The purpose of this study was to investigate differences in the interface between new bioactive glasses (RKKP, RBP1, RBP2) and mirror-polished titanium alloys in the evaluation of human osteoblast cells (HOCs) by TEM. Mirror-polished titanium alloy (MTi), bioactive glass (RKKP, RBP1, RBP2), and plastic culture dishes (Falcon [F]; used as a control) were used in this study. 1.0×105 HOCs were plated on each of the materials and cultured for 1 week. For TEM, HOCs were fixed in 2.5% glutaraldehyde, post-fixed in 1% osmium tetroxide, dehydrated, and embedded in Epon 812. After polymerization for 48 hours, the sample was removed with liquid nitrogen, and the specimen was re-embedded in Epon 812, sliced into 200-nm-thick semithin sections, and stained with a mixture of 1% toluidine blue, 1% Azur II, and 1% borax. After confirming the presence of cells, the specimens were sliced at a thickness of approximately 78 nm, double-stained with uranyl acetate and lead citrate, and examined under a TEM. As results, In Falcon, we found an extremer on the material side, focal contact, and close contact in parts of the plate. In M-Ti, we observed a non-structured homogeneous layer. In RBP1 and RBP2, an intervening layer with a gel like condition of approximately 100 nm in thickness was observed. In RKKP the collagen fibers bonded directly, indicating the presence of a bone matrix of HOCs at all sites. In conclusion, the interface between the different bioactive materials and the bio-inactive material showed completely different phenomena. Furthermore, even the same bioactive materials showed different patterns because of their different composition. From these results, we concluded that RKKP might be the best biomaterial because it bonded directly to human osteoblast cells without an intervening layer.
| Published in | International Journal of Dental Medicine (Volume 4, Issue 1) |
| DOI | 10.11648/j.ijdm.20180401.15 |
| Page(s) | 19-26 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2018. Published by Science Publishing Group |
Bioactive Bioglass, Human Osteoblast Cells, Interface
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APA Style
Naho Akamatsu, Noriko Suzuki, Wataru Ofusa, Yukimi Suzuki, Hitoshi Oguchi. (2018). Observing the Responses of the Interface Between New Bioactive Glasses and Human Osteoblast Cells (HOCs) by TEM. International Journal of Dental Medicine, 4(1), 19-26. https://doi.org/10.11648/j.ijdm.20180401.15
ACS Style
Naho Akamatsu; Noriko Suzuki; Wataru Ofusa; Yukimi Suzuki; Hitoshi Oguchi. Observing the Responses of the Interface Between New Bioactive Glasses and Human Osteoblast Cells (HOCs) by TEM. Int. J. Dent. Med. 2018, 4(1), 19-26. doi: 10.11648/j.ijdm.20180401.15
AMA Style
Naho Akamatsu, Noriko Suzuki, Wataru Ofusa, Yukimi Suzuki, Hitoshi Oguchi. Observing the Responses of the Interface Between New Bioactive Glasses and Human Osteoblast Cells (HOCs) by TEM. Int J Dent Med. 2018;4(1):19-26. doi: 10.11648/j.ijdm.20180401.15
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Download@article{10.11648/j.ijdm.20180401.15,
author = {Naho Akamatsu and Noriko Suzuki and Wataru Ofusa and Yukimi Suzuki and Hitoshi Oguchi},
title = {Observing the Responses of the Interface Between New Bioactive Glasses and Human Osteoblast Cells (HOCs) by TEM},
journal = {International Journal of Dental Medicine},
volume = {4},
number = {1},
pages = {19-26},
doi = {10.11648/j.ijdm.20180401.15},
url = {https://doi.org/10.11648/j.ijdm.20180401.15},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijdm.20180401.15},
abstract = {The purpose of this study was to investigate differences in the interface between new bioactive glasses (RKKP, RBP1, RBP2) and mirror-polished titanium alloys in the evaluation of human osteoblast cells (HOCs) by TEM. Mirror-polished titanium alloy (MTi), bioactive glass (RKKP, RBP1, RBP2), and plastic culture dishes (Falcon [F]; used as a control) were used in this study. 1.0×105 HOCs were plated on each of the materials and cultured for 1 week. For TEM, HOCs were fixed in 2.5% glutaraldehyde, post-fixed in 1% osmium tetroxide, dehydrated, and embedded in Epon 812. After polymerization for 48 hours, the sample was removed with liquid nitrogen, and the specimen was re-embedded in Epon 812, sliced into 200-nm-thick semithin sections, and stained with a mixture of 1% toluidine blue, 1% Azur II, and 1% borax. After confirming the presence of cells, the specimens were sliced at a thickness of approximately 78 nm, double-stained with uranyl acetate and lead citrate, and examined under a TEM. As results, In Falcon, we found an extremer on the material side, focal contact, and close contact in parts of the plate. In M-Ti, we observed a non-structured homogeneous layer. In RBP1 and RBP2, an intervening layer with a gel like condition of approximately 100 nm in thickness was observed. In RKKP the collagen fibers bonded directly, indicating the presence of a bone matrix of HOCs at all sites. In conclusion, the interface between the different bioactive materials and the bio-inactive material showed completely different phenomena. Furthermore, even the same bioactive materials showed different patterns because of their different composition. From these results, we concluded that RKKP might be the best biomaterial because it bonded directly to human osteoblast cells without an intervening layer.},
year = {2018}
}
TY - JOUR T1 - Observing the Responses of the Interface Between New Bioactive Glasses and Human Osteoblast Cells (HOCs) by TEM AU - Naho Akamatsu AU - Noriko Suzuki AU - Wataru Ofusa AU - Yukimi Suzuki AU - Hitoshi Oguchi Y1 - 2018/08/04 PY - 2018 N1 - https://doi.org/10.11648/j.ijdm.20180401.15 DO - 10.11648/j.ijdm.20180401.15 T2 - International Journal of Dental Medicine JF - International Journal of Dental Medicine JO - International Journal of Dental Medicine SP - 19 EP - 26 PB - Science Publishing Group SN - 2472-1387 UR - https://doi.org/10.11648/j.ijdm.20180401.15 AB - The purpose of this study was to investigate differences in the interface between new bioactive glasses (RKKP, RBP1, RBP2) and mirror-polished titanium alloys in the evaluation of human osteoblast cells (HOCs) by TEM. Mirror-polished titanium alloy (MTi), bioactive glass (RKKP, RBP1, RBP2), and plastic culture dishes (Falcon [F]; used as a control) were used in this study. 1.0×105 HOCs were plated on each of the materials and cultured for 1 week. For TEM, HOCs were fixed in 2.5% glutaraldehyde, post-fixed in 1% osmium tetroxide, dehydrated, and embedded in Epon 812. After polymerization for 48 hours, the sample was removed with liquid nitrogen, and the specimen was re-embedded in Epon 812, sliced into 200-nm-thick semithin sections, and stained with a mixture of 1% toluidine blue, 1% Azur II, and 1% borax. After confirming the presence of cells, the specimens were sliced at a thickness of approximately 78 nm, double-stained with uranyl acetate and lead citrate, and examined under a TEM. As results, In Falcon, we found an extremer on the material side, focal contact, and close contact in parts of the plate. In M-Ti, we observed a non-structured homogeneous layer. In RBP1 and RBP2, an intervening layer with a gel like condition of approximately 100 nm in thickness was observed. In RKKP the collagen fibers bonded directly, indicating the presence of a bone matrix of HOCs at all sites. In conclusion, the interface between the different bioactive materials and the bio-inactive material showed completely different phenomena. Furthermore, even the same bioactive materials showed different patterns because of their different composition. From these results, we concluded that RKKP might be the best biomaterial because it bonded directly to human osteoblast cells without an intervening layer. VL - 4 IS - 1 ER -
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