Influenza B is one of the main pathogens of human infection, especially among children and adolescents. The incidence rate and mortality rate are higher after infection. Vaccines and drugs are the most effective means to prevent and treat influenza virus. However, due to the characteristics of easy mutation of influenza virus, there are many drug-resistant strains in clinical using. In this study, we analyzed the molecular characterization of neuraminidase (NA) genes and NA drug resistance of influenza B virus in Shangrao during 2019 years. The specimens of nasopharyngeal swabs were collected from influenza cases of monitor hospitals, and then the collection of 10 strains of influenza B virus were randomly selected for detection by MDCK, positive strains were identified by hemagglutination test (HA) and hemagglutination inhibition test (HI). And virus RNA were extracted with QIAamp Viral RNA Mini Kit. Fragments of NA genes were amplified by one-step RT-PCR and then were sequenced. The data obtained were analyzed with the software DNAStar 6.0 and Mage 5.0. We observed that the nucleotides of NA gene of 10 strains had no mutation in catalytic residues and framework residues of NA gene. And strongly suggest that all viruses were sensitive to neuraminidase inhibitors, however continuous resistance surveillance is necessary for control and prevention influenza.
| Published in | Biomedical Sciences (Volume 7, Issue 3) |
| DOI | 10.11648/j.bs.20210703.16 |
| Page(s) | 95-98 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2021. Published by Science Publishing Group |
Influenza B Virus, Resistance, NA Gene
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APA Style
Changhe Cheng, Lanxing Liu, Xiaolong Liu, Zhang Yanyan, Lai Yu. (2021). Analyzed the Resistance of Influenza B Virus in Shangrao. Biomedical Sciences, 7(3), 95-98. https://doi.org/10.11648/j.bs.20210703.16
ACS Style
Changhe Cheng; Lanxing Liu; Xiaolong Liu; Zhang Yanyan; Lai Yu. Analyzed the Resistance of Influenza B Virus in Shangrao. Biomed. Sci. 2021, 7(3), 95-98. doi: 10.11648/j.bs.20210703.16
AMA Style
Changhe Cheng, Lanxing Liu, Xiaolong Liu, Zhang Yanyan, Lai Yu. Analyzed the Resistance of Influenza B Virus in Shangrao. Biomed Sci. 2021;7(3):95-98. doi: 10.11648/j.bs.20210703.16
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Download@article{10.11648/j.bs.20210703.16,
author = {Changhe Cheng and Lanxing Liu and Xiaolong Liu and Zhang Yanyan and Lai Yu},
title = {Analyzed the Resistance of Influenza B Virus in Shangrao},
journal = {Biomedical Sciences},
volume = {7},
number = {3},
pages = {95-98},
doi = {10.11648/j.bs.20210703.16},
url = {https://doi.org/10.11648/j.bs.20210703.16},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bs.20210703.16},
abstract = {Influenza B is one of the main pathogens of human infection, especially among children and adolescents. The incidence rate and mortality rate are higher after infection. Vaccines and drugs are the most effective means to prevent and treat influenza virus. However, due to the characteristics of easy mutation of influenza virus, there are many drug-resistant strains in clinical using. In this study, we analyzed the molecular characterization of neuraminidase (NA) genes and NA drug resistance of influenza B virus in Shangrao during 2019 years. The specimens of nasopharyngeal swabs were collected from influenza cases of monitor hospitals, and then the collection of 10 strains of influenza B virus were randomly selected for detection by MDCK, positive strains were identified by hemagglutination test (HA) and hemagglutination inhibition test (HI). And virus RNA were extracted with QIAamp Viral RNA Mini Kit. Fragments of NA genes were amplified by one-step RT-PCR and then were sequenced. The data obtained were analyzed with the software DNAStar 6.0 and Mage 5.0. We observed that the nucleotides of NA gene of 10 strains had no mutation in catalytic residues and framework residues of NA gene. And strongly suggest that all viruses were sensitive to neuraminidase inhibitors, however continuous resistance surveillance is necessary for control and prevention influenza.},
year = {2021}
}
TY - JOUR T1 - Analyzed the Resistance of Influenza B Virus in Shangrao AU - Changhe Cheng AU - Lanxing Liu AU - Xiaolong Liu AU - Zhang Yanyan AU - Lai Yu Y1 - 2021/09/30 PY - 2021 N1 - https://doi.org/10.11648/j.bs.20210703.16 DO - 10.11648/j.bs.20210703.16 T2 - Biomedical Sciences JF - Biomedical Sciences JO - Biomedical Sciences SP - 95 EP - 98 PB - Science Publishing Group SN - 2575-3932 UR - https://doi.org/10.11648/j.bs.20210703.16 AB - Influenza B is one of the main pathogens of human infection, especially among children and adolescents. The incidence rate and mortality rate are higher after infection. Vaccines and drugs are the most effective means to prevent and treat influenza virus. However, due to the characteristics of easy mutation of influenza virus, there are many drug-resistant strains in clinical using. In this study, we analyzed the molecular characterization of neuraminidase (NA) genes and NA drug resistance of influenza B virus in Shangrao during 2019 years. The specimens of nasopharyngeal swabs were collected from influenza cases of monitor hospitals, and then the collection of 10 strains of influenza B virus were randomly selected for detection by MDCK, positive strains were identified by hemagglutination test (HA) and hemagglutination inhibition test (HI). And virus RNA were extracted with QIAamp Viral RNA Mini Kit. Fragments of NA genes were amplified by one-step RT-PCR and then were sequenced. The data obtained were analyzed with the software DNAStar 6.0 and Mage 5.0. We observed that the nucleotides of NA gene of 10 strains had no mutation in catalytic residues and framework residues of NA gene. And strongly suggest that all viruses were sensitive to neuraminidase inhibitors, however continuous resistance surveillance is necessary for control and prevention influenza. VL - 7 IS - 3 ER -
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