Colorectal cancer affects a large number of people. There is an urgent need to develop novel treatments for this disease. In recent years, research on medical applications of atmospheric low temperature plasma (ALTP) has been actively conducted, and cancer treatment is one of the targets. In this study, we focused on colorectal cancer and evaluated plasma as a new treatment method. In vivo and in vitro experiments were conducted using plasma-treated saline (PTS) and plasma-treated medium (PTM) prepared by submerged bubbling treatment using ALTP. In vivo experiments using a rat model of colorectal cancer revealed that PTS administration to the colon slowed tumor progression based on endoscopic and histopathological observations. To investigate the cause of the inhibition of tumor progression, we evaluated the impact of ALTP on colon cancer cells. As a result of culturing colorectal cancer cells with PTM, cell proliferation was inhibited. Also, cell death was induced by cell swelling. qPCR revealed that PTMs induced cell death of cancer cells through signaling of tumor necrosis factor α (TNF-α), an inflammatory cytokine. Therefore, the inhibition of cancer progression by PTS in the rat model occurs by inducing cell death through the dissolution of ALTP components in the liquid.
| Published in | International Journal of Clinical Oncology and Cancer Research (Volume 7, Issue 1) |
| DOI | 10.11648/j.ijcocr.20220701.12 |
| Page(s) | 8-13 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2022. Published by Science Publishing Group |
Adenocarcinoma, Tumor Necrosis Factor-alpha, Therapeutics, Reactive Oxygen Species, Atmospheric Low Temperature Plasma
| [1] | Centers for Disease Control and Prevention. (2011). USCS Data Visualizations. Cdc.gov, Retrieved from https://gis.cdc.gov/Cancer/USCS/DataViz.html. |
| [2] | Bray, F., Ferlay, J., Soerjomataram, I., Siegel, R. L., Torre, L. A., & Jemal, A. (2018). Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA: A Cancer Journal for Clinicians, 68 (6), 394–424. |
| [3] | Divitiis, C. D., Nasti, G., Montano, M., Fisichella, R., Iaffaioli, R. V., & Berretta, M. (2014, November 7). Prognostic and predictive response factors in colorectal cancer patients: Between hope and reality. World Journal of Gastroenterology. 20 (41), 15049. |
| [4] | Matthes, R., Jablonowski, L., Holtfreter, B., Gerling, T., von Woedtke, T., & Kocher, T. (2019). Fibroblast Growth on Zirconia Ceramic and Titanium Disks After Application with Cold Atmospheric Pressure Plasma Devices or with Antiseptics. The International Journal of Oral & Maxillofacial Implants, 34 (4), 809–818. |
| [5] | Laroussi, M. (2005). Low Temperature Plasma-Based Sterilization: Overview and State-of-the-Art. Plasma Processes and Polymers, 2 (5), 391–400. |
| [6] | Bernhardt, T., Semmler, M. L., Schäfer, M., Bekeschus, S., Emmert, S., & Boeckmann, L. (2019). Plasma Medicine: Applications of Cold Atmospheric Pressure Plasma in Dermatology. Oxidative Medicine and Cellular Longevity, 2019, 1–10. |
| [7] | Heinlin, J., Isbary, G., Stolz, W., Morfill, G., Landthaler, M., Shimizu, T., Steffes, B., Nosenko, T., Zimmermann, J., & Karrer, S. (2010). Plasma applications in medicine with a special focus on dermatology. Journal of the European Academy of Dermatology and Venereology, 25 (1), 1–11. |
| [8] | Jones, D. P. (2008). Radical-free biology of oxidative stress. American Journal of Physiology-Cell Physiology, 295 (4), C849–C868. |
| [9] | Rehman, M. U., Jawaid, P., Uchiyama, H., & Kondo, T. (2016). Comparison of free radicals formation induced by cold atmospheric plasma, ultrasound, and ionizing radiation. Archives of Biochemistry and Biophysics, 605, 19–25. |
| [10] | Fridman, G., Friedman, G., Gutsol, A., Shekhter, A. B., Vasilets, V. N., & Fridman, A. (2008). Applied Plasma Medicine. Plasma Processes and Polymers, 5 (6), 503–533. |
| [11] | Takeda, S., Yamada, S., Hattori, N., Nakamura, K., Tanaka, H., Kajiyama, H., Kanda, M., Kobayashi, D., Tanaka, C., Fujii, T., Fujiwara, M., Mizuno, M., Hori, M., & Kodera, Y. (2017). Intraperitoneal Administration of Plasma-Activated Medium: Proposal of a Novel Treatment Option for Peritoneal Metastasis From Gastric Cancer. Annals of Surgical Oncology, 24 (5), 1188–1194. |
| [12] | Ghoneum, M., Gollapudi, A. (2009). Susceptibility of the human LNCaP prostate cancer cells to the apoptotic effect of marina crystal minerals (MCM) in vitro. Oncology Reports, 22 (01), 155-159. |
| [13] | Tanaka, H., Mizuno, M., Katsumata, Y., Ishikawa, K., Kondo, H., Hashizume, H., Okazaki, Y., Toyokuni, S., Nakamura, K., Yoshikawa, N., Kajiyama, H., Kikkawa, F., & Hori, M. (2019). Oxidative stress-dependent and -independent death of glioblastoma cells induced by non-thermal plasma-exposed solutions. Scientific Reports, 9 (1), Retrieved from https://doi.org/10.1038/s41598-019-50136-w. |
| [14] | Hirata, T., Kishimoto, T., Tsutsui, C., Kanai, T., & Mori, A. (2013). Healing burns using atmospheric pressure plasma irradiation. Japanese Journal of Applied Physics, 53 (1), Retrieved from https://doi.org/10.7567/jjap.53.010302. |
| [15] | Kochi, T., Shimizu, M., Shirakami, Y., Yoshimi, K., Kuramoto, T., Tanaka, T., & Moriwaki, H. (2015). Utility of Apc-mutant rats with a colitis-associated colon carcinogenesis model for chemoprevention studies. European Journal of Cancer Prevention, 24 (3), 180–187. |
| [16] | Irving, A. A., Yoshimi, K., Hart, M. L., Parker, T., Clipson, L., Ford, M. R., Kuramoto, T., Dove, W. F., & Amos-Landgraf, J. M. (2014). The utility ofApc-mutant rats in modeling human colon cancer. Disease Models & Mechanisms, 7 (11), 1215–1225. |
| [17] | Yoshimi, K., Tanaka, T., Takizawa, A., Kato, M., Hirabayashi, M., Mashimo, T., Serikawa, T., & Kuramoto, T. (2009). Enhanced colitis-associated colon carcinogenesis in a novelApcmutant rat. Cancer Science, 100 (11), 2022–2027. |
| [18] | Korinek, V., Barker, N., Morin P. J., Wichen, D., Weger, R., Kinzler, K. W., Vogelstein, B., & Cleverst, H. (1997). Constitutive Transcriptional Activation by a beta -Catenin-Tcf Complex in APC-/- Colon Carcinoma. Science, 275 (5307), 1784–1787. |
| [19] | Harada, N., Tamai, Y., Ishikawa, T.-o, Sauer, B., Takaku, K., Oshima, M., & Taketo, M. M. (1999). Intestinal polyposis in mice with a dominant stable mutation of the β-catenin gene. The EMBO Journal, 18 (21), 5931-5942. |
| [20] | Tazawa, H., Kawaguchi, T., Kobayashi, T., Kuramitsu, Y., Wada, S., Satomi, Y., Nishino, H., Kobayashi, M., Kanda, Y., Osaki, M., Kitagawa, T., Hosokawa, M., & Okada, F. (2013). Chronic inflammation-derived nitric oxide causes conversion of human colonic adenoma cells into adenocarcinoma cells. Experimental Cell Research, 319 (18), 2835–2844. |
| [21] | Tian, J., Cui, P., Li, Y., Yao, X., Wu, X., Wang, Z., & Li, C. (2020). LINC02418 promotes colon cancer progression by suppressing apoptosis via interaction with miR-34b-5p/BCL2 axis. Cancer Cell International, 20 (1), Retrieved from https://doi.org/10.1186/s12935-020-01530-2. |
| [22] | Morgan, M. J., Kim, Y.-S., & Liu, Z. (2008). TNFα and reactive oxygen species in necrotic cell death. Cell Research, 18 (3), 343–349. |
| [23] | Redza-Dutordoir, M., & Averill-Bates, D. A. (2016). Activation of apoptosis signalling pathways by reactive oxygen species. Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1863 (12), 2977–2992. |
| [24] | Van Antwerp, D. J., Martin, S. J., Kafri, T., Green, D. R., & Verma, I. M. (1996). Suppression of TNF-alpha -Induced Apoptosis by NF-kappa B. Science, 274 (5288), 787–789. |
| [25] | Kim, J. J., Lee, S. B., Park, J. K., & Yoo, Y. D. (2010). TNF- α -induced ROS production triggering apoptosis is directly linked to Romo1 and Bcl-X L. Cell Death & Differentiation, 17 (9), 1420–1434. |
| [26] | Kroemer, G., Petit, P., Zamzami, N., Vayssière, J., & Mignotte, B. (1995). The biochemistry of programmed cell death. The FASEB Journal, 9 (13), 1277–1287. |
| [27] | Glorieux, C., & Calderon, P. B. (2017). Catalase, a remarkable enzyme: targeting the oldest antioxidant enzyme to find a new cancer treatment approach. Biological Chemistry, 398 (10), 1095–1108. |
| [28] | Bertier, G., Carrot-Zhang, J., Ragoussis, V., & Joly, Y. (2016). Integrating precision cancer medicine into healthcare—policy, practice, and research challenges. Genome Medicine, 8 (1), Retrieved from https://doi.org/10.1186/s13073-016-0362-4. |
| [29] | KüminA., SchäferM., Epp, N., Bugnon, P., Born-Berclaz, C., Oxenius, A., Klippel, A., Bloch, W., & Werner, S. (2007). Peroxiredoxin 6 is required for blood vessel integrity in wounded skin. Journal of Cell Biology, 179 (4), 747–760. |
| [30] | Kümin, A., Huber, C., Rülicke, T., Wolf, E., & Werner, S. (2006). Peroxiredoxin 6 Is a Potent Cytoprotective Enzyme in the Epidermis. The American Journal of Pathology, 169 (4), 1194–1205. |
| [31] | Niethammer, P., Grabher, C., Look, A. T., & Mitchison, T. J. (2009). A tissue-scale gradient of hydrogen peroxide mediates rapid wound detection in zebrafish. Nature, 459 (7249), 996–999. |
| [32] | Li, J., Stouffs, M., Serrander, L., Banfi, B., Bettiol, E., Charnay, Y., Steger, K., Krause, K.-H., & Jaconi, M. E. (2006). The NADPH Oxidase NOX4 Drives Cardiac Differentiation: Role in Regulating Cardiac Transcription Factors and MAP Kinase Activation. Molecular Biology of the Cell, 17 (9), 3978–3988. |
| [33] | Országh, J., Danko, M., Ribar, A., & Matejčík, Š. (2012). Nitrogen second positive system studied by electron induced fluorescence. Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms, 279, 76–79. |
| [34] | Yang, Y., Zhang, Y., Liao, Z., Pei, X., & Wu, S. (2018). OH Radicals Distribution and Discharge Dynamics of an Atmospheric Pressure Plasma Jet Above Water Surface. IEEE Transactions on Radiation and Plasma Medical Sciences, 2 (3), 223–228. |
| [35] | Yue, Y., Pei, X., Gidon, D., Wu, F., Wu, S., & Lu, X. (2018). Investigation of plasma dynamics and spatially varying O and OH concentrations in atmospheric pressure plasma jets impinging on glass, water and metal substrates. Plasma Sources Science and Technology, 27 (6), Retrieved from https://doi.org/10.1088/1361-6595/aac618. |
APA Style
Genu Takahashi, Nanako Okuno, Kyota Yoshino, Takamichi Hirata, Chihiro Kobayashi, et al. (2022). The Effects of Plasma-Activated Saline and Medium on Colorectal Cancer in Rat Models and the Human Colon Adenocarcinoma Cell Line COLO 205. International Journal of Clinical Oncology and Cancer Research, 7(1), 8-13. https://doi.org/10.11648/j.ijcocr.20220701.12
ACS Style
Genu Takahashi; Nanako Okuno; Kyota Yoshino; Takamichi Hirata; Chihiro Kobayashi, et al. The Effects of Plasma-Activated Saline and Medium on Colorectal Cancer in Rat Models and the Human Colon Adenocarcinoma Cell Line COLO 205. Int. J. Clin. Oncol. Cancer Res. 2022, 7(1), 8-13. doi: 10.11648/j.ijcocr.20220701.12
AMA Style
Genu Takahashi, Nanako Okuno, Kyota Yoshino, Takamichi Hirata, Chihiro Kobayashi, et al. The Effects of Plasma-Activated Saline and Medium on Colorectal Cancer in Rat Models and the Human Colon Adenocarcinoma Cell Line COLO 205. Int J Clin Oncol Cancer Res. 2022;7(1):8-13. doi: 10.11648/j.ijcocr.20220701.12
Share This Article
Twitter
Linked In
Facebook
Copy
Download@article{10.11648/j.ijcocr.20220701.12,
author = {Genu Takahashi and Nanako Okuno and Kyota Yoshino and Takamichi Hirata and Chihiro Kobayashi and Akira Mori and Masaya Watada},
title = {The Effects of Plasma-Activated Saline and Medium on Colorectal Cancer in Rat Models and the Human Colon Adenocarcinoma Cell Line COLO 205},
journal = {International Journal of Clinical Oncology and Cancer Research},
volume = {7},
number = {1},
pages = {8-13},
doi = {10.11648/j.ijcocr.20220701.12},
url = {https://doi.org/10.11648/j.ijcocr.20220701.12},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijcocr.20220701.12},
abstract = {Colorectal cancer affects a large number of people. There is an urgent need to develop novel treatments for this disease. In recent years, research on medical applications of atmospheric low temperature plasma (ALTP) has been actively conducted, and cancer treatment is one of the targets. In this study, we focused on colorectal cancer and evaluated plasma as a new treatment method. In vivo and in vitro experiments were conducted using plasma-treated saline (PTS) and plasma-treated medium (PTM) prepared by submerged bubbling treatment using ALTP. In vivo experiments using a rat model of colorectal cancer revealed that PTS administration to the colon slowed tumor progression based on endoscopic and histopathological observations. To investigate the cause of the inhibition of tumor progression, we evaluated the impact of ALTP on colon cancer cells. As a result of culturing colorectal cancer cells with PTM, cell proliferation was inhibited. Also, cell death was induced by cell swelling. qPCR revealed that PTMs induced cell death of cancer cells through signaling of tumor necrosis factor α (TNF-α), an inflammatory cytokine. Therefore, the inhibition of cancer progression by PTS in the rat model occurs by inducing cell death through the dissolution of ALTP components in the liquid.},
year = {2022}
}
TY - JOUR T1 - The Effects of Plasma-Activated Saline and Medium on Colorectal Cancer in Rat Models and the Human Colon Adenocarcinoma Cell Line COLO 205 AU - Genu Takahashi AU - Nanako Okuno AU - Kyota Yoshino AU - Takamichi Hirata AU - Chihiro Kobayashi AU - Akira Mori AU - Masaya Watada Y1 - 2022/02/09 PY - 2022 N1 - https://doi.org/10.11648/j.ijcocr.20220701.12 DO - 10.11648/j.ijcocr.20220701.12 T2 - International Journal of Clinical Oncology and Cancer Research JF - International Journal of Clinical Oncology and Cancer Research JO - International Journal of Clinical Oncology and Cancer Research SP - 8 EP - 13 PB - Science Publishing Group SN - 2578-9511 UR - https://doi.org/10.11648/j.ijcocr.20220701.12 AB - Colorectal cancer affects a large number of people. There is an urgent need to develop novel treatments for this disease. In recent years, research on medical applications of atmospheric low temperature plasma (ALTP) has been actively conducted, and cancer treatment is one of the targets. In this study, we focused on colorectal cancer and evaluated plasma as a new treatment method. In vivo and in vitro experiments were conducted using plasma-treated saline (PTS) and plasma-treated medium (PTM) prepared by submerged bubbling treatment using ALTP. In vivo experiments using a rat model of colorectal cancer revealed that PTS administration to the colon slowed tumor progression based on endoscopic and histopathological observations. To investigate the cause of the inhibition of tumor progression, we evaluated the impact of ALTP on colon cancer cells. As a result of culturing colorectal cancer cells with PTM, cell proliferation was inhibited. Also, cell death was induced by cell swelling. qPCR revealed that PTMs induced cell death of cancer cells through signaling of tumor necrosis factor α (TNF-α), an inflammatory cytokine. Therefore, the inhibition of cancer progression by PTS in the rat model occurs by inducing cell death through the dissolution of ALTP components in the liquid. VL - 7 IS - 1 ER -
Information
Email: