Understanding of diffusion of solute particles across the biological partition is very well studied both in vitro and in vivo. Diffusion is the movement of solute molecules by random thermal or Brownian motion across the barriers. Various experimental and mathematical models were employed to study the diffusion characteristics which have also helped to design the experiments and interpretation of data. In the present investigation, we have developed a simple, cost-effective eggshell membrane model for understanding diffusion characteristics. The passive diffusion (in vitro) of sugar molecules through a natural (Chicken egg shell) membrane model was studied. The diffusion coefficient (D), diffusion rate (J), and permeability coefficient (P) in relation to temperature, viscosity of solution, surface area of the membrane, and the molecular size of diffusing molecules have been quantitatively determined. Fick’s first law of diffusion is used for the evaluation of experimental data. All the experiments were carried out at a physiological pH. The diffused solute particles in the donor compartment were measured using UV visible spectroscopy at three different time intervals. Diffusion rate and the permeability coefficient were found to increase with the higher temperature of the solution as well as for the larger surface area of the membrane; however, it decreased with the viscosity and size of the diffusing solute particles. The possible mechanisms and characteristics of the diffusion of the molecules have been elucidated. We conclude that such a model may be useful as a teaching and learning model for diffusion characteristics.
| Published in | International Journal of Biochemistry, Biophysics & Molecular Biology (Volume 8, Issue 1) |
| DOI | 10.11648/j.ijbbmb.20230801.12 |
| Page(s) | 6-11 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2023. Published by Science Publishing Group |
Diffusion, Eggshell Membrane, Sugars, Surface Area, Temperature, Viscosity
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APA Style
Shah, P. K. K., Jaiswal, V. D., Kulkarni, A. A., Dongre, P. M. (2023). Simple and Cost-Effective Eggshell Membrane Model for Diffusion Characteristics of Biochemical Materials. International Journal of Biochemistry, Biophysics & Molecular Biology, 8(1), 6-11. https://doi.org/10.11648/j.ijbbmb.20230801.12
ACS Style
Shah, P. K. K.; Jaiswal, V. D.; Kulkarni, A. A.; Dongre, P. M. Simple and Cost-Effective Eggshell Membrane Model for Diffusion Characteristics of Biochemical Materials. Int. J. Biochem. Biophys. Mol. Biol. 2023, 8(1), 6-11. doi: 10.11648/j.ijbbmb.20230801.12
AMA Style
Shah PKK, Jaiswal VD, Kulkarni AA, Dongre PM. Simple and Cost-Effective Eggshell Membrane Model for Diffusion Characteristics of Biochemical Materials. Int J Biochem Biophys Mol Biol. 2023;8(1):6-11. doi: 10.11648/j.ijbbmb.20230801.12
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Download@article{10.11648/j.ijbbmb.20230801.12,
author = {Puja Kirti Kumar Shah and Vinod Deomani Jaiswal and Amol Anant Kulkarni and Prabhakar Manikrao Dongre},
title = {Simple and Cost-Effective Eggshell Membrane Model for Diffusion Characteristics of Biochemical Materials},
journal = {International Journal of Biochemistry, Biophysics & Molecular Biology},
volume = {8},
number = {1},
pages = {6-11},
doi = {10.11648/j.ijbbmb.20230801.12},
url = {https://doi.org/10.11648/j.ijbbmb.20230801.12},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijbbmb.20230801.12},
abstract = {Understanding of diffusion of solute particles across the biological partition is very well studied both in vitro and in vivo. Diffusion is the movement of solute molecules by random thermal or Brownian motion across the barriers. Various experimental and mathematical models were employed to study the diffusion characteristics which have also helped to design the experiments and interpretation of data. In the present investigation, we have developed a simple, cost-effective eggshell membrane model for understanding diffusion characteristics. The passive diffusion (in vitro) of sugar molecules through a natural (Chicken egg shell) membrane model was studied. The diffusion coefficient (D), diffusion rate (J), and permeability coefficient (P) in relation to temperature, viscosity of solution, surface area of the membrane, and the molecular size of diffusing molecules have been quantitatively determined. Fick’s first law of diffusion is used for the evaluation of experimental data. All the experiments were carried out at a physiological pH. The diffused solute particles in the donor compartment were measured using UV visible spectroscopy at three different time intervals. Diffusion rate and the permeability coefficient were found to increase with the higher temperature of the solution as well as for the larger surface area of the membrane; however, it decreased with the viscosity and size of the diffusing solute particles. The possible mechanisms and characteristics of the diffusion of the molecules have been elucidated. We conclude that such a model may be useful as a teaching and learning model for diffusion characteristics.
},
year = {2023}
}
TY - JOUR T1 - Simple and Cost-Effective Eggshell Membrane Model for Diffusion Characteristics of Biochemical Materials AU - Puja Kirti Kumar Shah AU - Vinod Deomani Jaiswal AU - Amol Anant Kulkarni AU - Prabhakar Manikrao Dongre Y1 - 2023/11/09 PY - 2023 N1 - https://doi.org/10.11648/j.ijbbmb.20230801.12 DO - 10.11648/j.ijbbmb.20230801.12 T2 - International Journal of Biochemistry, Biophysics & Molecular Biology JF - International Journal of Biochemistry, Biophysics & Molecular Biology JO - International Journal of Biochemistry, Biophysics & Molecular Biology SP - 6 EP - 11 PB - Science Publishing Group SN - 2575-5862 UR - https://doi.org/10.11648/j.ijbbmb.20230801.12 AB - Understanding of diffusion of solute particles across the biological partition is very well studied both in vitro and in vivo. Diffusion is the movement of solute molecules by random thermal or Brownian motion across the barriers. Various experimental and mathematical models were employed to study the diffusion characteristics which have also helped to design the experiments and interpretation of data. In the present investigation, we have developed a simple, cost-effective eggshell membrane model for understanding diffusion characteristics. The passive diffusion (in vitro) of sugar molecules through a natural (Chicken egg shell) membrane model was studied. The diffusion coefficient (D), diffusion rate (J), and permeability coefficient (P) in relation to temperature, viscosity of solution, surface area of the membrane, and the molecular size of diffusing molecules have been quantitatively determined. Fick’s first law of diffusion is used for the evaluation of experimental data. All the experiments were carried out at a physiological pH. The diffused solute particles in the donor compartment were measured using UV visible spectroscopy at three different time intervals. Diffusion rate and the permeability coefficient were found to increase with the higher temperature of the solution as well as for the larger surface area of the membrane; however, it decreased with the viscosity and size of the diffusing solute particles. The possible mechanisms and characteristics of the diffusion of the molecules have been elucidated. We conclude that such a model may be useful as a teaching and learning model for diffusion characteristics. VL - 8 IS - 1 ER -
Department of Biophysics, University of Mumbai, Mumbai, India
Department of Biophysics, University of Mumbai, Mumbai, India
Vasantdada Patil Dental College and Hospital, Kavalpur, Sangli, India
Department of Biophysics, University of Mumbai, Mumbai, India; Pravara Rural Education Society’s ACS College Satral, Ahmednagar, India
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