method. The results of the antibacterial activities revealed that both methanol and aqueous leaf extracts had inhibitory activities against the selected gram-positive and gram-negative test pathogens. Methanol extract of Carica papaya had the highest antibacterial activity (13.3 mm) against H. pylore, while Moringa oleifera indica exhibited the least zone of inhibition (8.2 mm) at a concentration of 150 mg/mL. The Antibacterial activities of heat treated crude extracts against the test pathogens were also determined at varying temperature (45-55°C) for a period of 30 and 60 minutes. The results revealed that at higher temperature and exposure time, there was a decrease in the zone of inhibitions. The Minimum Inhibitory Concentrations of the methanol extracts ranged from 1.25 mg/ml - 5 mg/mL; whereas, for aqueous extracts ranged from 2.5 mg/mL -10 mg/mL. In general, this study provides base line information for further work on the search for specific active compounds from the selected plant leaf extracts against human pathogenic bacteria.

Published in	American Journal of Bioscience and Bioengineering (Volume 9, Issue 5)
DOI	10.11648/j.bio.20210905.12
Page(s)	136-142
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2021. Published by Science Publishing Group

Keywords

Antibacterial Activity, Carica papaya, E. coli, Disc Diffusion, Heat Treatment, H. pylore, Moringa oleifera

References

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[8]	Farooq, F., Rai, M., Tiwari, A., Khan, A. A. and Farooq, S. 2012. Medicinal properties of Moringa oleifera: An overview of promising healer. Journal of Medicinal Plants Research, 6 (27): 4368-4374.
[9]	Jayaraman, S., Manoharan, M. S. and Illanchezian, S., 2008. In-vitro antimicrobial and antitumor activities of Stevia rebaudiana (Asteraceae) leaf extracts. Tropical Journal of Pharmaceutical Research, 7 (4): 1143-1149.
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[14]	Shah, J. S., Shah, M. B., Goswami, S. S. and Santani, D. D. 2006. Mechanism of action of antiulcer activity of bark extracts of Manilkara hexandra against experimentally induced gastric ulcers in rats. Pharmacognosy Magazine, 5 (2): 41-45.
[15]	Simlai, A. and Roy, A. 2012. Analysis of and correlation between phytochemical and antimicrobial constituents of Ceriopsdecandra, a medicinal mangrove plant, from Indian Sundarbanestuary. Journal of Medicinal Plants Research, 6 (32): 4755-4765.
[16]	Taylor, J. L. S., Rabe, T., McGaw, L. J., Jäger, A. K. and Van Staden, J., 2001. Towards the scientific validation of traditional medicinal plants. Plant growth regulation, 34 (1): 23-37.
[17]	Thilza, I. B., Sanni, S., Zakari, A. I., Sanni, F. S., Muhammed, T. and Musa, B. J. 2010. In vitro antimicrobial activity of water extract of Moringa oleifera leaf stalk on bacteria normally implicated in eye diseases. Academia arena, 2 (6): 80-82.
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APA Style

Temam Gemeda Genemo. (2021). In Vitro Antibacterial Activities of Methanol and Aqueous Extracts of Leaves of Carica papaya and Moringa oleifera Against Selected Human Pathogenic Bacteria. American Journal of Bioscience and Bioengineering, 9(5), 136-142. https://doi.org/10.11648/j.bio.20210905.12

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ACS Style

Temam Gemeda Genemo. In Vitro Antibacterial Activities of Methanol and Aqueous Extracts of Leaves of Carica papaya and Moringa oleifera Against Selected Human Pathogenic Bacteria. Am. J. BioSci. Bioeng. 2021, 9(5), 136-142. doi: 10.11648/j.bio.20210905.12

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AMA Style

Temam Gemeda Genemo. In Vitro Antibacterial Activities of Methanol and Aqueous Extracts of Leaves of Carica papaya and Moringa oleifera Against Selected Human Pathogenic Bacteria. Am J BioSci Bioeng. 2021;9(5):136-142. doi: 10.11648/j.bio.20210905.12

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@article{10.11648/j.bio.20210905.12,
  author = {Temam Gemeda Genemo},
  title = {In Vitro Antibacterial Activities of Methanol and Aqueous Extracts of Leaves of Carica papaya and Moringa oleifera Against Selected Human Pathogenic Bacteria},
  journal = {American Journal of Bioscience and Bioengineering},
  volume = {9},
  number = {5},
  pages = {136-142},
  doi = {10.11648/j.bio.20210905.12},
  url = {https://doi.org/10.11648/j.bio.20210905.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bio.20210905.12},
  abstract = {A medicinal plant is any plant which, in one or more of its organs, contains substances that can be used for therapeutic purposes. In developing countries all over the world, large numbers of people die daily of preventable or curable diseases because of the lack of even simple health care. So the study was interested entitled “In vitro antibacterial activities of methanol and aqueous extracts of leaves of Carica papaya and Moringa oleifera against selected human pathogenic bacteria”. Moringa oleifera and Carica papaya is medicinal plants which have medicinal values for the treatment of various infectious illness were interested for investigation of their antibacterial activities against E. coli and H. pylore. Two solvent types (methanol and distilled water) were used for crude extraction. The vulnerability of the pathogen to the antibacterial substances was determined using the disc diffusion method. Minimum Inhibitory Concentration was determined by the broth dilution method. The results of the antibacterial activities revealed that both methanol and aqueous leaf extracts had inhibitory activities against the selected gram-positive and gram-negative test pathogens. Methanol extract of Carica papaya had the highest antibacterial activity (13.3 mm) against H. pylore, while Moringa oleifera indica exhibited the least zone of inhibition (8.2 mm) at a concentration of 150 mg/mL. The Antibacterial activities of heat treated crude extracts against the test pathogens were also determined at varying temperature (45-55°C) for a period of 30 and 60 minutes. The results revealed that at higher temperature and exposure time, there was a decrease in the zone of inhibitions. The Minimum Inhibitory Concentrations of the methanol extracts ranged from 1.25 mg/ml - 5 mg/mL; whereas, for aqueous extracts ranged from 2.5 mg/mL -10 mg/mL. In general, this study provides base line information for further work on the search for specific active compounds from the selected plant leaf extracts against human pathogenic bacteria.},
 year = {2021}
}

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TY - JOUR
T1 - In Vitro Antibacterial Activities of Methanol and Aqueous Extracts of Leaves of Carica papaya and Moringa oleifera Against Selected Human Pathogenic Bacteria
AU - Temam Gemeda Genemo
Y1 - 2021/09/23
PY - 2021
N1 - https://doi.org/10.11648/j.bio.20210905.12
DO - 10.11648/j.bio.20210905.12
T2 - American Journal of Bioscience and Bioengineering
JF - American Journal of Bioscience and Bioengineering
JO - American Journal of Bioscience and Bioengineering
SP - 136
EP - 142
PB - Science Publishing Group
SN - 2328-5893
UR - https://doi.org/10.11648/j.bio.20210905.12
AB - A medicinal plant is any plant which, in one or more of its organs, contains substances that can be used for therapeutic purposes. In developing countries all over the world, large numbers of people die daily of preventable or curable diseases because of the lack of even simple health care. So the study was interested entitled “In vitro antibacterial activities of methanol and aqueous extracts of leaves of Carica papaya and Moringa oleifera against selected human pathogenic bacteria”. Moringa oleifera and Carica papaya is medicinal plants which have medicinal values for the treatment of various infectious illness were interested for investigation of their antibacterial activities against E. coli and H. pylore. Two solvent types (methanol and distilled water) were used for crude extraction. The vulnerability of the pathogen to the antibacterial substances was determined using the disc diffusion method. Minimum Inhibitory Concentration was determined by the broth dilution method. The results of the antibacterial activities revealed that both methanol and aqueous leaf extracts had inhibitory activities against the selected gram-positive and gram-negative test pathogens. Methanol extract of Carica papaya had the highest antibacterial activity (13.3 mm) against H. pylore, while Moringa oleifera indica exhibited the least zone of inhibition (8.2 mm) at a concentration of 150 mg/mL. The Antibacterial activities of heat treated crude extracts against the test pathogens were also determined at varying temperature (45-55°C) for a period of 30 and 60 minutes. The results revealed that at higher temperature and exposure time, there was a decrease in the zone of inhibitions. The Minimum Inhibitory Concentrations of the methanol extracts ranged from 1.25 mg/ml - 5 mg/mL; whereas, for aqueous extracts ranged from 2.5 mg/mL -10 mg/mL. In general, this study provides base line information for further work on the search for specific active compounds from the selected plant leaf extracts against human pathogenic bacteria.
VL - 9
IS - 5
ER -

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Temam Gemeda Genemo

Department of Biotechnology, College of Natural and Computational Sciences, Wachemo University, Hossana, Ethiopia

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APA Style

Temam Gemeda Genemo. (2021). In Vitro Antibacterial Activities of Methanol and Aqueous Extracts of Leaves of Carica papaya and Moringa oleifera Against Selected Human Pathogenic Bacteria. American Journal of Bioscience and Bioengineering, 9(5), 136-142. https://doi.org/10.11648/j.bio.20210905.12

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ACS Style

Temam Gemeda Genemo. In Vitro Antibacterial Activities of Methanol and Aqueous Extracts of Leaves of Carica papaya and Moringa oleifera Against Selected Human Pathogenic Bacteria. Am. J. BioSci. Bioeng. 2021, 9(5), 136-142. doi: 10.11648/j.bio.20210905.12

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AMA Style

Temam Gemeda Genemo. In Vitro Antibacterial Activities of Methanol and Aqueous Extracts of Leaves of Carica papaya and Moringa oleifera Against Selected Human Pathogenic Bacteria. Am J BioSci Bioeng. 2021;9(5):136-142. doi: 10.11648/j.bio.20210905.12

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@article{10.11648/j.bio.20210905.12,
  author = {Temam Gemeda Genemo},
  title = {In Vitro Antibacterial Activities of Methanol and Aqueous Extracts of Leaves of Carica papaya and Moringa oleifera Against Selected Human Pathogenic Bacteria},
  journal = {American Journal of Bioscience and Bioengineering},
  volume = {9},
  number = {5},
  pages = {136-142},
  doi = {10.11648/j.bio.20210905.12},
  url = {https://doi.org/10.11648/j.bio.20210905.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bio.20210905.12},
  abstract = {A medicinal plant is any plant which, in one or more of its organs, contains substances that can be used for therapeutic purposes. In developing countries all over the world, large numbers of people die daily of preventable or curable diseases because of the lack of even simple health care. So the study was interested entitled “In vitro antibacterial activities of methanol and aqueous extracts of leaves of Carica papaya and Moringa oleifera against selected human pathogenic bacteria”. Moringa oleifera and Carica papaya is medicinal plants which have medicinal values for the treatment of various infectious illness were interested for investigation of their antibacterial activities against E. coli and H. pylore. Two solvent types (methanol and distilled water) were used for crude extraction. The vulnerability of the pathogen to the antibacterial substances was determined using the disc diffusion method. Minimum Inhibitory Concentration was determined by the broth dilution method. The results of the antibacterial activities revealed that both methanol and aqueous leaf extracts had inhibitory activities against the selected gram-positive and gram-negative test pathogens. Methanol extract of Carica papaya had the highest antibacterial activity (13.3 mm) against H. pylore, while Moringa oleifera indica exhibited the least zone of inhibition (8.2 mm) at a concentration of 150 mg/mL. The Antibacterial activities of heat treated crude extracts against the test pathogens were also determined at varying temperature (45-55°C) for a period of 30 and 60 minutes. The results revealed that at higher temperature and exposure time, there was a decrease in the zone of inhibitions. The Minimum Inhibitory Concentrations of the methanol extracts ranged from 1.25 mg/ml - 5 mg/mL; whereas, for aqueous extracts ranged from 2.5 mg/mL -10 mg/mL. In general, this study provides base line information for further work on the search for specific active compounds from the selected plant leaf extracts against human pathogenic bacteria.},
 year = {2021}
}

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