Abelmoschus esculentus is an important medicinal plant belongs to family Malvaceae. It originates from Ethiopia and is widely spread all over tropical, subtropical and warm temperate regions of the world. This research work has been designed to evaluate the antioxidant, antimicrobial and toxicological potential of A. esculentus leaves and seeds. The antifungal and antioxidant components of A. esculentus leaves and seeds were extracted by using four solvent systems (80% methanol, 80% ethanol, 100% methanol and 100% ethanol) and leaves presented maximum extract yield (38.1 g/100g DW)in 80% methanolic solvent system. Phytochemical analysis of A. esculentus leaves and seeds extracts performed in terms of total phenolic and total flavonoid contents, showed that 80% methanolic leaves extract offered highest total phenolic contents (31.2 mg GAE/g DW), whereas 80% ethanolic leaves gave maximum total flavonoid contents (41.8 mg CE/g DW). Antioxidant activity was determined by DPPH radical scavenging activity and measure of reducing power. Results revealed that 80% methanolic leaves extract showed highest radical scavenging activity and reducing potential. Antimicrobial activity of A. esculentus leaves and seeds was investigated by Disc Diffusion Method and Minimum Inhibitory Concentration (MIC). Results showed that 80% methanolic extract of leaves exhibited highest antibacterial and antifungal potential against P. multocida (30 mm DIZ) and A. paraciticus (29 mm DIZ), respectively. Cytotoxicity analysis was performed on BHK-21 cell by adopting the MTT assay. The cytotoxicity activity of the 80% methanolic extract of leaves was evaluated by noticing the cell survival percentage (52.5%). Overall results of the present study showed that 80% methanolic leaves extracts of A. esculentus possesses very good antioxidant, antimicrobial and cytotoxic properties.
| Published in | Chemical and Biomolecular Engineering (Volume 5, Issue 4) |
| DOI | 10.11648/j.cbe.20200504.11 |
| Page(s) | 69-79 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2020. Published by Science Publishing Group |
Antioxidant, Antibacterial, Scavenging, Cytotoxicity, Potential
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APA Style
Mamuna Hafeez, Syeda Mona Hassan, Shahzad Sharif Mughal, Muneeza Munir, Muhammad Kamran Khan. (2020). Antioxidant, Antimicrobial and Cytotoxic Potential of Abelmoschus esculentus. Chemical and Biomolecular Engineering, 5(4), 69-79. https://doi.org/10.11648/j.cbe.20200504.11
ACS Style
Mamuna Hafeez; Syeda Mona Hassan; Shahzad Sharif Mughal; Muneeza Munir; Muhammad Kamran Khan. Antioxidant, Antimicrobial and Cytotoxic Potential of Abelmoschus esculentus. Chem. Biomol. Eng. 2020, 5(4), 69-79. doi: 10.11648/j.cbe.20200504.11
AMA Style
Mamuna Hafeez, Syeda Mona Hassan, Shahzad Sharif Mughal, Muneeza Munir, Muhammad Kamran Khan. Antioxidant, Antimicrobial and Cytotoxic Potential of Abelmoschus esculentus. Chem Biomol Eng. 2020;5(4):69-79. doi: 10.11648/j.cbe.20200504.11
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Download@article{10.11648/j.cbe.20200504.11,
author = {Mamuna Hafeez and Syeda Mona Hassan and Shahzad Sharif Mughal and Muneeza Munir and Muhammad Kamran Khan},
title = {Antioxidant, Antimicrobial and Cytotoxic Potential of Abelmoschus esculentus},
journal = {Chemical and Biomolecular Engineering},
volume = {5},
number = {4},
pages = {69-79},
doi = {10.11648/j.cbe.20200504.11},
url = {https://doi.org/10.11648/j.cbe.20200504.11},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.cbe.20200504.11},
abstract = {Abelmoschus esculentus is an important medicinal plant belongs to family Malvaceae. It originates from Ethiopia and is widely spread all over tropical, subtropical and warm temperate regions of the world. This research work has been designed to evaluate the antioxidant, antimicrobial and toxicological potential of A. esculentus leaves and seeds. The antifungal and antioxidant components of A. esculentus leaves and seeds were extracted by using four solvent systems (80% methanol, 80% ethanol, 100% methanol and 100% ethanol) and leaves presented maximum extract yield (38.1 g/100g DW)in 80% methanolic solvent system. Phytochemical analysis of A. esculentus leaves and seeds extracts performed in terms of total phenolic and total flavonoid contents, showed that 80% methanolic leaves extract offered highest total phenolic contents (31.2 mg GAE/g DW), whereas 80% ethanolic leaves gave maximum total flavonoid contents (41.8 mg CE/g DW). Antioxidant activity was determined by DPPH radical scavenging activity and measure of reducing power. Results revealed that 80% methanolic leaves extract showed highest radical scavenging activity and reducing potential. Antimicrobial activity of A. esculentus leaves and seeds was investigated by Disc Diffusion Method and Minimum Inhibitory Concentration (MIC). Results showed that 80% methanolic extract of leaves exhibited highest antibacterial and antifungal potential against P. multocida (30 mm DIZ) and A. paraciticus (29 mm DIZ), respectively. Cytotoxicity analysis was performed on BHK-21 cell by adopting the MTT assay. The cytotoxicity activity of the 80% methanolic extract of leaves was evaluated by noticing the cell survival percentage (52.5%). Overall results of the present study showed that 80% methanolic leaves extracts of A. esculentus possesses very good antioxidant, antimicrobial and cytotoxic properties.},
year = {2020}
}
TY - JOUR T1 - Antioxidant, Antimicrobial and Cytotoxic Potential of Abelmoschus esculentus AU - Mamuna Hafeez AU - Syeda Mona Hassan AU - Shahzad Sharif Mughal AU - Muneeza Munir AU - Muhammad Kamran Khan Y1 - 2020/11/19 PY - 2020 N1 - https://doi.org/10.11648/j.cbe.20200504.11 DO - 10.11648/j.cbe.20200504.11 T2 - Chemical and Biomolecular Engineering JF - Chemical and Biomolecular Engineering JO - Chemical and Biomolecular Engineering SP - 69 EP - 79 PB - Science Publishing Group SN - 2578-8884 UR - https://doi.org/10.11648/j.cbe.20200504.11 AB - Abelmoschus esculentus is an important medicinal plant belongs to family Malvaceae. It originates from Ethiopia and is widely spread all over tropical, subtropical and warm temperate regions of the world. This research work has been designed to evaluate the antioxidant, antimicrobial and toxicological potential of A. esculentus leaves and seeds. The antifungal and antioxidant components of A. esculentus leaves and seeds were extracted by using four solvent systems (80% methanol, 80% ethanol, 100% methanol and 100% ethanol) and leaves presented maximum extract yield (38.1 g/100g DW)in 80% methanolic solvent system. Phytochemical analysis of A. esculentus leaves and seeds extracts performed in terms of total phenolic and total flavonoid contents, showed that 80% methanolic leaves extract offered highest total phenolic contents (31.2 mg GAE/g DW), whereas 80% ethanolic leaves gave maximum total flavonoid contents (41.8 mg CE/g DW). Antioxidant activity was determined by DPPH radical scavenging activity and measure of reducing power. Results revealed that 80% methanolic leaves extract showed highest radical scavenging activity and reducing potential. Antimicrobial activity of A. esculentus leaves and seeds was investigated by Disc Diffusion Method and Minimum Inhibitory Concentration (MIC). Results showed that 80% methanolic extract of leaves exhibited highest antibacterial and antifungal potential against P. multocida (30 mm DIZ) and A. paraciticus (29 mm DIZ), respectively. Cytotoxicity analysis was performed on BHK-21 cell by adopting the MTT assay. The cytotoxicity activity of the 80% methanolic extract of leaves was evaluated by noticing the cell survival percentage (52.5%). Overall results of the present study showed that 80% methanolic leaves extracts of A. esculentus possesses very good antioxidant, antimicrobial and cytotoxic properties. VL - 5 IS - 4 ER -
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