Determination of the Concentration of Polyamines with SPR-Based Immune Biosensor for Early Diagnostics of Breast Cancer

Maksym Prylutskyi; Nickolaj Starodub; Nadiia Bilko

doi:doi:10.11648/j.ijcocr.20160101.15

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Determination of the Concentration of Polyamines with SPR-Based Immune Biosensor for Early Diagnostics of Breast Cancer

Maksym Prylutskyi, Nickolaj Starodub, Nadiia Bilko

Published in International Journal of Clinical Oncology and Cancer Research (Volume 1, Issue 1)

Received: 13 November 2016 Accepted: 3 December 2016 Published: 17 January 2017

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Abstract

The paper presents the results of research on the development of immune biosensor test system for express detection of polyamines in cells of breast cancer. Determination of polyamines was performed by using an analytical device - immune biosensor based on surface plasmon resonance (SPR), where "antigen-antibody" reaction is performed in real time on the surface of transducer, resulting in formation of immune complexes and recording the shift of resonance angle. It was performed two methods of analysis: direct, when immobilized specific antibodies interacted with appropriate antigens and competitive, when the one part of antigens was conjugated with protein and competed for binding with specific antibodies with free antigens. In relation to direct analysis it was shown the dependence of sensitivity of biosensor response from the concentration of polyamines in range of 10-100 ng /ml. Concerning the competitive method of analysis, the linear region of sensitivity was in range of 0,3-80 ng/ml and 0,01-90 ng/ml for spermine and spermidine respectively.

Published in	International Journal of Clinical Oncology and Cancer Research (Volume 1, Issue 1)
DOI	10.11648/j.ijcocr.20160101.15
Page(s)	30-35
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2017. Published by Science Publishing Group

Keywords

SPR, Polyamines, Antibody, Antigen, Surface Plasmon Resonance, Breast Cancer

References

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APA Style

Maksym Prylutskyi, Nickolaj Starodub, Nadiia Bilko. (2017). Determination of the Concentration of Polyamines with SPR-Based Immune Biosensor for Early Diagnostics of Breast Cancer. International Journal of Clinical Oncology and Cancer Research, 1(1), 30-35. https://doi.org/10.11648/j.ijcocr.20160101.15

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ACS Style

Maksym Prylutskyi; Nickolaj Starodub; Nadiia Bilko. Determination of the Concentration of Polyamines with SPR-Based Immune Biosensor for Early Diagnostics of Breast Cancer. Int. J. Clin. Oncol. Cancer Res. 2017, 1(1), 30-35. doi: 10.11648/j.ijcocr.20160101.15

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AMA Style

Maksym Prylutskyi, Nickolaj Starodub, Nadiia Bilko. Determination of the Concentration of Polyamines with SPR-Based Immune Biosensor for Early Diagnostics of Breast Cancer. Int J Clin Oncol Cancer Res. 2017;1(1):30-35. doi: 10.11648/j.ijcocr.20160101.15

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@article{10.11648/j.ijcocr.20160101.15,
  author = {Maksym Prylutskyi and Nickolaj Starodub and Nadiia Bilko},
  title = {Determination of the Concentration of Polyamines with SPR-Based Immune Biosensor for Early Diagnostics of Breast Cancer},
  journal = {International Journal of Clinical Oncology and Cancer Research},
  volume = {1},
  number = {1},
  pages = {30-35},
  doi = {10.11648/j.ijcocr.20160101.15},
  url = {https://doi.org/10.11648/j.ijcocr.20160101.15},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijcocr.20160101.15},
  abstract = {The paper presents the results of research on the development of immune biosensor test system for express detection of polyamines in cells of breast cancer. Determination of polyamines was performed by using an analytical device - immune biosensor based on surface plasmon resonance (SPR), where "antigen-antibody" reaction is performed in real time on the surface of transducer, resulting in formation of immune complexes and recording the shift of resonance angle. It was performed two methods of analysis: direct, when immobilized specific antibodies interacted with appropriate antigens and competitive, when the one part of antigens was conjugated with protein and competed for binding with specific antibodies with free antigens. In relation to direct analysis it was shown the dependence of sensitivity of biosensor response from the concentration of polyamines in range of 10-100 ng /ml. Concerning the competitive method of analysis, the linear region of sensitivity was in range of 0,3-80 ng/ml and 0,01-90 ng/ml for spermine and spermidine respectively.},
 year = {2017}
}

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TY  - JOUR
T1  - Determination of the Concentration of Polyamines with SPR-Based Immune Biosensor for Early Diagnostics of Breast Cancer
AU  - Maksym Prylutskyi
AU  - Nickolaj Starodub
AU  - Nadiia Bilko
Y1  - 2017/01/17
PY  - 2017
N1  - https://doi.org/10.11648/j.ijcocr.20160101.15
DO  - 10.11648/j.ijcocr.20160101.15
T2  - International Journal of Clinical Oncology and Cancer Research
JF  - International Journal of Clinical Oncology and Cancer Research
JO  - International Journal of Clinical Oncology and Cancer Research
SP  - 30
EP  - 35
PB  - Science Publishing Group
SN  - 2578-9511
UR  - https://doi.org/10.11648/j.ijcocr.20160101.15
AB  - The paper presents the results of research on the development of immune biosensor test system for express detection of polyamines in cells of breast cancer. Determination of polyamines was performed by using an analytical device - immune biosensor based on surface plasmon resonance (SPR), where "antigen-antibody" reaction is performed in real time on the surface of transducer, resulting in formation of immune complexes and recording the shift of resonance angle. It was performed two methods of analysis: direct, when immobilized specific antibodies interacted with appropriate antigens and competitive, when the one part of antigens was conjugated with protein and competed for binding with specific antibodies with free antigens. In relation to direct analysis it was shown the dependence of sensitivity of biosensor response from the concentration of polyamines in range of 10-100 ng /ml. Concerning the competitive method of analysis, the linear region of sensitivity was in range of 0,3-80 ng/ml and 0,01-90 ng/ml for spermine and spermidine respectively.
VL  - 1
IS  - 1
ER  -

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Author Information

Maksym Prylutskyi

Department of Laboratory Diagnostics of Biological Systems, Center of Molecular and Cell Research of National University of Kyiv-Mohyla Academy, Kyiv, Ukraine
Nickolaj Starodub

Department of Molecular Biology, Microbiology and Biosafety of National University of Life and Environmental Sciences of Ukraine, Kyiv, Ukraine
Nadiia Bilko

Department of Laboratory Diagnostics of Biological Systems, Center of Molecular and Cell Research of National University of Kyiv-Mohyla Academy, Kyiv, Ukraine

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Plain Text BibTeX RIS

APA Style

Maksym Prylutskyi, Nickolaj Starodub, Nadiia Bilko. (2017). Determination of the Concentration of Polyamines with SPR-Based Immune Biosensor for Early Diagnostics of Breast Cancer. International Journal of Clinical Oncology and Cancer Research, 1(1), 30-35. https://doi.org/10.11648/j.ijcocr.20160101.15

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ACS Style

Maksym Prylutskyi; Nickolaj Starodub; Nadiia Bilko. Determination of the Concentration of Polyamines with SPR-Based Immune Biosensor for Early Diagnostics of Breast Cancer. Int. J. Clin. Oncol. Cancer Res. 2017, 1(1), 30-35. doi: 10.11648/j.ijcocr.20160101.15

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AMA Style

Maksym Prylutskyi, Nickolaj Starodub, Nadiia Bilko. Determination of the Concentration of Polyamines with SPR-Based Immune Biosensor for Early Diagnostics of Breast Cancer. Int J Clin Oncol Cancer Res. 2017;1(1):30-35. doi: 10.11648/j.ijcocr.20160101.15

Copy | Download

@article{10.11648/j.ijcocr.20160101.15,
  author = {Maksym Prylutskyi and Nickolaj Starodub and Nadiia Bilko},
  title = {Determination of the Concentration of Polyamines with SPR-Based Immune Biosensor for Early Diagnostics of Breast Cancer},
  journal = {International Journal of Clinical Oncology and Cancer Research},
  volume = {1},
  number = {1},
  pages = {30-35},
  doi = {10.11648/j.ijcocr.20160101.15},
  url = {https://doi.org/10.11648/j.ijcocr.20160101.15},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijcocr.20160101.15},
  abstract = {The paper presents the results of research on the development of immune biosensor test system for express detection of polyamines in cells of breast cancer. Determination of polyamines was performed by using an analytical device - immune biosensor based on surface plasmon resonance (SPR), where "antigen-antibody" reaction is performed in real time on the surface of transducer, resulting in formation of immune complexes and recording the shift of resonance angle. It was performed two methods of analysis: direct, when immobilized specific antibodies interacted with appropriate antigens and competitive, when the one part of antigens was conjugated with protein and competed for binding with specific antibodies with free antigens. In relation to direct analysis it was shown the dependence of sensitivity of biosensor response from the concentration of polyamines in range of 10-100 ng /ml. Concerning the competitive method of analysis, the linear region of sensitivity was in range of 0,3-80 ng/ml and 0,01-90 ng/ml for spermine and spermidine respectively.},
 year = {2017}
}

Copy | Download

TY  - JOUR
T1  - Determination of the Concentration of Polyamines with SPR-Based Immune Biosensor for Early Diagnostics of Breast Cancer
AU  - Maksym Prylutskyi
AU  - Nickolaj Starodub
AU  - Nadiia Bilko
Y1  - 2017/01/17
PY  - 2017
N1  - https://doi.org/10.11648/j.ijcocr.20160101.15
DO  - 10.11648/j.ijcocr.20160101.15
T2  - International Journal of Clinical Oncology and Cancer Research
JF  - International Journal of Clinical Oncology and Cancer Research
JO  - International Journal of Clinical Oncology and Cancer Research
SP  - 30
EP  - 35
PB  - Science Publishing Group
SN  - 2578-9511
UR  - https://doi.org/10.11648/j.ijcocr.20160101.15
AB  - The paper presents the results of research on the development of immune biosensor test system for express detection of polyamines in cells of breast cancer. Determination of polyamines was performed by using an analytical device - immune biosensor based on surface plasmon resonance (SPR), where "antigen-antibody" reaction is performed in real time on the surface of transducer, resulting in formation of immune complexes and recording the shift of resonance angle. It was performed two methods of analysis: direct, when immobilized specific antibodies interacted with appropriate antigens and competitive, when the one part of antigens was conjugated with protein and competed for binding with specific antibodies with free antigens. In relation to direct analysis it was shown the dependence of sensitivity of biosensor response from the concentration of polyamines in range of 10-100 ng /ml. Concerning the competitive method of analysis, the linear region of sensitivity was in range of 0,3-80 ng/ml and 0,01-90 ng/ml for spermine and spermidine respectively.
VL  - 1
IS  - 1
ER  -

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