Perpetual Smoking Compromise the Osmotic Fragility of RBC

Mazhar Mushtaq; Uzma Jabbar; Mumtaz Begum; Naim Ahmad Nazami

doi:doi:10.11648/j.bs.20170303.11

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Perpetual Smoking Compromise the Osmotic Fragility of RBC

Mazhar Mushtaq, Uzma Jabbar, Mumtaz Begum, Naim Ahmad Nazami

Published in Biomedical Sciences (Volume 3, Issue 3)

Received: 16 May 2017 Accepted: 14 June 2017 Published: 21 July 2017

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Abstract

Hydrogen Peroxide (HP) is one of the Reactive Oxygen Species (ROS) causing cellular injury. The present study is aimed to assess the level of HP, catalase, total glutathione (T-GSH) in chronic smokers and compare these with acute smoker, passive smokers and non-smokers. In the peripheral blood of chronic smoker, acute smoker, passive smokers and control subjects, oxidative stress was measured in terms of HP present in the serum; antioxidant status was measured by blood T-GSH and catalase activity. Adverse effects of HP is measured in term of osmotic fragility of RBC. Increase amount of HP in serum of smoker was observed, whereas two fold increase of the HP was detected in the serum of acute smoker and moderate amount was detected in passive smoker as compare to non-smoker. Variability in the measurement of catalase and T-GSH were also significantly evident in the groups. Increase concentration of HP in acute smoker suggested that ROS produced in smoking not only have their adverse effects in the lungs but they can cross the alveolar epithelium. Osmotic fragility test indicated the increase in fragility of RBC membrane of smoker when they are subjected to high concentration of HP in vitro.

Published in	Biomedical Sciences (Volume 3, Issue 3)
DOI	10.11648/j.bs.20170303.11
Page(s)	58-62
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2017. Published by Science Publishing Group

Keywords

Hydrogen Peroxide, Reactive Oxygen Species, Oxidative Stress, Catalase, Glutathione, Smoker

References

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APA Style

Mazhar Mushtaq, Uzma Jabbar, Mumtaz Begum, Naim Ahmad Nazami. (2017). Perpetual Smoking Compromise the Osmotic Fragility of RBC. Biomedical Sciences, 3(3), 58-62. https://doi.org/10.11648/j.bs.20170303.11

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ACS Style

Mazhar Mushtaq; Uzma Jabbar; Mumtaz Begum; Naim Ahmad Nazami. Perpetual Smoking Compromise the Osmotic Fragility of RBC. Biomed. Sci. 2017, 3(3), 58-62. doi: 10.11648/j.bs.20170303.11

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AMA Style

Mazhar Mushtaq, Uzma Jabbar, Mumtaz Begum, Naim Ahmad Nazami. Perpetual Smoking Compromise the Osmotic Fragility of RBC. Biomed Sci. 2017;3(3):58-62. doi: 10.11648/j.bs.20170303.11

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@article{10.11648/j.bs.20170303.11,
  author = {Mazhar Mushtaq and Uzma Jabbar and Mumtaz Begum and Naim Ahmad Nazami},
  title = {Perpetual Smoking Compromise the Osmotic Fragility of RBC},
  journal = {Biomedical Sciences},
  volume = {3},
  number = {3},
  pages = {58-62},
  doi = {10.11648/j.bs.20170303.11},
  url = {https://doi.org/10.11648/j.bs.20170303.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bs.20170303.11},
  abstract = {Hydrogen Peroxide (HP) is one of the Reactive Oxygen Species (ROS) causing cellular injury. The present study is aimed to assess the level of HP, catalase, total glutathione (T-GSH) in chronic smokers and compare these with acute smoker, passive smokers and non-smokers. In the peripheral blood of chronic smoker, acute smoker, passive smokers and control subjects, oxidative stress was measured in terms of HP present in the serum; antioxidant status was measured by blood T-GSH and catalase activity. Adverse effects of HP is measured in term of osmotic fragility of RBC. Increase amount of HP in serum of smoker was observed, whereas two fold increase of the HP was detected in the serum of acute smoker and moderate amount was detected in passive smoker as compare to non-smoker. Variability in the measurement of catalase and T-GSH were also significantly evident in the groups. Increase concentration of HP in acute smoker suggested that ROS produced in smoking not only have their adverse effects in the lungs but they can cross the alveolar epithelium. Osmotic fragility test indicated the increase in fragility of RBC membrane of smoker when they are subjected to high concentration of HP in vitro.},
 year = {2017}
}

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TY  - JOUR
T1  - Perpetual Smoking Compromise the Osmotic Fragility of RBC
AU  - Mazhar Mushtaq
AU  - Uzma Jabbar
AU  - Mumtaz Begum
AU  - Naim Ahmad Nazami
Y1  - 2017/07/21
PY  - 2017
N1  - https://doi.org/10.11648/j.bs.20170303.11
DO  - 10.11648/j.bs.20170303.11
T2  - Biomedical Sciences
JF  - Biomedical Sciences
JO  - Biomedical Sciences
SP  - 58
EP  - 62
PB  - Science Publishing Group
SN  - 2575-3932
UR  - https://doi.org/10.11648/j.bs.20170303.11
AB  - Hydrogen Peroxide (HP) is one of the Reactive Oxygen Species (ROS) causing cellular injury. The present study is aimed to assess the level of HP, catalase, total glutathione (T-GSH) in chronic smokers and compare these with acute smoker, passive smokers and non-smokers. In the peripheral blood of chronic smoker, acute smoker, passive smokers and control subjects, oxidative stress was measured in terms of HP present in the serum; antioxidant status was measured by blood T-GSH and catalase activity. Adverse effects of HP is measured in term of osmotic fragility of RBC. Increase amount of HP in serum of smoker was observed, whereas two fold increase of the HP was detected in the serum of acute smoker and moderate amount was detected in passive smoker as compare to non-smoker. Variability in the measurement of catalase and T-GSH were also significantly evident in the groups. Increase concentration of HP in acute smoker suggested that ROS produced in smoking not only have their adverse effects in the lungs but they can cross the alveolar epithelium. Osmotic fragility test indicated the increase in fragility of RBC membrane of smoker when they are subjected to high concentration of HP in vitro.
VL  - 3
IS  - 3
ER  -

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Author Information

Mazhar Mushtaq

Biochemistry Department, Shalamar Medical and Dental College, Affiliated with University of Health Sciences, Lahore, Pakistan
Uzma Jabbar

Biochemistry Department, Shalamar Medical and Dental College, Affiliated with University of Health Sciences, Lahore, Pakistan
Mumtaz Begum

Biochemistry Department, Shalamar Medical and Dental College, Affiliated with University of Health Sciences, Lahore, Pakistan
Naim Ahmad Nazami

Biochemistry Department, Shalamar Medical and Dental College, Affiliated with University of Health Sciences, Lahore, Pakistan

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Plain Text BibTeX RIS

APA Style

Mazhar Mushtaq, Uzma Jabbar, Mumtaz Begum, Naim Ahmad Nazami. (2017). Perpetual Smoking Compromise the Osmotic Fragility of RBC. Biomedical Sciences, 3(3), 58-62. https://doi.org/10.11648/j.bs.20170303.11

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ACS Style

Mazhar Mushtaq; Uzma Jabbar; Mumtaz Begum; Naim Ahmad Nazami. Perpetual Smoking Compromise the Osmotic Fragility of RBC. Biomed. Sci. 2017, 3(3), 58-62. doi: 10.11648/j.bs.20170303.11

Copy | Download

AMA Style

Mazhar Mushtaq, Uzma Jabbar, Mumtaz Begum, Naim Ahmad Nazami. Perpetual Smoking Compromise the Osmotic Fragility of RBC. Biomed Sci. 2017;3(3):58-62. doi: 10.11648/j.bs.20170303.11

Copy | Download

@article{10.11648/j.bs.20170303.11,
  author = {Mazhar Mushtaq and Uzma Jabbar and Mumtaz Begum and Naim Ahmad Nazami},
  title = {Perpetual Smoking Compromise the Osmotic Fragility of RBC},
  journal = {Biomedical Sciences},
  volume = {3},
  number = {3},
  pages = {58-62},
  doi = {10.11648/j.bs.20170303.11},
  url = {https://doi.org/10.11648/j.bs.20170303.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bs.20170303.11},
  abstract = {Hydrogen Peroxide (HP) is one of the Reactive Oxygen Species (ROS) causing cellular injury. The present study is aimed to assess the level of HP, catalase, total glutathione (T-GSH) in chronic smokers and compare these with acute smoker, passive smokers and non-smokers. In the peripheral blood of chronic smoker, acute smoker, passive smokers and control subjects, oxidative stress was measured in terms of HP present in the serum; antioxidant status was measured by blood T-GSH and catalase activity. Adverse effects of HP is measured in term of osmotic fragility of RBC. Increase amount of HP in serum of smoker was observed, whereas two fold increase of the HP was detected in the serum of acute smoker and moderate amount was detected in passive smoker as compare to non-smoker. Variability in the measurement of catalase and T-GSH were also significantly evident in the groups. Increase concentration of HP in acute smoker suggested that ROS produced in smoking not only have their adverse effects in the lungs but they can cross the alveolar epithelium. Osmotic fragility test indicated the increase in fragility of RBC membrane of smoker when they are subjected to high concentration of HP in vitro.},
 year = {2017}
}

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TY  - JOUR
T1  - Perpetual Smoking Compromise the Osmotic Fragility of RBC
AU  - Mazhar Mushtaq
AU  - Uzma Jabbar
AU  - Mumtaz Begum
AU  - Naim Ahmad Nazami
Y1  - 2017/07/21
PY  - 2017
N1  - https://doi.org/10.11648/j.bs.20170303.11
DO  - 10.11648/j.bs.20170303.11
T2  - Biomedical Sciences
JF  - Biomedical Sciences
JO  - Biomedical Sciences
SP  - 58
EP  - 62
PB  - Science Publishing Group
SN  - 2575-3932
UR  - https://doi.org/10.11648/j.bs.20170303.11
AB  - Hydrogen Peroxide (HP) is one of the Reactive Oxygen Species (ROS) causing cellular injury. The present study is aimed to assess the level of HP, catalase, total glutathione (T-GSH) in chronic smokers and compare these with acute smoker, passive smokers and non-smokers. In the peripheral blood of chronic smoker, acute smoker, passive smokers and control subjects, oxidative stress was measured in terms of HP present in the serum; antioxidant status was measured by blood T-GSH and catalase activity. Adverse effects of HP is measured in term of osmotic fragility of RBC. Increase amount of HP in serum of smoker was observed, whereas two fold increase of the HP was detected in the serum of acute smoker and moderate amount was detected in passive smoker as compare to non-smoker. Variability in the measurement of catalase and T-GSH were also significantly evident in the groups. Increase concentration of HP in acute smoker suggested that ROS produced in smoking not only have their adverse effects in the lungs but they can cross the alveolar epithelium. Osmotic fragility test indicated the increase in fragility of RBC membrane of smoker when they are subjected to high concentration of HP in vitro.
VL  - 3
IS  - 3
ER  -

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