Study of G6pd Deficit (Glucose-6-phosphate Deshydrogenase) in Blood Donors in Brazzaville

M’pene Bel Varret; Fouti Gudrid Ludmilhia; Arsène Bikoue; Ongouda Brunel; Nguimbi Etienne

doi:doi:10.11648/j.bs.20200604.12

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Study of G6pd Deficit (Glucose-6-phosphate Deshydrogenase) in Blood Donors in Brazzaville

M’pene Bel Varret, Fouti Gudrid Ludmilhia, Arsène Bikoue, Ongouda Brunel, Nguimbi Etienne

Published in Biomedical Sciences (Volume 6, Issue 4)

Received: 29 September 2020 Accepted: 17 October 2020 Published: 23 October 2020

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Abstract

Glucose-6-Phosphate dehydrogenase (G6PD) is an enzyme present in the cytoplasm of all cells in the body and protects red blood cells from certain attacks. The anomaly is genetically inherited in a recessive manner, linked to the X chromosome. Currently, an estimated 420 million individuals lack G6PD worldwide and its incidence varies between 1-25% in some African populations. This was an analytical cross-sectional study with prospective data collection that took place from May to November 2018, i.e. six (6) months. Our study included 108 blood donors, 88 men and 20 women. The samples were stored at 2-8 ° C for 24 hours while awaiting handling. The determination of the enzymatic activity of G6PD was carried out by the spectrophotometric method using a commercially available kit. 02/108 subjects studied (ie a prevalence of 1.85%) were deficient in our study. These two deficit were mostly young and between 18-25 years old only male. They were originally from the plateau and observed in the blood transfusion station (PTS) of the Talangai referral hospital. The mean hemoglobin value in normal subjects was 12.53 g / dl in men and 11.3 g / dl in women. In contrast, the average hemoglobin value was 10.45 g / dl in the deficient patients. The mean value of G6PD enzyme activity in normal subjects was 11.7 IU / g hemoglobin in men, 11.54 IU / g hemoglobin in women and in deficient subjects was 2.83 IU / g hemoglobin. As the results of our study show, G6PD deficiency affects hemoglobin levels.

Published in	Biomedical Sciences (Volume 6, Issue 4)
DOI	10.11648/j.bs.20200604.12
Page(s)	84-88
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2020. Published by Science Publishing Group

Keywords

Prevalence, G6PD Deficit, Blood Donors, Congo Brazzaville

References

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[2]	Wajcman H et al. Déficits en glucose-6-phosphate-déshydrogénase. EMC – Hematology 2006; 13-006-D-10, 9 p.
[3]	Yamoul M (2016). Anémie hémolytique par déficit en G6PD chez l’enfant. Université Mohamme V.
[4]	Salvador A, Savageau MA. Quantitative evolutionary design of glucose 6-phosphate dehydrogenase expression in human erythrocytes. Proc Natl Acad Sci U S A 2003; 100: 14463—8.
[5]	SARAH A et al (2001). Haplotype diversity and linkage Desiquilibrium at Human Glucose 6-phosphate dehydrogenasse: Recent origin of alleles that confer malaria resistance. Science, July 20, vol. 293, Issue 55229; 455-462.
[6]	SRIDEVI SUKUMAR et al (2002). Glucose 6- phosphate dehydrogenase gene mutation in India productin drug-induced haemolytic anemia, Bristish Journal of Haemtology, 116, 671-672.
[7]	Owen, R., Karl Landsteiner and the first human marker locus. Genetics, 2000. 155 (3): p. 995-8.
[8]	LUCIO LUZZATTO et al (2006). Glucose 6-phosphate dehydrgenase deficiency: From genotype to phenotype. Haematological/ the haematology journal/, 91 (10)/ 1303-1306.
[9]	AMADOU DIAWARA et al (2005). Déficit en G6PD chez les donneurs de sang du C. N. T. S. de Bamako. Th: Ph: Bamako,.-36p. 55.
[10]	Aubry (2014), Deficits enzymatiques hereditaires du globule rouge.
[11]	Estelle Noêla HV (2004). Prévalence du déficit en glucose-6-phosphate déshydrogénase (G6PD) et de l’association drépanocytose et déficit en g6pd chez les nouveau-nés dans la ville de Ouagadougou (Burkina-Faso). Université de Ouagadougou.
[12]	Mégarbane (2008)., Déficit en glucose-6-phosphate déshydrogénase: quand y penser et quelles précautions prendre ? Glucose-6-phosphate dehydrogenase deficiency. B. P,; 17 (4): 399-406.
[13]	Guitteye H et al (2003). La Sélection du donneur de sang par un dosage pré-don de l’hémoglobine. Thèse pharmacie Bamako n° 48.
[14]	Traore Oumou et al (2002). Phénotype érythrocytaires dans les systèmes de groupes sanguins immunogènes chez les donneurs de sang de Bamako. Thèse pharmacie Bamako.
[15]	Olivier MUKUKU et al (2016). Profil é épidémiologique et séroprévalence des donneurs de sang aux cliniques universitaire de Lubumbashi, République démocratique du Congo. Panafrican Medical Journal P: 4.
[16]	ADU et al (2016). Deficit en G6PD. Déficit En Glucose-6-Phosphate Déshydrogénase Et Faucille Trait parmi les donneurs de sang potentiels: une étude transversale à Berekum, Ghana. P: 7.
[17]	Akanni et al (2010). Deficit G6PD. Déficit en glucose-6-phosphate déshydrogénase chez des donneurs de sang et des nouveau-nés atteints de jaunisse à Osogbo, Nigeria. P: 1-4.
[18]	Beutler E et al. Hematologically important mutation: Glucose-6-phosphate deshydrogenase. Blood cells Mol Dis. 2002-Mar-Apr, 28 (2): 93-103.
[19]	HUSSEN et al (2011). Prevalence of glucose-6-phosphate-deshydrogenase (G6PD) deficiency (Favism) in Thamar province- Republique of yemen. P: 3 (1), 12-19.

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M’pene Bel Varret, Fouti Gudrid Ludmilhia, Arsène Bikoue, Ongouda Brunel, Nguimbi Etienne. (2020). Study of G6pd Deficit (Glucose-6-phosphate Deshydrogenase) in Blood Donors in Brazzaville. Biomedical Sciences, 6(4), 84-88. https://doi.org/10.11648/j.bs.20200604.12

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ACS Style

M’pene Bel Varret; Fouti Gudrid Ludmilhia; Arsène Bikoue; Ongouda Brunel; Nguimbi Etienne. Study of G6pd Deficit (Glucose-6-phosphate Deshydrogenase) in Blood Donors in Brazzaville. Biomed. Sci. 2020, 6(4), 84-88. doi: 10.11648/j.bs.20200604.12

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AMA Style

M’pene Bel Varret, Fouti Gudrid Ludmilhia, Arsène Bikoue, Ongouda Brunel, Nguimbi Etienne. Study of G6pd Deficit (Glucose-6-phosphate Deshydrogenase) in Blood Donors in Brazzaville. Biomed Sci. 2020;6(4):84-88. doi: 10.11648/j.bs.20200604.12

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@article{10.11648/j.bs.20200604.12,
  author = {M’pene Bel Varret and Fouti Gudrid Ludmilhia and Arsène Bikoue and Ongouda Brunel and Nguimbi Etienne},
  title = {Study of G6pd Deficit (Glucose-6-phosphate Deshydrogenase) in Blood Donors in Brazzaville},
  journal = {Biomedical Sciences},
  volume = {6},
  number = {4},
  pages = {84-88},
  doi = {10.11648/j.bs.20200604.12},
  url = {https://doi.org/10.11648/j.bs.20200604.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bs.20200604.12},
  abstract = {Glucose-6-Phosphate dehydrogenase (G6PD) is an enzyme present in the cytoplasm of all cells in the body and protects red blood cells from certain attacks. The anomaly is genetically inherited in a recessive manner, linked to the X chromosome. Currently, an estimated 420 million individuals lack G6PD worldwide and its incidence varies between 1-25% in some African populations. This was an analytical cross-sectional study with prospective data collection that took place from May to November 2018, i.e. six (6) months. Our study included 108 blood donors, 88 men and 20 women. The samples were stored at 2-8 ° C for 24 hours while awaiting handling. The determination of the enzymatic activity of G6PD was carried out by the spectrophotometric method using a commercially available kit. 02/108 subjects studied (ie a prevalence of 1.85%) were deficient in our study. These two deficit were mostly young and between 18-25 years old only male. They were originally from the plateau and observed in the blood transfusion station (PTS) of the Talangai referral hospital. The mean hemoglobin value in normal subjects was 12.53 g / dl in men and 11.3 g / dl in women. In contrast, the average hemoglobin value was 10.45 g / dl in the deficient patients. The mean value of G6PD enzyme activity in normal subjects was 11.7 IU / g hemoglobin in men, 11.54 IU / g hemoglobin in women and in deficient subjects was 2.83 IU / g hemoglobin. As the results of our study show, G6PD deficiency affects hemoglobin levels.},
 year = {2020}
}

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TY - JOUR
T1 - Study of G6pd Deficit (Glucose-6-phosphate Deshydrogenase) in Blood Donors in Brazzaville
AU - M’pene Bel Varret
AU - Fouti Gudrid Ludmilhia
AU - Arsène Bikoue
AU - Ongouda Brunel
AU - Nguimbi Etienne
Y1 - 2020/10/23
PY - 2020
N1 - https://doi.org/10.11648/j.bs.20200604.12
DO - 10.11648/j.bs.20200604.12
T2 - Biomedical Sciences
JF - Biomedical Sciences
JO - Biomedical Sciences
SP - 84
EP - 88
PB - Science Publishing Group
SN - 2575-3932
UR - https://doi.org/10.11648/j.bs.20200604.12
AB - Glucose-6-Phosphate dehydrogenase (G6PD) is an enzyme present in the cytoplasm of all cells in the body and protects red blood cells from certain attacks. The anomaly is genetically inherited in a recessive manner, linked to the X chromosome. Currently, an estimated 420 million individuals lack G6PD worldwide and its incidence varies between 1-25% in some African populations. This was an analytical cross-sectional study with prospective data collection that took place from May to November 2018, i.e. six (6) months. Our study included 108 blood donors, 88 men and 20 women. The samples were stored at 2-8 ° C for 24 hours while awaiting handling. The determination of the enzymatic activity of G6PD was carried out by the spectrophotometric method using a commercially available kit. 02/108 subjects studied (ie a prevalence of 1.85%) were deficient in our study. These two deficit were mostly young and between 18-25 years old only male. They were originally from the plateau and observed in the blood transfusion station (PTS) of the Talangai referral hospital. The mean hemoglobin value in normal subjects was 12.53 g / dl in men and 11.3 g / dl in women. In contrast, the average hemoglobin value was 10.45 g / dl in the deficient patients. The mean value of G6PD enzyme activity in normal subjects was 11.7 IU / g hemoglobin in men, 11.54 IU / g hemoglobin in women and in deficient subjects was 2.83 IU / g hemoglobin. As the results of our study show, G6PD deficiency affects hemoglobin levels.
VL - 6
IS - 4
ER -

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Author Information

M’pene Bel Varret

Unit of Biochemistry and Molecular Biology, Faculty of Health Sciences, University Marien Ngouabi, Brazzaville, Congo
Fouti Gudrid Ludmilhia

Hematology Laboratory, Faculty of Health Sciences, University Marien Ngouabi, Brazzaville, Congo
Arsène Bikoue

Immunohematology Laboratory, National Blood Transfusion Center, Brazzaville, Congo
Ongouda Brunel

Immunohematology Laboratory, National Blood Transfusion Center, Brazzaville, Congo
Nguimbi Etienne

Cellular and Molecular Biology Laboratory, Faculty of Technical Sciences, University Marien Ngouabi, Brazzaville, Congo

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APA Style

M’pene Bel Varret, Fouti Gudrid Ludmilhia, Arsène Bikoue, Ongouda Brunel, Nguimbi Etienne. (2020). Study of G6pd Deficit (Glucose-6-phosphate Deshydrogenase) in Blood Donors in Brazzaville. Biomedical Sciences, 6(4), 84-88. https://doi.org/10.11648/j.bs.20200604.12

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ACS Style

M’pene Bel Varret; Fouti Gudrid Ludmilhia; Arsène Bikoue; Ongouda Brunel; Nguimbi Etienne. Study of G6pd Deficit (Glucose-6-phosphate Deshydrogenase) in Blood Donors in Brazzaville. Biomed. Sci. 2020, 6(4), 84-88. doi: 10.11648/j.bs.20200604.12

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AMA Style

M’pene Bel Varret, Fouti Gudrid Ludmilhia, Arsène Bikoue, Ongouda Brunel, Nguimbi Etienne. Study of G6pd Deficit (Glucose-6-phosphate Deshydrogenase) in Blood Donors in Brazzaville. Biomed Sci. 2020;6(4):84-88. doi: 10.11648/j.bs.20200604.12

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@article{10.11648/j.bs.20200604.12,
  author = {M’pene Bel Varret and Fouti Gudrid Ludmilhia and Arsène Bikoue and Ongouda Brunel and Nguimbi Etienne},
  title = {Study of G6pd Deficit (Glucose-6-phosphate Deshydrogenase) in Blood Donors in Brazzaville},
  journal = {Biomedical Sciences},
  volume = {6},
  number = {4},
  pages = {84-88},
  doi = {10.11648/j.bs.20200604.12},
  url = {https://doi.org/10.11648/j.bs.20200604.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bs.20200604.12},
  abstract = {Glucose-6-Phosphate dehydrogenase (G6PD) is an enzyme present in the cytoplasm of all cells in the body and protects red blood cells from certain attacks. The anomaly is genetically inherited in a recessive manner, linked to the X chromosome. Currently, an estimated 420 million individuals lack G6PD worldwide and its incidence varies between 1-25% in some African populations. This was an analytical cross-sectional study with prospective data collection that took place from May to November 2018, i.e. six (6) months. Our study included 108 blood donors, 88 men and 20 women. The samples were stored at 2-8 ° C for 24 hours while awaiting handling. The determination of the enzymatic activity of G6PD was carried out by the spectrophotometric method using a commercially available kit. 02/108 subjects studied (ie a prevalence of 1.85%) were deficient in our study. These two deficit were mostly young and between 18-25 years old only male. They were originally from the plateau and observed in the blood transfusion station (PTS) of the Talangai referral hospital. The mean hemoglobin value in normal subjects was 12.53 g / dl in men and 11.3 g / dl in women. In contrast, the average hemoglobin value was 10.45 g / dl in the deficient patients. The mean value of G6PD enzyme activity in normal subjects was 11.7 IU / g hemoglobin in men, 11.54 IU / g hemoglobin in women and in deficient subjects was 2.83 IU / g hemoglobin. As the results of our study show, G6PD deficiency affects hemoglobin levels.},
 year = {2020}
}

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