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A Comparative Study on Reproductive Performance of Large White Boars Cloned by Somatic Cell Nuclear Transfer and Growth Performance of Their Progeny

Received: 2 September 2025     Accepted: 17 October 2025     Published: 28 November 2025
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Abstract

The success of cloning animal paves a new way to increase livestock production and conserve genetic resources. After the success of first cloned pig production, a large number of research reports related to cloned pig have been published. However, a few researches into reproductive ability of cloned pig were conducted. The evaluation of reproductive performance of cloning pig is of great importance in practical application for breeding and reproduction. The purpose of present study was to compare reproductive performance of cloned Large White boars with non-clones. First, cloned pigs were produced by somatic cell nuclear transfer (SCNT) technique. After confirmation of cloning success, two groups (clones versus non-clones) were created for a comparison. The semen quality and reproductive performance of pigs derived from SCNT were tested. Growth performance of their progeny was also compared. The results showed that there were no significant differences in semen quality and reproductive performance between clones and non-clones. The parameters related to growth performance such as weight and average daily gain were similar in both groups. To sum up, no remarkable differences were observed between clones and non-clones, indicating that SCNT technology could be applied not only to increase swine production but also to preserve and spread superior Large White pig species.

Published in Chemical and Biomolecular Engineering (Volume 10, Issue 4)
DOI 10.11648/j.cbe.20251004.11
Page(s) 49-53
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2025. Published by Science Publishing Group

Keywords

Somatic Cell Nuclear Transfer, Cloning, Reproductive Performance, Large White Boar

References
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[3] Unsong Shi, Liyao Xiao, Baohua Tan, Lvhua Luo, Zicong Li, Linjun Hong, Jie Yang, Gengyuan Cai, Enqin Zheng, Zhenfang Wu, Ting Gu. Comparative evaluation of production performances of cloned pigs derived from superior Duroc boars, Animal Reproduction Science, 244: 107049 (2022).
[4] Czernik, M., Iuso, D., Toschi, P., Khochbin, S., Loi, P. Remodeling somatic nuclei via exogenous expression of protamine 1 to create spermatid-like structures for somatic nuclear transfer, Nature Protocols, 11, 2170–2188 (2016).
[5] Liu, Y., Li, J., Lovendahl, P., Schmidt, M., Larsen, K., Callesen, H. In vitro manipulation techniques of porcine embryos: a meta-analysis related to transfers, pregnancies and piglets, Reproduction Fertility and Development, 27, 429–439 (2015).
[6] Samiec, M. The role of mitochondrial genome (mtDNA) in somatic and embryo cloning of mammals. A review, Journal of Animal and Feed Sciences, 14, 213–233 (2005).
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[9] Kui Hu, Qingran Kong, Zeping Zhao, Xinyu Lu, Biao Liu, Yutian Li, Hongbin Wang, Zhonghua Liu. Assessment of reproduction and growth performance of offspring derived from somatic cell cloned pigs, Animal Science Journal, 639–643 (2012).
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[11] Polejaeva IA, Chen SH, Vaught TD, Page RL, Mullins J, Ball, S, Dai Y, Boone J, Walker S, Ayares DL, Colman A, Campbell KH. Cloned pigs produced by nuclear transfer from adult somatic cells, Nature, 407, 86–90 (2000).
[12] Chen C. H., Jiang B. H., Huang S. Y., Yang T. S., Lee K. H., Tu C. F., Wu S. C. Genetic polymorphisms, growth performance, hematological parameters, serum enzymes, and reproductive characteristics in phenotypically normal Landrace boars produced by somatic cell nuclear transfer, Theriogenology, 80, 1088–1096 (2013).
[13] Shi, J., Tan, B., Luo, L., Li, Z., Hong, L., Yang, J., Cai, G., Zheng, E., Wu, Z., Gu, T. Assessment of the growth and reproductive performance of cloned pietrain Boars, Animals, 10 (2020).
[14] Shi, J., Zhou, R., Luo, L., Mai, R., Zeng, H., He, X., Liu, D., Zeng, F., Cai, G., Ji, H., Tang, F., Wang, Q., Wu, Z., Li, Z. Influence of embryo handling and transfer method on pig cloning efficiency, Animal Reproduction Science. 154, 121–127 (2015).
[15] Keefer, C. L. Artificial cloning of domestic animals. Proceedings of the National Academy of Sciences of the United States of AME, 112, 8874–8878 (2015).
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Cite This Article
  • APA Style

    Sin, C., Rim, S., Rim, C., Kim, R., To, K., et al. (2025). A Comparative Study on Reproductive Performance of Large White Boars Cloned by Somatic Cell Nuclear Transfer and Growth Performance of Their Progeny. Chemical and Biomolecular Engineering, 10(4), 49-53. https://doi.org/10.11648/j.cbe.20251004.11

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    ACS Style

    Sin, C.; Rim, S.; Rim, C.; Kim, R.; To, K., et al. A Comparative Study on Reproductive Performance of Large White Boars Cloned by Somatic Cell Nuclear Transfer and Growth Performance of Their Progeny. Chem. Biomol. Eng. 2025, 10(4), 49-53. doi: 10.11648/j.cbe.20251004.11

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    AMA Style

    Sin C, Rim S, Rim C, Kim R, To K, et al. A Comparative Study on Reproductive Performance of Large White Boars Cloned by Somatic Cell Nuclear Transfer and Growth Performance of Their Progeny. Chem Biomol Eng. 2025;10(4):49-53. doi: 10.11648/j.cbe.20251004.11

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  • @article{10.11648/j.cbe.20251004.11,
      author = {Chang-Gon Sin and Su-Chol Rim and Chang-Sin Rim and Ry-Chol Kim and Kwang-Il To and Chol-Min Kim and Won-Ju Hwang and Chong-Nam So and Hyok Ri and Myong-Il Ra and Hyok-Won Kim},
      title = {A Comparative Study on Reproductive Performance of Large White Boars Cloned by Somatic Cell Nuclear Transfer and Growth Performance of Their Progeny},
      journal = {Chemical and Biomolecular Engineering},
      volume = {10},
      number = {4},
      pages = {49-53},
      doi = {10.11648/j.cbe.20251004.11},
      url = {https://doi.org/10.11648/j.cbe.20251004.11},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.cbe.20251004.11},
      abstract = {The success of cloning animal paves a new way to increase livestock production and conserve genetic resources. After the success of first cloned pig production, a large number of research reports related to cloned pig have been published. However, a few researches into reproductive ability of cloned pig were conducted. The evaluation of reproductive performance of cloning pig is of great importance in practical application for breeding and reproduction. The purpose of present study was to compare reproductive performance of cloned Large White boars with non-clones. First, cloned pigs were produced by somatic cell nuclear transfer (SCNT) technique. After confirmation of cloning success, two groups (clones versus non-clones) were created for a comparison. The semen quality and reproductive performance of pigs derived from SCNT were tested. Growth performance of their progeny was also compared. The results showed that there were no significant differences in semen quality and reproductive performance between clones and non-clones. The parameters related to growth performance such as weight and average daily gain were similar in both groups. To sum up, no remarkable differences were observed between clones and non-clones, indicating that SCNT technology could be applied not only to increase swine production but also to preserve and spread superior Large White pig species.},
     year = {2025}
    }
    

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    T1  - A Comparative Study on Reproductive Performance of Large White Boars Cloned by Somatic Cell Nuclear Transfer and Growth Performance of Their Progeny
    AU  - Chang-Gon Sin
    AU  - Su-Chol Rim
    AU  - Chang-Sin Rim
    AU  - Ry-Chol Kim
    AU  - Kwang-Il To
    AU  - Chol-Min Kim
    AU  - Won-Ju Hwang
    AU  - Chong-Nam So
    AU  - Hyok Ri
    AU  - Myong-Il Ra
    AU  - Hyok-Won Kim
    Y1  - 2025/11/28
    PY  - 2025
    N1  - https://doi.org/10.11648/j.cbe.20251004.11
    DO  - 10.11648/j.cbe.20251004.11
    T2  - Chemical and Biomolecular Engineering
    JF  - Chemical and Biomolecular Engineering
    JO  - Chemical and Biomolecular Engineering
    SP  - 49
    EP  - 53
    PB  - Science Publishing Group
    SN  - 2578-8884
    UR  - https://doi.org/10.11648/j.cbe.20251004.11
    AB  - The success of cloning animal paves a new way to increase livestock production and conserve genetic resources. After the success of first cloned pig production, a large number of research reports related to cloned pig have been published. However, a few researches into reproductive ability of cloned pig were conducted. The evaluation of reproductive performance of cloning pig is of great importance in practical application for breeding and reproduction. The purpose of present study was to compare reproductive performance of cloned Large White boars with non-clones. First, cloned pigs were produced by somatic cell nuclear transfer (SCNT) technique. After confirmation of cloning success, two groups (clones versus non-clones) were created for a comparison. The semen quality and reproductive performance of pigs derived from SCNT were tested. Growth performance of their progeny was also compared. The results showed that there were no significant differences in semen quality and reproductive performance between clones and non-clones. The parameters related to growth performance such as weight and average daily gain were similar in both groups. To sum up, no remarkable differences were observed between clones and non-clones, indicating that SCNT technology could be applied not only to increase swine production but also to preserve and spread superior Large White pig species.
    VL  - 10
    IS  - 4
    ER  - 

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Author Information
  • Department of Integrated Animal Husbandry, Unjong Pig Test Farm, Pyongyang, DPR Korea

  • Department of Biotechnology, Institute of Chemistry and Biology, Pyongyang, DPR Korea

  • Department of Cloning, Institute of Animal Gene, Pyongyang, DPR Korea

  • Department of Cloning, Institute of Animal Gene, Pyongyang, DPR Korea

  • Department of Nanomaterials, Institute of Chemistry and Biology, Pyongyang, DPR Korea

  • Department of Integrated Animal Husbandry, Unjong Pig Test Farm, Pyongyang, DPR Korea

  • Department of Integrated Animal Husbandry, Unjong Pig Test Farm, Pyongyang, DPR Korea

  • Department of Integrated Animal Husbandry, Unjong Pig Test Farm, Pyongyang, DPR Korea

  • Department of Integrated Animal Husbandry, Unjong Pig Test Farm, Pyongyang, DPR Korea

  • Department of Integrated Animal Husbandry, Unjong Pig Test Farm, Pyongyang, DPR Korea

  • Department of Biotechnology, Institute of Chemistry and Biology, Pyongyang, DPR Korea

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