Screening for Hydrocarbon Degrading Bacteria Using Redox Indicator 2, 6-Dichlorophenol Indophenol

James Iniobong Ime; Ibuot Aniefon Alphonsus; Akpan Patience Saturday; Ben Mayen Godwin; Etuk Christiana Utibe; Umoren Emmanuel Anthony

doi:doi:10.11648/j.cbe.20180302.11

Screening for Hydrocarbon Degrading Bacteria Using Redox Indicator 2, 6-Dichlorophenol Indophenol

James Iniobong Ime, Ibuot Aniefon Alphonsus, Akpan Patience Saturday, Ben Mayen Godwin, Etuk Christiana Utibe, Umoren Emmanuel Anthony

Published in Chemical and Biomolecular Engineering (Volume 3, Issue 2)

Received: 2 August 2018 Accepted: 7 September 2018 Published: 13 October 2018

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Abstract

Contamination by petroleum products and its derivatives promotes serious environmental damage. Biodegradation capacity studies are important when deciding the correct bioremediation strategy to employ. The use of redox indicator 2, 6-Dichlorophenol Indophenol (DCPIP) is a rapid, simple and low cost model for evaluating capability of microorganisms to utilize and/or degrade petroleum hydrocarbons. This study involved isolation and screening of bacterial species capable of utilizing hydrocarbons from soil at two auto-mechanic workshops in Uyo, Akwa Ibom State. Results of the physicochemical analysis of the soil samples showed higher levels of properties (Moisture content, Organic Carbon content, Total Hydrocarbon Content) in the polluted soil samples when compared with unpolluted (control) soil sample. Total heterotrophic bacterial populations in polluted soil samples ranged between 4.4±1.90x10⁷ and 6.0±32.0x10⁷ CFU/g while hydrocarbon utilizing bacterial counts were between 3.2±0.05x10⁷ and 5.2±25.2x10⁷ CFU/g. Eight bacteria species capable of utilizing petroleum were isolated from these soils by enrichment technique. Isolated bacteria include: Corynebacterium spp, Pseudomonas aeruginosa, Micrococcus agilis, Flavobacterium aquatile, Staphylococcus aureus, Micrococcus luteus, Serratia odorifera and Bacillus substilis. Screening of bacterial isolates for efficiency in hydrocarbon utilization using DCPIP, measured by using colorimetry, revealed Bacillus substilis, Pseudomonas aeruginosa, Micrococccus agilis, Flavobacterium aquatile Micrococcus luteus and Corynebacterium spp to be able to rapidly utilize hydrocarbons from waste engine oil.

Published in	Chemical and Biomolecular Engineering (Volume 3, Issue 2)
DOI	10.11648/j.cbe.20180302.11
Page(s)	11-16
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2018. Published by Science Publishing Group

Keywords

Hydrocarbon, Bacteria, 2, 6-Dichlorophenol Indophenols

References

[1]	Atlas, R. M. (1995). Bioremediation of petroleum pollutants. International Biodeterioration and Biodegradation, 35 (1-3):317-327
[2]	Kadafa, A. A. (2012). Oil Exploration and Spillage in the Niger Delta of Nigeria. Civil and Environmental Research, 2 (3):38-51.
[3]	Atlas, R. M. (1981). Microbial degradation of petroleum hydrocarbons: an environmental perspective. Microbiological Reviews, 45 (1):180–209.
[4]	Bartha, R., and Atlas. R. M. (1977). The microbiology of aquatic oil spills. Advances in Applied Microbiology, 22:225-266.
[5]	Makut, M. D. and Ishaya, P. (2010). Bacterial species associated with soils contaminated with used petroleum products in Keffi town, Nigeria. African Journal of Microbiology Research, 4 (16):1698-1702.
[6]	Bamforth, S. M. and Singleton, I. (2005). Bioremediation of polycyclic aromatic hydrocarbons: current knowledge and future directions. Journal of Chemical Technology and Biotechnology, 80:723–736.
[7]	Vidali, M. (2001). Bioremediation: an overview. Pure and Applied Chemistry, 73: 1163-1172.
[8]	Balba, M. T., Al-Awadhi, N. and Al-Daher, R. (1998). Bioremediation of oil- contaminated soil: microbiological methods for feasibility assessment and field evaluation. Journal of Microbiological Methods, 32:155–164.
[9]	Malatova, K. (2005). A thesis submitted in partial fulfillment of the requirement for the Degree of Master of Science in Chemistry. Department of Chemistry, Rochester Institute of Technology, Rochester, NY.
[10]	Okpokwasili, G. C. and Amanchukwu, S. C. (1989). Hydrocarbon degradation and utilization by palm wine isolates. FEMS Microbiology Letters, 57:151-154.
[11]	Desai, A. and Vyas P. (2006). Petroleum and hydrocarbon microbiology, Department of Microbiology, M. S. University of Baroda, Vadodara, Pp, 1-22. http://nsdl.niscair.res.in/jspui/bitstream .
[12]	Vernon, L. P. and Zaugg, W. S. (1960). Photoreductions by fresh and aged chloroplasts; requirement for ascorbate and 2, 6-dichlorophenolindophenol with aged chloroplasts. Journal of Biological Chemistry, 235:2728-2733.
[13]	Bidoia E. D., Montagnolli R. N. and Lopes P. R. M. (2010), Microbial biodegradation potential of hydrocarbons evaluated by colorimetric technique: a case study, In: Current Research, Technology and Education Topics in Applied Microbiology and Microbial Biotechnology, Mendez-Vilas A. (Ed.), FORMATEX, Badajoz, Spain.
[14]	Hanson, K. G., Desai, J. D. and Desai, A. J. (1993). A rapid and simple screening technique for potential crude oil degrading microorganisms. Biotechnology Techniques, 7:745–748.
[15]	Mariano, A. P., Bonotto, D. M., Angelis, D. F., Pirollo, M. P. S. and Contiero, J. (2008), Biodegradability of commercial and weathered diesel oils. Brazilian Journal of Microbiology, 39:33-142.
[16]	Patil, T. D., Pawar, S., Kamble, P. N. and Thakare, S. V. (2012), Bioremediation of complex hydrocarbons using microbial consortium isolated from diesel oil polluted soil. Der Chemica Sinica, 3:953-958.
[17]	Balogun, S. A., Shofola, T. C., Okedeji, A. O. and Ayangbenro, A. S. (2015). Screening of hydrocarbonoclastic bacteria using redox indicator 2, 6-dichlorophenol indophenol. Global Nest Jour., 17: 1-9.
[18]	Cheesbrough, M. (2006). District Laboratory Practice in Tropical Countries (Part 2) Cambridge University Press. Hon Kong. Pp 64-70.
[19]	Holt, J. G., Krieg, N. R., Sneath, P. H. A., Stanley, J. T., William, S. T. (1994). Bergey's Manual of Determinative Bacteriology. 9th edition. William and Wilkins, Baltimore.
[20]	AOAC (2003). Official methods of analysis of the Association of Official analytical chemist, 17th Edition. Association of Official analytical chemist, Arlington, Virginia. Pp. 96-105.
[21]	Chikere, C. B. (2012). Culture-Independent analysis of bacterial community composition during bioremediation of crude oil-polluted soil. British Microbiology Resources Journal, 2 (3):187-211.
[22]	Chikere, C. B. and Ekwuabu, C. B. (2014). Culture-independent characterization of hydrocarbon utilizing bacteria in selected crude oil-impacted sites in Bodo, Ogoniland. African Journal of Environmental Science and Technology, 8 (6): 401-406.
[23]	Chikere, C. B. (2010). Bacterial diversity and community dynamics during the bioremediation of crude oil-polluted soil. Dissertation. Microbiology Department. University of Port Harcourt.
[24]	Eweis, J. B., Ergas, S. J., Chang, D. P. Y. and Schroeder, E. D. (1998). Bioremediation principles. McGraw-Hill Companies. Inc., New York. Pp. 296.
[25]	Aparna, C., Saitha, P., Himabindu, V., Alok, B. and Anjaneyulu, Y. (2010). Evaluation of bioremediation effectiveness on sediments contaminated with industrial wastes. International Journal Environmental Science, 1 (4): 607-620.
[26]	Eze, V. C. and Okpokwasili, G. C. (2010). Microbial and other related changes in Niger Delta River sediment receiving industrial effluents. Continental Journal of Microbiology, 4:15-24.
[27]	Ogunbayo, A. O. Bello, R. A. and Nwagbara, U. (2012). Bioremediation of engine oil contaminated site. Journal of Emerging Trends in Engineering and Applied Sciences, 3 (3): 483-489.
[28]	Ibiene, A. A., Orji, F. A. and Orji-Nwosu, E. C. (2011). Microbial populationdynamics in crude oil-polluted soils in the Niger Delta. Nigerian Journal of Agriculture Food Environmental, 7:8-13.
[29]	Obire, O. and Nwaubeta, O. (2002). Biodegradation of refined petroleum hydrocarbons in soil. JASEM. 5:43-46.
[30]	Sarma, A. and Sarma, H. (2010). Enhanced biodegradation by some microbial isolates supplemented with heavy metals. International Journal of Botany, 6:441-448.
[31]	Ebrahimi, M., Sarikkhani, M. R. and Fallah. R. (2012). Assessment of biodegradation efficiency of some isolated bacteria from oil-contaminated sites in solid and liquid media containing oil-compounds. International Resources Journal of Applied and Basic Science, 3 (1):138-147.
[32]	Selvakumar, S., Sekar, P., Rajakumar, S. and Ayyasamy, P. M. (2014). Rapid screening of crude oil degrading bacteria isolated from oil contaminated areas. The Scitech Journal, 1:24-27.
[33]	Atlas R. M. (1992). Petroleum Microbiology. Academic Press, Baltimore, USA.
[34]	Okoh, A. I. and Trejo-Hernandez, M. R. (2006). Remediation of petroleum hydrocarbon polluted systems: exploiting the bioremediation strategies. African Journal of Biotechnology, 5: 2520-2525.
[35]	Yoshida, N., Hoashi, J., Morita, T., McNiven, S. J., Nakamura, H. and Karube, I. (2001), Improvement of a mediator-type biochemical oxygen demand sensor for on-site measurement. Journal of Biotechnology, 88: 269-275.
[36]	Banat J. M., Makkar, R. S. and Cameotra, S. S. (2000), Potential commercial applications of microbial surfactants. Applied Microbiology and Biotechnology, 53: 495-508.
[37]	Saadoun, I. (2002). Isolation and characterization of bacteria from crude petroleum oil contaminated soil and their potential to degrade diesel fuel. Journal of Basic Microbiology, 42:420-428.

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James Iniobong Ime, Ibuot Aniefon Alphonsus, Akpan Patience Saturday, Ben Mayen Godwin, Etuk Christiana Utibe, et al. (2018). Screening for Hydrocarbon Degrading Bacteria Using Redox Indicator 2, 6-Dichlorophenol Indophenol. Chemical and Biomolecular Engineering, 3(2), 11-16. https://doi.org/10.11648/j.cbe.20180302.11

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ACS Style

James Iniobong Ime; Ibuot Aniefon Alphonsus; Akpan Patience Saturday; Ben Mayen Godwin; Etuk Christiana Utibe, et al. Screening for Hydrocarbon Degrading Bacteria Using Redox Indicator 2, 6-Dichlorophenol Indophenol. Chem. Biomol. Eng. 2018, 3(2), 11-16. doi: 10.11648/j.cbe.20180302.11

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AMA Style

James Iniobong Ime, Ibuot Aniefon Alphonsus, Akpan Patience Saturday, Ben Mayen Godwin, Etuk Christiana Utibe, et al. Screening for Hydrocarbon Degrading Bacteria Using Redox Indicator 2, 6-Dichlorophenol Indophenol. Chem Biomol Eng. 2018;3(2):11-16. doi: 10.11648/j.cbe.20180302.11

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@article{10.11648/j.cbe.20180302.11,
  author = {James Iniobong Ime and Ibuot Aniefon Alphonsus and Akpan Patience Saturday and Ben Mayen Godwin and Etuk Christiana Utibe and Umoren Emmanuel Anthony},
  title = {Screening for Hydrocarbon Degrading Bacteria Using Redox Indicator 2, 6-Dichlorophenol Indophenol},
  journal = {Chemical and Biomolecular Engineering},
  volume = {3},
  number = {2},
  pages = {11-16},
  doi = {10.11648/j.cbe.20180302.11},
  url = {https://doi.org/10.11648/j.cbe.20180302.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.cbe.20180302.11},
  abstract = {Contamination by petroleum products and its derivatives promotes serious environmental damage. Biodegradation capacity studies are important when deciding the correct bioremediation strategy to employ. The use of redox indicator 2, 6-Dichlorophenol Indophenol (DCPIP) is a rapid, simple and low cost model for evaluating capability of microorganisms to utilize and/or degrade petroleum hydrocarbons. This study involved isolation and screening of bacterial species capable of utilizing hydrocarbons from soil at two auto-mechanic workshops in Uyo, Akwa Ibom State. Results of the physicochemical analysis of the soil samples showed higher levels of properties (Moisture content, Organic Carbon content, Total Hydrocarbon Content) in the polluted soil samples when compared with unpolluted (control) soil sample. Total heterotrophic bacterial populations in polluted soil samples ranged between 4.4±1.90x107 and 6.0±32.0x107 CFU/g while hydrocarbon utilizing bacterial counts were between 3.2±0.05x107 and 5.2±25.2x107 CFU/g. Eight bacteria species capable of utilizing petroleum were isolated from these soils by enrichment technique. Isolated bacteria include: Corynebacterium spp, Pseudomonas aeruginosa, Micrococcus agilis, Flavobacterium aquatile, Staphylococcus aureus, Micrococcus luteus, Serratia odorifera and Bacillus substilis. Screening of bacterial isolates for efficiency in hydrocarbon utilization using DCPIP, measured by using colorimetry, revealed Bacillus substilis, Pseudomonas aeruginosa, Micrococccus agilis, Flavobacterium aquatile Micrococcus luteus and Corynebacterium spp to be able to rapidly utilize hydrocarbons from waste engine oil.},
 year = {2018}
}

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TY  - JOUR
T1  - Screening for Hydrocarbon Degrading Bacteria Using Redox Indicator 2, 6-Dichlorophenol Indophenol
AU  - James Iniobong Ime
AU  - Ibuot Aniefon Alphonsus
AU  - Akpan Patience Saturday
AU  - Ben Mayen Godwin
AU  - Etuk Christiana Utibe
AU  - Umoren Emmanuel Anthony
Y1  - 2018/10/13
PY  - 2018
N1  - https://doi.org/10.11648/j.cbe.20180302.11
DO  - 10.11648/j.cbe.20180302.11
T2  - Chemical and Biomolecular Engineering
JF  - Chemical and Biomolecular Engineering
JO  - Chemical and Biomolecular Engineering
SP  - 11
EP  - 16
PB  - Science Publishing Group
SN  - 2578-8884
UR  - https://doi.org/10.11648/j.cbe.20180302.11
AB  - Contamination by petroleum products and its derivatives promotes serious environmental damage. Biodegradation capacity studies are important when deciding the correct bioremediation strategy to employ. The use of redox indicator 2, 6-Dichlorophenol Indophenol (DCPIP) is a rapid, simple and low cost model for evaluating capability of microorganisms to utilize and/or degrade petroleum hydrocarbons. This study involved isolation and screening of bacterial species capable of utilizing hydrocarbons from soil at two auto-mechanic workshops in Uyo, Akwa Ibom State. Results of the physicochemical analysis of the soil samples showed higher levels of properties (Moisture content, Organic Carbon content, Total Hydrocarbon Content) in the polluted soil samples when compared with unpolluted (control) soil sample. Total heterotrophic bacterial populations in polluted soil samples ranged between 4.4±1.90x107 and 6.0±32.0x107 CFU/g while hydrocarbon utilizing bacterial counts were between 3.2±0.05x107 and 5.2±25.2x107 CFU/g. Eight bacteria species capable of utilizing petroleum were isolated from these soils by enrichment technique. Isolated bacteria include: Corynebacterium spp, Pseudomonas aeruginosa, Micrococcus agilis, Flavobacterium aquatile, Staphylococcus aureus, Micrococcus luteus, Serratia odorifera and Bacillus substilis. Screening of bacterial isolates for efficiency in hydrocarbon utilization using DCPIP, measured by using colorimetry, revealed Bacillus substilis, Pseudomonas aeruginosa, Micrococccus agilis, Flavobacterium aquatile Micrococcus luteus and Corynebacterium spp to be able to rapidly utilize hydrocarbons from waste engine oil.
VL  - 3
IS  - 2
ER  -

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Author Information

James Iniobong Ime

Department of Science Technology, Akwa Ibom State Polytechnic, Ikot Osurua, Ikot Ekpene, Nigeria
Ibuot Aniefon Alphonsus

Department of Science Technology, Akwa Ibom State Polytechnic, Ikot Osurua, Ikot Ekpene, Nigeria
Akpan Patience Saturday

Department of Science Technology, Akwa Ibom State Polytechnic, Ikot Osurua, Ikot Ekpene, Nigeria
Ben Mayen Godwin

Department of Science Technology, Akwa Ibom State Polytechnic, Ikot Osurua, Ikot Ekpene, Nigeria
Etuk Christiana Utibe

Department of Science Technology, Akwa Ibom State Polytechnic, Ikot Osurua, Ikot Ekpene, Nigeria
Umoren Emmanuel Anthony

Department of Science Technology, Akwa Ibom State Polytechnic, Ikot Osurua, Ikot Ekpene, Nigeria

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APA Style

James Iniobong Ime, Ibuot Aniefon Alphonsus, Akpan Patience Saturday, Ben Mayen Godwin, Etuk Christiana Utibe, et al. (2018). Screening for Hydrocarbon Degrading Bacteria Using Redox Indicator 2, 6-Dichlorophenol Indophenol. Chemical and Biomolecular Engineering, 3(2), 11-16. https://doi.org/10.11648/j.cbe.20180302.11

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ACS Style

James Iniobong Ime; Ibuot Aniefon Alphonsus; Akpan Patience Saturday; Ben Mayen Godwin; Etuk Christiana Utibe, et al. Screening for Hydrocarbon Degrading Bacteria Using Redox Indicator 2, 6-Dichlorophenol Indophenol. Chem. Biomol. Eng. 2018, 3(2), 11-16. doi: 10.11648/j.cbe.20180302.11

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AMA Style

James Iniobong Ime, Ibuot Aniefon Alphonsus, Akpan Patience Saturday, Ben Mayen Godwin, Etuk Christiana Utibe, et al. Screening for Hydrocarbon Degrading Bacteria Using Redox Indicator 2, 6-Dichlorophenol Indophenol. Chem Biomol Eng. 2018;3(2):11-16. doi: 10.11648/j.cbe.20180302.11

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@article{10.11648/j.cbe.20180302.11,
  author = {James Iniobong Ime and Ibuot Aniefon Alphonsus and Akpan Patience Saturday and Ben Mayen Godwin and Etuk Christiana Utibe and Umoren Emmanuel Anthony},
  title = {Screening for Hydrocarbon Degrading Bacteria Using Redox Indicator 2, 6-Dichlorophenol Indophenol},
  journal = {Chemical and Biomolecular Engineering},
  volume = {3},
  number = {2},
  pages = {11-16},
  doi = {10.11648/j.cbe.20180302.11},
  url = {https://doi.org/10.11648/j.cbe.20180302.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.cbe.20180302.11},
  abstract = {Contamination by petroleum products and its derivatives promotes serious environmental damage. Biodegradation capacity studies are important when deciding the correct bioremediation strategy to employ. The use of redox indicator 2, 6-Dichlorophenol Indophenol (DCPIP) is a rapid, simple and low cost model for evaluating capability of microorganisms to utilize and/or degrade petroleum hydrocarbons. This study involved isolation and screening of bacterial species capable of utilizing hydrocarbons from soil at two auto-mechanic workshops in Uyo, Akwa Ibom State. Results of the physicochemical analysis of the soil samples showed higher levels of properties (Moisture content, Organic Carbon content, Total Hydrocarbon Content) in the polluted soil samples when compared with unpolluted (control) soil sample. Total heterotrophic bacterial populations in polluted soil samples ranged between 4.4±1.90x107 and 6.0±32.0x107 CFU/g while hydrocarbon utilizing bacterial counts were between 3.2±0.05x107 and 5.2±25.2x107 CFU/g. Eight bacteria species capable of utilizing petroleum were isolated from these soils by enrichment technique. Isolated bacteria include: Corynebacterium spp, Pseudomonas aeruginosa, Micrococcus agilis, Flavobacterium aquatile, Staphylococcus aureus, Micrococcus luteus, Serratia odorifera and Bacillus substilis. Screening of bacterial isolates for efficiency in hydrocarbon utilization using DCPIP, measured by using colorimetry, revealed Bacillus substilis, Pseudomonas aeruginosa, Micrococccus agilis, Flavobacterium aquatile Micrococcus luteus and Corynebacterium spp to be able to rapidly utilize hydrocarbons from waste engine oil.},
 year = {2018}
}

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TY  - JOUR
T1  - Screening for Hydrocarbon Degrading Bacteria Using Redox Indicator 2, 6-Dichlorophenol Indophenol
AU  - James Iniobong Ime
AU  - Ibuot Aniefon Alphonsus
AU  - Akpan Patience Saturday
AU  - Ben Mayen Godwin
AU  - Etuk Christiana Utibe
AU  - Umoren Emmanuel Anthony
Y1  - 2018/10/13
PY  - 2018
N1  - https://doi.org/10.11648/j.cbe.20180302.11
DO  - 10.11648/j.cbe.20180302.11
T2  - Chemical and Biomolecular Engineering
JF  - Chemical and Biomolecular Engineering
JO  - Chemical and Biomolecular Engineering
SP  - 11
EP  - 16
PB  - Science Publishing Group
SN  - 2578-8884
UR  - https://doi.org/10.11648/j.cbe.20180302.11
AB  - Contamination by petroleum products and its derivatives promotes serious environmental damage. Biodegradation capacity studies are important when deciding the correct bioremediation strategy to employ. The use of redox indicator 2, 6-Dichlorophenol Indophenol (DCPIP) is a rapid, simple and low cost model for evaluating capability of microorganisms to utilize and/or degrade petroleum hydrocarbons. This study involved isolation and screening of bacterial species capable of utilizing hydrocarbons from soil at two auto-mechanic workshops in Uyo, Akwa Ibom State. Results of the physicochemical analysis of the soil samples showed higher levels of properties (Moisture content, Organic Carbon content, Total Hydrocarbon Content) in the polluted soil samples when compared with unpolluted (control) soil sample. Total heterotrophic bacterial populations in polluted soil samples ranged between 4.4±1.90x107 and 6.0±32.0x107 CFU/g while hydrocarbon utilizing bacterial counts were between 3.2±0.05x107 and 5.2±25.2x107 CFU/g. Eight bacteria species capable of utilizing petroleum were isolated from these soils by enrichment technique. Isolated bacteria include: Corynebacterium spp, Pseudomonas aeruginosa, Micrococcus agilis, Flavobacterium aquatile, Staphylococcus aureus, Micrococcus luteus, Serratia odorifera and Bacillus substilis. Screening of bacterial isolates for efficiency in hydrocarbon utilization using DCPIP, measured by using colorimetry, revealed Bacillus substilis, Pseudomonas aeruginosa, Micrococccus agilis, Flavobacterium aquatile Micrococcus luteus and Corynebacterium spp to be able to rapidly utilize hydrocarbons from waste engine oil.
VL  - 3
IS  - 2
ER  -

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