The Dose Effect of Whey Protein on Glycemic Control in Adults with Insulin Resistance

Todd Castleberry; Christopher Irvine; Ryan Gordon; Matthew Brisebois; Sarah Deemer; Aubrien Henderson; Matthew Sokoloski; Vic Ben-Ezra

doi:doi:10.11648/j.ijfsb.20200504.12

The Dose Effect of Whey Protein on Glycemic Control in Adults with Insulin Resistance

Todd Castleberry, Christopher Irvine, Ryan Gordon, Matthew Brisebois, Sarah Deemer, Aubrien Henderson, Matthew Sokoloski, Vic Ben-Ezra

Published in International Journal of Food Science and Biotechnology (Volume 5, Issue 4)

Received: 9 October 2020 Accepted: 21 October 2020 Published: 30 October 2020

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Abstract

Whey protein coupled with a glucose challenge increases insulin secretion and may decrease glucose responses in people with pre-diabetes and type 2 diabetes. These responses may be attributed to whey protein’s effect on the incretins glucagon-like peptide-1 (GLP-1) and glucose dependent insulinotropic polypeptide (GIP). The purpose of this study was to examine the effect of various doses of whey protein on postprandial glycemic control and incretin responses. Participants with insulin resistance (n=9, mean ± SD; age: 64.3 + 8.1 yrs; BMI: 29.4 + 6.0 kg/m²; fasting plasma glucose: 6.9 + 1.2 mmol/l; HbA1c: 6.4 + 0.6%) completed three randomized treatments. Treatment 1 included 250 ml water + 20 g whey protein (T₁), and treatment 2 included 250 ml water + 30 g whey protein (T₂). The control treatment included 250 ml water (CON). Each treatment was followed by a 50 g oral glucose tolerance test. Incremental area under the curve (_iAUC) for insulin increased from CON to T₁ (P<0.01, 45.5%), CON to T₂ (P<0.01, 61.0%), and T₁ to T₂ (P<0.01, 28.5%), with a significant decrease in postprandial AUC for glucose with T₂ (P=0.04, -41.2%). Neither GIP nor GLP-1 _iAUC increased with T₁ or T₂ compared to CON. However, postprandial glucose _iAUC was significantly reduced for T₂ compared to CON (P=0.04, -41.2%). There was a dose effect of whey protein on plasma insulin with a significant decrease in postprandial glucose _iAUC following T₂. Thirty grams of a whey protein preload may be adequate to provide postprandial glycemic improvements in the disease management of type 2 diabetes or pre-diabetes

Published in	International Journal of Food Science and Biotechnology (Volume 5, Issue 4)
DOI	10.11648/j.ijfsb.20200504.12
Page(s)	52-57
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2020. Published by Science Publishing Group

Keywords

Diabetes, Pre-diabetes, GIP, GLP-1, Glucose, Glucagon

References

[1]	Rogowicz-Frontczak, A., Majchrzak, A., Zozulinka-Zoilkiewicz, D. (2017). Insulin resistance in endocrine disorders-treatment options. Endokrynol Pol; 68 (3): 334-342.
[2]	Olokoba, A. B., Obateru, O. A., Olokoba, L. B. (2012) Type 2 diabetes mellitus: A review of current trends. Oman Medical Journal Jul; 27 (4): 269-273.
[3]	Russell, S. (2013). Incretin-based therapies for type 2 diabetes mellitus: a review of direct comparisons of efficacy, safety and patient satisfaction. Int J Clin Pharm Apr; 35 (2): 159-172.
[4]	Holst, J., & Deacon, C. (2013). Is there a place for incretin therapies in obesity and prediabetes? Trends in Endocrinology and Metabolism, 24 (3), 145-152.
[5]	Bagger, J., Knop, F., Lund, A., Vestergaard, H., Holst, J., & Visboll, T. (2011). Impaired regulation of the incretin effect in patients with type 2 diabetes. Journal of Clinical Endocrinology and Metabolism, 3 (96), 737-745.
[6]	Allerton, D., Rumbold, P., West, D., & Stevenson, E. (2019). Effect of supplemental whey protein timing on postprandial glycaemia in centrally-obese males. British Journal of Nutrition, 121 (6), 637-646.
[7]	King, D. G., Walker, M., Campbell, M. D., Breen, L., Stevenson, E. J., & West, D. J. (2018). A small dose of whey protein co-ingested with mixed-macronutrient breakfast and lunch meals improved postprandial glycemia and suppresses appetite in men with type 2 diabetes: a randomized controlled trial. American Journal of Clinical Nutrition, 107 (4), 550-557.
[8]	Jakubowicz, D., Froy, O., Ahrén, B., Boaz, M., Landau, Z., Bar-Dayan, Y., Ganz, T., Barnea, M., & Wainstein, J. (2014). Incretin, insulinotropic and glucose-lowering effects of whey protein pre-load in type 2 diabetes: A randomised clinical trial. Diabetologia, 57 (9), 1807-11.
[9]	Akhavan, T., Luhovyy, B., Brown, P., Cho, C., & Anderson G. (2010). Effect of premeal consumption of whey protein and its hydrolysate on food intake and postmeal glycemia and insulin responses in young adults. American Journal of Clinical Nutrition, 91 (4), 966-75. doi: 10.3945/ajcn.2009.28406.
[10]	Ma, J., Jesudason, D. R., Stevens, J. E., Keogh, J. B., Jones K. L., Clifton, P. M., Horowitz, M., & Rayner, C. K. (2015). Sustained effects of a protein ‘preload’ on glycaemia and gastric emptying over 4 weeks in patients with type 2 diabetes: A randomized clinical trial. Diabetes Research Clinical Practice, 108 (2), e31-e34.
[11]	Petersen, B. L., Ward, L. S., Bastian, E. D., Jenkins, A. L., Campbell, J., & Vuksan, V. (2009). A whey protein supplement decreases post-prandial glycemia. Nutrition Journal, 16 (8), 47.
[12]	Akhavan, T., Luhovyy, B., Panahi, S., Kubant, R., Brown, P., & Anderson, G. (2014). Mechanism of action of pre-meal consumption of whey protein on glycemic control in young adults. Journal of Nutrition Biochemistry, 25 (1), 36-43. doi: 10.1016/j.jnutbio.2013.08.012.
[13]	American Diabetes Association. (2018). Classification and diagnosis of diabetes: Standards of medical care in diabetes-2018. Diabetes Care 41 (1): S13-S27.
[14]	Ma, J., Stevens, J. E., Cukier, K., Maddox, A. F., Wishart, J. M., Jones, K. L., Clifton, P. M., Horowitz, M., & Rayner, C. K. (2009). Effects of a protein preload on gastric emptying, glycemia, and gut hormones after a carbohydrate meal in diet-controlled type 2 diabetes. Diabetes Care, 32 (9), 1600-1602.
[15]	Hoefle, A., Bangert, A., Stamfort, A., Gedrich, K., Rist, M., Lee, Y., Skurk, T., & Daniel, H. (2015). Metabolic responses of healthy or prediabetic adults to bovine whey protein and sodium caseinate do not differ. Journal of Nutrition, 145 (3), 467-475.
[16]	Holst, J. J., Gromada, J. (2004). Role of incretin hormones in the regulation of insulin secretion in diabetic and nondiabetic humans. American Journal of Physiology, Endocrinology and Metabolism. 287 (2), E199-206.
[17]	Herchuelz, A., Lebrun, P., Boschero, A. C., Malaisse, W. J. (1984). Mechanism of arginine-stimulated Ca2+ influx into pancreatic B cell. American Journal of Physiology, 246 (1 Pt 1): E38-43.
[18]	Sener, A., Best, L. C., Yates, A. P., Kadiata, M. M., Olivares, E., Louchami, K., Jijakli, H., Ladriere, L., Malaisse, W. J. (2000). Stimulus-secretion coupling of arginine-induced insulin release: comparison between the cationic amino acid and its methyl ester. Endocrine, 13 (3), 329-340.
[19]	van Loon LJ, Saris WH, Verhagen H, Wagenmakers AJ. Plasma insulin responses after ingestion of different amino acid or protein mixtures with carbohydrate. Am J Clin Nutr. 2000 Jul; 72 (1): 96–105.
[20]	Kalogeropoulou D, Lafave L, Schweim K, Gannon MC, Nuttall FQ. Leucine, when ingested with glucose, synergistically stimulates insulin secretion and lowers blood glucose. Metab Clin Exp. 2008 Dec; 57 (12): 1747–52.
[21]	Smith, G. I., Yoshino, J., Stromsdorfer, K. L., Klein, S. J., Magkos, F., Reeds, D. N., Klein, S., Mittendorfer, B. (2015). Protein ingestion induces muscle insulin resistance independent of leucine-mediated mTOR activation. Diabetes 64 (5), 1555-1563.
[22]	Cherrington, A., Lacy, W., & Chiasson, J. (1978). Effect of glucagon on glucose production during insulin deficiency in the dog. Journal of Clinical Investigation, 62 (3), 664-677.
[23]	Kaneko, K., Shirotani, T., Araki, E., Matsumoto, K., Taguchi, T., Motoshima, H., Yoshizato, K., Kishikawa, H., Shichiri, M. (1999). Insulin inhibits glucagon secretion by the activation of PI3-kinase in In-R1-G9 cells. Diabetes Research in Clinical Practice, 442 (2), 83-92.
[24]	Seino, Y., Fukushima, M., & Yabe, D. (2010). GIP and GLP-1, the two incretin hormones: Similiarities and differences. Journal of Diabetes Investigation, 1 (1-2), 8-23.
[25]	Frid, A., Nilsson, M., Holst, J., Bjorck, I. (2005). Effect of whey on blood glucose and insulin responses to composite breakfast and lunch meals in type 2 diabetic subjects. American Journal of Clinical Nutrition, 82 (1), 69-75.

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APA Style

Todd Castleberry, Christopher Irvine, Ryan Gordon, Matthew Brisebois, Sarah Deemer, et al. (2020). The Dose Effect of Whey Protein on Glycemic Control in Adults with Insulin Resistance. International Journal of Food Science and Biotechnology, 5(4), 52-57. https://doi.org/10.11648/j.ijfsb.20200504.12

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ACS Style

Todd Castleberry; Christopher Irvine; Ryan Gordon; Matthew Brisebois; Sarah Deemer, et al. The Dose Effect of Whey Protein on Glycemic Control in Adults with Insulin Resistance. Int. J. Food Sci. Biotechnol. 2020, 5(4), 52-57. doi: 10.11648/j.ijfsb.20200504.12

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AMA Style

Todd Castleberry, Christopher Irvine, Ryan Gordon, Matthew Brisebois, Sarah Deemer, et al. The Dose Effect of Whey Protein on Glycemic Control in Adults with Insulin Resistance. Int J Food Sci Biotechnol. 2020;5(4):52-57. doi: 10.11648/j.ijfsb.20200504.12

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@article{10.11648/j.ijfsb.20200504.12,
  author = {Todd Castleberry and Christopher Irvine and Ryan Gordon and Matthew Brisebois and Sarah Deemer and Aubrien Henderson and Matthew Sokoloski and Vic Ben-Ezra},
  title = {The Dose Effect of Whey Protein on Glycemic Control in Adults with Insulin Resistance},
  journal = {International Journal of Food Science and Biotechnology},
  volume = {5},
  number = {4},
  pages = {52-57},
  doi = {10.11648/j.ijfsb.20200504.12},
  url = {https://doi.org/10.11648/j.ijfsb.20200504.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijfsb.20200504.12},
  abstract = {Whey protein coupled with a glucose challenge increases insulin secretion and may decrease glucose responses in people with pre-diabetes and type 2 diabetes. These responses may be attributed to whey protein’s effect on the incretins glucagon-like peptide-1 (GLP-1) and glucose dependent insulinotropic polypeptide (GIP). The purpose of this study was to examine the effect of various doses of whey protein on postprandial glycemic control and incretin responses. Participants with insulin resistance (n=9, mean ± SD; age: 64.3 + 8.1 yrs; BMI: 29.4 + 6.0 kg/m2; fasting plasma glucose: 6.9 + 1.2 mmol/l; HbA1c: 6.4 + 0.6%) completed three randomized treatments. Treatment 1 included 250 ml water + 20 g whey protein (T1), and treatment 2 included 250 ml water + 30 g whey protein (T2). The control treatment included 250 ml water (CON). Each treatment was followed by a 50 g oral glucose tolerance test. Incremental area under the curve (iAUC) for insulin increased from CON to T1 (P2 (P1 to T2 (P2 (P=0.04, -41.2%). Neither GIP nor GLP-1 iAUC increased with T1 or T2 compared to CON. However, postprandial glucose iAUC was significantly reduced for T2 compared to CON (P=0.04, -41.2%). There was a dose effect of whey protein on plasma insulin with a significant decrease in postprandial glucose iAUC following T2. Thirty grams of a whey protein preload may be adequate to provide postprandial glycemic improvements in the disease management of type 2 diabetes or pre-diabetes},
 year = {2020}
}

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TY  - JOUR
T1  - The Dose Effect of Whey Protein on Glycemic Control in Adults with Insulin Resistance
AU  - Todd Castleberry
AU  - Christopher Irvine
AU  - Ryan Gordon
AU  - Matthew Brisebois
AU  - Sarah Deemer
AU  - Aubrien Henderson
AU  - Matthew Sokoloski
AU  - Vic Ben-Ezra
Y1  - 2020/10/30
PY  - 2020
N1  - https://doi.org/10.11648/j.ijfsb.20200504.12
DO  - 10.11648/j.ijfsb.20200504.12
T2  - International Journal of Food Science and Biotechnology
JF  - International Journal of Food Science and Biotechnology
JO  - International Journal of Food Science and Biotechnology
SP  - 52
EP  - 57
PB  - Science Publishing Group
SN  - 2578-9643
UR  - https://doi.org/10.11648/j.ijfsb.20200504.12
AB  - Whey protein coupled with a glucose challenge increases insulin secretion and may decrease glucose responses in people with pre-diabetes and type 2 diabetes. These responses may be attributed to whey protein’s effect on the incretins glucagon-like peptide-1 (GLP-1) and glucose dependent insulinotropic polypeptide (GIP). The purpose of this study was to examine the effect of various doses of whey protein on postprandial glycemic control and incretin responses. Participants with insulin resistance (n=9, mean ± SD; age: 64.3 + 8.1 yrs; BMI: 29.4 + 6.0 kg/m2; fasting plasma glucose: 6.9 + 1.2 mmol/l; HbA1c: 6.4 + 0.6%) completed three randomized treatments. Treatment 1 included 250 ml water + 20 g whey protein (T1), and treatment 2 included 250 ml water + 30 g whey protein (T2). The control treatment included 250 ml water (CON). Each treatment was followed by a 50 g oral glucose tolerance test. Incremental area under the curve (iAUC) for insulin increased from CON to T1 (P2 (P1 to T2 (P2 (P=0.04, -41.2%). Neither GIP nor GLP-1 iAUC increased with T1 or T2 compared to CON. However, postprandial glucose iAUC was significantly reduced for T2 compared to CON (P=0.04, -41.2%). There was a dose effect of whey protein on plasma insulin with a significant decrease in postprandial glucose iAUC following T2. Thirty grams of a whey protein preload may be adequate to provide postprandial glycemic improvements in the disease management of type 2 diabetes or pre-diabetes
VL  - 5
IS  - 4
ER  -

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Author Information

Todd Castleberry

School of Health Promotion & Kinesiology, Texas Woman’s University, Denton, USA
Christopher Irvine

School of Health Promotion & Kinesiology, Texas Woman’s University, Denton, USA
Ryan Gordon

School of Health Promotion & Kinesiology, Texas Woman’s University, Denton, USA
Matthew Brisebois

School of Health Promotion & Kinesiology, Texas Woman’s University, Denton, USA
Sarah Deemer

School of Health Promotion & Kinesiology, Texas Woman’s University, Denton, USA
Aubrien Henderson

School of Health Promotion & Kinesiology, Texas Woman’s University, Denton, USA
Matthew Sokoloski

School of Health Promotion & Kinesiology, Texas Woman’s University, Denton, USA
Vic Ben-Ezra

School of Health Promotion & Kinesiology, Texas Woman’s University, Denton, USA

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APA Style

Todd Castleberry, Christopher Irvine, Ryan Gordon, Matthew Brisebois, Sarah Deemer, et al. (2020). The Dose Effect of Whey Protein on Glycemic Control in Adults with Insulin Resistance. International Journal of Food Science and Biotechnology, 5(4), 52-57. https://doi.org/10.11648/j.ijfsb.20200504.12

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ACS Style

Todd Castleberry; Christopher Irvine; Ryan Gordon; Matthew Brisebois; Sarah Deemer, et al. The Dose Effect of Whey Protein on Glycemic Control in Adults with Insulin Resistance. Int. J. Food Sci. Biotechnol. 2020, 5(4), 52-57. doi: 10.11648/j.ijfsb.20200504.12

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AMA Style

Todd Castleberry, Christopher Irvine, Ryan Gordon, Matthew Brisebois, Sarah Deemer, et al. The Dose Effect of Whey Protein on Glycemic Control in Adults with Insulin Resistance. Int J Food Sci Biotechnol. 2020;5(4):52-57. doi: 10.11648/j.ijfsb.20200504.12

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@article{10.11648/j.ijfsb.20200504.12,
  author = {Todd Castleberry and Christopher Irvine and Ryan Gordon and Matthew Brisebois and Sarah Deemer and Aubrien Henderson and Matthew Sokoloski and Vic Ben-Ezra},
  title = {The Dose Effect of Whey Protein on Glycemic Control in Adults with Insulin Resistance},
  journal = {International Journal of Food Science and Biotechnology},
  volume = {5},
  number = {4},
  pages = {52-57},
  doi = {10.11648/j.ijfsb.20200504.12},
  url = {https://doi.org/10.11648/j.ijfsb.20200504.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijfsb.20200504.12},
  abstract = {Whey protein coupled with a glucose challenge increases insulin secretion and may decrease glucose responses in people with pre-diabetes and type 2 diabetes. These responses may be attributed to whey protein’s effect on the incretins glucagon-like peptide-1 (GLP-1) and glucose dependent insulinotropic polypeptide (GIP). The purpose of this study was to examine the effect of various doses of whey protein on postprandial glycemic control and incretin responses. Participants with insulin resistance (n=9, mean ± SD; age: 64.3 + 8.1 yrs; BMI: 29.4 + 6.0 kg/m2; fasting plasma glucose: 6.9 + 1.2 mmol/l; HbA1c: 6.4 + 0.6%) completed three randomized treatments. Treatment 1 included 250 ml water + 20 g whey protein (T1), and treatment 2 included 250 ml water + 30 g whey protein (T2). The control treatment included 250 ml water (CON). Each treatment was followed by a 50 g oral glucose tolerance test. Incremental area under the curve (iAUC) for insulin increased from CON to T1 (P2 (P1 to T2 (P2 (P=0.04, -41.2%). Neither GIP nor GLP-1 iAUC increased with T1 or T2 compared to CON. However, postprandial glucose iAUC was significantly reduced for T2 compared to CON (P=0.04, -41.2%). There was a dose effect of whey protein on plasma insulin with a significant decrease in postprandial glucose iAUC following T2. Thirty grams of a whey protein preload may be adequate to provide postprandial glycemic improvements in the disease management of type 2 diabetes or pre-diabetes},
 year = {2020}
}

Copy | Download

TY  - JOUR
T1  - The Dose Effect of Whey Protein on Glycemic Control in Adults with Insulin Resistance
AU  - Todd Castleberry
AU  - Christopher Irvine
AU  - Ryan Gordon
AU  - Matthew Brisebois
AU  - Sarah Deemer
AU  - Aubrien Henderson
AU  - Matthew Sokoloski
AU  - Vic Ben-Ezra
Y1  - 2020/10/30
PY  - 2020
N1  - https://doi.org/10.11648/j.ijfsb.20200504.12
DO  - 10.11648/j.ijfsb.20200504.12
T2  - International Journal of Food Science and Biotechnology
JF  - International Journal of Food Science and Biotechnology
JO  - International Journal of Food Science and Biotechnology
SP  - 52
EP  - 57
PB  - Science Publishing Group
SN  - 2578-9643
UR  - https://doi.org/10.11648/j.ijfsb.20200504.12
AB  - Whey protein coupled with a glucose challenge increases insulin secretion and may decrease glucose responses in people with pre-diabetes and type 2 diabetes. These responses may be attributed to whey protein’s effect on the incretins glucagon-like peptide-1 (GLP-1) and glucose dependent insulinotropic polypeptide (GIP). The purpose of this study was to examine the effect of various doses of whey protein on postprandial glycemic control and incretin responses. Participants with insulin resistance (n=9, mean ± SD; age: 64.3 + 8.1 yrs; BMI: 29.4 + 6.0 kg/m2; fasting plasma glucose: 6.9 + 1.2 mmol/l; HbA1c: 6.4 + 0.6%) completed three randomized treatments. Treatment 1 included 250 ml water + 20 g whey protein (T1), and treatment 2 included 250 ml water + 30 g whey protein (T2). The control treatment included 250 ml water (CON). Each treatment was followed by a 50 g oral glucose tolerance test. Incremental area under the curve (iAUC) for insulin increased from CON to T1 (P2 (P1 to T2 (P2 (P=0.04, -41.2%). Neither GIP nor GLP-1 iAUC increased with T1 or T2 compared to CON. However, postprandial glucose iAUC was significantly reduced for T2 compared to CON (P=0.04, -41.2%). There was a dose effect of whey protein on plasma insulin with a significant decrease in postprandial glucose iAUC following T2. Thirty grams of a whey protein preload may be adequate to provide postprandial glycemic improvements in the disease management of type 2 diabetes or pre-diabetes
VL  - 5
IS  - 4
ER  -

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