N-Nitrosation of Dimethylamine by Bacillus cereus Isolated from Fermented Palm Sap (Elaeis guineensis)

Adeleke G. E.; Adedosu O. T.; Oyewo E. B.; Fatoki J. O.; Abioye D. H.; Ishola A. A.; Maduagwu E. N.

doi:doi:10.11648/j.crj.20180602.13

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N-Nitrosation of Dimethylamine by Bacillus cereus Isolated from Fermented Palm Sap (Elaeis guineensis)

Adeleke G. E., Adedosu O. T., Oyewo E. B., Fatoki J. O., Abioye D. H., Ishola A. A., Maduagwu E. N.

Published in Cancer Research Journal (Volume 6, Issue 2)

Received: 31 December 2017 Accepted: 5 March 2018 Published: 27 March 2018

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Abstract

This study investigated the nitrosating activities of intact cells and cell fractions of Bacillus cereus isolated from fermented palm wine (Elaeis guineensis) during incubation with dimethylamine and nitrite or nitrate. The pH values of the incubation mixtures were 7.2 ± 0.07 and 7.1 ± 0.07 in the presence of nitrite and nitrate, respectively. The N-Nitrosodimethylamine (NDMA) level in cell debris was significantly higher compared with cell extract, cell suspension and sterile controls. The time-course of NDMA formation in cell debris showed a significant (p < 0.05) early decline followed by an increase. The kinetic data obtained from Lineweaver-Burk plots of NDMA formation showed that Michaelis -Menten constant (K_m) value was 37.5% lower, while initial velocity (V_max) was 20.0% higher in cell debris relative to cell extract, when a fixed DMA concentration was used against varying nitrite concentrations. However, at a fixed nitrite concentration against varying DMA concentration, K_m value was 44.4% lower, while V_max was 166.7% higher in cell debris compared with cell extract. Investigation of nitrosation mechanism showed that V_max values in the cell debris were 41.7, 50.0 and 55.6 μmol NDMA/mg protein, while the values were 34.5, 37.0 and 43.5 μmol NDMA/mg protein in cell extract for the respective three nitrite concentrations. This study has shown that intact Bacillus cereus cells catalyzed nitrosation of dimethylamine at near neutral pH, and the nitrosation, which was higher in cell debris than cell extract of the bacterium, followed a sequential mechanism of enzyme catalysis.

Published in	Cancer Research Journal (Volume 6, Issue 2)
DOI	10.11648/j.crj.20180602.13
Page(s)	51-61
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2018. Published by Science Publishing Group

Keywords

Bacillus cereus, Dimethylamine, Enzyme Mechanism, Palm Wine, N-Nitrosation

References

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Adeleke G. E., Adedosu O. T., Oyewo E. B., Fatoki J. O., Abioye D. H., et al. (2018). N-Nitrosation of Dimethylamine by Bacillus cereus Isolated from Fermented Palm Sap (Elaeis guineensis). Cancer Research Journal, 6(2), 51-61. https://doi.org/10.11648/j.crj.20180602.13

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ACS Style

Adeleke G. E.; Adedosu O. T.; Oyewo E. B.; Fatoki J. O.; Abioye D. H., et al. N-Nitrosation of Dimethylamine by Bacillus cereus Isolated from Fermented Palm Sap (Elaeis guineensis). Cancer Res. J. 2018, 6(2), 51-61. doi: 10.11648/j.crj.20180602.13

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AMA Style

Adeleke G. E., Adedosu O. T., Oyewo E. B., Fatoki J. O., Abioye D. H., et al. N-Nitrosation of Dimethylamine by Bacillus cereus Isolated from Fermented Palm Sap (Elaeis guineensis). Cancer Res J. 2018;6(2):51-61. doi: 10.11648/j.crj.20180602.13

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@article{10.11648/j.crj.20180602.13,
  author = {Adeleke G. E. and Adedosu O. T. and Oyewo E. B. and Fatoki J. O. and Abioye D. H. and Ishola A. A. and Maduagwu E. N.},
  title = {N-Nitrosation of Dimethylamine by Bacillus cereus Isolated from Fermented Palm Sap (Elaeis guineensis)},
  journal = {Cancer Research Journal},
  volume = {6},
  number = {2},
  pages = {51-61},
  doi = {10.11648/j.crj.20180602.13},
  url = {https://doi.org/10.11648/j.crj.20180602.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.crj.20180602.13},
  abstract = {This study investigated the nitrosating activities of intact cells and cell fractions of Bacillus cereus isolated from fermented palm wine (Elaeis guineensis) during incubation with dimethylamine and nitrite or nitrate. The pH values of the incubation mixtures were 7.2 ± 0.07 and 7.1 ± 0.07 in the presence of nitrite and nitrate, respectively. The N-Nitrosodimethylamine (NDMA) level in cell debris was significantly higher compared with cell extract, cell suspension and sterile controls. The time-course of NDMA formation in cell debris showed a significant (p m) value was 37.5% lower, while initial velocity (Vmax) was 20.0% higher in cell debris relative to cell extract, when a fixed DMA concentration was used against varying nitrite concentrations. However, at a fixed nitrite concentration against varying DMA concentration, Km value was 44.4% lower, while Vmax was 166.7% higher in cell debris compared with cell extract. Investigation of nitrosation mechanism showed that Vmax values in the cell debris were 41.7, 50.0 and 55.6 μmol NDMA/mg protein, while the values were 34.5, 37.0 and 43.5 μmol NDMA/mg protein in cell extract for the respective three nitrite concentrations. This study has shown that intact Bacillus cereus cells catalyzed nitrosation of dimethylamine at near neutral pH, and the nitrosation, which was higher in cell debris than cell extract of the bacterium, followed a sequential mechanism of enzyme catalysis.},
 year = {2018}
}

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TY - JOUR
T1 - N-Nitrosation of Dimethylamine by Bacillus cereus Isolated from Fermented Palm Sap (Elaeis guineensis)
AU - Adeleke G. E.
AU - Adedosu O. T.
AU - Oyewo E. B.
AU - Fatoki J. O.
AU - Abioye D. H.
AU - Ishola A. A.
AU - Maduagwu E. N.
Y1 - 2018/03/27
PY - 2018
N1 - https://doi.org/10.11648/j.crj.20180602.13
DO - 10.11648/j.crj.20180602.13
T2 - Cancer Research Journal
JF - Cancer Research Journal
JO - Cancer Research Journal
SP - 51
EP - 61
PB - Science Publishing Group
SN - 2330-8214
UR - https://doi.org/10.11648/j.crj.20180602.13
AB - This study investigated the nitrosating activities of intact cells and cell fractions of Bacillus cereus isolated from fermented palm wine (Elaeis guineensis) during incubation with dimethylamine and nitrite or nitrate. The pH values of the incubation mixtures were 7.2 ± 0.07 and 7.1 ± 0.07 in the presence of nitrite and nitrate, respectively. The N-Nitrosodimethylamine (NDMA) level in cell debris was significantly higher compared with cell extract, cell suspension and sterile controls. The time-course of NDMA formation in cell debris showed a significant (p m) value was 37.5% lower, while initial velocity (Vmax) was 20.0% higher in cell debris relative to cell extract, when a fixed DMA concentration was used against varying nitrite concentrations. However, at a fixed nitrite concentration against varying DMA concentration, Km value was 44.4% lower, while Vmax was 166.7% higher in cell debris compared with cell extract. Investigation of nitrosation mechanism showed that Vmax values in the cell debris were 41.7, 50.0 and 55.6 μmol NDMA/mg protein, while the values were 34.5, 37.0 and 43.5 μmol NDMA/mg protein in cell extract for the respective three nitrite concentrations. This study has shown that intact Bacillus cereus cells catalyzed nitrosation of dimethylamine at near neutral pH, and the nitrosation, which was higher in cell debris than cell extract of the bacterium, followed a sequential mechanism of enzyme catalysis.
VL - 6
IS - 2
ER -

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Author Information

Adeleke G. E.

Department of Biochemistry, Ladoke Akintola University of Technology, Ogbomoso, Nigeria
Adedosu O. T.

Department of Biochemistry, Ladoke Akintola University of Technology, Ogbomoso, Nigeria
Oyewo E. B.

Department of Biochemistry, Ladoke Akintola University of Technology, Ogbomoso, Nigeria
Fatoki J. O.

Department of Biochemistry, Ladoke Akintola University of Technology, Ogbomoso, Nigeria
Abioye D. H.

Department of Biochemistry, Ladoke Akintola University of Technology, Ogbomoso, Nigeria
Ishola A. A.

Department of Biochemistry, Ladoke Akintola University of Technology, Ogbomoso, Nigeria
Maduagwu E. N.

Department of Biological Sciences, Biochemistry Unit, Covenant University, Ota, Nigeria

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Adeleke G. E., Adedosu O. T., Oyewo E. B., Fatoki J. O., Abioye D. H., et al. (2018). N-Nitrosation of Dimethylamine by Bacillus cereus Isolated from Fermented Palm Sap (Elaeis guineensis). Cancer Research Journal, 6(2), 51-61. https://doi.org/10.11648/j.crj.20180602.13

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Adeleke G. E.; Adedosu O. T.; Oyewo E. B.; Fatoki J. O.; Abioye D. H., et al. N-Nitrosation of Dimethylamine by Bacillus cereus Isolated from Fermented Palm Sap (Elaeis guineensis). Cancer Res. J. 2018, 6(2), 51-61. doi: 10.11648/j.crj.20180602.13

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AMA Style

Adeleke G. E., Adedosu O. T., Oyewo E. B., Fatoki J. O., Abioye D. H., et al. N-Nitrosation of Dimethylamine by Bacillus cereus Isolated from Fermented Palm Sap (Elaeis guineensis). Cancer Res J. 2018;6(2):51-61. doi: 10.11648/j.crj.20180602.13

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@article{10.11648/j.crj.20180602.13,
  author = {Adeleke G. E. and Adedosu O. T. and Oyewo E. B. and Fatoki J. O. and Abioye D. H. and Ishola A. A. and Maduagwu E. N.},
  title = {N-Nitrosation of Dimethylamine by Bacillus cereus Isolated from Fermented Palm Sap (Elaeis guineensis)},
  journal = {Cancer Research Journal},
  volume = {6},
  number = {2},
  pages = {51-61},
  doi = {10.11648/j.crj.20180602.13},
  url = {https://doi.org/10.11648/j.crj.20180602.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.crj.20180602.13},
  abstract = {This study investigated the nitrosating activities of intact cells and cell fractions of Bacillus cereus isolated from fermented palm wine (Elaeis guineensis) during incubation with dimethylamine and nitrite or nitrate. The pH values of the incubation mixtures were 7.2 ± 0.07 and 7.1 ± 0.07 in the presence of nitrite and nitrate, respectively. The N-Nitrosodimethylamine (NDMA) level in cell debris was significantly higher compared with cell extract, cell suspension and sterile controls. The time-course of NDMA formation in cell debris showed a significant (p m) value was 37.5% lower, while initial velocity (Vmax) was 20.0% higher in cell debris relative to cell extract, when a fixed DMA concentration was used against varying nitrite concentrations. However, at a fixed nitrite concentration against varying DMA concentration, Km value was 44.4% lower, while Vmax was 166.7% higher in cell debris compared with cell extract. Investigation of nitrosation mechanism showed that Vmax values in the cell debris were 41.7, 50.0 and 55.6 μmol NDMA/mg protein, while the values were 34.5, 37.0 and 43.5 μmol NDMA/mg protein in cell extract for the respective three nitrite concentrations. This study has shown that intact Bacillus cereus cells catalyzed nitrosation of dimethylamine at near neutral pH, and the nitrosation, which was higher in cell debris than cell extract of the bacterium, followed a sequential mechanism of enzyme catalysis.},
 year = {2018}
}

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