Effects of Macamide B on the Expression of SIRT1 in Brain Tissue of Neonatal Mice with Hypoxic-ischemic Brain Damage

Ke Niu; Fengjie Xu; Guangyong Wang; Manhong Zeng

doi:doi:10.11648/j.bs.20210703.11

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Effects of Macamide B on the Expression of SIRT1 in Brain Tissue of Neonatal Mice with Hypoxic-ischemic Brain Damage

Ke Niu, Fengjie Xu, Guangyong Wang, Manhong Zeng

Published in Biomedical Sciences (Volume 7, Issue 3)

Received: 2 June 2021 Accepted: 19 June 2021 Published: 15 July 2021

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Abstract

Neonatal hypoxic-ischemic brain damage (HIBD) is the main cause of severe neurological diseases and death in newborns. Macamide B is an effective monomer extracted from Maca (Lepidium meyenii Walpers), which has important biological activities such as neuroprotection and neuromodulation. The purpose of this study was to investigate whether Macamide B can exert neuroprotective effects on HIBD in newborn mice by regulating Silent information regulator factor 2-related enzyme 1 (SIRT1). A modified Rice-Vannucci method was used to construct the HIBD model of newborn mice. The pups were divided into the following groups: sham group, HI group, and Macamide B group. On the first and third days after hypoxic-ischemic (HI), immunofluorescence and Western blot experiments were used to detect the expression level of SIRT1 in the brain tissue of infants. The results of the immunofluorescence experiment showed that compared with the sham group, the expression level of SIRT1 is significantly decreased in HI group pups, while the expression level of SIRT1 in pups pretreated with Macamide B increased significantly. The results of Western blot experiments are consistent with the results of immunofluorescence experiments. Our data indicate that Macamide B may exert a neuroprotective effect on HIBD in newborn mice by up-regulating the expression of SIRT1. Macamide B may become a new medicine effective in preventing and treating HIBD.

Published in	Biomedical Sciences (Volume 7, Issue 3)
DOI	10.11648/j.bs.20210703.11
Page(s)	64-68
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2021. Published by Science Publishing Group

Keywords

Macamide B, SIRT1, Neonatal Hypoxic-ischemic Brain Damage

References

[1]	Papazian O. Neonatal hypoxic-ischemic encephalopathy [J]. Medicina, 2018, 782: 36-41.
[2]	Nelson KB. Lynch JK. Stroke in newborn infants [J]. The Lancet Neurology, 2004, 3 (3): 150-158.
[3]	Nelson KB. Perinatal ischemic stroke [J]. Stroke, 2007, 38 (2 Suppl): 742-745.
[4]	Perlman JM. Intrapartum hypoxic-ischemic cerebral injury and subsequent cerebral palsy: medicolegal issues [J]. Pediatrics, 1997, 99 (6): 851-859.
[5]	Volpe JJ Neonatal encephalopathy: an inadequate term for hypoxic-ischemic encephalopathy [J]. Annals of neurology, 2012, 72 (2): 156-166.
[6]	Wassink G, Davidson JO, Dhillon SK, et al. Therapeutic Hypothermia in Neonatal Hypoxic-Ischemic Encephalopathy [J]. Current neurology and neuroscience reports, 2019, 19 (2): 2.
[7]	Michán S, Li Y, Chou MM, et al. SIRT1 is essential for normal cognitive function and synaptic plasticity [J]. The Journal of neuroscience, 2010, 30 (29): 9695-9707.
[8]	Ramadori G, Lee CE, Bookout AL, et al. Brain SIRT1: anatomical distribution and regulation by energy availability [J]. Neurosci, 2008, 28 (40): 9989-9996.
[9]	Meng X, Tan J, Li M, et al. Sirt1: Role Under the Condition of Ischemia/Hypoxia [J]. Cellular and molecular neurobiology, 2017, 37 (1): 17-28.
[10]	Muhammad I, Zhao J, Dunbar DC, et al. Constituents of Lepidium meyenii 'maca' [J]. Phytochemistry, 2002, 59 (1): 105-110.
[11]	Morris KC, Lin HW, Thompson JW, et al. Pathways for ischemic cytoprotection: role of sirtuins in caloric restriction [J], Cereb Blood Flow Metab, 2011, 31 (4): 1003-1019.
[12]	Gan L, Mucke L. Paths of convergence: sirtuins in aging and neurodegeneration [J]. Neuron, 2008, 58 (1): 10-14.
[13]	Gonzales GF. Ethnobiology and Ethnopharmacology of Lepidium meyenii (Maca), a Plant from the Peruvian Highlands [J]. Evidence-based complementary and alternative medicine, 2012, 2012: 193496.
[14]	Piacente S, Carbone V, Plaza A, et al. Investigation of the tuber constituents of maca (Lepidium meyenii Walp.) [J]. Journal of agricultural and food chemistry, 2002, 50 (20): 5621-5625.
[15]	Yali Wang YW, Brian McNeil, Linda M, et al. Harvey, Maca: An Andean crop with multi-pharmacological functions [J]. Food Research International, 2007, 40 (7): 783-792.
[16]	Ganzera M, Zhao J, Muhammad I, et al. Chemical profiling and standardization of Lepidium meyenii (Maca) by reversed phase high performance liquid chromatography [J]. Chemical & pharmaceutical bulletin, 2002, 50 (7): 988-991.
[17]	Uchiyama F, Jikyo T, Takeda R, et al. Lepidium meyenii (Maca) enhances the serum levels of luteinising hormone in female rats [J]. Journal of ethnopharmacology, 2014, 151 (2): 897-902.
[18]	McCollom MM, Villinski JR, McPhail KL, et al. Analysis of macamides in samples of Maca (Lepidium meyenii) by HPLC-UV-MS/MS [J]. Phytochemical analysis: PCA, 2005, 16 (6): 463-469.
[19]	Jiang W, Guo M, Gong M, et al. Vitamin A bio-modulates apoptosis via the mitochondrial pathway after hypoxic-ischemic brain damage [J]. Molecular brain, 2018, 11 (1): 14.
[20]	Rahman S, Islam R Mammalian. Sirt1: insights on its biological functions [J]. Cell communication and signaling: CCS, 2011, 9: 11.
[21]	Zakhary SM, Ayubcha D, Dileo JN, et al. Distribution analysis of deacetylase SIRT1 in rodent and human nervous systems [J]. Anatomical record (Hoboken, NJ: 2007), 2010, 293 (6): 1024-1032.
[22]	Howitz KT, Bitterman KJ, Cohen HY, et al. Small molecule activators of sirtuins extend Saccharomyces cerevisiae lifespan [J]. Nature, 2003, 425 (6954): 191-196.
[23]	Ji K, Sun X, Liu Y, et al. Regulation of Apoptosis and Radiation Sensitization in Lung Cancer Cells via the Sirt1/NF-κB/Smac Pathway [J]. Cell Physiol Biochem, 2018, 48 (1): 304-316.

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APA Style

Ke Niu, Fengjie Xu, Guangyong Wang, Manhong Zeng. (2021). Effects of Macamide B on the Expression of SIRT1 in Brain Tissue of Neonatal Mice with Hypoxic-ischemic Brain Damage. Biomedical Sciences, 7(3), 64-68. https://doi.org/10.11648/j.bs.20210703.11

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ACS Style

Ke Niu; Fengjie Xu; Guangyong Wang; Manhong Zeng. Effects of Macamide B on the Expression of SIRT1 in Brain Tissue of Neonatal Mice with Hypoxic-ischemic Brain Damage. Biomed. Sci. 2021, 7(3), 64-68. doi: 10.11648/j.bs.20210703.11

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AMA Style

Ke Niu, Fengjie Xu, Guangyong Wang, Manhong Zeng. Effects of Macamide B on the Expression of SIRT1 in Brain Tissue of Neonatal Mice with Hypoxic-ischemic Brain Damage. Biomed Sci. 2021;7(3):64-68. doi: 10.11648/j.bs.20210703.11

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@article{10.11648/j.bs.20210703.11,
  author = {Ke Niu and Fengjie Xu and Guangyong Wang and Manhong Zeng},
  title = {Effects of Macamide B on the Expression of SIRT1 in Brain Tissue of Neonatal Mice with Hypoxic-ischemic Brain Damage},
  journal = {Biomedical Sciences},
  volume = {7},
  number = {3},
  pages = {64-68},
  doi = {10.11648/j.bs.20210703.11},
  url = {https://doi.org/10.11648/j.bs.20210703.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bs.20210703.11},
  abstract = {Neonatal hypoxic-ischemic brain damage (HIBD) is the main cause of severe neurological diseases and death in newborns. Macamide B is an effective monomer extracted from Maca (Lepidium meyenii Walpers), which has important biological activities such as neuroprotection and neuromodulation. The purpose of this study was to investigate whether Macamide B can exert neuroprotective effects on HIBD in newborn mice by regulating Silent information regulator factor 2-related enzyme 1 (SIRT1). A modified Rice-Vannucci method was used to construct the HIBD model of newborn mice. The pups were divided into the following groups: sham group, HI group, and Macamide B group. On the first and third days after hypoxic-ischemic (HI), immunofluorescence and Western blot experiments were used to detect the expression level of SIRT1 in the brain tissue of infants. The results of the immunofluorescence experiment showed that compared with the sham group, the expression level of SIRT1 is significantly decreased in HI group pups, while the expression level of SIRT1 in pups pretreated with Macamide B increased significantly. The results of Western blot experiments are consistent with the results of immunofluorescence experiments. Our data indicate that Macamide B may exert a neuroprotective effect on HIBD in newborn mice by up-regulating the expression of SIRT1. Macamide B may become a new medicine effective in preventing and treating HIBD.},
 year = {2021}
}

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TY - JOUR
T1 - Effects of Macamide B on the Expression of SIRT1 in Brain Tissue of Neonatal Mice with Hypoxic-ischemic Brain Damage
AU - Ke Niu
AU - Fengjie Xu
AU - Guangyong Wang
AU - Manhong Zeng
Y1 - 2021/07/15
PY - 2021
N1 - https://doi.org/10.11648/j.bs.20210703.11
DO - 10.11648/j.bs.20210703.11
T2 - Biomedical Sciences
JF - Biomedical Sciences
JO - Biomedical Sciences
SP - 64
EP - 68
PB - Science Publishing Group
SN - 2575-3932
UR - https://doi.org/10.11648/j.bs.20210703.11
AB - Neonatal hypoxic-ischemic brain damage (HIBD) is the main cause of severe neurological diseases and death in newborns. Macamide B is an effective monomer extracted from Maca (Lepidium meyenii Walpers), which has important biological activities such as neuroprotection and neuromodulation. The purpose of this study was to investigate whether Macamide B can exert neuroprotective effects on HIBD in newborn mice by regulating Silent information regulator factor 2-related enzyme 1 (SIRT1). A modified Rice-Vannucci method was used to construct the HIBD model of newborn mice. The pups were divided into the following groups: sham group, HI group, and Macamide B group. On the first and third days after hypoxic-ischemic (HI), immunofluorescence and Western blot experiments were used to detect the expression level of SIRT1 in the brain tissue of infants. The results of the immunofluorescence experiment showed that compared with the sham group, the expression level of SIRT1 is significantly decreased in HI group pups, while the expression level of SIRT1 in pups pretreated with Macamide B increased significantly. The results of Western blot experiments are consistent with the results of immunofluorescence experiments. Our data indicate that Macamide B may exert a neuroprotective effect on HIBD in newborn mice by up-regulating the expression of SIRT1. Macamide B may become a new medicine effective in preventing and treating HIBD.
VL - 7
IS - 3
ER -

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Author Information

Ke Niu

School of Clinical Medicine, Guangdong Pharmaceutical University, Guangzhou, People's Republic of China
Fengjie Xu

School of Clinical Medicine, Guangdong Pharmaceutical University, Guangzhou, People's Republic of China
Guangyong Wang

Department of Forensic Science, Intermediate People’s Court of Huangshan City, Huangshan, People's Republic of China
Manhong Zeng

School of Biosciences & Biopharmaceutics, Guangdong Pharmaceutical University, Guangzhou, People's Republic of China

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APA Style

Ke Niu, Fengjie Xu, Guangyong Wang, Manhong Zeng. (2021). Effects of Macamide B on the Expression of SIRT1 in Brain Tissue of Neonatal Mice with Hypoxic-ischemic Brain Damage. Biomedical Sciences, 7(3), 64-68. https://doi.org/10.11648/j.bs.20210703.11

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ACS Style

Ke Niu; Fengjie Xu; Guangyong Wang; Manhong Zeng. Effects of Macamide B on the Expression of SIRT1 in Brain Tissue of Neonatal Mice with Hypoxic-ischemic Brain Damage. Biomed. Sci. 2021, 7(3), 64-68. doi: 10.11648/j.bs.20210703.11

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AMA Style

Ke Niu, Fengjie Xu, Guangyong Wang, Manhong Zeng. Effects of Macamide B on the Expression of SIRT1 in Brain Tissue of Neonatal Mice with Hypoxic-ischemic Brain Damage. Biomed Sci. 2021;7(3):64-68. doi: 10.11648/j.bs.20210703.11

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@article{10.11648/j.bs.20210703.11,
  author = {Ke Niu and Fengjie Xu and Guangyong Wang and Manhong Zeng},
  title = {Effects of Macamide B on the Expression of SIRT1 in Brain Tissue of Neonatal Mice with Hypoxic-ischemic Brain Damage},
  journal = {Biomedical Sciences},
  volume = {7},
  number = {3},
  pages = {64-68},
  doi = {10.11648/j.bs.20210703.11},
  url = {https://doi.org/10.11648/j.bs.20210703.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bs.20210703.11},
  abstract = {Neonatal hypoxic-ischemic brain damage (HIBD) is the main cause of severe neurological diseases and death in newborns. Macamide B is an effective monomer extracted from Maca (Lepidium meyenii Walpers), which has important biological activities such as neuroprotection and neuromodulation. The purpose of this study was to investigate whether Macamide B can exert neuroprotective effects on HIBD in newborn mice by regulating Silent information regulator factor 2-related enzyme 1 (SIRT1). A modified Rice-Vannucci method was used to construct the HIBD model of newborn mice. The pups were divided into the following groups: sham group, HI group, and Macamide B group. On the first and third days after hypoxic-ischemic (HI), immunofluorescence and Western blot experiments were used to detect the expression level of SIRT1 in the brain tissue of infants. The results of the immunofluorescence experiment showed that compared with the sham group, the expression level of SIRT1 is significantly decreased in HI group pups, while the expression level of SIRT1 in pups pretreated with Macamide B increased significantly. The results of Western blot experiments are consistent with the results of immunofluorescence experiments. Our data indicate that Macamide B may exert a neuroprotective effect on HIBD in newborn mice by up-regulating the expression of SIRT1. Macamide B may become a new medicine effective in preventing and treating HIBD.},
 year = {2021}
}

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