@article{10.11648/j.bio.20221003.12,
  author = {Abdullahi Yusuf Bello and Abubakar Muazu Jodi and Abubakar Sanusi and Ridwan Abdulsalam Hussein and Asabe Danjuma},
  title = {Studies of Lodge Bacteria on Lablab purpureau and Pennisetum hybridum for Potential Degradation of Polycyclic Aromatic Hydrocarbons},
  journal = {American Journal of Bioscience and Bioengineering},
  volume = {10},
  number = {3},
  pages = {48-52},
  doi = {10.11648/j.bio.20221003.12},
  url = {https://doi.org/10.11648/j.bio.20221003.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bio.20221003.12},
  abstract = {A plants tissue lodge bacteria that do not harm plants body but supports its living, these are endophytic bacteria. Studies on Pennisetum hybdridum and Lablab purpureau endophytic bacteria for degradation of Polyaromatic hydrocarbons (PAHs) were carried out. These bacteria were enumerated, isolated conventionally, screened and determine their degradation potential of diesel and kerosene (PAHs). The total culturable bacterial count on Lablab Purpureus (HyB) was 2.0×104 cfu/g and Pennisetum hybridum (KgG) 3.2×105 cfu/g wet plant. About nine (9) bacteria from Lablab purpureau and ten (10) bacteria from Pennisetum hybdridum were isolated by standard plate techniques. These bacteria were screened using minimal salt agar amended with varied concentrations of kerosene and diesel (PAHs). The bacteria KgG-1, Bacillus sp. presented sufficient growth on minimal salt agar plates at 0.8% diesel and 1.0% kerosene. Optimal growth was studied using glucose (0.2 to 1.0 % w/v) as energy and carbon source and cow urine (0.2 to 0.8% v/v) as nitrogen source. The conditions used for optimal growth determination were incubation temperature 36±1°C and incubation period 12 hours interval from 0 to 72 hours. The growth was measured using spectrophotometer 600 nm absorbance. The highest observed growth after addition of glucose (1.0% w/v) in the presence of diesel was 0.53 for a period of 60 hours. Similarly, maximal growth after added kerosene, 0.8% w/v glucose was 0.635 for 60 hours. The cow urine 0.4% v/v in the presence of diesel after 48 hours was maximal 0.20. Also, in the presence of kerosene, the highest growth was 0.235 at 0.4%v/v cow urine for 60 hours. The degradation by Bacillus sp was performed on minimal salt broth contained with 0.8% diesel, 1.0% kerosene per 1000 ml, 1.0% glucose w/v, 0.4% cow urine v/v added with 1 ml bacterial suspension. Control set does not carry bacterial suspension. Optical density was determined from 0 to 96 hours at an interval of 24 hours. For degradation of PAHs, the highest optical density for Bacillus sp. was 0.471 at 72 hours in the presence of kerosene while for diesel 0.532 at 96 hours. Increasing incubation period increases growth rate of Bacillus on PAHs.},
 year = {2022}
}

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TY  - JOUR
T1  - Studies of Lodge Bacteria on Lablab purpureau and Pennisetum hybridum for Potential Degradation of Polycyclic Aromatic Hydrocarbons
AU  - Abdullahi Yusuf Bello
AU  - Abubakar Muazu Jodi
AU  - Abubakar Sanusi
AU  - Ridwan Abdulsalam Hussein
AU  - Asabe Danjuma
Y1  - 2022/05/31
PY  - 2022
N1  - https://doi.org/10.11648/j.bio.20221003.12
DO  - 10.11648/j.bio.20221003.12
T2  - American Journal of Bioscience and Bioengineering
JF  - American Journal of Bioscience and Bioengineering
JO  - American Journal of Bioscience and Bioengineering
SP  - 48
EP  - 52
PB  - Science Publishing Group
SN  - 2328-5893
UR  - https://doi.org/10.11648/j.bio.20221003.12
AB  - A plants tissue lodge bacteria that do not harm plants body but supports its living, these are endophytic bacteria. Studies on Pennisetum hybdridum and Lablab purpureau endophytic bacteria for degradation of Polyaromatic hydrocarbons (PAHs) were carried out. These bacteria were enumerated, isolated conventionally, screened and determine their degradation potential of diesel and kerosene (PAHs). The total culturable bacterial count on Lablab Purpureus (HyB) was 2.0×104 cfu/g and Pennisetum hybridum (KgG) 3.2×105 cfu/g wet plant. About nine (9) bacteria from Lablab purpureau and ten (10) bacteria from Pennisetum hybdridum were isolated by standard plate techniques. These bacteria were screened using minimal salt agar amended with varied concentrations of kerosene and diesel (PAHs). The bacteria KgG-1, Bacillus sp. presented sufficient growth on minimal salt agar plates at 0.8% diesel and 1.0% kerosene. Optimal growth was studied using glucose (0.2 to 1.0 % w/v) as energy and carbon source and cow urine (0.2 to 0.8% v/v) as nitrogen source. The conditions used for optimal growth determination were incubation temperature 36±1°C and incubation period 12 hours interval from 0 to 72 hours. The growth was measured using spectrophotometer 600 nm absorbance. The highest observed growth after addition of glucose (1.0% w/v) in the presence of diesel was 0.53 for a period of 60 hours. Similarly, maximal growth after added kerosene, 0.8% w/v glucose was 0.635 for 60 hours. The cow urine 0.4% v/v in the presence of diesel after 48 hours was maximal 0.20. Also, in the presence of kerosene, the highest growth was 0.235 at 0.4%v/v cow urine for 60 hours. The degradation by Bacillus sp was performed on minimal salt broth contained with 0.8% diesel, 1.0% kerosene per 1000 ml, 1.0% glucose w/v, 0.4% cow urine v/v added with 1 ml bacterial suspension. Control set does not carry bacterial suspension. Optical density was determined from 0 to 96 hours at an interval of 24 hours. For degradation of PAHs, the highest optical density for Bacillus sp. was 0.471 at 72 hours in the presence of kerosene while for diesel 0.532 at 96 hours. Increasing incubation period increases growth rate of Bacillus on PAHs.
VL  - 10
IS  - 3
ER  - 

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