Organ Culture Model of Liver for the Study of Cancer Treatment for Hepatocellular Carcinoma

Torricelli Piera; Antonelli Francesco; Ferorelli Pasquale; De Martino Angelo; Shevchenko Anna; Siciliano Alberto; Beninati Simone

doi:doi:10.11648/j.crj.20160402.13

Organ Culture Model of Liver for the Study of Cancer Treatment for Hepatocellular Carcinoma

Torricelli Piera, Antonelli Francesco, Ferorelli Pasquale, De Martino Angelo, Shevchenko Anna, Siciliano Alberto, Beninati Simone

Published in Cancer Research Journal (Volume 4, Issue 2)

Received: 22 February 2016 Accepted: 1 March 2016 Published: 16 March 2016

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Abstract

The liver, the largest organ of the human body, is a multifunctional organ with various metabolic activities that plays a fundamental role in maintaining the body and in sustaining life. Although the liver has great regenerative capacity and recovery, the damage caused by chronic diseases such as cancer or viral infections can lead to permanent loss of liver function. Studies on the mechanism of liver disease, have focused on the selection of cell and tissue culture techniques, including strategies based on in vitro models. The organ culture is a promising tool for the study of liver diseases, because it can mimic the complex of the microenvironment in vivo using a three-dimensional model of human liver tissue. These models allow a better study of the specific functions of the liver. In this context, we have analyzed the development of a hepatocarcinoma, obtained by inoculating a murine hepatocarcinoma cell line, Hepa 1/A1s, in the liver of 10 mice of the strain C57BL / 6. After 20 days from the inoculation, the portion of liver invaded by the tumor was removed from the animals and cultured. A group of 5 liver explants were used as a control and other 5 explants were cultured for 4 weeks in a complete medium containing 10% Citozym, a food supplement with reported antioxidant properties. The cancer-invaded hepatic lobes, treated with Citozym, showed a clear reduction of the weight and the volume of the hepatic tumors, when compared with the control explants.

Published in	Cancer Research Journal (Volume 4, Issue 2)
DOI	10.11648/j.crj.20160402.13
Page(s)	37-42
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2016. Published by Science Publishing Group

Keywords

Food Supplements, Antioxidants, Hepatocarcinoma, Organ Cultures, Oxidative Stress

References

[1]	Cardin R, Piciocchi M, Bortolami M, Kotsafti A, Barzon L, Lavezzo, SinigagliaA, Rodriguez-Castro KI, Rugge M, Farinati F. Oxidative damage in the progression of chronic liver disease to hepatocellular carcinoma: an intricate pathway. WorldJ Gastroenterol. 2014; 20(12): 3078-3086.
[2]	Langley, G. La validité de l’expérimentation animale en recherche médicale. Revue Semestrielle de Droit Animalier – RSDA. 2009; 1: 161-168.
[3]	Paixão RL, Schramm FR. Ethics nd animal experimentation: what is debated? Cad Saude Publica. 1999; 15 Suppl 1: 99-110.
[4]	Edelman LB, Eddy JA, Price ND. In silico models of cancer. Wiley Interdiscip Rev Syst Biol Med. 2010; 2(4): 438-459.
[5]	Carranza-Torres IE, Guzmán-Delgado NE, Cornado-Martínez C, Bañuelos-García JI, Viveros-Valdez E, Morán-Martínez J, Carranza-Rosales P. Organotypic culture of breast tumor explants as a multicellular system for the screening of natural compounds with antineoplastic potential. Biomed Res Int. 2015: 618021.
[6]	Sosa V., Moliné T., Somoza R., Paciucci R., Kondoh H., L Leonart M. E. Oxidative stress and cancer: an overview. Ageing Research Reviews. 2013; 12(1): 376–390.
[7]	Howarth C, Gleeson P, Attwell D. Updated energy budgets for neural computation in the neocortex and cerebellum. J. Cereb. Blood Flow Metab.2012; 32 (7): 1222–1232.
[8]	Wang HY, O'Doherty GA. Modulators of Na/K-ATPase: a patent review. Expert Opin Ther Pat. 2012; 22(6): 587-605.
[9]	Antonelli F and Beninati S. Enhanced survival of B16-F10 melanoma tumour-bearing C57BL6/N mice treated with a mixture of antioxidants in: Recent Res Devel in Life Sci. Research Signpost, Trivandrum India.2011; 5: 51-60.
[10]	Greene AK, Puder M. Partial hepatectomy in the mouse: technique and perioperative management. J Invest Surg. 2003; 16(2): 99-102.
[11]	Frith CH, Ward JM, Turusov VS. Tumours of the liver. IARCSci Publ. 1994; (111): 223–269.
[12]	Moreno P, Salvadó V. Determination of eight water- and fat-soluble vitamins in multi-vitamin pharmaceutical formulations by high-performance liquid chromatography. J Chromatogr A. 2000; 870(1-2): 207-215.
[13]	Romero Rodriguez MA, Vazquez Oderiz ML, Lopez Hernandez J, Simal Lozano J. Determination of vitamin C and organic acids in various fruits by HPLC. J Chromatogr Sci. 1992; 30(11): 433-437.
[14]	Spínola V, Llorent-Martínez EJ, Castilho PC. Determination of vitamin C in foods: current state of method validation. J Chromatogr A. 2014; 1369: 2-17.
[15]	Bradford, M. M. Rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding, Anal. Biochem. 1976; 72: 248–254.
[16]	Maeda S, Kamata H, Luo JL, Leffert H, Karin M, et al. IKK beta couples hepatocyte death to cytokine-driven compensatory proliferation that promotes chemical hepatocarcinogenesis. Cell. 2005; 121: 977–990.
[17]	Kroger A, Ortmann D, Krohne TU, et al. Growth suppression of the hepatocellular carcinoma cell line Hepa1-6 by an activatable interferon regulatory factor-1 in mice. Cancer Res. 2001; 61: 2609–2617.
[18]	Torricelli P, Ferorelli P, De Martino A, Antonelli F, A. Shevchenko A, Beninati S. Regression of Carotid Plaques in Individuals at Low-to-intermediate Cardiovascular Risk Treated with Citozym and Propulzym. European Journal of Preventive Medicine. 2014; 2 (3): 33-37.
[19]	Torricelli P., Ferorelli P., De Martino A., Antonelli F., Beninati S. The influence of preventive multiple micronutrients supplementation on liver steatosis in high-cholesterol fed C57BL6/N mice. American J Life Sciences, 2013; 1(2): 55-60.
[20]	Torricelli P. Ferorelli P., De Martino A., Antonelli F., Beninati S. Preventive effects of a mixture of micronutrients with antioxidative properties on experimentally induced prostate hyperplasia. American J. Life Sciences, 2013; 1(1): 22-26.
[21]	Torricelli P., Antonelli F., Ferorelli P., De Martino A., Shevchenko A., Beninati S. Antiproliferative activity of a dietary supplement on estrogen receptor positive and negative human breast adenocarcinoma cell lines. Cancer Research J 2014; 2(2): 29-32.
[22]	Ozben T. Antioxidant supplementation on cancer risk and during cancer therapy: an update. Current Topics in Medicinal Chemistry. 2015; 15(2): 170–178.
[23]	Radimer K., Bindewald B., Hughes J., Ervin B., Swanson C., Picciano M. F. Dietary supplement use by US adults: data from the National Health and Nutrition Examination Survey, 1999-2000. The American Journal of Epidemiology. 2004; 160(4): 339–349.
[24]	Bennett L. L., Rojas S., Seefeldt T. Role of antioxidants in the prevention of cancer. Journal of Experimental and Clinical Medicine. 2012; 4(4): 215–222.
[25]	Undurti N. Das. Pyruvate is an endogenous anti-inflammatory and anti-oxidant molecule Med Sci Monit, 2006; 12(5): RA79-84.
[26]	Matsumoto Y, Kittaka A, Chen TC. 19- Norvitamin D analogs for breast cancer therapy. Can J Physiol Pharmacol. 2015; 93(5): 333-348.
[27]	Gao P, Zhang H, Dinavahi R, Li F, Xiang Y, Raman V, Bhujwalla ZM, Felsher DW, Cheng L, Pevsner J, Lee LA, Semenza GL, Dang CV. HIF-dependent antitumorigenic effect of antioxidants in vivo. Cancer Cell. 2007; 12(3): 230-238.
[28]	Lewisohn R. Leuchtenberger C., Leuchtenberger R Laszlo D., and Bloch, K. Prevention of Tumor Growth (Carcinoma 2163) by Intravenous Injections of Yeast and Vitamins. Science 1941; 94: 70-71.
[29]	Sanjoaquin M. A., Allen N., Couto E., Roddam A. W. and Key T. J. Folate intake and colorectal cancer risk: a me ta-analytical approach. International journal of cancer. Journal international du cancer. 2005; 113, 825–828.

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APA Style

Torricelli Piera, Antonelli Francesco, Ferorelli Pasquale, De Martino Angelo, Shevchenko Anna, et al. (2016). Organ Culture Model of Liver for the Study of Cancer Treatment for Hepatocellular Carcinoma. Cancer Research Journal, 4(2), 37-42. https://doi.org/10.11648/j.crj.20160402.13

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ACS Style

Torricelli Piera; Antonelli Francesco; Ferorelli Pasquale; De Martino Angelo; Shevchenko Anna, et al. Organ Culture Model of Liver for the Study of Cancer Treatment for Hepatocellular Carcinoma. Cancer Res. J. 2016, 4(2), 37-42. doi: 10.11648/j.crj.20160402.13

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AMA Style

Torricelli Piera, Antonelli Francesco, Ferorelli Pasquale, De Martino Angelo, Shevchenko Anna, et al. Organ Culture Model of Liver for the Study of Cancer Treatment for Hepatocellular Carcinoma. Cancer Res J. 2016;4(2):37-42. doi: 10.11648/j.crj.20160402.13

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@article{10.11648/j.crj.20160402.13,
  author = {Torricelli Piera and Antonelli Francesco and Ferorelli Pasquale and De Martino Angelo and Shevchenko Anna and Siciliano Alberto and Beninati Simone},
  title = {Organ Culture Model of Liver for the Study of Cancer Treatment for Hepatocellular Carcinoma},
  journal = {Cancer Research Journal},
  volume = {4},
  number = {2},
  pages = {37-42},
  doi = {10.11648/j.crj.20160402.13},
  url = {https://doi.org/10.11648/j.crj.20160402.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.crj.20160402.13},
  abstract = {The liver, the largest organ of the human body, is a multifunctional organ with various metabolic activities that plays a fundamental role in maintaining the body and in sustaining life. Although the liver has great regenerative capacity and recovery, the damage caused by chronic diseases such as cancer or viral infections can lead to permanent loss of liver function. Studies on the mechanism of liver disease, have focused on the selection of cell and tissue culture techniques, including strategies based on in vitro models. The organ culture is a promising tool for the study of liver diseases, because it can mimic the complex of the microenvironment in vivo using a three-dimensional model of human liver tissue. These models allow a better study of the specific functions of the liver. In this context, we have analyzed the development of a hepatocarcinoma, obtained by inoculating a murine hepatocarcinoma cell line, Hepa 1/A1s, in the liver of 10 mice of the strain C57BL / 6. After 20 days from the inoculation, the portion of liver invaded by the tumor was removed from the animals and cultured. A group of 5 liver explants were used as a control and other 5 explants were cultured for 4 weeks in a complete medium containing 10% Citozym, a food supplement with reported antioxidant properties. The cancer-invaded hepatic lobes, treated with Citozym, showed a clear reduction of the weight and the volume of the hepatic tumors, when compared with the control explants.},
 year = {2016}
}

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TY  - JOUR
T1  - Organ Culture Model of Liver for the Study of Cancer Treatment for Hepatocellular Carcinoma
AU  - Torricelli Piera
AU  - Antonelli Francesco
AU  - Ferorelli Pasquale
AU  - De Martino Angelo
AU  - Shevchenko Anna
AU  - Siciliano Alberto
AU  - Beninati Simone
Y1  - 2016/03/16
PY  - 2016
N1  - https://doi.org/10.11648/j.crj.20160402.13
DO  - 10.11648/j.crj.20160402.13
T2  - Cancer Research Journal
JF  - Cancer Research Journal
JO  - Cancer Research Journal
SP  - 37
EP  - 42
PB  - Science Publishing Group
SN  - 2330-8214
UR  - https://doi.org/10.11648/j.crj.20160402.13
AB  - The liver, the largest organ of the human body, is a multifunctional organ with various metabolic activities that plays a fundamental role in maintaining the body and in sustaining life. Although the liver has great regenerative capacity and recovery, the damage caused by chronic diseases such as cancer or viral infections can lead to permanent loss of liver function. Studies on the mechanism of liver disease, have focused on the selection of cell and tissue culture techniques, including strategies based on in vitro models. The organ culture is a promising tool for the study of liver diseases, because it can mimic the complex of the microenvironment in vivo using a three-dimensional model of human liver tissue. These models allow a better study of the specific functions of the liver. In this context, we have analyzed the development of a hepatocarcinoma, obtained by inoculating a murine hepatocarcinoma cell line, Hepa 1/A1s, in the liver of 10 mice of the strain C57BL / 6. After 20 days from the inoculation, the portion of liver invaded by the tumor was removed from the animals and cultured. A group of 5 liver explants were used as a control and other 5 explants were cultured for 4 weeks in a complete medium containing 10% Citozym, a food supplement with reported antioxidant properties. The cancer-invaded hepatic lobes, treated with Citozym, showed a clear reduction of the weight and the volume of the hepatic tumors, when compared with the control explants.
VL  - 4
IS  - 2
ER  -

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Author Information

Torricelli Piera

Department SPES, University of Molise, Campobasso, Italy
Antonelli Francesco

Department of Biology, University of Tor Vergata, Rome, Italy
Ferorelli Pasquale

Department of Biology, University of Tor Vergata, Rome, Italy
De Martino Angelo

Department of Biology, University of Tor Vergata, Rome, Italy
Shevchenko Anna

People’s Friendship University of Russia, Moscow, Russia
Siciliano Alberto

Department of Biology, University of Tor Vergata, Rome, Italy
Beninati Simone

Department of Biology, University of Tor Vergata, Rome, Italy

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APA Style

Torricelli Piera, Antonelli Francesco, Ferorelli Pasquale, De Martino Angelo, Shevchenko Anna, et al. (2016). Organ Culture Model of Liver for the Study of Cancer Treatment for Hepatocellular Carcinoma. Cancer Research Journal, 4(2), 37-42. https://doi.org/10.11648/j.crj.20160402.13

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ACS Style

Torricelli Piera; Antonelli Francesco; Ferorelli Pasquale; De Martino Angelo; Shevchenko Anna, et al. Organ Culture Model of Liver for the Study of Cancer Treatment for Hepatocellular Carcinoma. Cancer Res. J. 2016, 4(2), 37-42. doi: 10.11648/j.crj.20160402.13

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AMA Style

Torricelli Piera, Antonelli Francesco, Ferorelli Pasquale, De Martino Angelo, Shevchenko Anna, et al. Organ Culture Model of Liver for the Study of Cancer Treatment for Hepatocellular Carcinoma. Cancer Res J. 2016;4(2):37-42. doi: 10.11648/j.crj.20160402.13

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@article{10.11648/j.crj.20160402.13,
  author = {Torricelli Piera and Antonelli Francesco and Ferorelli Pasquale and De Martino Angelo and Shevchenko Anna and Siciliano Alberto and Beninati Simone},
  title = {Organ Culture Model of Liver for the Study of Cancer Treatment for Hepatocellular Carcinoma},
  journal = {Cancer Research Journal},
  volume = {4},
  number = {2},
  pages = {37-42},
  doi = {10.11648/j.crj.20160402.13},
  url = {https://doi.org/10.11648/j.crj.20160402.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.crj.20160402.13},
  abstract = {The liver, the largest organ of the human body, is a multifunctional organ with various metabolic activities that plays a fundamental role in maintaining the body and in sustaining life. Although the liver has great regenerative capacity and recovery, the damage caused by chronic diseases such as cancer or viral infections can lead to permanent loss of liver function. Studies on the mechanism of liver disease, have focused on the selection of cell and tissue culture techniques, including strategies based on in vitro models. The organ culture is a promising tool for the study of liver diseases, because it can mimic the complex of the microenvironment in vivo using a three-dimensional model of human liver tissue. These models allow a better study of the specific functions of the liver. In this context, we have analyzed the development of a hepatocarcinoma, obtained by inoculating a murine hepatocarcinoma cell line, Hepa 1/A1s, in the liver of 10 mice of the strain C57BL / 6. After 20 days from the inoculation, the portion of liver invaded by the tumor was removed from the animals and cultured. A group of 5 liver explants were used as a control and other 5 explants were cultured for 4 weeks in a complete medium containing 10% Citozym, a food supplement with reported antioxidant properties. The cancer-invaded hepatic lobes, treated with Citozym, showed a clear reduction of the weight and the volume of the hepatic tumors, when compared with the control explants.},
 year = {2016}
}

Copy | Download

TY  - JOUR
T1  - Organ Culture Model of Liver for the Study of Cancer Treatment for Hepatocellular Carcinoma
AU  - Torricelli Piera
AU  - Antonelli Francesco
AU  - Ferorelli Pasquale
AU  - De Martino Angelo
AU  - Shevchenko Anna
AU  - Siciliano Alberto
AU  - Beninati Simone
Y1  - 2016/03/16
PY  - 2016
N1  - https://doi.org/10.11648/j.crj.20160402.13
DO  - 10.11648/j.crj.20160402.13
T2  - Cancer Research Journal
JF  - Cancer Research Journal
JO  - Cancer Research Journal
SP  - 37
EP  - 42
PB  - Science Publishing Group
SN  - 2330-8214
UR  - https://doi.org/10.11648/j.crj.20160402.13
AB  - The liver, the largest organ of the human body, is a multifunctional organ with various metabolic activities that plays a fundamental role in maintaining the body and in sustaining life. Although the liver has great regenerative capacity and recovery, the damage caused by chronic diseases such as cancer or viral infections can lead to permanent loss of liver function. Studies on the mechanism of liver disease, have focused on the selection of cell and tissue culture techniques, including strategies based on in vitro models. The organ culture is a promising tool for the study of liver diseases, because it can mimic the complex of the microenvironment in vivo using a three-dimensional model of human liver tissue. These models allow a better study of the specific functions of the liver. In this context, we have analyzed the development of a hepatocarcinoma, obtained by inoculating a murine hepatocarcinoma cell line, Hepa 1/A1s, in the liver of 10 mice of the strain C57BL / 6. After 20 days from the inoculation, the portion of liver invaded by the tumor was removed from the animals and cultured. A group of 5 liver explants were used as a control and other 5 explants were cultured for 4 weeks in a complete medium containing 10% Citozym, a food supplement with reported antioxidant properties. The cancer-invaded hepatic lobes, treated with Citozym, showed a clear reduction of the weight and the volume of the hepatic tumors, when compared with the control explants.
VL  - 4
IS  - 2
ER  -

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