Preparation of Low Cost Affinity Chromatographic Matrix and Its Application in Purification of a Lectin Isolated from Mulberry Seeds

Mosammat Jesmin Sultana; Mohammad Taufiq Alam; Fazle Rabbi Shakil Ahmed

doi:doi:10.11648/j.ijbmr.20180602.14

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Preparation of Low Cost Affinity Chromatographic Matrix and Its Application in Purification of a Lectin Isolated from Mulberry Seeds

Mosammat Jesmin Sultana, Mohammad Taufiq Alam, Fazle Rabbi Shakil Ahmed

Published in International Journal of Biomedical Materials Research (Volume 6, Issue 2)

Received: 22 June 2018 Accepted: 17 July 2018 Published: 30 August 2018

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Abstract

Objective: Uniform sized agarose gel beads were prepared from agarose by emulsification technique. The prepared matrix has been attached with commercially available concanavalin A (conA) and finally it has applied to purify mulberry seed lectin. The present work shows a simple and inexpensive method for the preparation of an affinity matrix for purification of conA specific mulberry seed lectin. Method: Firstly, conA was immobilized on agarose gel beads and coupled to hexadiamine by using amino reactive bifunctional crosslinker (2,4,6-trichloro-1,3,5-trizine). Then it used as affinity matrix for the purification of mulberry seed lectin (designated as MSL). In purification protocol initially conA has been immobilized with agarose. Then MSL have been purified from the seeds of Morus alba L. Result: The agarose beads showed the best uniformity when 2-2.5% concentration of Tween 80 was used. The newly prepared affinity matrix agarose gel was able to purify MSL with the molecular weight of 22 kDa in a single step. The purified lectin strongly agglutinated with mice, chicken, bovine and human blood types A, B and O erythrocytes respectively. There was no activity found on goat erythrocytes. Conclusion: The present work shows a simple and inexpensive method for the preparation of an affinity chromatographic matrix. The prepared matrix was applied to purify mulberry seed lectin.

Published in	International Journal of Biomedical Materials Research (Volume 6, Issue 2)
DOI	10.11648/j.ijbmr.20180602.14
Page(s)	50-56
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2018. Published by Science Publishing Group

Keywords

Immobilization, Affinity Matrix, Affinity Chromatography, Epichlorohydrin, Lectin, Hemagglutination

References

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APA Style

Mosammat Jesmin Sultana, Mohammad Taufiq Alam, Fazle Rabbi Shakil Ahmed. (2018). Preparation of Low Cost Affinity Chromatographic Matrix and Its Application in Purification of a Lectin Isolated from Mulberry Seeds. International Journal of Biomedical Materials Research, 6(2), 50-56. https://doi.org/10.11648/j.ijbmr.20180602.14

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ACS Style

Mosammat Jesmin Sultana; Mohammad Taufiq Alam; Fazle Rabbi Shakil Ahmed. Preparation of Low Cost Affinity Chromatographic Matrix and Its Application in Purification of a Lectin Isolated from Mulberry Seeds. Int. J. Biomed. Mater. Res. 2018, 6(2), 50-56. doi: 10.11648/j.ijbmr.20180602.14

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AMA Style

Mosammat Jesmin Sultana, Mohammad Taufiq Alam, Fazle Rabbi Shakil Ahmed. Preparation of Low Cost Affinity Chromatographic Matrix and Its Application in Purification of a Lectin Isolated from Mulberry Seeds. Int J Biomed Mater Res. 2018;6(2):50-56. doi: 10.11648/j.ijbmr.20180602.14

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@article{10.11648/j.ijbmr.20180602.14,
  author = {Mosammat Jesmin Sultana and Mohammad Taufiq Alam and Fazle Rabbi Shakil Ahmed},
  title = {Preparation of Low Cost Affinity Chromatographic Matrix and Its Application in Purification of a Lectin Isolated from Mulberry Seeds},
  journal = {International Journal of Biomedical Materials Research},
  volume = {6},
  number = {2},
  pages = {50-56},
  doi = {10.11648/j.ijbmr.20180602.14},
  url = {https://doi.org/10.11648/j.ijbmr.20180602.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijbmr.20180602.14},
  abstract = {Objective: Uniform sized agarose gel beads were prepared from agarose by emulsification technique. The prepared matrix has been attached with commercially available concanavalin A (conA) and finally it has applied to purify mulberry seed lectin. The present work shows a simple and inexpensive method for the preparation of an affinity matrix for purification of conA specific mulberry seed lectin. Method: Firstly, conA was immobilized on agarose gel beads and coupled to hexadiamine by using amino reactive bifunctional crosslinker (2,4,6-trichloro-1,3,5-trizine). Then it used as affinity matrix for the purification of mulberry seed lectin (designated as MSL). In purification protocol initially conA has been immobilized with agarose. Then MSL have been purified from the seeds of Morus alba L. Result: The agarose beads showed the best uniformity when 2-2.5% concentration of Tween 80 was used. The newly prepared affinity matrix agarose gel was able to purify MSL with the molecular weight of 22 kDa in a single step. The purified lectin strongly agglutinated with mice, chicken, bovine and human blood types A, B and O erythrocytes respectively. There was no activity found on goat erythrocytes. Conclusion: The present work shows a simple and inexpensive method for the preparation of an affinity chromatographic matrix. The prepared matrix was applied to purify mulberry seed lectin.},
 year = {2018}
}

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TY - JOUR
T1 - Preparation of Low Cost Affinity Chromatographic Matrix and Its Application in Purification of a Lectin Isolated from Mulberry Seeds
AU - Mosammat Jesmin Sultana
AU - Mohammad Taufiq Alam
AU - Fazle Rabbi Shakil Ahmed
Y1 - 2018/08/30
PY - 2018
N1 - https://doi.org/10.11648/j.ijbmr.20180602.14
DO - 10.11648/j.ijbmr.20180602.14
T2 - International Journal of Biomedical Materials Research
JF - International Journal of Biomedical Materials Research
JO - International Journal of Biomedical Materials Research
SP - 50
EP - 56
PB - Science Publishing Group
SN - 2330-7579
UR - https://doi.org/10.11648/j.ijbmr.20180602.14
AB - Objective: Uniform sized agarose gel beads were prepared from agarose by emulsification technique. The prepared matrix has been attached with commercially available concanavalin A (conA) and finally it has applied to purify mulberry seed lectin. The present work shows a simple and inexpensive method for the preparation of an affinity matrix for purification of conA specific mulberry seed lectin. Method: Firstly, conA was immobilized on agarose gel beads and coupled to hexadiamine by using amino reactive bifunctional crosslinker (2,4,6-trichloro-1,3,5-trizine). Then it used as affinity matrix for the purification of mulberry seed lectin (designated as MSL). In purification protocol initially conA has been immobilized with agarose. Then MSL have been purified from the seeds of Morus alba L. Result: The agarose beads showed the best uniformity when 2-2.5% concentration of Tween 80 was used. The newly prepared affinity matrix agarose gel was able to purify MSL with the molecular weight of 22 kDa in a single step. The purified lectin strongly agglutinated with mice, chicken, bovine and human blood types A, B and O erythrocytes respectively. There was no activity found on goat erythrocytes. Conclusion: The present work shows a simple and inexpensive method for the preparation of an affinity chromatographic matrix. The prepared matrix was applied to purify mulberry seed lectin.
VL - 6
IS - 2
ER -

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Author Information

Mosammat Jesmin Sultana

Department of Materials Science and Engineering, University of Rajshahi, Rajshahi, Bangladesh
Mohammad Taufiq Alam

Department of Applied Chemistry and Chemical Engineering, University of Rajshahi, Rajshahi, Bangladesh
Fazle Rabbi Shakil Ahmed

Department of Pharmacy, Khwaja Yunus Ali University, Sirajgonj, Bangladesh

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APA Style

Mosammat Jesmin Sultana, Mohammad Taufiq Alam, Fazle Rabbi Shakil Ahmed. (2018). Preparation of Low Cost Affinity Chromatographic Matrix and Its Application in Purification of a Lectin Isolated from Mulberry Seeds. International Journal of Biomedical Materials Research, 6(2), 50-56. https://doi.org/10.11648/j.ijbmr.20180602.14

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ACS Style

Mosammat Jesmin Sultana; Mohammad Taufiq Alam; Fazle Rabbi Shakil Ahmed. Preparation of Low Cost Affinity Chromatographic Matrix and Its Application in Purification of a Lectin Isolated from Mulberry Seeds. Int. J. Biomed. Mater. Res. 2018, 6(2), 50-56. doi: 10.11648/j.ijbmr.20180602.14

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AMA Style

Mosammat Jesmin Sultana, Mohammad Taufiq Alam, Fazle Rabbi Shakil Ahmed. Preparation of Low Cost Affinity Chromatographic Matrix and Its Application in Purification of a Lectin Isolated from Mulberry Seeds. Int J Biomed Mater Res. 2018;6(2):50-56. doi: 10.11648/j.ijbmr.20180602.14

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@article{10.11648/j.ijbmr.20180602.14,
  author = {Mosammat Jesmin Sultana and Mohammad Taufiq Alam and Fazle Rabbi Shakil Ahmed},
  title = {Preparation of Low Cost Affinity Chromatographic Matrix and Its Application in Purification of a Lectin Isolated from Mulberry Seeds},
  journal = {International Journal of Biomedical Materials Research},
  volume = {6},
  number = {2},
  pages = {50-56},
  doi = {10.11648/j.ijbmr.20180602.14},
  url = {https://doi.org/10.11648/j.ijbmr.20180602.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijbmr.20180602.14},
  abstract = {Objective: Uniform sized agarose gel beads were prepared from agarose by emulsification technique. The prepared matrix has been attached with commercially available concanavalin A (conA) and finally it has applied to purify mulberry seed lectin. The present work shows a simple and inexpensive method for the preparation of an affinity matrix for purification of conA specific mulberry seed lectin. Method: Firstly, conA was immobilized on agarose gel beads and coupled to hexadiamine by using amino reactive bifunctional crosslinker (2,4,6-trichloro-1,3,5-trizine). Then it used as affinity matrix for the purification of mulberry seed lectin (designated as MSL). In purification protocol initially conA has been immobilized with agarose. Then MSL have been purified from the seeds of Morus alba L. Result: The agarose beads showed the best uniformity when 2-2.5% concentration of Tween 80 was used. The newly prepared affinity matrix agarose gel was able to purify MSL with the molecular weight of 22 kDa in a single step. The purified lectin strongly agglutinated with mice, chicken, bovine and human blood types A, B and O erythrocytes respectively. There was no activity found on goat erythrocytes. Conclusion: The present work shows a simple and inexpensive method for the preparation of an affinity chromatographic matrix. The prepared matrix was applied to purify mulberry seed lectin.},
 year = {2018}
}

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