Comparative Study of Anti-Anemic Effect of Some Natural Food Supplements on Rats

Safaa Ahmed Salem; Amany Mohammed Gad; Abdellatif Anaam Kamal

doi:doi:10.11648/j.ijfsb.20210602.11

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Comparative Study of Anti-Anemic Effect of Some Natural Food Supplements on Rats

Safaa Ahmed Salem, Amany Mohammed Gad, Abdellatif Anaam Kamal

Published in International Journal of Food Science and Biotechnology (Volume 6, Issue 2)

Received: 2 March 2021 Accepted: 24 March 2021 Published: 7 April 2021

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Abstract

In this study beetroot and eggplant were used as natural source for production of anti-anemic functional food for anemic patients and to prevent it during disease or chemotherapy treatment. Anemic model was induced in albino female rats by daily injection with phenylhydrazin (5mg/kg b.w) for three successive days, then biochemical, hematological and histological parameters were determined including; iron, ferritin, Ascorbic acid, Hepicidin, Hemoglobin and Complete blood count (men corpuscle volume, men corpuscle hemoglobin, haematocriet, red blood cells and white blood cells). Negative control group of normal rats, and positive control group of anemic rats each of six rats were fed on basal diet. Also 24 anemic rats were divided into 4 groups each of 6 rats which were fed on basal diet containing either eggplant or beetroot in ratios of 100, 300g/kg, in addition to 500mg ascorbic acid and 2mg folic acid/ kg diet for all groups for 30 days. feeding period was 30 days. At the end of experiment the above biochemical and haematological parameters were evaluated and the liver and spleen were histopathological examined. Decreases in hemoglobin, men corpuscle volume, haematocriet, red blood cells, men corpuscle hemoglobin, iron, ascorbic acid and ferritin, also increases in white blood cells and hepcidin were observed in anemic model rats. At the end of feeding period with the previous supplemented tested basal diet, increases in all aforementioned parameters, except white blood cells and hepcidin which showed decreases compared to anemic positive group were found.

Published in	International Journal of Food Science and Biotechnology (Volume 6, Issue 2)
DOI	10.11648/j.ijfsb.20210602.11
Page(s)	21-29
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2021. Published by Science Publishing Group

Keywords

Anemic, Beetroot, Chemical Properties, Eggplant, Hematological, Histopathologjcal

References

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APA Style

Safaa Ahmed Salem, Amany Mohammed Gad, Abdellatif Anaam Kamal. (2021). Comparative Study of Anti-Anemic Effect of Some Natural Food Supplements on Rats. International Journal of Food Science and Biotechnology, 6(2), 21-29. https://doi.org/10.11648/j.ijfsb.20210602.11

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ACS Style

Safaa Ahmed Salem; Amany Mohammed Gad; Abdellatif Anaam Kamal. Comparative Study of Anti-Anemic Effect of Some Natural Food Supplements on Rats. Int. J. Food Sci. Biotechnol. 2021, 6(2), 21-29. doi: 10.11648/j.ijfsb.20210602.11

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AMA Style

Safaa Ahmed Salem, Amany Mohammed Gad, Abdellatif Anaam Kamal. Comparative Study of Anti-Anemic Effect of Some Natural Food Supplements on Rats. Int J Food Sci Biotechnol. 2021;6(2):21-29. doi: 10.11648/j.ijfsb.20210602.11

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@article{10.11648/j.ijfsb.20210602.11,
  author = {Safaa Ahmed Salem and Amany Mohammed Gad and Abdellatif Anaam Kamal},
  title = {Comparative Study of Anti-Anemic Effect of Some Natural Food Supplements on Rats},
  journal = {International Journal of Food Science and Biotechnology},
  volume = {6},
  number = {2},
  pages = {21-29},
  doi = {10.11648/j.ijfsb.20210602.11},
  url = {https://doi.org/10.11648/j.ijfsb.20210602.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijfsb.20210602.11},
  abstract = {In this study beetroot and eggplant were used as natural source for production of anti-anemic functional food for anemic patients and to prevent it during disease or chemotherapy treatment. Anemic model was induced in albino female rats by daily injection with phenylhydrazin (5mg/kg b.w) for three successive days, then biochemical, hematological and histological parameters were determined including; iron, ferritin, Ascorbic acid, Hepicidin, Hemoglobin and Complete blood count (men corpuscle volume, men corpuscle hemoglobin, haematocriet, red blood cells and white blood cells). Negative control group of normal rats, and positive control group of anemic rats each of six rats were fed on basal diet. Also 24 anemic rats were divided into 4 groups each of 6 rats which were fed on basal diet containing either eggplant or beetroot in ratios of 100, 300g/kg, in addition to 500mg ascorbic acid and 2mg folic acid/ kg diet for all groups for 30 days. feeding period was 30 days. At the end of experiment the above biochemical and haematological parameters were evaluated and the liver and spleen were histopathological examined. Decreases in hemoglobin, men corpuscle volume, haematocriet, red blood cells, men corpuscle hemoglobin, iron, ascorbic acid and ferritin, also increases in white blood cells and hepcidin were observed in anemic model rats. At the end of feeding period with the previous supplemented tested basal diet, increases in all aforementioned parameters, except white blood cells and hepcidin which showed decreases compared to anemic positive group were found.},
 year = {2021}
}

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TY  - JOUR
T1  - Comparative Study of Anti-Anemic Effect of Some Natural Food Supplements on Rats
AU  - Safaa Ahmed Salem
AU  - Amany Mohammed Gad
AU  - Abdellatif Anaam Kamal
Y1  - 2021/04/07
PY  - 2021
N1  - https://doi.org/10.11648/j.ijfsb.20210602.11
DO  - 10.11648/j.ijfsb.20210602.11
T2  - International Journal of Food Science and Biotechnology
JF  - International Journal of Food Science and Biotechnology
JO  - International Journal of Food Science and Biotechnology
SP  - 21
EP  - 29
PB  - Science Publishing Group
SN  - 2578-9643
UR  - https://doi.org/10.11648/j.ijfsb.20210602.11
AB  - In this study beetroot and eggplant were used as natural source for production of anti-anemic functional food for anemic patients and to prevent it during disease or chemotherapy treatment. Anemic model was induced in albino female rats by daily injection with phenylhydrazin (5mg/kg b.w) for three successive days, then biochemical, hematological and histological parameters were determined including; iron, ferritin, Ascorbic acid, Hepicidin, Hemoglobin and Complete blood count (men corpuscle volume, men corpuscle hemoglobin, haematocriet, red blood cells and white blood cells). Negative control group of normal rats, and positive control group of anemic rats each of six rats were fed on basal diet. Also 24 anemic rats were divided into 4 groups each of 6 rats which were fed on basal diet containing either eggplant or beetroot in ratios of 100, 300g/kg, in addition to 500mg ascorbic acid and 2mg folic acid/ kg diet for all groups for 30 days. feeding period was 30 days. At the end of experiment the above biochemical and haematological parameters were evaluated and the liver and spleen were histopathological examined. Decreases in hemoglobin, men corpuscle volume, haematocriet, red blood cells, men corpuscle hemoglobin, iron, ascorbic acid and ferritin, also increases in white blood cells and hepcidin were observed in anemic model rats. At the end of feeding period with the previous supplemented tested basal diet, increases in all aforementioned parameters, except white blood cells and hepcidin which showed decreases compared to anemic positive group were found.
VL  - 6
IS  - 2
ER  -

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Author Information

Safaa Ahmed Salem

Department of Medicinal Food, National Organization for Drug Control and Research (NODCAR), Giza, Egypt
Amany Mohammed Gad

Department Pharmacology, National Organization for Drug Control and Research (NODCAR), Giza, Egypt
Abdellatif Anaam Kamal

Department of Medicinal Food, National Organization for Drug Control and Research (NODCAR), Giza, Egypt

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APA Style

Safaa Ahmed Salem, Amany Mohammed Gad, Abdellatif Anaam Kamal. (2021). Comparative Study of Anti-Anemic Effect of Some Natural Food Supplements on Rats. International Journal of Food Science and Biotechnology, 6(2), 21-29. https://doi.org/10.11648/j.ijfsb.20210602.11

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ACS Style

Safaa Ahmed Salem; Amany Mohammed Gad; Abdellatif Anaam Kamal. Comparative Study of Anti-Anemic Effect of Some Natural Food Supplements on Rats. Int. J. Food Sci. Biotechnol. 2021, 6(2), 21-29. doi: 10.11648/j.ijfsb.20210602.11

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AMA Style

Safaa Ahmed Salem, Amany Mohammed Gad, Abdellatif Anaam Kamal. Comparative Study of Anti-Anemic Effect of Some Natural Food Supplements on Rats. Int J Food Sci Biotechnol. 2021;6(2):21-29. doi: 10.11648/j.ijfsb.20210602.11

Copy | Download

@article{10.11648/j.ijfsb.20210602.11,
  author = {Safaa Ahmed Salem and Amany Mohammed Gad and Abdellatif Anaam Kamal},
  title = {Comparative Study of Anti-Anemic Effect of Some Natural Food Supplements on Rats},
  journal = {International Journal of Food Science and Biotechnology},
  volume = {6},
  number = {2},
  pages = {21-29},
  doi = {10.11648/j.ijfsb.20210602.11},
  url = {https://doi.org/10.11648/j.ijfsb.20210602.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijfsb.20210602.11},
  abstract = {In this study beetroot and eggplant were used as natural source for production of anti-anemic functional food for anemic patients and to prevent it during disease or chemotherapy treatment. Anemic model was induced in albino female rats by daily injection with phenylhydrazin (5mg/kg b.w) for three successive days, then biochemical, hematological and histological parameters were determined including; iron, ferritin, Ascorbic acid, Hepicidin, Hemoglobin and Complete blood count (men corpuscle volume, men corpuscle hemoglobin, haematocriet, red blood cells and white blood cells). Negative control group of normal rats, and positive control group of anemic rats each of six rats were fed on basal diet. Also 24 anemic rats were divided into 4 groups each of 6 rats which were fed on basal diet containing either eggplant or beetroot in ratios of 100, 300g/kg, in addition to 500mg ascorbic acid and 2mg folic acid/ kg diet for all groups for 30 days. feeding period was 30 days. At the end of experiment the above biochemical and haematological parameters were evaluated and the liver and spleen were histopathological examined. Decreases in hemoglobin, men corpuscle volume, haematocriet, red blood cells, men corpuscle hemoglobin, iron, ascorbic acid and ferritin, also increases in white blood cells and hepcidin were observed in anemic model rats. At the end of feeding period with the previous supplemented tested basal diet, increases in all aforementioned parameters, except white blood cells and hepcidin which showed decreases compared to anemic positive group were found.},
 year = {2021}
}

Copy | Download

TY  - JOUR
T1  - Comparative Study of Anti-Anemic Effect of Some Natural Food Supplements on Rats
AU  - Safaa Ahmed Salem
AU  - Amany Mohammed Gad
AU  - Abdellatif Anaam Kamal
Y1  - 2021/04/07
PY  - 2021
N1  - https://doi.org/10.11648/j.ijfsb.20210602.11
DO  - 10.11648/j.ijfsb.20210602.11
T2  - International Journal of Food Science and Biotechnology
JF  - International Journal of Food Science and Biotechnology
JO  - International Journal of Food Science and Biotechnology
SP  - 21
EP  - 29
PB  - Science Publishing Group
SN  - 2578-9643
UR  - https://doi.org/10.11648/j.ijfsb.20210602.11
AB  - In this study beetroot and eggplant were used as natural source for production of anti-anemic functional food for anemic patients and to prevent it during disease or chemotherapy treatment. Anemic model was induced in albino female rats by daily injection with phenylhydrazin (5mg/kg b.w) for three successive days, then biochemical, hematological and histological parameters were determined including; iron, ferritin, Ascorbic acid, Hepicidin, Hemoglobin and Complete blood count (men corpuscle volume, men corpuscle hemoglobin, haematocriet, red blood cells and white blood cells). Negative control group of normal rats, and positive control group of anemic rats each of six rats were fed on basal diet. Also 24 anemic rats were divided into 4 groups each of 6 rats which were fed on basal diet containing either eggplant or beetroot in ratios of 100, 300g/kg, in addition to 500mg ascorbic acid and 2mg folic acid/ kg diet for all groups for 30 days. feeding period was 30 days. At the end of experiment the above biochemical and haematological parameters were evaluated and the liver and spleen were histopathological examined. Decreases in hemoglobin, men corpuscle volume, haematocriet, red blood cells, men corpuscle hemoglobin, iron, ascorbic acid and ferritin, also increases in white blood cells and hepcidin were observed in anemic model rats. At the end of feeding period with the previous supplemented tested basal diet, increases in all aforementioned parameters, except white blood cells and hepcidin which showed decreases compared to anemic positive group were found.
VL  - 6
IS  - 2
ER  -

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