Comparative Study of Antibacterial Activity of Vancomycin and Chemically Treated Chitosan Prepared from Shrimp (Macrobrachium Rosenbergii) Waste

Rashna Gulshan; Md. Tanvir Sarwar; Md. Abdullah Al Mamun; Md. Jahangir Alam; Sabir Hossain; M. Masihul Alam

doi:doi:10.11648/j.ijnfs.20130206.17

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Comparative Study of Antibacterial Activity of Vancomycin and Chemically Treated Chitosan Prepared from Shrimp (Macrobrachium Rosenbergii) Waste

Rashna Gulshan, Md. Tanvir Sarwar, Md. Abdullah Al Mamun, Md. Jahangir Alam, Sabir Hossain, M. Masihul Alam

Published in International Journal of Nutrition and Food Sciences (Volume 2, Issue 6)

Received: 15 August 2013 Published: 20 November 2013

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Abstract

Chitosan is derived from exoskeleton of crustaceans (such as crabs and shrimp) and cell walls of fungi has a number of commercial and possible biomedical uses is produced commercially by deacetylation of chitin is a linear polysaccharide composed of randomly distributed β-(1-4)-linked D-glucosamine (deacetylated unit) and N-acetyl-D-glucosamine (acetylated unit). The structural element This study was conducted to compare the antibacterial activity of chitosan with the combination of chitosan and vancomycin against gram negative Escherichia coli and gram positive Staphylococcus aureus. Optical density (OD) value measurement technique was undertaken to measure antibacterial activity. After incubation, turbidimetric measurements of bacterial growth of sets of test tubes were taken after every 4 hours, for 48 hours and then compared. For S. aureus and chitosan the average absorbance were found as 0.448, 0.457, 0.492, 0.532, 0.582, 0.645, 0.683, 0.724, 0.751, 0.793, 0.840, and 0.896, while for S. aureus with both chitosan and vancomycin, absorbance were 0.267, 0.286, 0.321, 0.346, 0.386, 0.431, 0.467, 0.475, 0.509, 0.541, 0.565, and 0.629. Again for E. coli and chitosan the average absorbance were found as 0.407, 0.429, 0.464, 0.506, 0.564, 0.6, 0.645, 0.703, 0.756, 0.815, 0.850, and 0.901, while E. coli with both chitosan and vancomycin, absorbance were 0.213, 0.233, 0.322, 0.347, 0.409, 0.446, 0.475, 0.511, 0.545, 0.582, 0.628, and 0.647. Both of these revealed that chitosan and vancomycin together possess higher antibacterial activity against gram positive and gram-negative bacteria than chitosan solitarily used. Statistical analysis of the both study also represented in Mean±3SD. Comparison of the antibacterial activity against E. coli and S. aureus revealed that chitosan and vancomycin posses similar antibacterial activity against gram positive and gram negative bacteria.

Published in	International Journal of Nutrition and Food Sciences (Volume 2, Issue 6)
DOI	10.11648/j.ijnfs.20130206.17
Page(s)	307-311
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2013. Published by Science Publishing Group

Keywords

Antibacterial activity, Chitosan, Vancomycin

References

[1]	Jayakumar, R.; New, N.T.; Tokura, S.; Tamura, H. Sulfated Chitin and Chitosan as novel biomaterial. Int. J. Biol. Macromol., 40, 175-181, 2007.
[2]	Austin P.R., Brine C.J., Castle J.E. ans Zikakis J.P. Chitin: New facets of research. Science, 749, 1981.
[3]	Matthew B. Avisona, Peter M. Bennettb, Robin A. Howec and Timothy R. Walsh. Preliminary analysis of the genetic basis for vancomycin resistance in Staphylococcus aureusstrain Mu50. J. Antimicrob. Chemother. 49 (2), 255-260, 2002.
[4]	Abu Tareq, M. Masihul Alam, Md. Salim Raza, Md. Tanvir Sarwar, Z. Fardous, Chowdhury, M. Sabir Hossain. Comparative study of antibacterial activity of chitin and chemically treated chitosan prepared from shrimp (Macrobrachium rosenbergii) shell waste. Int. Res. J. of Microbiol. (IRJM) 2013 (2013)
[5]	Koide S.S. Chitin-Chitosan: properties, Benefits and Risks. Nutrition Research. 18(6), 1091-1101, 1998.
[6]	Rout S. K. Physicochemical, Functional, and Spectroscopic analysis of crawfish chitin and chitosan as affected by process modification. Dissertation, 2001.
[7]	Qin CQ, Li HR, Xiao Q, Liu Y, Zhu JC, Du YM. Water-solubility of chitosan and its antimicrobial activity. Carbohydrate Polymers. 63, 367-374, 2006.
[8]	Chen Y M, Chung YC, Wang LW, Chen KT, Li SY. Antibacterial activity of chitosan- based matrixes on oral pathogens. Health A Tox Hazard Subst Environ Eng. J. Environ Sci. 37, 1379-1390, 2002.
[9]	Cuero, R.G. Antimicrobial action of exogenous chitosan. EXS. 1999. 87.p.315-333. Del Blanco L.F., Rodriguez M.S., Schulz P.C., Agullo E. Influence of the deacetylation degree on chitosan emulsification properties. Colloid Polymer Science. 277, 1087-1092. 1999.
[10]	Abu Tareq, M. Masihul Alam, Md. Salim Raza, Md. Tanvir Sarwar, Z. Fardous, Alamgir Z. Chowdhury, Sabir Hossain. Comparative study of antibacterial activity of chitin and chemically treated chitosan prepared from shrimp (Macrobrachium rosenbergii) shell waste International Journal of Virology Microbiology (JVM)Vol. 2013. 1-9, 2013.
[11]	Weiner M. L. An overview of the regulatory status and of the safety of chitin and chitosan as food and pharmaceutical ingredients. In: Brine, C.J., Sandford, P.A., Zikakis, J.P. (Eds.).1992.
[12]	No, H. K. & Meyers, S. P. "Preparation and Characterization of Chitin and Chitosan-A Review," Journal of Aquatic Food Product Technology. 4(2). 27-52. 1995
[13]	Zizewitz, Paul W., New York, N. Y. Glencoe/ M.CGraw-Hill,Glencoe Physics. ISBN 0028254732. P. 395. 1999.
[14]	Bosso JA. The antimicrobial armamentarium: evaluating current and future treatment options. Pharmacotherapy. 25,55S-62S, 2005.
[15]	Ling TKW, Xiong J, Yu Y. et al. Multicenter antimicrobial susceptibility survey of Gram-negative bacteria isolated from patients with community-acquired infections in the People's Republic of China. Antimicrob Agents Chemother. 50, 374-378, 2006.
[16]	George ME, Robert C, Moellering JR. Antimicrobial Combinations. Antibiotics in Laboratory Medicine 4th ed. Baltimore: The Williams & Wilkins Co. 1996.

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Rashna Gulshan, Md. Tanvir Sarwar, Md. Abdullah Al Mamun, Md. Jahangir Alam, Sabir Hossain, et al. (2013). Comparative Study of Antibacterial Activity of Vancomycin and Chemically Treated Chitosan Prepared from Shrimp (Macrobrachium Rosenbergii) Waste. International Journal of Nutrition and Food Sciences, 2(6), 307-311. https://doi.org/10.11648/j.ijnfs.20130206.17

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Rashna Gulshan; Md. Tanvir Sarwar; Md. Abdullah Al Mamun; Md. Jahangir Alam; Sabir Hossain, et al. Comparative Study of Antibacterial Activity of Vancomycin and Chemically Treated Chitosan Prepared from Shrimp (Macrobrachium Rosenbergii) Waste. Int. J. Nutr. Food Sci. 2013, 2(6), 307-311. doi: 10.11648/j.ijnfs.20130206.17

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Rashna Gulshan, Md. Tanvir Sarwar, Md. Abdullah Al Mamun, Md. Jahangir Alam, Sabir Hossain, et al. Comparative Study of Antibacterial Activity of Vancomycin and Chemically Treated Chitosan Prepared from Shrimp (Macrobrachium Rosenbergii) Waste. Int J Nutr Food Sci. 2013;2(6):307-311. doi: 10.11648/j.ijnfs.20130206.17

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@article{10.11648/j.ijnfs.20130206.17,
  author = {Rashna Gulshan and Md. Tanvir Sarwar and Md. Abdullah Al Mamun and Md. Jahangir Alam and Sabir Hossain and M. Masihul Alam},
  title = {Comparative Study of Antibacterial Activity of Vancomycin and Chemically Treated Chitosan Prepared from Shrimp (Macrobrachium Rosenbergii) Waste},
  journal = {International Journal of Nutrition and Food Sciences},
  volume = {2},
  number = {6},
  pages = {307-311},
  doi = {10.11648/j.ijnfs.20130206.17},
  url = {https://doi.org/10.11648/j.ijnfs.20130206.17},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijnfs.20130206.17},
  abstract = {Chitosan is derived from exoskeleton of crustaceans (such as crabs and shrimp) and cell walls of fungi has a number of commercial and possible biomedical uses is produced commercially by deacetylation of chitin is a linear polysaccharide composed of randomly distributed β-(1-4)-linked D-glucosamine (deacetylated unit) and N-acetyl-D-glucosamine (acetylated unit). The structural element This study was conducted to compare the antibacterial activity of chitosan with the combination of chitosan and vancomycin against gram negative Escherichia coli and gram positive Staphylococcus aureus.  Optical density (OD) value measurement technique was undertaken to measure antibacterial activity. After incubation, turbidimetric measurements of bacterial growth of sets of test tubes were taken after every 4 hours, for 48 hours and then compared. For S. aureus and chitosan the  average absorbance were found as 0.448, 0.457, 0.492, 0.532, 0.582, 0.645, 0.683, 0.724, 0.751, 0.793, 0.840, and 0.896, while for S. aureus with both chitosan and vancomycin, absorbance were 0.267, 0.286, 0.321, 0.346, 0.386, 0.431, 0.467, 0.475, 0.509, 0.541, 0.565, and 0.629. Again for E. coli and chitosan the  average absorbance were found as 0.407, 0.429, 0.464, 0.506, 0.564, 0.6, 0.645, 0.703, 0.756, 0.815, 0.850, and 0.901, while E. coli with both chitosan and vancomycin, absorbance were 0.213, 0.233, 0.322, 0.347, 0.409, 0.446, 0.475, 0.511, 0.545, 0.582, 0.628, and 0.647. Both of these revealed that chitosan and vancomycin together possess higher antibacterial activity against gram positive and gram-negative bacteria than chitosan solitarily used. Statistical analysis of the both study also represented in Mean±3SD. Comparison of the antibacterial activity against E. coli and S. aureus revealed that chitosan and vancomycin posses similar antibacterial activity against gram positive and gram negative bacteria.},
 year = {2013}
}

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TY - JOUR
T1 - Comparative Study of Antibacterial Activity of Vancomycin and Chemically Treated Chitosan Prepared from Shrimp (Macrobrachium Rosenbergii) Waste
AU - Rashna Gulshan
AU - Md. Tanvir Sarwar
AU - Md. Abdullah Al Mamun
AU - Md. Jahangir Alam
AU - Sabir Hossain
AU - M. Masihul Alam
Y1 - 2013/11/20
PY - 2013
N1 - https://doi.org/10.11648/j.ijnfs.20130206.17
DO - 10.11648/j.ijnfs.20130206.17
T2 - International Journal of Nutrition and Food Sciences
JF - International Journal of Nutrition and Food Sciences
JO - International Journal of Nutrition and Food Sciences
SP - 307
EP - 311
PB - Science Publishing Group
SN - 2327-2716
UR - https://doi.org/10.11648/j.ijnfs.20130206.17
AB - Chitosan is derived from exoskeleton of crustaceans (such as crabs and shrimp) and cell walls of fungi has a number of commercial and possible biomedical uses is produced commercially by deacetylation of chitin is a linear polysaccharide composed of randomly distributed β-(1-4)-linked D-glucosamine (deacetylated unit) and N-acetyl-D-glucosamine (acetylated unit). The structural element This study was conducted to compare the antibacterial activity of chitosan with the combination of chitosan and vancomycin against gram negative Escherichia coli and gram positive Staphylococcus aureus. Optical density (OD) value measurement technique was undertaken to measure antibacterial activity. After incubation, turbidimetric measurements of bacterial growth of sets of test tubes were taken after every 4 hours, for 48 hours and then compared. For S. aureus and chitosan the average absorbance were found as 0.448, 0.457, 0.492, 0.532, 0.582, 0.645, 0.683, 0.724, 0.751, 0.793, 0.840, and 0.896, while for S. aureus with both chitosan and vancomycin, absorbance were 0.267, 0.286, 0.321, 0.346, 0.386, 0.431, 0.467, 0.475, 0.509, 0.541, 0.565, and 0.629. Again for E. coli and chitosan the average absorbance were found as 0.407, 0.429, 0.464, 0.506, 0.564, 0.6, 0.645, 0.703, 0.756, 0.815, 0.850, and 0.901, while E. coli with both chitosan and vancomycin, absorbance were 0.213, 0.233, 0.322, 0.347, 0.409, 0.446, 0.475, 0.511, 0.545, 0.582, 0.628, and 0.647. Both of these revealed that chitosan and vancomycin together possess higher antibacterial activity against gram positive and gram-negative bacteria than chitosan solitarily used. Statistical analysis of the both study also represented in Mean±3SD. Comparison of the antibacterial activity against E. coli and S. aureus revealed that chitosan and vancomycin posses similar antibacterial activity against gram positive and gram negative bacteria.
VL - 2
IS - 6
ER -

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Author Information

Rashna Gulshan

Dept. of Applied Nutrition and Food Technology, Islamic University, Kushtia-7003, Bangladesh
Md. Tanvir Sarwar

Dept. of Applied Nutrition and Food Technology, Islamic University, Kushtia-7003, Bangladesh
Md. Abdullah Al Mamun

Dept of Genetic Engineering and Biotechnology, Shahjalal University of Science & Technology, Sylhet-3114, Bangladesh
Md. Jahangir Alam

Dept of Genetic Engineering and Biotechnology, Shahjalal University of Science & Technology, Sylhet-3114, Bangladesh
Sabir Hossain

Biochemistry and Molecular Biology Division, Jahangirnagar University, Savar, Dhaka, Bangladesh
M. Masihul Alam

Dept. of Applied Nutrition and Food Technology, Islamic University, Kushtia-7003, Bangladesh

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APA Style

Rashna Gulshan, Md. Tanvir Sarwar, Md. Abdullah Al Mamun, Md. Jahangir Alam, Sabir Hossain, et al. (2013). Comparative Study of Antibacterial Activity of Vancomycin and Chemically Treated Chitosan Prepared from Shrimp (Macrobrachium Rosenbergii) Waste. International Journal of Nutrition and Food Sciences, 2(6), 307-311. https://doi.org/10.11648/j.ijnfs.20130206.17

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Rashna Gulshan; Md. Tanvir Sarwar; Md. Abdullah Al Mamun; Md. Jahangir Alam; Sabir Hossain, et al. Comparative Study of Antibacterial Activity of Vancomycin and Chemically Treated Chitosan Prepared from Shrimp (Macrobrachium Rosenbergii) Waste. Int. J. Nutr. Food Sci. 2013, 2(6), 307-311. doi: 10.11648/j.ijnfs.20130206.17

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AMA Style

Rashna Gulshan, Md. Tanvir Sarwar, Md. Abdullah Al Mamun, Md. Jahangir Alam, Sabir Hossain, et al. Comparative Study of Antibacterial Activity of Vancomycin and Chemically Treated Chitosan Prepared from Shrimp (Macrobrachium Rosenbergii) Waste. Int J Nutr Food Sci. 2013;2(6):307-311. doi: 10.11648/j.ijnfs.20130206.17

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@article{10.11648/j.ijnfs.20130206.17,
  author = {Rashna Gulshan and Md. Tanvir Sarwar and Md. Abdullah Al Mamun and Md. Jahangir Alam and Sabir Hossain and M. Masihul Alam},
  title = {Comparative Study of Antibacterial Activity of Vancomycin and Chemically Treated Chitosan Prepared from Shrimp (Macrobrachium Rosenbergii) Waste},
  journal = {International Journal of Nutrition and Food Sciences},
  volume = {2},
  number = {6},
  pages = {307-311},
  doi = {10.11648/j.ijnfs.20130206.17},
  url = {https://doi.org/10.11648/j.ijnfs.20130206.17},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijnfs.20130206.17},
  abstract = {Chitosan is derived from exoskeleton of crustaceans (such as crabs and shrimp) and cell walls of fungi has a number of commercial and possible biomedical uses is produced commercially by deacetylation of chitin is a linear polysaccharide composed of randomly distributed β-(1-4)-linked D-glucosamine (deacetylated unit) and N-acetyl-D-glucosamine (acetylated unit). The structural element This study was conducted to compare the antibacterial activity of chitosan with the combination of chitosan and vancomycin against gram negative Escherichia coli and gram positive Staphylococcus aureus.  Optical density (OD) value measurement technique was undertaken to measure antibacterial activity. After incubation, turbidimetric measurements of bacterial growth of sets of test tubes were taken after every 4 hours, for 48 hours and then compared. For S. aureus and chitosan the  average absorbance were found as 0.448, 0.457, 0.492, 0.532, 0.582, 0.645, 0.683, 0.724, 0.751, 0.793, 0.840, and 0.896, while for S. aureus with both chitosan and vancomycin, absorbance were 0.267, 0.286, 0.321, 0.346, 0.386, 0.431, 0.467, 0.475, 0.509, 0.541, 0.565, and 0.629. Again for E. coli and chitosan the  average absorbance were found as 0.407, 0.429, 0.464, 0.506, 0.564, 0.6, 0.645, 0.703, 0.756, 0.815, 0.850, and 0.901, while E. coli with both chitosan and vancomycin, absorbance were 0.213, 0.233, 0.322, 0.347, 0.409, 0.446, 0.475, 0.511, 0.545, 0.582, 0.628, and 0.647. Both of these revealed that chitosan and vancomycin together possess higher antibacterial activity against gram positive and gram-negative bacteria than chitosan solitarily used. Statistical analysis of the both study also represented in Mean±3SD. Comparison of the antibacterial activity against E. coli and S. aureus revealed that chitosan and vancomycin posses similar antibacterial activity against gram positive and gram negative bacteria.},
 year = {2013}
}

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