Nanoparticles Induce Oxidative Stress in HT-29 Colon Adenocarcinoma Cell Line After 24 and 48 Hour Exposure

Metin Budak

doi:doi:10.11648/j.ijgg.20190704.14

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Nanoparticles Induce Oxidative Stress in HT-29 Colon Adenocarcinoma Cell Line After 24 and 48 Hour Exposure

Metin Budak

Published in International Journal of Genetics and Genomics (Volume 7, Issue 4)

Received: 31 August 2019 Accepted: 18 October 2019 Published: 26 October 2019

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Abstract

Nanoparticle research is currently an area of intense scientific research, due to a wide variety of potential applications in biomedical, optical, and electronic fields. Nanoparticles are of great scientific interest as they are effectively a bridge between bulk materials and atomic or molecular structures. Superoxide dismutase (SOD), Glutamine synthetase (GS), Catalase (CAT) are some of the defense mechanisms against cellular oxidative stress, especially against free oxygen radicals. In this study, we aimed to investigate that Ag, SiO₂ and ZnO nanoparticles affect cancer cell lines (HT-29) and relationship between SOD, GS and CAT. We investigated that alterations in gene expressions of SOD, GS and CAT caused by exposure to nanoparticles in HT-29 cells. The difference between the Ct values (ΔCt) of the gene of interest was calculated for each experimental sample. As a result of Ag, SiO₂ and ZnO nanoparticle application, there was a 2-fold increase in SOD and CAT expression in the first 24 hours compared to control. As a result of 48 hours of application, it was observed that Ag nanoparticles caused 4-fold increase in SOD and 6-fold statistically significant increase in CAT and GS expression of SiO₂ nanoparticles. Consequently, after 48 hours of nanoparticle application, SiO₂, CAT and GS expression were more effective than 24-hour application. Our results suggest that nanoparticles may cause increased oxidative stress in colon cells and may have therapeutic properties by affecting cancer cells in these aspects.

Published in	International Journal of Genetics and Genomics (Volume 7, Issue 4)
DOI	10.11648/j.ijgg.20190704.14
Page(s)	110-114
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2019. Published by Science Publishing Group

Keywords

Colon Adenocarcinoma, Nanoparticles, Oxidative Stress, SOD, GS, CAT

References

[1]	R. G. Haverkamp, A. T. Marshall, D. van Agterveld, (2007). Pick your carats: nanoparticles of gold–silver–copper alloy produced in vivo. Journal of Nanoparticle Research.; 9 (4): 697-700.
[2]	J. S. Han, J. H. Lee, J. Y. Kong, J. Kim, S. S. Choe, et al. (2019). Hypoxia Restrains Lipid Utilization via Protein Kinase A and Adipose Triglyceride Lipase Downregulation through Hypoxia-Inducible Factor. Molecular and cellular biology: 39 (2): e00390-18.
[3]	A. Holley, J. Pitman, J. Miller, S. Harding, P. Larsen, (2017). Glutathione peroxidase activity and expression levels are significantly increased in acute coronary syndromes. Journal of Investigative Medicine. 65 (5): 919-25.
[4]	L. Banjac, G. Banjac, J. Kotur-Stevuljević, V. Spasojević-Kalimanovska, T. Gojković, N. Bogavac-Stanojević, et al. (2018). Pro-Oxidants and Antioxidants in Retinopathy of Prematurity. Acta clinica Croatica: 57 (3): 458-63.
[5]	J. Momen-Beitollahi, A. Mansourian, F. Momen-Heravi, M. Amanlou, S. Obradov, M. Sahebjamee, (2010). Assessment of salivary and serum antioxidant status in patients with recurrent aphthous stomatitis. Med Oral patol Oral cir bucal. 15 (4): e557-61.
[6]	A. J. Bott, S. Maimouni, W-X. Zong, (2019). The Pleiotropic Effects of Glutamine Metabolism in Cancer. Cancers. 11 (6): 770.
[7]	J. Yang. (2018). The role of reactive oxygen species in angiogenesis and preventing tissue injury after brain ischemia. Microvascular research: 123; 62–67.
[8]	R. Alili, V. Nivet-Antoine, A. Saldmann, J-L. Golmard, C-H. Cottart, C. Laguillier, et al. (2018). Human catalase gene promoter haplotype and cardiometabolic improvement after bariatric surgery. Gene: 656: 17-21.
[9]	T. Nauser, J. M. Gebicki. (2019). Fast reaction of carbon free radicals with flavonoids and other aromatic compounds. Archives of biochemistry and biophysics: 108-107.
[10]	Y. Chen, S. Zheng, Z. Ju, C. Zhang, G. Tang, J. Wang, et al. (2018). Contribution of peroxisomal docking machinery to mycotoxin biosynthesis, pathogenicity and pexophagy in the plant pathogenic fungus Fusarium graminearum. Environmental microbiology. 20 (9): 3224-45.
[11]	A. H. Huang, (2012). Plant peroxisomes: Elsevier; pp 112-115.
[12]	A-K. Mix, U. Cenci, T. Heimerl, P. Marter, M-L. Wirkner, D. Moog. (2018). Identification and Localization of Peroxisomal Biogenesis Proteins Indicates the Presence of Peroxisomes in the Cryptophyte Guillardia theta and Other “Chromalveolates”. Genome biology and evolution. 10 (10): 2834-52.
[13]	C-C. Huang, R. S. Aronstam, D-R. Chen, Y-W. Huang. (2010). Oxidative stress, calcium homeostasis, and altered gene expression in human lung epithelial cells exposed to ZnO nanoparticles. Toxicology in vitro. 24 (1): 45-55.
[14]	T. J. Goodwin, J. M. Jessup, D. A. Wolf. (1992). Morphologic differentiation of colon carcinoma cell lines HT-29 and HT-29KM in rotating-wall vessels. In Vitro Cellular & Developmental Biology-Animal. 28 (1): 47-60.
[15]	K. Ferro, D. Ferro, F. Corrà, R. Bakiu, G. Santovito, J. Kurtz J, (2017). Cu, Zn superoxide dismutase genes in Tribolium castaneum: evolution, molecular characterisation, and gene expression during immune priming. Frontiers in immunology. 8: 1811.
[16]	B. Yadav, R. Srivastava, C. Dwivedi, P. Pramanik. (2008). Moisture sensor based on ZnO nanomaterial synthesized through oxalate route. Sensors and Actuators B: Chemical. 131 (1): 216-22.
[17]	A. Albanese, C. W. Chan. (2011). Effect of gold nanoparticle aggregation on cell uptake and toxicity. ACS nano. 5 (7): 5478-89.
[18]	K. Banerjee, V. R. Rai, M. Umashankar. (2019). Effect of peptide-conjugated nanoparticles on cell lines. Progress in biomaterials. 8 (1): 11-21.
[19]	X-Q. Liu, R-Z. Tang. (2017). Biological responses to nanomaterials: understanding nano-bio effects on cell behaviors. Drug delivery. 24 (2): 1-15.
[20]	W. Liu, Y. Wu, C. Wang, H. C. Li, T. Wang, C. Y. Liao, et al. (2010). Impact of silver nanoparticles on human cells: effect of particle size. Nanotoxicology. 4 (3): 319-30.
[21]	Js. Mosquera, M. Henriksen-Lacey, I. García, M. Martínez-Calvo, Js. Rodríguez, J. L. Mascareñas, et al. (2018). Cellular uptake of gold nanoparticles triggered by host–guest interactions. Journal of the American Chemical Society. 140 (13): 4469-72.
[22]	K. Kang, H. Jung, J-S. Lim. (2012). Cell death by polyvinylpyrrolidine-coated silver nanoparticles is mediated by ROS-dependent signaling. Biomolecules & therapeutics. 20 (4): 399.
[23]	R. Foldbjerg, D. A. Dang, H. Autrup. (2011). Cytotoxicity and genotoxicity of silver nanoparticles in the human lung cancer cell line, A549. Archives of toxicology. 85 (7): 743-50.
[24]	R. Foldbjerg, P. Olesen, M. Hougaard, D. A. Dang, H. J. Hoffmann, H. Autrup. (2009). PVP-coated silver nanoparticles and silver ions induce reactive oxygen species, apoptosis and necrosis in THP-1 monocytes. Toxicology letters. 190 (2): 156-62.

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APA Style

Metin Budak. (2019). Nanoparticles Induce Oxidative Stress in HT-29 Colon Adenocarcinoma Cell Line After 24 and 48 Hour Exposure. International Journal of Genetics and Genomics, 7(4), 110-114. https://doi.org/10.11648/j.ijgg.20190704.14

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ACS Style

Metin Budak. Nanoparticles Induce Oxidative Stress in HT-29 Colon Adenocarcinoma Cell Line After 24 and 48 Hour Exposure. Int. J. Genet. Genomics 2019, 7(4), 110-114. doi: 10.11648/j.ijgg.20190704.14

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AMA Style

Metin Budak. Nanoparticles Induce Oxidative Stress in HT-29 Colon Adenocarcinoma Cell Line After 24 and 48 Hour Exposure. Int J Genet Genomics. 2019;7(4):110-114. doi: 10.11648/j.ijgg.20190704.14

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@article{10.11648/j.ijgg.20190704.14,
  author = {Metin Budak},
  title = {Nanoparticles Induce Oxidative Stress in HT-29 Colon Adenocarcinoma Cell Line After 24 and 48 Hour Exposure},
  journal = {International Journal of Genetics and Genomics},
  volume = {7},
  number = {4},
  pages = {110-114},
  doi = {10.11648/j.ijgg.20190704.14},
  url = {https://doi.org/10.11648/j.ijgg.20190704.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijgg.20190704.14},
  abstract = {Nanoparticle research is currently an area of intense scientific research, due to a wide variety of potential applications in biomedical, optical, and electronic fields. Nanoparticles are of great scientific interest as they are effectively a bridge between bulk materials and atomic or molecular structures. Superoxide dismutase (SOD), Glutamine synthetase (GS), Catalase (CAT) are some of the defense mechanisms against cellular oxidative stress, especially against free oxygen radicals. In this study, we aimed to investigate that Ag, SiO2 and ZnO nanoparticles affect cancer cell lines (HT-29) and relationship between SOD, GS and CAT. We investigated that alterations in gene expressions of SOD, GS and CAT caused by exposure to nanoparticles in HT-29 cells. The difference between the Ct values (ΔCt) of the gene of interest was calculated for each experimental sample. As a result of Ag, SiO2 and ZnO nanoparticle application, there was a 2-fold increase in SOD and CAT expression in the first 24 hours compared to control. As a result of 48 hours of application, it was observed that Ag nanoparticles caused 4-fold increase in SOD and 6-fold statistically significant increase in CAT and GS expression of SiO2 nanoparticles. Consequently, after 48 hours of nanoparticle application, SiO2, CAT and GS expression were more effective than 24-hour application. Our results suggest that nanoparticles may cause increased oxidative stress in colon cells and may have therapeutic properties by affecting cancer cells in these aspects.},
 year = {2019}
}

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TY  - JOUR
T1  - Nanoparticles Induce Oxidative Stress in HT-29 Colon Adenocarcinoma Cell Line After 24 and 48 Hour Exposure
AU  - Metin Budak
Y1  - 2019/10/26
PY  - 2019
N1  - https://doi.org/10.11648/j.ijgg.20190704.14
DO  - 10.11648/j.ijgg.20190704.14
T2  - International Journal of Genetics and Genomics
JF  - International Journal of Genetics and Genomics
JO  - International Journal of Genetics and Genomics
SP  - 110
EP  - 114
PB  - Science Publishing Group
SN  - 2376-7359
UR  - https://doi.org/10.11648/j.ijgg.20190704.14
AB  - Nanoparticle research is currently an area of intense scientific research, due to a wide variety of potential applications in biomedical, optical, and electronic fields. Nanoparticles are of great scientific interest as they are effectively a bridge between bulk materials and atomic or molecular structures. Superoxide dismutase (SOD), Glutamine synthetase (GS), Catalase (CAT) are some of the defense mechanisms against cellular oxidative stress, especially against free oxygen radicals. In this study, we aimed to investigate that Ag, SiO2 and ZnO nanoparticles affect cancer cell lines (HT-29) and relationship between SOD, GS and CAT. We investigated that alterations in gene expressions of SOD, GS and CAT caused by exposure to nanoparticles in HT-29 cells. The difference between the Ct values (ΔCt) of the gene of interest was calculated for each experimental sample. As a result of Ag, SiO2 and ZnO nanoparticle application, there was a 2-fold increase in SOD and CAT expression in the first 24 hours compared to control. As a result of 48 hours of application, it was observed that Ag nanoparticles caused 4-fold increase in SOD and 6-fold statistically significant increase in CAT and GS expression of SiO2 nanoparticles. Consequently, after 48 hours of nanoparticle application, SiO2, CAT and GS expression were more effective than 24-hour application. Our results suggest that nanoparticles may cause increased oxidative stress in colon cells and may have therapeutic properties by affecting cancer cells in these aspects.
VL  - 7
IS  - 4
ER  -

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Author Information

Metin Budak

Department of Biophysics, Trakya University, Edirne, Turkey

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Plain Text BibTeX RIS

APA Style

Metin Budak. (2019). Nanoparticles Induce Oxidative Stress in HT-29 Colon Adenocarcinoma Cell Line After 24 and 48 Hour Exposure. International Journal of Genetics and Genomics, 7(4), 110-114. https://doi.org/10.11648/j.ijgg.20190704.14

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ACS Style

Metin Budak. Nanoparticles Induce Oxidative Stress in HT-29 Colon Adenocarcinoma Cell Line After 24 and 48 Hour Exposure. Int. J. Genet. Genomics 2019, 7(4), 110-114. doi: 10.11648/j.ijgg.20190704.14

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AMA Style

Metin Budak. Nanoparticles Induce Oxidative Stress in HT-29 Colon Adenocarcinoma Cell Line After 24 and 48 Hour Exposure. Int J Genet Genomics. 2019;7(4):110-114. doi: 10.11648/j.ijgg.20190704.14

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@article{10.11648/j.ijgg.20190704.14,
  author = {Metin Budak},
  title = {Nanoparticles Induce Oxidative Stress in HT-29 Colon Adenocarcinoma Cell Line After 24 and 48 Hour Exposure},
  journal = {International Journal of Genetics and Genomics},
  volume = {7},
  number = {4},
  pages = {110-114},
  doi = {10.11648/j.ijgg.20190704.14},
  url = {https://doi.org/10.11648/j.ijgg.20190704.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijgg.20190704.14},
  abstract = {Nanoparticle research is currently an area of intense scientific research, due to a wide variety of potential applications in biomedical, optical, and electronic fields. Nanoparticles are of great scientific interest as they are effectively a bridge between bulk materials and atomic or molecular structures. Superoxide dismutase (SOD), Glutamine synthetase (GS), Catalase (CAT) are some of the defense mechanisms against cellular oxidative stress, especially against free oxygen radicals. In this study, we aimed to investigate that Ag, SiO2 and ZnO nanoparticles affect cancer cell lines (HT-29) and relationship between SOD, GS and CAT. We investigated that alterations in gene expressions of SOD, GS and CAT caused by exposure to nanoparticles in HT-29 cells. The difference between the Ct values (ΔCt) of the gene of interest was calculated for each experimental sample. As a result of Ag, SiO2 and ZnO nanoparticle application, there was a 2-fold increase in SOD and CAT expression in the first 24 hours compared to control. As a result of 48 hours of application, it was observed that Ag nanoparticles caused 4-fold increase in SOD and 6-fold statistically significant increase in CAT and GS expression of SiO2 nanoparticles. Consequently, after 48 hours of nanoparticle application, SiO2, CAT and GS expression were more effective than 24-hour application. Our results suggest that nanoparticles may cause increased oxidative stress in colon cells and may have therapeutic properties by affecting cancer cells in these aspects.},
 year = {2019}
}

Copy | Download

TY  - JOUR
T1  - Nanoparticles Induce Oxidative Stress in HT-29 Colon Adenocarcinoma Cell Line After 24 and 48 Hour Exposure
AU  - Metin Budak
Y1  - 2019/10/26
PY  - 2019
N1  - https://doi.org/10.11648/j.ijgg.20190704.14
DO  - 10.11648/j.ijgg.20190704.14
T2  - International Journal of Genetics and Genomics
JF  - International Journal of Genetics and Genomics
JO  - International Journal of Genetics and Genomics
SP  - 110
EP  - 114
PB  - Science Publishing Group
SN  - 2376-7359
UR  - https://doi.org/10.11648/j.ijgg.20190704.14
AB  - Nanoparticle research is currently an area of intense scientific research, due to a wide variety of potential applications in biomedical, optical, and electronic fields. Nanoparticles are of great scientific interest as they are effectively a bridge between bulk materials and atomic or molecular structures. Superoxide dismutase (SOD), Glutamine synthetase (GS), Catalase (CAT) are some of the defense mechanisms against cellular oxidative stress, especially against free oxygen radicals. In this study, we aimed to investigate that Ag, SiO2 and ZnO nanoparticles affect cancer cell lines (HT-29) and relationship between SOD, GS and CAT. We investigated that alterations in gene expressions of SOD, GS and CAT caused by exposure to nanoparticles in HT-29 cells. The difference between the Ct values (ΔCt) of the gene of interest was calculated for each experimental sample. As a result of Ag, SiO2 and ZnO nanoparticle application, there was a 2-fold increase in SOD and CAT expression in the first 24 hours compared to control. As a result of 48 hours of application, it was observed that Ag nanoparticles caused 4-fold increase in SOD and 6-fold statistically significant increase in CAT and GS expression of SiO2 nanoparticles. Consequently, after 48 hours of nanoparticle application, SiO2, CAT and GS expression were more effective than 24-hour application. Our results suggest that nanoparticles may cause increased oxidative stress in colon cells and may have therapeutic properties by affecting cancer cells in these aspects.
VL  - 7
IS  - 4
ER  -

Copy | Download