Entamoeba histolytica is ranked third on the list of parasitic protozoan infections leading to death behind malaria and schistosomiasis. It is estimated also up to 200 millions of people are chronically infected with Giardia lamblia globally. Metronidazole is the drug currently widely used and recommended in the treatment of amoebiasis and giardiasis. However; sometimes it causes adverse effects such as myoplasia, neuralgia, allergic dermatitis, and others. The in vitro antiprotozoal activities of some selected Sudanese medicinal plants (Acacia nilotica subsp. nilotica, Adansonia digitata, Cyperus rotundus and Nigella sativa) were determined against Entamoeba histolytica and Giardia lamblia by employing the sub-culture method. The mammalian cytotoxicity of the investigated plants against normal Vero cell line was determined by applying MTT [(4, 5-dimethyl thiazol-2-yl)-2, 5-diphenyl tetrazolium bromide] method. All plants examined 100% inhibition at a concentration 500 μg/ml after 96 h; this was compared with Metronidazole which gave 95% inhibition at concentration 312.5 μg/ml at the same time against Entamoeba histolytica and Giardia lamblia. In addition, cytotoxicity (MTT-assay) of these plants against normal Vero cell line which verified the safety of the examined extracts with an IC50 less 100 μg/ml. These studies prove the potent activity of extracts against E. histolyica and G. lamblia trophozoites in vitro with verified safety evidence for use.
| Published in | International Journal of Biomedical Engineering and Clinical Science (Volume 3, Issue 2) |
| DOI | 10.11648/j.ijbecs.20170302.11 |
| Page(s) | 6-13 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2017. Published by Science Publishing Group |
Entamoeba histolytica, Giardia lamblia, Medicinal Plants, Metronidazole, Cytotoxicity
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APA Style
Ahmed S. Kabbashi, El-badri E. Osman, Mohamed I. Garbi, Ibrahim F. Ahmed, Mahmmoud S. Saleh, et al. (2017). In Vitro Antiprotozoal Activities and Cytotoxicity of Selected Sudanese Medicinal Plants. International Journal of Biomedical Engineering and Clinical Science, 3(2), 6-13. https://doi.org/10.11648/j.ijbecs.20170302.11
ACS Style
Ahmed S. Kabbashi; El-badri E. Osman; Mohamed I. Garbi; Ibrahim F. Ahmed; Mahmmoud S. Saleh, et al. In Vitro Antiprotozoal Activities and Cytotoxicity of Selected Sudanese Medicinal Plants. Int. J. Biomed. Eng. Clin. Sci. 2017, 3(2), 6-13. doi: 10.11648/j.ijbecs.20170302.11
AMA Style
Ahmed S. Kabbashi, El-badri E. Osman, Mohamed I. Garbi, Ibrahim F. Ahmed, Mahmmoud S. Saleh, et al. In Vitro Antiprotozoal Activities and Cytotoxicity of Selected Sudanese Medicinal Plants. Int J Biomed Eng Clin Sci. 2017;3(2):6-13. doi: 10.11648/j.ijbecs.20170302.11
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Download@article{10.11648/j.ijbecs.20170302.11,
author = {Ahmed S. Kabbashi and El-badri E. Osman and Mohamed I. Garbi and Ibrahim F. Ahmed and Mahmmoud S. Saleh and Ali M. Badri and Ahmed A. Elshikh and Nadir Abuzeid and Waleed S. Koko and Mahmoud M. Dahab},
title = {In Vitro Antiprotozoal Activities and Cytotoxicity of Selected Sudanese Medicinal Plants},
journal = {International Journal of Biomedical Engineering and Clinical Science},
volume = {3},
number = {2},
pages = {6-13},
doi = {10.11648/j.ijbecs.20170302.11},
url = {https://doi.org/10.11648/j.ijbecs.20170302.11},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijbecs.20170302.11},
abstract = {Entamoeba histolytica is ranked third on the list of parasitic protozoan infections leading to death behind malaria and schistosomiasis. It is estimated also up to 200 millions of people are chronically infected with Giardia lamblia globally. Metronidazole is the drug currently widely used and recommended in the treatment of amoebiasis and giardiasis. However; sometimes it causes adverse effects such as myoplasia, neuralgia, allergic dermatitis, and others. The in vitro antiprotozoal activities of some selected Sudanese medicinal plants (Acacia nilotica subsp. nilotica, Adansonia digitata, Cyperus rotundus and Nigella sativa) were determined against Entamoeba histolytica and Giardia lamblia by employing the sub-culture method. The mammalian cytotoxicity of the investigated plants against normal Vero cell line was determined by applying MTT [(4, 5-dimethyl thiazol-2-yl)-2, 5-diphenyl tetrazolium bromide] method. All plants examined 100% inhibition at a concentration 500 μg/ml after 96 h; this was compared with Metronidazole which gave 95% inhibition at concentration 312.5 μg/ml at the same time against Entamoeba histolytica and Giardia lamblia. In addition, cytotoxicity (MTT-assay) of these plants against normal Vero cell line which verified the safety of the examined extracts with an IC50 less 100 μg/ml. These studies prove the potent activity of extracts against E. histolyica and G. lamblia trophozoites in vitro with verified safety evidence for use.},
year = {2017}
}
TY - JOUR T1 - In Vitro Antiprotozoal Activities and Cytotoxicity of Selected Sudanese Medicinal Plants AU - Ahmed S. Kabbashi AU - El-badri E. Osman AU - Mohamed I. Garbi AU - Ibrahim F. Ahmed AU - Mahmmoud S. Saleh AU - Ali M. Badri AU - Ahmed A. Elshikh AU - Nadir Abuzeid AU - Waleed S. Koko AU - Mahmoud M. Dahab Y1 - 2017/08/23 PY - 2017 N1 - https://doi.org/10.11648/j.ijbecs.20170302.11 DO - 10.11648/j.ijbecs.20170302.11 T2 - International Journal of Biomedical Engineering and Clinical Science JF - International Journal of Biomedical Engineering and Clinical Science JO - International Journal of Biomedical Engineering and Clinical Science SP - 6 EP - 13 PB - Science Publishing Group SN - 2472-1301 UR - https://doi.org/10.11648/j.ijbecs.20170302.11 AB - Entamoeba histolytica is ranked third on the list of parasitic protozoan infections leading to death behind malaria and schistosomiasis. It is estimated also up to 200 millions of people are chronically infected with Giardia lamblia globally. Metronidazole is the drug currently widely used and recommended in the treatment of amoebiasis and giardiasis. However; sometimes it causes adverse effects such as myoplasia, neuralgia, allergic dermatitis, and others. The in vitro antiprotozoal activities of some selected Sudanese medicinal plants (Acacia nilotica subsp. nilotica, Adansonia digitata, Cyperus rotundus and Nigella sativa) were determined against Entamoeba histolytica and Giardia lamblia by employing the sub-culture method. The mammalian cytotoxicity of the investigated plants against normal Vero cell line was determined by applying MTT [(4, 5-dimethyl thiazol-2-yl)-2, 5-diphenyl tetrazolium bromide] method. All plants examined 100% inhibition at a concentration 500 μg/ml after 96 h; this was compared with Metronidazole which gave 95% inhibition at concentration 312.5 μg/ml at the same time against Entamoeba histolytica and Giardia lamblia. In addition, cytotoxicity (MTT-assay) of these plants against normal Vero cell line which verified the safety of the examined extracts with an IC50 less 100 μg/ml. These studies prove the potent activity of extracts against E. histolyica and G. lamblia trophozoites in vitro with verified safety evidence for use. VL - 3 IS - 2 ER -
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