Electron Microscopic Studies in <i>Escherichia Coli</i> on Mode of Action of Sodium Benzoate and Potassium Sorbate

Norma Angélica Santiesteban-López; Teresa Gladys Cerón-Carrillo; José Luis Carmona-Silva; Javier Castro-Rosas

doi:doi:10.11648/j.ijfsb.20190404.11

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Electron Microscopic Studies in Escherichia Coli on Mode of Action of Sodium Benzoate and Potassium Sorbate

Norma Angélica Santiesteban-López, Teresa Gladys Cerón-Carrillo, José Luis Carmona-Silva, Javier Castro-Rosas

Published in International Journal of Food Science and Biotechnology (Volume 4, Issue 4)

Received: 11 March 2019 Accepted: 15 May 2019 Published: 24 October 2019

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Abstract

Traditionally food antimicrobials was utilized to extent the lag phase or inhibit the growth of microorganisms; however, it has been demonstrated that exposure to antimicrobials such as sodium benzoate and potassium sorbate in sublethal concentrations, and gradually increasing the dose, allowed the adaptation of microorganisms of interest in food, such as E. coli, exhibiting induced resistance by unknown mechanisms. Therefore, the objective of this study was to identify the ultrastructural changes in viable cells of E. coli adapted to high concentrations (7000 ppm) of these antimicrobials, using transmission electron microscopy (TEM). After treatment with potassium sorbate, E. coli presented important morphological changes such as the separation of the cell membrane from the cytoplasm and cell wall, the appearance of a remarkable electronic light at the center of cells containing condensed deoxyribonucleic acid (DNA) molecules, as well as the appearance of small dense granules of electrons. Therefores, potassium sorbate induced more severe shape structural changes, presence of unusual structures and loss of integrity compared to viable cells adapted to sodium benzoate.

Published in	International Journal of Food Science and Biotechnology (Volume 4, Issue 4)
DOI	10.11648/j.ijfsb.20190404.11
Page(s)	82-86
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2019. Published by Science Publishing Group

Keywords

Adaptation, Potassium Sorbate, Sodium Benzoate, E. coli, Morphological Changes

References

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APA Style

Norma Angélica Santiesteban-López, Teresa Gladys Cerón-Carrillo, José Luis Carmona-Silva, Javier Castro-Rosas. (2019). Electron Microscopic Studies in Escherichia Coli on Mode of Action of Sodium Benzoate and Potassium Sorbate. International Journal of Food Science and Biotechnology, 4(4), 82-86. https://doi.org/10.11648/j.ijfsb.20190404.11

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ACS Style

Norma Angélica Santiesteban-López; Teresa Gladys Cerón-Carrillo; José Luis Carmona-Silva; Javier Castro-Rosas. Electron Microscopic Studies in Escherichia Coli on Mode of Action of Sodium Benzoate and Potassium Sorbate. Int. J. Food Sci. Biotechnol. 2019, 4(4), 82-86. doi: 10.11648/j.ijfsb.20190404.11

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AMA Style

Norma Angélica Santiesteban-López, Teresa Gladys Cerón-Carrillo, José Luis Carmona-Silva, Javier Castro-Rosas. Electron Microscopic Studies in Escherichia Coli on Mode of Action of Sodium Benzoate and Potassium Sorbate. Int J Food Sci Biotechnol. 2019;4(4):82-86. doi: 10.11648/j.ijfsb.20190404.11

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@article{10.11648/j.ijfsb.20190404.11,
  author = {Norma Angélica Santiesteban-López and Teresa Gladys Cerón-Carrillo and José Luis Carmona-Silva and Javier Castro-Rosas},
  title = {Electron Microscopic Studies in Escherichia Coli on Mode of Action of Sodium Benzoate and Potassium Sorbate},
  journal = {International Journal of Food Science and Biotechnology},
  volume = {4},
  number = {4},
  pages = {82-86},
  doi = {10.11648/j.ijfsb.20190404.11},
  url = {https://doi.org/10.11648/j.ijfsb.20190404.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijfsb.20190404.11},
  abstract = {Traditionally food antimicrobials was utilized to extent the lag phase or inhibit the growth of microorganisms; however, it has been demonstrated that exposure to antimicrobials such as sodium benzoate and potassium sorbate in sublethal concentrations, and gradually increasing the dose, allowed the adaptation of microorganisms of interest in food, such as E. coli, exhibiting induced resistance by unknown mechanisms. Therefore, the objective of this study was to identify the ultrastructural changes in viable cells of E. coli adapted to high concentrations (7000 ppm) of these antimicrobials, using transmission electron microscopy (TEM). After treatment with potassium sorbate, E. coli presented important morphological changes such as the separation of the cell membrane from the cytoplasm and cell wall, the appearance of a remarkable electronic light at the center of cells containing condensed deoxyribonucleic acid (DNA) molecules, as well as the appearance of small dense granules of electrons. Therefores, potassium sorbate induced more severe shape structural changes, presence of unusual structures and loss of integrity compared to viable cells adapted to sodium benzoate.},
 year = {2019}
}

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TY  - JOUR
T1  - Electron Microscopic Studies in Escherichia Coli on Mode of Action of Sodium Benzoate and Potassium Sorbate
AU  - Norma Angélica Santiesteban-López
AU  - Teresa Gladys Cerón-Carrillo
AU  - José Luis Carmona-Silva
AU  - Javier Castro-Rosas
Y1  - 2019/10/24
PY  - 2019
N1  - https://doi.org/10.11648/j.ijfsb.20190404.11
DO  - 10.11648/j.ijfsb.20190404.11
T2  - International Journal of Food Science and Biotechnology
JF  - International Journal of Food Science and Biotechnology
JO  - International Journal of Food Science and Biotechnology
SP  - 82
EP  - 86
PB  - Science Publishing Group
SN  - 2578-9643
UR  - https://doi.org/10.11648/j.ijfsb.20190404.11
AB  - Traditionally food antimicrobials was utilized to extent the lag phase or inhibit the growth of microorganisms; however, it has been demonstrated that exposure to antimicrobials such as sodium benzoate and potassium sorbate in sublethal concentrations, and gradually increasing the dose, allowed the adaptation of microorganisms of interest in food, such as E. coli, exhibiting induced resistance by unknown mechanisms. Therefore, the objective of this study was to identify the ultrastructural changes in viable cells of E. coli adapted to high concentrations (7000 ppm) of these antimicrobials, using transmission electron microscopy (TEM). After treatment with potassium sorbate, E. coli presented important morphological changes such as the separation of the cell membrane from the cytoplasm and cell wall, the appearance of a remarkable electronic light at the center of cells containing condensed deoxyribonucleic acid (DNA) molecules, as well as the appearance of small dense granules of electrons. Therefores, potassium sorbate induced more severe shape structural changes, presence of unusual structures and loss of integrity compared to viable cells adapted to sodium benzoate.
VL  - 4
IS  - 4
ER  -

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Author Information

Norma Angélica Santiesteban-López

Faculty of Administration (Gastronomy). Benemérita Universidad Autónoma de Puebla, University City, Puebla, México
Teresa Gladys Cerón-Carrillo

Faculty of Administration (Gastronomy). Benemérita Universidad Autónoma de Puebla, University City, Puebla, México
José Luis Carmona-Silva

Faculty of Administration (Gastronomy). Benemérita Universidad Autónoma de Puebla, University City, Puebla, México
Javier Castro-Rosas

Academic Area of Chemistry, Institute of Basic Sciences and Engineering, Autonomous University of the State of Hidalgo, Hidalgo, México

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APA Style

Norma Angélica Santiesteban-López, Teresa Gladys Cerón-Carrillo, José Luis Carmona-Silva, Javier Castro-Rosas. (2019). Electron Microscopic Studies in Escherichia Coli on Mode of Action of Sodium Benzoate and Potassium Sorbate. International Journal of Food Science and Biotechnology, 4(4), 82-86. https://doi.org/10.11648/j.ijfsb.20190404.11

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ACS Style

Norma Angélica Santiesteban-López; Teresa Gladys Cerón-Carrillo; José Luis Carmona-Silva; Javier Castro-Rosas. Electron Microscopic Studies in Escherichia Coli on Mode of Action of Sodium Benzoate and Potassium Sorbate. Int. J. Food Sci. Biotechnol. 2019, 4(4), 82-86. doi: 10.11648/j.ijfsb.20190404.11

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AMA Style

Norma Angélica Santiesteban-López, Teresa Gladys Cerón-Carrillo, José Luis Carmona-Silva, Javier Castro-Rosas. Electron Microscopic Studies in Escherichia Coli on Mode of Action of Sodium Benzoate and Potassium Sorbate. Int J Food Sci Biotechnol. 2019;4(4):82-86. doi: 10.11648/j.ijfsb.20190404.11

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@article{10.11648/j.ijfsb.20190404.11,
  author = {Norma Angélica Santiesteban-López and Teresa Gladys Cerón-Carrillo and José Luis Carmona-Silva and Javier Castro-Rosas},
  title = {Electron Microscopic Studies in Escherichia Coli on Mode of Action of Sodium Benzoate and Potassium Sorbate},
  journal = {International Journal of Food Science and Biotechnology},
  volume = {4},
  number = {4},
  pages = {82-86},
  doi = {10.11648/j.ijfsb.20190404.11},
  url = {https://doi.org/10.11648/j.ijfsb.20190404.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijfsb.20190404.11},
  abstract = {Traditionally food antimicrobials was utilized to extent the lag phase or inhibit the growth of microorganisms; however, it has been demonstrated that exposure to antimicrobials such as sodium benzoate and potassium sorbate in sublethal concentrations, and gradually increasing the dose, allowed the adaptation of microorganisms of interest in food, such as E. coli, exhibiting induced resistance by unknown mechanisms. Therefore, the objective of this study was to identify the ultrastructural changes in viable cells of E. coli adapted to high concentrations (7000 ppm) of these antimicrobials, using transmission electron microscopy (TEM). After treatment with potassium sorbate, E. coli presented important morphological changes such as the separation of the cell membrane from the cytoplasm and cell wall, the appearance of a remarkable electronic light at the center of cells containing condensed deoxyribonucleic acid (DNA) molecules, as well as the appearance of small dense granules of electrons. Therefores, potassium sorbate induced more severe shape structural changes, presence of unusual structures and loss of integrity compared to viable cells adapted to sodium benzoate.},
 year = {2019}
}

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TY  - JOUR
T1  - Electron Microscopic Studies in Escherichia Coli on Mode of Action of Sodium Benzoate and Potassium Sorbate
AU  - Norma Angélica Santiesteban-López
AU  - Teresa Gladys Cerón-Carrillo
AU  - José Luis Carmona-Silva
AU  - Javier Castro-Rosas
Y1  - 2019/10/24
PY  - 2019
N1  - https://doi.org/10.11648/j.ijfsb.20190404.11
DO  - 10.11648/j.ijfsb.20190404.11
T2  - International Journal of Food Science and Biotechnology
JF  - International Journal of Food Science and Biotechnology
JO  - International Journal of Food Science and Biotechnology
SP  - 82
EP  - 86
PB  - Science Publishing Group
SN  - 2578-9643
UR  - https://doi.org/10.11648/j.ijfsb.20190404.11
AB  - Traditionally food antimicrobials was utilized to extent the lag phase or inhibit the growth of microorganisms; however, it has been demonstrated that exposure to antimicrobials such as sodium benzoate and potassium sorbate in sublethal concentrations, and gradually increasing the dose, allowed the adaptation of microorganisms of interest in food, such as E. coli, exhibiting induced resistance by unknown mechanisms. Therefore, the objective of this study was to identify the ultrastructural changes in viable cells of E. coli adapted to high concentrations (7000 ppm) of these antimicrobials, using transmission electron microscopy (TEM). After treatment with potassium sorbate, E. coli presented important morphological changes such as the separation of the cell membrane from the cytoplasm and cell wall, the appearance of a remarkable electronic light at the center of cells containing condensed deoxyribonucleic acid (DNA) molecules, as well as the appearance of small dense granules of electrons. Therefores, potassium sorbate induced more severe shape structural changes, presence of unusual structures and loss of integrity compared to viable cells adapted to sodium benzoate.
VL  - 4
IS  - 4
ER  -

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