<i>IDH1</i> Mutation in Gliomas in Baghdad by Immunohistochemical Study

Zahraa Marwan Shaban; Salim Rasheed Al-Aubaidy; Ameer Dhahir Hameedi

doi:doi:10.11648/j.ijgg.20180601.11

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IDH1 Mutation in Gliomas in Baghdad by Immunohistochemical Study

Zahraa Marwan Shaban, Salim Rasheed Al-Aubaidy, Ameer Dhahir Hameedi

Published in International Journal of Genetics and Genomics (Volume 6, Issue 1)

Received: 7 November 2017 Accepted: 20 November 2017 Published: 11 January 2018

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Abstract

IDH1 (isocitrate dehydrogenase 1) mutation might be encounter in the low-grade glioma occurs in early stages of development and directs the progression of the tumor to a higher grade. Aim of the study was to assess the frequency of IDH1 mutation in Iraqi patients with gliomas by immunohistochemical study, to correlate its immunoreactivity with some clinicopathological parameters. The study did on formalin fixed, paraffin embedded tumor tissue from 66 patients with different grades of intracranial gliomas of both gender and all age groups in the Baghdad city were collected in this retrospective and prospective randomized study. Ten normal brain tissue samples in form of paraffin blocks took from forensic medicine unit. New technique used, which is manual tissue microarray Immunohistochemical detection of IDH1 antibodies did by Dako autostainer link 48. Positive cytoplasmic IDH1 staining was found in 38 (57.6%) of cases of glioma. In adult gliomas, secondary glioblastoma multiforme, low grade astrocytoma and oligodendroglial tumors had the greatest values of IDH1 positivity (87%, 80% and 72.72% respectively) followed by anaplastic astrocytoma (42%), then primary glioblastoma multiforme (26%). Males and females expressed the IDH1 equally. The conclusions from the work were IDH1 mutation commonly existed in adult gliomas, low-grade gliomas and secondary glioblastoma, it had no role in pediatric gliomas, and it could be a diagnostic and prognostic marker.

Published in	International Journal of Genetics and Genomics (Volume 6, Issue 1)
DOI	10.11648/j.ijgg.20180601.11
Page(s)	1-7
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2018. Published by Science Publishing Group

Keywords

IDH1, Adults, Pediatric, Manual Tissue Microarray, Glioma, Immunohistochemical Study

References

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APA Style

Zahraa Marwan Shaban, Salim Rasheed Al-Aubaidy, Ameer Dhahir Hameedi. (2018). IDH1 Mutation in Gliomas in Baghdad by Immunohistochemical Study. International Journal of Genetics and Genomics, 6(1), 1-7. https://doi.org/10.11648/j.ijgg.20180601.11

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ACS Style

Zahraa Marwan Shaban; Salim Rasheed Al-Aubaidy; Ameer Dhahir Hameedi. IDH1 Mutation in Gliomas in Baghdad by Immunohistochemical Study. Int. J. Genet. Genomics 2018, 6(1), 1-7. doi: 10.11648/j.ijgg.20180601.11

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AMA Style

Zahraa Marwan Shaban, Salim Rasheed Al-Aubaidy, Ameer Dhahir Hameedi. IDH1 Mutation in Gliomas in Baghdad by Immunohistochemical Study. Int J Genet Genomics. 2018;6(1):1-7. doi: 10.11648/j.ijgg.20180601.11

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@article{10.11648/j.ijgg.20180601.11,
  author = {Zahraa Marwan Shaban and Salim Rasheed Al-Aubaidy and Ameer Dhahir Hameedi},
  title = {IDH1 Mutation in Gliomas in Baghdad by Immunohistochemical Study},
  journal = {International Journal of Genetics and Genomics},
  volume = {6},
  number = {1},
  pages = {1-7},
  doi = {10.11648/j.ijgg.20180601.11},
  url = {https://doi.org/10.11648/j.ijgg.20180601.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijgg.20180601.11},
  abstract = {IDH1 (isocitrate dehydrogenase 1) mutation might be encounter in the low-grade glioma occurs in early stages of development and directs the progression of the tumor to a higher grade. Aim of the study was to assess the frequency of IDH1 mutation in Iraqi patients with gliomas by immunohistochemical study, to correlate its immunoreactivity with some clinicopathological parameters. The study did on formalin fixed, paraffin embedded tumor tissue from 66 patients with different grades of intracranial gliomas of both gender and all age groups in the Baghdad city were collected in this retrospective and prospective randomized study. Ten normal brain tissue samples in form of paraffin blocks took from forensic medicine unit. New technique used, which is manual tissue microarray Immunohistochemical detection of IDH1 antibodies did by Dako autostainer link 48. Positive cytoplasmic IDH1 staining was found in 38 (57.6%) of cases of glioma. In adult gliomas, secondary glioblastoma multiforme, low grade astrocytoma and oligodendroglial tumors had the greatest values of IDH1 positivity (87%, 80% and 72.72% respectively) followed by anaplastic astrocytoma (42%), then primary glioblastoma multiforme (26%). Males and females expressed the IDH1 equally. The conclusions from the work were IDH1 mutation commonly existed in adult gliomas, low-grade gliomas and secondary glioblastoma, it had no role in pediatric gliomas, and it could be a diagnostic and prognostic marker.},
 year = {2018}
}

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TY  - JOUR
T1  - IDH1 Mutation in Gliomas in Baghdad by Immunohistochemical Study
AU  - Zahraa Marwan Shaban
AU  - Salim Rasheed Al-Aubaidy
AU  - Ameer Dhahir Hameedi
Y1  - 2018/01/11
PY  - 2018
N1  - https://doi.org/10.11648/j.ijgg.20180601.11
DO  - 10.11648/j.ijgg.20180601.11
T2  - International Journal of Genetics and Genomics
JF  - International Journal of Genetics and Genomics
JO  - International Journal of Genetics and Genomics
SP  - 1
EP  - 7
PB  - Science Publishing Group
SN  - 2376-7359
UR  - https://doi.org/10.11648/j.ijgg.20180601.11
AB  - IDH1 (isocitrate dehydrogenase 1) mutation might be encounter in the low-grade glioma occurs in early stages of development and directs the progression of the tumor to a higher grade. Aim of the study was to assess the frequency of IDH1 mutation in Iraqi patients with gliomas by immunohistochemical study, to correlate its immunoreactivity with some clinicopathological parameters. The study did on formalin fixed, paraffin embedded tumor tissue from 66 patients with different grades of intracranial gliomas of both gender and all age groups in the Baghdad city were collected in this retrospective and prospective randomized study. Ten normal brain tissue samples in form of paraffin blocks took from forensic medicine unit. New technique used, which is manual tissue microarray Immunohistochemical detection of IDH1 antibodies did by Dako autostainer link 48. Positive cytoplasmic IDH1 staining was found in 38 (57.6%) of cases of glioma. In adult gliomas, secondary glioblastoma multiforme, low grade astrocytoma and oligodendroglial tumors had the greatest values of IDH1 positivity (87%, 80% and 72.72% respectively) followed by anaplastic astrocytoma (42%), then primary glioblastoma multiforme (26%). Males and females expressed the IDH1 equally. The conclusions from the work were IDH1 mutation commonly existed in adult gliomas, low-grade gliomas and secondary glioblastoma, it had no role in pediatric gliomas, and it could be a diagnostic and prognostic marker.
VL  - 6
IS  - 1
ER  -

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Author Information

Zahraa Marwan Shaban

Pathology Department and Forensic Medicine, College of Medicine, University of Mosul, Mosul, Iraq
Salim Rasheed Al-Aubaidy

Pathology Department and Forensic Medicine, College of Medicine, University of Baghdad, Baghdad, Iraq
Ameer Dhahir Hameedi

Pathology Department and Forensic Medicine, College of Medicine, University of Baghdad, Baghdad, Iraq

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APA Style

Zahraa Marwan Shaban, Salim Rasheed Al-Aubaidy, Ameer Dhahir Hameedi. (2018). IDH1 Mutation in Gliomas in Baghdad by Immunohistochemical Study. International Journal of Genetics and Genomics, 6(1), 1-7. https://doi.org/10.11648/j.ijgg.20180601.11

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ACS Style

Zahraa Marwan Shaban; Salim Rasheed Al-Aubaidy; Ameer Dhahir Hameedi. IDH1 Mutation in Gliomas in Baghdad by Immunohistochemical Study. Int. J. Genet. Genomics 2018, 6(1), 1-7. doi: 10.11648/j.ijgg.20180601.11

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AMA Style

Zahraa Marwan Shaban, Salim Rasheed Al-Aubaidy, Ameer Dhahir Hameedi. IDH1 Mutation in Gliomas in Baghdad by Immunohistochemical Study. Int J Genet Genomics. 2018;6(1):1-7. doi: 10.11648/j.ijgg.20180601.11

Copy | Download

@article{10.11648/j.ijgg.20180601.11,
  author = {Zahraa Marwan Shaban and Salim Rasheed Al-Aubaidy and Ameer Dhahir Hameedi},
  title = {IDH1 Mutation in Gliomas in Baghdad by Immunohistochemical Study},
  journal = {International Journal of Genetics and Genomics},
  volume = {6},
  number = {1},
  pages = {1-7},
  doi = {10.11648/j.ijgg.20180601.11},
  url = {https://doi.org/10.11648/j.ijgg.20180601.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijgg.20180601.11},
  abstract = {IDH1 (isocitrate dehydrogenase 1) mutation might be encounter in the low-grade glioma occurs in early stages of development and directs the progression of the tumor to a higher grade. Aim of the study was to assess the frequency of IDH1 mutation in Iraqi patients with gliomas by immunohistochemical study, to correlate its immunoreactivity with some clinicopathological parameters. The study did on formalin fixed, paraffin embedded tumor tissue from 66 patients with different grades of intracranial gliomas of both gender and all age groups in the Baghdad city were collected in this retrospective and prospective randomized study. Ten normal brain tissue samples in form of paraffin blocks took from forensic medicine unit. New technique used, which is manual tissue microarray Immunohistochemical detection of IDH1 antibodies did by Dako autostainer link 48. Positive cytoplasmic IDH1 staining was found in 38 (57.6%) of cases of glioma. In adult gliomas, secondary glioblastoma multiforme, low grade astrocytoma and oligodendroglial tumors had the greatest values of IDH1 positivity (87%, 80% and 72.72% respectively) followed by anaplastic astrocytoma (42%), then primary glioblastoma multiforme (26%). Males and females expressed the IDH1 equally. The conclusions from the work were IDH1 mutation commonly existed in adult gliomas, low-grade gliomas and secondary glioblastoma, it had no role in pediatric gliomas, and it could be a diagnostic and prognostic marker.},
 year = {2018}
}

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TY  - JOUR
T1  - IDH1 Mutation in Gliomas in Baghdad by Immunohistochemical Study
AU  - Zahraa Marwan Shaban
AU  - Salim Rasheed Al-Aubaidy
AU  - Ameer Dhahir Hameedi
Y1  - 2018/01/11
PY  - 2018
N1  - https://doi.org/10.11648/j.ijgg.20180601.11
DO  - 10.11648/j.ijgg.20180601.11
T2  - International Journal of Genetics and Genomics
JF  - International Journal of Genetics and Genomics
JO  - International Journal of Genetics and Genomics
SP  - 1
EP  - 7
PB  - Science Publishing Group
SN  - 2376-7359
UR  - https://doi.org/10.11648/j.ijgg.20180601.11
AB  - IDH1 (isocitrate dehydrogenase 1) mutation might be encounter in the low-grade glioma occurs in early stages of development and directs the progression of the tumor to a higher grade. Aim of the study was to assess the frequency of IDH1 mutation in Iraqi patients with gliomas by immunohistochemical study, to correlate its immunoreactivity with some clinicopathological parameters. The study did on formalin fixed, paraffin embedded tumor tissue from 66 patients with different grades of intracranial gliomas of both gender and all age groups in the Baghdad city were collected in this retrospective and prospective randomized study. Ten normal brain tissue samples in form of paraffin blocks took from forensic medicine unit. New technique used, which is manual tissue microarray Immunohistochemical detection of IDH1 antibodies did by Dako autostainer link 48. Positive cytoplasmic IDH1 staining was found in 38 (57.6%) of cases of glioma. In adult gliomas, secondary glioblastoma multiforme, low grade astrocytoma and oligodendroglial tumors had the greatest values of IDH1 positivity (87%, 80% and 72.72% respectively) followed by anaplastic astrocytoma (42%), then primary glioblastoma multiforme (26%). Males and females expressed the IDH1 equally. The conclusions from the work were IDH1 mutation commonly existed in adult gliomas, low-grade gliomas and secondary glioblastoma, it had no role in pediatric gliomas, and it could be a diagnostic and prognostic marker.
VL  - 6
IS  - 1
ER  -

Copy | Download