M. charantia is an important medicinal plant belongs to family cucurbitaceae. It originates from India, Malasiya and is widely spread all over tropical, subtropical and warm temperate regions of the world. This research work has been designed to evaluate the antioxidant, antimicrobial and toxicological potential of M. charantia. The antifungal and antioxidant components of M. charantia leaves, seeds and peels were extracted by using four solvent systems (80% methanol, 80% ethanol, 100% methanol and 100% ethanol) andleaves presented maximum extract yield (22.7 g/100g DW) in 80% methanolic solvent system. Phytochemical analysis of M. Charantia leaves, seeds and peels extracts performed in terms of total phenolic and total flavonoid contents, showed that 80% methanolic leaves extract offered highest total phenolic contents (47.1 mg GAE/g DW), whereas80% ethanolic leaves gave maximum total flavonoid contents (67.3 mg CE/g DW). The phenolic contents were also analysed by HPLC. Antioxidant activity was determined by DPPH radical scavenging activity and measure of reducing power. Results revealed that 80% methanolic leaves extract showed highest radical scavenging activity and reducing potential. Antimicrobial activity of M. charantia leaves, seeds and peels was investigated by Disc Diffusion Method and Minimum Inhibitory Concentration (MIC). Results showed that 80% methanolic extract of leaves exhibited highest antibacterial and antifungal potential against P. multocida (30 mm DIZ) and A. paraciticus (28 mm DIZ), respectively. Cytotoxicity analysis was performed on BHK-21 cell by adopting the MTT assay. The cytotoxicity activity of the 80% methanolic extract of leaves was evaluated by noticing the cell survival percentage (53.4%). Overall results of the present study showed that 80% methanolic leaves extracts of M. charantia possesses very good antioxidant, antimicrobial and cytotoxic properties.
| Published in | Chemical and Biomolecular Engineering (Volume 6, Issue 1) |
| DOI | 10.11648/j.cbe.20210601.13 |
| Page(s) | 17-29 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2021. Published by Science Publishing Group |
Momordica charantia, General Description, Medicinal Properties
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APA Style
Zermina Khalid, Syeda Mona Hassan, Shahzad Sharif Mughal, Syed Khurram Hassan, Huma Hassan. (2021). Phenolic Profile and Biological Properties of Momordica charantia. Chemical and Biomolecular Engineering, 6(1), 17-29. https://doi.org/10.11648/j.cbe.20210601.13
ACS Style
Zermina Khalid; Syeda Mona Hassan; Shahzad Sharif Mughal; Syed Khurram Hassan; Huma Hassan. Phenolic Profile and Biological Properties of Momordica charantia. Chem. Biomol. Eng. 2021, 6(1), 17-29. doi: 10.11648/j.cbe.20210601.13
AMA Style
Zermina Khalid, Syeda Mona Hassan, Shahzad Sharif Mughal, Syed Khurram Hassan, Huma Hassan. Phenolic Profile and Biological Properties of Momordica charantia. Chem Biomol Eng. 2021;6(1):17-29. doi: 10.11648/j.cbe.20210601.13
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Download@article{10.11648/j.cbe.20210601.13,
author = {Zermina Khalid and Syeda Mona Hassan and Shahzad Sharif Mughal and Syed Khurram Hassan and Huma Hassan},
title = {Phenolic Profile and Biological Properties of Momordica charantia},
journal = {Chemical and Biomolecular Engineering},
volume = {6},
number = {1},
pages = {17-29},
doi = {10.11648/j.cbe.20210601.13},
url = {https://doi.org/10.11648/j.cbe.20210601.13},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.cbe.20210601.13},
abstract = {M. charantia is an important medicinal plant belongs to family cucurbitaceae. It originates from India, Malasiya and is widely spread all over tropical, subtropical and warm temperate regions of the world. This research work has been designed to evaluate the antioxidant, antimicrobial and toxicological potential of M. charantia. The antifungal and antioxidant components of M. charantia leaves, seeds and peels were extracted by using four solvent systems (80% methanol, 80% ethanol, 100% methanol and 100% ethanol) andleaves presented maximum extract yield (22.7 g/100g DW) in 80% methanolic solvent system. Phytochemical analysis of M. Charantia leaves, seeds and peels extracts performed in terms of total phenolic and total flavonoid contents, showed that 80% methanolic leaves extract offered highest total phenolic contents (47.1 mg GAE/g DW), whereas80% ethanolic leaves gave maximum total flavonoid contents (67.3 mg CE/g DW). The phenolic contents were also analysed by HPLC. Antioxidant activity was determined by DPPH radical scavenging activity and measure of reducing power. Results revealed that 80% methanolic leaves extract showed highest radical scavenging activity and reducing potential. Antimicrobial activity of M. charantia leaves, seeds and peels was investigated by Disc Diffusion Method and Minimum Inhibitory Concentration (MIC). Results showed that 80% methanolic extract of leaves exhibited highest antibacterial and antifungal potential against P. multocida (30 mm DIZ) and A. paraciticus (28 mm DIZ), respectively. Cytotoxicity analysis was performed on BHK-21 cell by adopting the MTT assay. The cytotoxicity activity of the 80% methanolic extract of leaves was evaluated by noticing the cell survival percentage (53.4%). Overall results of the present study showed that 80% methanolic leaves extracts of M. charantia possesses very good antioxidant, antimicrobial and cytotoxic properties.},
year = {2021}
}
TY - JOUR T1 - Phenolic Profile and Biological Properties of Momordica charantia AU - Zermina Khalid AU - Syeda Mona Hassan AU - Shahzad Sharif Mughal AU - Syed Khurram Hassan AU - Huma Hassan Y1 - 2021/03/26 PY - 2021 N1 - https://doi.org/10.11648/j.cbe.20210601.13 DO - 10.11648/j.cbe.20210601.13 T2 - Chemical and Biomolecular Engineering JF - Chemical and Biomolecular Engineering JO - Chemical and Biomolecular Engineering SP - 17 EP - 29 PB - Science Publishing Group SN - 2578-8884 UR - https://doi.org/10.11648/j.cbe.20210601.13 AB - M. charantia is an important medicinal plant belongs to family cucurbitaceae. It originates from India, Malasiya and is widely spread all over tropical, subtropical and warm temperate regions of the world. This research work has been designed to evaluate the antioxidant, antimicrobial and toxicological potential of M. charantia. The antifungal and antioxidant components of M. charantia leaves, seeds and peels were extracted by using four solvent systems (80% methanol, 80% ethanol, 100% methanol and 100% ethanol) andleaves presented maximum extract yield (22.7 g/100g DW) in 80% methanolic solvent system. Phytochemical analysis of M. Charantia leaves, seeds and peels extracts performed in terms of total phenolic and total flavonoid contents, showed that 80% methanolic leaves extract offered highest total phenolic contents (47.1 mg GAE/g DW), whereas80% ethanolic leaves gave maximum total flavonoid contents (67.3 mg CE/g DW). The phenolic contents were also analysed by HPLC. Antioxidant activity was determined by DPPH radical scavenging activity and measure of reducing power. Results revealed that 80% methanolic leaves extract showed highest radical scavenging activity and reducing potential. Antimicrobial activity of M. charantia leaves, seeds and peels was investigated by Disc Diffusion Method and Minimum Inhibitory Concentration (MIC). Results showed that 80% methanolic extract of leaves exhibited highest antibacterial and antifungal potential against P. multocida (30 mm DIZ) and A. paraciticus (28 mm DIZ), respectively. Cytotoxicity analysis was performed on BHK-21 cell by adopting the MTT assay. The cytotoxicity activity of the 80% methanolic extract of leaves was evaluated by noticing the cell survival percentage (53.4%). Overall results of the present study showed that 80% methanolic leaves extracts of M. charantia possesses very good antioxidant, antimicrobial and cytotoxic properties. VL - 6 IS - 1 ER -
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