In this study, specimens of plasma were collected from 96 from patient diagnosed with cancer from Al Karama teaching hospital. Also plasma collected from 25 healthy individual as control. Cell-free DNA (cfDNA) was extracted from 1ml plasma using high pure viral Nucleic Acid Kit as an alternative to high expensive cfDNA kit. cfDNA extracted according to the manufacturer’s instructions, but without the use of carrier RNA. This represent the first recorded method of using viral nucleic acid extraction kit for collection of cell free DNA. Results indicate that all cancer samples show significant increase of cfDNA in compare with control (p 0.01). and this dramatic increase in DNA concentration provide good indicator about health condition. DNA integrity have been checked depending on amplification of Arthrobacter luteus (Alu sequence). Both small (115) and large (247) large Alu stretches have amplified using RT PCR using SYBR green dye. Results shows that integrity of DNA extracted from patients and control are suitable for any further molecular investigation, and short Alu repeats are much less abundant in health individual in compare with cancer patient of all cancer types under study. Depending on these results one can conclude that almost all DNA obtained from normal patients is finely fragmented due to apoptosis action and that’s why give very little positive. results in compare with corresponding cancer patient which release large DNA fragments resulted from necrosis and NK/Tc cells activity, which is amplifiable more efficiently. In this work another molecular study conducted to investigate the cancer specific sequences. Since we deals with different cancers type in this study, two types of prostate, ovary, testis expressed protein (POTE Ankyrine) were tested 2α and 2β. Results shows that almost all healthy group were negative for specific POTE test while cancer patient samples are positive for POTE specific amplification. This results are promising since it could be developed to be a building block of cfDNA cancer tests.
| Published in | Cancer Research Journal (Volume 6, Issue 1) |
| DOI | 10.11648/j.crj.20180601.14 |
| Page(s) | 20-25 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2018. Published by Science Publishing Group |
cf-DNA, Alu Sequences, POTE Ankyrine Gene
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APA Style
Ahmed Darweesh Jabbar, Safa Abdul Ilah Faraj, Tariq Dawood Salman, Sarah Majed Kadhum, Zainab Dhiyaa Jabuori. (2018). Alu and Poet Ankyrine Detection and Quantization in Cell Free Dna of Cancer Patients. Cancer Research Journal, 6(1), 20-25. https://doi.org/10.11648/j.crj.20180601.14
ACS Style
Ahmed Darweesh Jabbar; Safa Abdul Ilah Faraj; Tariq Dawood Salman; Sarah Majed Kadhum; Zainab Dhiyaa Jabuori. Alu and Poet Ankyrine Detection and Quantization in Cell Free Dna of Cancer Patients. Cancer Res. J. 2018, 6(1), 20-25. doi: 10.11648/j.crj.20180601.14
AMA Style
Ahmed Darweesh Jabbar, Safa Abdul Ilah Faraj, Tariq Dawood Salman, Sarah Majed Kadhum, Zainab Dhiyaa Jabuori. Alu and Poet Ankyrine Detection and Quantization in Cell Free Dna of Cancer Patients. Cancer Res J. 2018;6(1):20-25. doi: 10.11648/j.crj.20180601.14
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Download@article{10.11648/j.crj.20180601.14,
author = {Ahmed Darweesh Jabbar and Safa Abdul Ilah Faraj and Tariq Dawood Salman and Sarah Majed Kadhum and Zainab Dhiyaa Jabuori},
title = {Alu and Poet Ankyrine Detection and Quantization in Cell Free Dna of Cancer Patients},
journal = {Cancer Research Journal},
volume = {6},
number = {1},
pages = {20-25},
doi = {10.11648/j.crj.20180601.14},
url = {https://doi.org/10.11648/j.crj.20180601.14},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.crj.20180601.14},
abstract = {In this study, specimens of plasma were collected from 96 from patient diagnosed with cancer from Al Karama teaching hospital. Also plasma collected from 25 healthy individual as control. Cell-free DNA (cfDNA) was extracted from 1ml plasma using high pure viral Nucleic Acid Kit as an alternative to high expensive cfDNA kit. cfDNA extracted according to the manufacturer’s instructions, but without the use of carrier RNA. This represent the first recorded method of using viral nucleic acid extraction kit for collection of cell free DNA. Results indicate that all cancer samples show significant increase of cfDNA in compare with control (p 0.01). and this dramatic increase in DNA concentration provide good indicator about health condition. DNA integrity have been checked depending on amplification of Arthrobacter luteus (Alu sequence). Both small (115) and large (247) large Alu stretches have amplified using RT PCR using SYBR green dye. Results shows that integrity of DNA extracted from patients and control are suitable for any further molecular investigation, and short Alu repeats are much less abundant in health individual in compare with cancer patient of all cancer types under study. Depending on these results one can conclude that almost all DNA obtained from normal patients is finely fragmented due to apoptosis action and that’s why give very little positive. results in compare with corresponding cancer patient which release large DNA fragments resulted from necrosis and NK/Tc cells activity, which is amplifiable more efficiently. In this work another molecular study conducted to investigate the cancer specific sequences. Since we deals with different cancers type in this study, two types of prostate, ovary, testis expressed protein (POTE Ankyrine) were tested 2α and 2β. Results shows that almost all healthy group were negative for specific POTE test while cancer patient samples are positive for POTE specific amplification. This results are promising since it could be developed to be a building block of cfDNA cancer tests.},
year = {2018}
}
TY - JOUR T1 - Alu and Poet Ankyrine Detection and Quantization in Cell Free Dna of Cancer Patients AU - Ahmed Darweesh Jabbar AU - Safa Abdul Ilah Faraj AU - Tariq Dawood Salman AU - Sarah Majed Kadhum AU - Zainab Dhiyaa Jabuori Y1 - 2018/03/05 PY - 2018 N1 - https://doi.org/10.11648/j.crj.20180601.14 DO - 10.11648/j.crj.20180601.14 T2 - Cancer Research Journal JF - Cancer Research Journal JO - Cancer Research Journal SP - 20 EP - 25 PB - Science Publishing Group SN - 2330-8214 UR - https://doi.org/10.11648/j.crj.20180601.14 AB - In this study, specimens of plasma were collected from 96 from patient diagnosed with cancer from Al Karama teaching hospital. Also plasma collected from 25 healthy individual as control. Cell-free DNA (cfDNA) was extracted from 1ml plasma using high pure viral Nucleic Acid Kit as an alternative to high expensive cfDNA kit. cfDNA extracted according to the manufacturer’s instructions, but without the use of carrier RNA. This represent the first recorded method of using viral nucleic acid extraction kit for collection of cell free DNA. Results indicate that all cancer samples show significant increase of cfDNA in compare with control (p 0.01). and this dramatic increase in DNA concentration provide good indicator about health condition. DNA integrity have been checked depending on amplification of Arthrobacter luteus (Alu sequence). Both small (115) and large (247) large Alu stretches have amplified using RT PCR using SYBR green dye. Results shows that integrity of DNA extracted from patients and control are suitable for any further molecular investigation, and short Alu repeats are much less abundant in health individual in compare with cancer patient of all cancer types under study. Depending on these results one can conclude that almost all DNA obtained from normal patients is finely fragmented due to apoptosis action and that’s why give very little positive. results in compare with corresponding cancer patient which release large DNA fragments resulted from necrosis and NK/Tc cells activity, which is amplifiable more efficiently. In this work another molecular study conducted to investigate the cancer specific sequences. Since we deals with different cancers type in this study, two types of prostate, ovary, testis expressed protein (POTE Ankyrine) were tested 2α and 2β. Results shows that almost all healthy group were negative for specific POTE test while cancer patient samples are positive for POTE specific amplification. This results are promising since it could be developed to be a building block of cfDNA cancer tests. VL - 6 IS - 1 ER -
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