Ectomycorrhiza Formation of <i>Tricholoma matsutake</i> on Mature Pine Root in Situ

Yong Chan Kim; Kol Nam; Myong Song Kim; Un Chol Kim; Un Hyok Kim; Myong Bok Kim; Gum Chol Pak; Hui Won Kim

doi:doi:10.11648/j.ijee.20230803.13

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Ectomycorrhiza Formation of Tricholoma matsutake on Mature Pine Root in Situ

Yong Chan Kim, Kol Nam, Myong Song Kim, Un Chol Kim, Un Hyok Kim, Myong Bok Kim, Gum Chol Pak, Hui Won Kim

Published in International Journal of Ecotoxicology and Ecobiology (Volume 8, Issue 3)

Received: 26 July 2023 Accepted: 4 September 2023 Published: 18 September 2023

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Abstract

We studied on the ectomycorrhiza formation of Tricholoma matsutake on mature pine (Pinus densiflora) root using matsutake solid spawn in situ. To find out suitable medium for the solid spawn, the matsutake mycelium growths on different media were compared. Among them, GYP medium (glucose 20g, yeast extract 5g, peptone 5g, KH₂PO₄ 1g, CaCl₂ 50mg and vitamin B₁ 50mg) and GWR medium (glucose 20g, wheat bran 50g, rice bran 50g, KH₂PO₄ 1g, CaCl₂ 50mg and vitamin B₁ 50mg) showed good proliferation of matsutake mycelium. The GYP and GWR media were used to make charcoal and vermiculite-soil spawns. The ergosterol amounts of charcoal and vermiculite-soil spawns were 1.53 and 1.61 times higher than that of the natural shiro, respectively. In spring, the roots of about 30-year-old red pine were shortened with unsterilized scissors. About twelve months later, the regenerated root tips from shortened points were inoculated with charcoal and vermiculite-soil spawns, respectively. After four months by the inoculation with charcoal and vermiculite-soil spawns, 55% and 50% of experimental plots inoculated with charcoal and vermiculite-soil spawns revealed dichotomic root tips characteristic to ectomycorrhiza, respectively. The nested PCR amplification results confirmed that the dichotomous root tips were colonised by matsutake spawn. Our findings demonstrated that the ectomycorrhiza of T. matsutake could be formed on the mature pine root in situ by using matsutake solid spawns.

Published in	International Journal of Ecotoxicology and Ecobiology (Volume 8, Issue 3)
DOI	10.11648/j.ijee.20230803.13
Page(s)	41-48
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2023. Published by Science Publishing Group

Keywords

Tricholoma matsutake, Ectomycorrhiza, Solid Spawn, Inoculation, Pinus desiflora, Nested PCR

References

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[12]	VAARIO L. M., GUERIN-LAGUETTE A., GILL W. M., LAPEYRIE F., AND SUZUKI K. 2000. Only two weeks are required for Tricholoma matsutake to differentiate ectomycorrhizal Hartig net structures in roots of Pinus densiflora seedlings cultivated on artificial substrate. Journal of Forest Research. 5: 293-297.
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[20]	KIKUCHI K., MATSUSHITA N., GUERIN-LAGUETTE A., OHTA A. AND SUZUKI K. 2000. Detection of Tricholoma matsutakeby specific primers. Mycol. Res. 104: 1427-1430.
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[23]	Kawai M., IMAJI A., YAMADA A., AND KINOSHITA A. 2018. Shiro formation and fruit body flashing by inoculation of Tricholoma bakamatsutake mycelia in the forest. P. 25 in Abstracts of the annual meeting of the mycological Society of Japan. https://doi.org/10.11556/msj7abst.62.0_25.

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APA Style

Yong Chan Kim, Kol Nam, Myong Song Kim, Un Chol Kim, Un Hyok Kim, et al. (2023). Ectomycorrhiza Formation of Tricholoma matsutake on Mature Pine Root in Situ. International Journal of Ecotoxicology and Ecobiology, 8(3), 41-48. https://doi.org/10.11648/j.ijee.20230803.13

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ACS Style

Yong Chan Kim; Kol Nam; Myong Song Kim; Un Chol Kim; Un Hyok Kim, et al. Ectomycorrhiza Formation of Tricholoma matsutake on Mature Pine Root in Situ. Int. J. Ecotoxicol. Ecobiol. 2023, 8(3), 41-48. doi: 10.11648/j.ijee.20230803.13

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AMA Style

Yong Chan Kim, Kol Nam, Myong Song Kim, Un Chol Kim, Un Hyok Kim, et al. Ectomycorrhiza Formation of Tricholoma matsutake on Mature Pine Root in Situ. Int J Ecotoxicol Ecobiol. 2023;8(3):41-48. doi: 10.11648/j.ijee.20230803.13

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@article{10.11648/j.ijee.20230803.13,
  author = {Yong Chan Kim and Kol Nam and Myong Song Kim and Un Chol Kim and Un Hyok Kim and Myong Bok Kim and Gum Chol Pak and Hui Won Kim},
  title = {Ectomycorrhiza Formation of Tricholoma matsutake on Mature Pine Root in Situ},
  journal = {International Journal of Ecotoxicology and Ecobiology},
  volume = {8},
  number = {3},
  pages = {41-48},
  doi = {10.11648/j.ijee.20230803.13},
  url = {https://doi.org/10.11648/j.ijee.20230803.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijee.20230803.13},
  abstract = {We studied on the ectomycorrhiza formation of Tricholoma matsutake on mature pine (Pinus densiflora) root using matsutake solid spawn in situ. To find out suitable medium for the solid spawn, the matsutake mycelium growths on different media were compared. Among them, GYP medium (glucose 20g, yeast extract 5g, peptone 5g, KH2PO4 1g, CaCl2 50mg and vitamin B1 50mg) and GWR medium (glucose 20g, wheat bran 50g, rice bran 50g, KH2PO4 1g, CaCl2 50mg and vitamin B1 50mg) showed good proliferation of matsutake mycelium. The GYP and GWR media were used to make charcoal and vermiculite-soil spawns. The ergosterol amounts of charcoal and vermiculite-soil spawns were 1.53 and 1.61 times higher than that of the natural shiro, respectively. In spring, the roots of about 30-year-old red pine were shortened with unsterilized scissors. About twelve months later, the regenerated root tips from shortened points were inoculated with charcoal and vermiculite-soil spawns, respectively. After four months by the inoculation with charcoal and vermiculite-soil spawns, 55% and 50% of experimental plots inoculated with charcoal and vermiculite-soil spawns revealed dichotomic root tips characteristic to ectomycorrhiza, respectively. The nested PCR amplification results confirmed that the dichotomous root tips were colonised by matsutake spawn. Our findings demonstrated that the ectomycorrhiza of T. matsutake could be formed on the mature pine root in situ by using matsutake solid spawns.},
 year = {2023}
}

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TY - JOUR
T1 - Ectomycorrhiza Formation of Tricholoma matsutake on Mature Pine Root in Situ
AU - Yong Chan Kim
AU - Kol Nam
AU - Myong Song Kim
AU - Un Chol Kim
AU - Un Hyok Kim
AU - Myong Bok Kim
AU - Gum Chol Pak
AU - Hui Won Kim
Y1 - 2023/09/18
PY - 2023
N1 - https://doi.org/10.11648/j.ijee.20230803.13
DO - 10.11648/j.ijee.20230803.13
T2 - International Journal of Ecotoxicology and Ecobiology
JF - International Journal of Ecotoxicology and Ecobiology
JO - International Journal of Ecotoxicology and Ecobiology
SP - 41
EP - 48
PB - Science Publishing Group
SN - 2575-1735
UR - https://doi.org/10.11648/j.ijee.20230803.13
AB - We studied on the ectomycorrhiza formation of Tricholoma matsutake on mature pine (Pinus densiflora) root using matsutake solid spawn in situ. To find out suitable medium for the solid spawn, the matsutake mycelium growths on different media were compared. Among them, GYP medium (glucose 20g, yeast extract 5g, peptone 5g, KH2PO4 1g, CaCl2 50mg and vitamin B1 50mg) and GWR medium (glucose 20g, wheat bran 50g, rice bran 50g, KH2PO4 1g, CaCl2 50mg and vitamin B1 50mg) showed good proliferation of matsutake mycelium. The GYP and GWR media were used to make charcoal and vermiculite-soil spawns. The ergosterol amounts of charcoal and vermiculite-soil spawns were 1.53 and 1.61 times higher than that of the natural shiro, respectively. In spring, the roots of about 30-year-old red pine were shortened with unsterilized scissors. About twelve months later, the regenerated root tips from shortened points were inoculated with charcoal and vermiculite-soil spawns, respectively. After four months by the inoculation with charcoal and vermiculite-soil spawns, 55% and 50% of experimental plots inoculated with charcoal and vermiculite-soil spawns revealed dichotomic root tips characteristic to ectomycorrhiza, respectively. The nested PCR amplification results confirmed that the dichotomous root tips were colonised by matsutake spawn. Our findings demonstrated that the ectomycorrhiza of T. matsutake could be formed on the mature pine root in situ by using matsutake solid spawns.
VL - 8
IS - 3
ER -

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Author Information

Yong Chan Kim

Laboratory of Matsutake, Central Institute of Mushroom, Agricultural Committee, Pyongyang, Korea
Kol Nam

Laboratory of Matsutake, Central Institute of Mushroom, Agricultural Committee, Pyongyang, Korea
Myong Song Kim

Laboratory of Matsutake, Central Institute of Mushroom, Agricultural Committee, Pyongyang, Korea
Un Chol Kim

Laboratory of Matsutake, Central Institute of Mushroom, Agricultural Committee, Pyongyang, Korea
Un Hyok Kim

Laboratory of Matsutake, Central Institute of Mushroom, Agricultural Committee, Pyongyang, Korea
Myong Bok Kim

Laboratory of Matsutake, Central Institute of Mushroom, Agricultural Committee, Pyongyang, Korea
Gum Chol Pak

Laboratory of Matsutake, Central Institute of Mushroom, Agricultural Committee, Pyongyang, Korea
Hui Won Kim

Department of Daily Food, Institute of Microbiology, The State Academy of Sciences, Pyongyang, Korea

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Plain Text BibTeX RIS

APA Style

Yong Chan Kim, Kol Nam, Myong Song Kim, Un Chol Kim, Un Hyok Kim, et al. (2023). Ectomycorrhiza Formation of Tricholoma matsutake on Mature Pine Root in Situ. International Journal of Ecotoxicology and Ecobiology, 8(3), 41-48. https://doi.org/10.11648/j.ijee.20230803.13

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ACS Style

Yong Chan Kim; Kol Nam; Myong Song Kim; Un Chol Kim; Un Hyok Kim, et al. Ectomycorrhiza Formation of Tricholoma matsutake on Mature Pine Root in Situ. Int. J. Ecotoxicol. Ecobiol. 2023, 8(3), 41-48. doi: 10.11648/j.ijee.20230803.13

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AMA Style

Yong Chan Kim, Kol Nam, Myong Song Kim, Un Chol Kim, Un Hyok Kim, et al. Ectomycorrhiza Formation of Tricholoma matsutake on Mature Pine Root in Situ. Int J Ecotoxicol Ecobiol. 2023;8(3):41-48. doi: 10.11648/j.ijee.20230803.13

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@article{10.11648/j.ijee.20230803.13,
  author = {Yong Chan Kim and Kol Nam and Myong Song Kim and Un Chol Kim and Un Hyok Kim and Myong Bok Kim and Gum Chol Pak and Hui Won Kim},
  title = {Ectomycorrhiza Formation of Tricholoma matsutake on Mature Pine Root in Situ},
  journal = {International Journal of Ecotoxicology and Ecobiology},
  volume = {8},
  number = {3},
  pages = {41-48},
  doi = {10.11648/j.ijee.20230803.13},
  url = {https://doi.org/10.11648/j.ijee.20230803.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijee.20230803.13},
  abstract = {We studied on the ectomycorrhiza formation of Tricholoma matsutake on mature pine (Pinus densiflora) root using matsutake solid spawn in situ. To find out suitable medium for the solid spawn, the matsutake mycelium growths on different media were compared. Among them, GYP medium (glucose 20g, yeast extract 5g, peptone 5g, KH2PO4 1g, CaCl2 50mg and vitamin B1 50mg) and GWR medium (glucose 20g, wheat bran 50g, rice bran 50g, KH2PO4 1g, CaCl2 50mg and vitamin B1 50mg) showed good proliferation of matsutake mycelium. The GYP and GWR media were used to make charcoal and vermiculite-soil spawns. The ergosterol amounts of charcoal and vermiculite-soil spawns were 1.53 and 1.61 times higher than that of the natural shiro, respectively. In spring, the roots of about 30-year-old red pine were shortened with unsterilized scissors. About twelve months later, the regenerated root tips from shortened points were inoculated with charcoal and vermiculite-soil spawns, respectively. After four months by the inoculation with charcoal and vermiculite-soil spawns, 55% and 50% of experimental plots inoculated with charcoal and vermiculite-soil spawns revealed dichotomic root tips characteristic to ectomycorrhiza, respectively. The nested PCR amplification results confirmed that the dichotomous root tips were colonised by matsutake spawn. Our findings demonstrated that the ectomycorrhiza of T. matsutake could be formed on the mature pine root in situ by using matsutake solid spawns.},
 year = {2023}
}

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