Strong Radical Scavenging Macrofungi from the Dry Zone Forest Reserves in Sri Lanka

Dilusha Fernando; Ravi Wijesundera; Preethi Soysa; Dilip de Silva; Chandrika Nanayakkara

doi:doi:10.11648/j.fem.20150102.15

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Strong Radical Scavenging Macrofungi from the Dry Zone Forest Reserves in Sri Lanka

Dilusha Fernando, Ravi Wijesundera, Preethi Soysa, Dilip de Silva, Chandrika Nanayakkara

Published in Frontiers in Environmental Microbiology (Volume 1, Issue 2)

Received: 1 September 2015 Accepted: 10 September 2015 Published: 14 September 2015

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Abstract

Natural metabolites produced by macro fungi are of great interest as potential antioxidant defensive agents to reduce the oxidative damage caused by free radicals. Primarily, phenolic and flavonoid type metabolites have gained major importance due to the strong capacity of scavenging free radicals. The study was mainly focused to investigate the natural antioxidant properties of macro fungi found in Sri Lankan dry zone forest reserves using DPPH radical scavenging assay and to find out the contribution of phenol and flavonoid substances towards their antioxidant capacity. EC50 values of all extracts were below 1.2 mg/ml. Among the analyzed specimens, Phellinus repandus and Inonotus porrectus showed the most potent antioxidant activities having EC₅₀ of 7.91 ± 1.38 µg/ ml and 19.70 ± 0.17 µg/ ml, respectively. Ten fungal forms exhibited EC₅₀ < 300 µg/ ml and eighteen showed a mean values of EC₅₀ in the range of 300-1200 µg/ ml. Further, P. repandus and I. porrectus also exhibited the highest level of total phenols and flavonoids. EC₅₀ values of the species studied were inversely related to the total phenol and flavonoid contents. The analyzed macrofungi specimens exhibited high antioxidant power highlighting their potential as therapeutically useful antioxidant agents. Particularly, P. repandus and I. porrectus could be an important source of novel antioxidant compounds. In addition, phenol and flavonoid compounds largely contribute to the scavenging activity of studied macrofungi.

Published in	Frontiers in Environmental Microbiology (Volume 1, Issue 2)
DOI	10.11648/j.fem.20150102.15
Page(s)	32-38
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2015. Published by Science Publishing Group

Keywords

Macrofungi, Antioxidant Activity, Phenol Content, Flavonoid Content, EC₅₀

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APA Style

Dilusha Fernando, Ravi Wijesundera, Preethi Soysa, Dilip de Silva, Chandrika Nanayakkara. (2015). Strong Radical Scavenging Macrofungi from the Dry Zone Forest Reserves in Sri Lanka. Frontiers in Environmental Microbiology, 1(2), 32-38. https://doi.org/10.11648/j.fem.20150102.15

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ACS Style

Dilusha Fernando; Ravi Wijesundera; Preethi Soysa; Dilip de Silva; Chandrika Nanayakkara. Strong Radical Scavenging Macrofungi from the Dry Zone Forest Reserves in Sri Lanka. Front. Environ. Microbiol. 2015, 1(2), 32-38. doi: 10.11648/j.fem.20150102.15

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AMA Style

Dilusha Fernando, Ravi Wijesundera, Preethi Soysa, Dilip de Silva, Chandrika Nanayakkara. Strong Radical Scavenging Macrofungi from the Dry Zone Forest Reserves in Sri Lanka. Front Environ Microbiol. 2015;1(2):32-38. doi: 10.11648/j.fem.20150102.15

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@article{10.11648/j.fem.20150102.15,
  author = {Dilusha Fernando and Ravi Wijesundera and Preethi Soysa and Dilip de Silva and Chandrika Nanayakkara},
  title = {Strong Radical Scavenging Macrofungi from the Dry Zone Forest Reserves in Sri Lanka},
  journal = {Frontiers in Environmental Microbiology},
  volume = {1},
  number = {2},
  pages = {32-38},
  doi = {10.11648/j.fem.20150102.15},
  url = {https://doi.org/10.11648/j.fem.20150102.15},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.fem.20150102.15},
  abstract = {Natural metabolites produced by macro fungi are of great interest as potential antioxidant defensive agents to reduce the oxidative damage caused by free radicals. Primarily, phenolic and flavonoid type metabolites have gained major importance due to the strong capacity of scavenging free radicals. The study was mainly focused to investigate the natural antioxidant properties of macro fungi found in Sri Lankan dry zone forest reserves using DPPH radical scavenging assay and to find out the contribution of phenol and flavonoid substances towards their antioxidant capacity. EC50 values of all extracts were below 1.2 mg/ml. Among the analyzed specimens, Phellinus repandus and Inonotus porrectus showed the most potent antioxidant activities having EC50 of 7.91 ± 1.38 µg/ ml and 19.70 ± 0.17 µg/ ml, respectively. Ten fungal forms exhibited EC50 50 in the range of 300-1200 µg/ ml. Further, P. repandus and I. porrectus also exhibited the highest level of total phenols and flavonoids. EC50 values of the species studied were inversely related to the total phenol and flavonoid contents. The analyzed macrofungi specimens exhibited high antioxidant power highlighting their potential as therapeutically useful antioxidant agents. Particularly, P. repandus and I. porrectus could be an important source of novel antioxidant compounds. In addition, phenol and flavonoid compounds largely contribute to the scavenging activity of studied macrofungi.},
 year = {2015}
}

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TY - JOUR
T1 - Strong Radical Scavenging Macrofungi from the Dry Zone Forest Reserves in Sri Lanka
AU - Dilusha Fernando
AU - Ravi Wijesundera
AU - Preethi Soysa
AU - Dilip de Silva
AU - Chandrika Nanayakkara
Y1 - 2015/09/14
PY - 2015
N1 - https://doi.org/10.11648/j.fem.20150102.15
DO - 10.11648/j.fem.20150102.15
T2 - Frontiers in Environmental Microbiology
JF - Frontiers in Environmental Microbiology
JO - Frontiers in Environmental Microbiology
SP - 32
EP - 38
PB - Science Publishing Group
SN - 2469-8067
UR - https://doi.org/10.11648/j.fem.20150102.15
AB - Natural metabolites produced by macro fungi are of great interest as potential antioxidant defensive agents to reduce the oxidative damage caused by free radicals. Primarily, phenolic and flavonoid type metabolites have gained major importance due to the strong capacity of scavenging free radicals. The study was mainly focused to investigate the natural antioxidant properties of macro fungi found in Sri Lankan dry zone forest reserves using DPPH radical scavenging assay and to find out the contribution of phenol and flavonoid substances towards their antioxidant capacity. EC50 values of all extracts were below 1.2 mg/ml. Among the analyzed specimens, Phellinus repandus and Inonotus porrectus showed the most potent antioxidant activities having EC50 of 7.91 ± 1.38 µg/ ml and 19.70 ± 0.17 µg/ ml, respectively. Ten fungal forms exhibited EC50 50 in the range of 300-1200 µg/ ml. Further, P. repandus and I. porrectus also exhibited the highest level of total phenols and flavonoids. EC50 values of the species studied were inversely related to the total phenol and flavonoid contents. The analyzed macrofungi specimens exhibited high antioxidant power highlighting their potential as therapeutically useful antioxidant agents. Particularly, P. repandus and I. porrectus could be an important source of novel antioxidant compounds. In addition, phenol and flavonoid compounds largely contribute to the scavenging activity of studied macrofungi.
VL - 1
IS - 2
ER -

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Author Information

Dilusha Fernando

Department of Plant Sciences, Faculty of Science, University of Colombo, Colombo, Sri Lanka
Ravi Wijesundera

Department of Plant Sciences, Faculty of Science, University of Colombo, Colombo, Sri Lanka
Preethi Soysa

Department of Molecular Biology and Biochemistry, Faculty of Medicine, University of Colombo, Colombo, Sri Lanka
Dilip de Silva

Department of Chemistry, Faculty of Science, University of Colombo, Colombo, Sri Lanka
Chandrika Nanayakkara

Department of Plant Sciences, Faculty of Science, University of Colombo, Colombo, Sri Lanka

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APA Style

Dilusha Fernando, Ravi Wijesundera, Preethi Soysa, Dilip de Silva, Chandrika Nanayakkara. (2015). Strong Radical Scavenging Macrofungi from the Dry Zone Forest Reserves in Sri Lanka. Frontiers in Environmental Microbiology, 1(2), 32-38. https://doi.org/10.11648/j.fem.20150102.15

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ACS Style

Dilusha Fernando; Ravi Wijesundera; Preethi Soysa; Dilip de Silva; Chandrika Nanayakkara. Strong Radical Scavenging Macrofungi from the Dry Zone Forest Reserves in Sri Lanka. Front. Environ. Microbiol. 2015, 1(2), 32-38. doi: 10.11648/j.fem.20150102.15

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AMA Style

Dilusha Fernando, Ravi Wijesundera, Preethi Soysa, Dilip de Silva, Chandrika Nanayakkara. Strong Radical Scavenging Macrofungi from the Dry Zone Forest Reserves in Sri Lanka. Front Environ Microbiol. 2015;1(2):32-38. doi: 10.11648/j.fem.20150102.15

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@article{10.11648/j.fem.20150102.15,
  author = {Dilusha Fernando and Ravi Wijesundera and Preethi Soysa and Dilip de Silva and Chandrika Nanayakkara},
  title = {Strong Radical Scavenging Macrofungi from the Dry Zone Forest Reserves in Sri Lanka},
  journal = {Frontiers in Environmental Microbiology},
  volume = {1},
  number = {2},
  pages = {32-38},
  doi = {10.11648/j.fem.20150102.15},
  url = {https://doi.org/10.11648/j.fem.20150102.15},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.fem.20150102.15},
  abstract = {Natural metabolites produced by macro fungi are of great interest as potential antioxidant defensive agents to reduce the oxidative damage caused by free radicals. Primarily, phenolic and flavonoid type metabolites have gained major importance due to the strong capacity of scavenging free radicals. The study was mainly focused to investigate the natural antioxidant properties of macro fungi found in Sri Lankan dry zone forest reserves using DPPH radical scavenging assay and to find out the contribution of phenol and flavonoid substances towards their antioxidant capacity. EC50 values of all extracts were below 1.2 mg/ml. Among the analyzed specimens, Phellinus repandus and Inonotus porrectus showed the most potent antioxidant activities having EC50 of 7.91 ± 1.38 µg/ ml and 19.70 ± 0.17 µg/ ml, respectively. Ten fungal forms exhibited EC50 50 in the range of 300-1200 µg/ ml. Further, P. repandus and I. porrectus also exhibited the highest level of total phenols and flavonoids. EC50 values of the species studied were inversely related to the total phenol and flavonoid contents. The analyzed macrofungi specimens exhibited high antioxidant power highlighting their potential as therapeutically useful antioxidant agents. Particularly, P. repandus and I. porrectus could be an important source of novel antioxidant compounds. In addition, phenol and flavonoid compounds largely contribute to the scavenging activity of studied macrofungi.},
 year = {2015}
}

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