E. coli O157 is responsible for a number of human infections through the consumption of foods of animal origin, particularly those originating from cattle. Food poisoning by Escherichia coli O157 causes diarrhea, bloody stools and abdominal pain in humans, and may lead to severe kidney failure or brain injury. For these reasons, E. coli O157 has been the subject of increasingly stringent international regulations. A novel peptide nucleic acid (PNA) array was developed to identify E. coli O157 quickly and accurately. This PNA array exhibited high specificity, significantly detecting E. coli O157 strains without cross-reacting with other bacterial strains. The detection limit of the E. coli O157 PNA array was 100 CFU/ml. The E. coli O157 PNA array is indeed a better option for early detection and accurate diagnosis of the causative agent of a food-borne disease outbreak, which is crucial in planning and implementing strategic measures to prevent and control widespread outbreaks.
| Published in | International Journal of Nutrition and Food Sciences (Volume 3, Issue 2) |
| DOI | 10.11648/j.ijnfs.20140302.11 |
| Page(s) | 14-18 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2014. Published by Science Publishing Group |
Escherichia coli O157, Peptide Nucleic Acid Array, Food Poisoning
| [1] | Beau-Faller, M., Legrain, M., Voegeli, A.C., Guerin, E., Lavaux, T., Ruppert, A.M., Neuvile, A., Massard, G., Wihlm, J.M., Quoix, E., Oudet, P., Gaub, M.P., 2009. Detection of K-Ras mutations in tumour samples of patients with non-small cell lung cancer using PNA-mediated PCR clamping. Br. J. Cancer. 100, 985–992. |
| [2] | Brandt, O., Feldner, J., Stephan, A., Schroder, M., Schnolzer, M., Arlinghaus, H.F., Hoheisel, J.D., Jacob, A., 2003. PNA microarrays for hybridization of unlabelled DNA samples. Nucleic. Acids. Res. 31, e119. |
| [3] | Brandt, O., Hoheisel, J.D., 2004. Peptide nucleic acids on microarrays and other biosensors. Trends. Biotechnol. 22, 617-622. |
| [4] | Choi, J.J., Kim, C., Park, H., 2009. Peptide nucleic acid-based array for detecting and genotyping human papillomaviruses. J. Clin. Microbiol. 47, 1785-1790. |
| [5] | Gannon, V.P.J., D'souza, S., Graham, T., King, R.K., Rahn, K., Read, S., 1997. Use of the flagellar H7 gene as a target in multiplex PCR assays and improved specificity in identification of enterohemorrhagic Esherichia coli strains. J. Clin. Microbiol. 35, 656-662. |
| [6] | Jo, M.Y., Kim, J.H., Lim, J.H., Kang, M.Y., Koh, H.B., Park, Y.H., Yoon, D.Y., Chae, J.S., Eo, S.K., Lee, J.H., 2004. Prevalence and characteristics of Escherichia coli O157 from major food animals in Korea. Int. J. Food. Microbiol. 95, 41-49. |
| [7] | Jung, B.Y., Jung, S.C., Cho, D.H., Kim, J.Y., Park, Y.H., Shin, S.J., Kim, S.S., Kim, B.H., 1998. Development of an enzyme immunoassay for detection of Escherichia coli O157 in meat. Korean. J. Vet. Res. 38, 745-750. |
| [8] | Karmali, M.A., 1989. Infection by verocytotoxin-producing Escherichia coli. Clin. Microbiol. Rev. 2, 15-38. |
| [9] | Kempf, V.A., Trebesius, K., Autenrieth, I.B., 2000. Fluorescent in situ hybridization allows rapid identification of microorganisms in blood cultures. J. Clin. Microbiol. 38, 830-838. |
| [10] | Kim, J.Y., Kim, S.H., Kwon, N.H., Bae, W.K., Lim, J.Y., Koo, H.C., Kim, J.M., Noh, K.M., Jung, W. K., Park, K.T., Park ,Y.H., 2005. Isolation and identification of Escherchia coli O157:H7 using different detection methods and molecular determination by multiplex PCR and RAPD. J. Vet .Sci. 6, 7-19. |
| [11] | Komiyama, M., Ye, S., Liang, X., Yamamoto, Y., Tomita, T., Zhou, J.M., Aburatani, H., 2003. PNA for one-base differentiating protection of DNA from nuclease and its use for SNPs detection. J. Am. Chem. Soc. 125, 3758–3762. |
| [12] | Kwon, D., Kim, O., Chae, C., 1999. Prevalence of genotypes for firmbriae and enterotoxins and of O serogroups in Escherichia coli isolated from diarrheic piglets in Korea. J. Vet. Diagn. Invest. 11, 146-151. |
| [13] | Nielsen, P.E., Egholm, M., Berg, R.H., Buchardt, O., 1991. Sequence-selective recognition of DNA by stand displacement with a thymine-substituted polyamide. Science. 254, 1497-1500. |
| [14] | Nielsen, P.E., 1998. Structural and biological properties of peptide nucleic acid (PNA). Pure. Appl .Chem. 70, 105-110. |
| [15] | Pellestor, F., Paulasova, P., 2004. The peptide nucleic acids, efficient tools for molecular diagnosis (Review). Int. J. Mol. Med. 13, 521-525. |
| [16] | Quinones, B., Swimley, M.S., Taylor, A.W., Dawson, E.D., 2011. Identification of Escherchia coli O157 by using a novel colorimetric detection method with DNA microarrays. Foodborne. Pathog. Dis. 8, 705-711. |
| [17] | Rangel, J.M., Sparling, P.H., Crowe, C., Griffin, P.M., Swerdlow, D.L., 2005. Epidemiology of Escherichia coli O157:H7 outbreaks, United states, 1982-2002. Emerg. Infect. Dis. 11, 603-609. |
| [18] | Ratilainen, T., Holmén, A., Tuite, E., Nielsen, P.E., Norden, B., 2000. Thermodynamics of sequence‐specific binding of PNA to DNA. Biochemistry. 39, 7781–7791. |
| [19] | Riley, L.W., Remis, R.S., Helgerson, S.D., McGee, H.B., Wells, J.G., Davis, B.R., Hebert, R.J., Olcott, E.S., Johnson, L.M., Hargrett, N.T., Blake, P.A., Cohen, M.L., 1983. Hemorrhagic colitis associated with a rare Escherichia coli serotype. N. Engl. J. Med. 308, 681-685. |
| [20] | Sheng, H., Lim, J.Y., Watkins, M.K., Minnich, S.A., Hovde, C.J., 2008. Characterization of an Escherichia coli O157:H7 O-antigen deletion mutant and effect of the deletion on bacterial persistence in the mouse intestine and colonization at the bovine terminal rectal mucosa. Appl. Environ. Microbiol. 74, 5015–5022. |
| [21] | Stender, H., 2003. PNA FISH: an intelligent stain for rapid diagnosis of infectious diseases. Expert. Rev. Mol. Diagn. 3, 649-655. |
| [22] | Velusamy, V., Arshak, K., Korostynska, O., Oliwa, K., Adley, C., 2010. An overview of foodborne pathogen detection : In the perspective of biosensors. Biotechnol. Adv. 28, 232-254. |
| [23] | Wang, L., Reeves, P.R., 1998. Organization of Escherichia coli O157 O antigen gene cluster and identification of its specific genes. Infect. Immun. 66, 3545–3551. |
| [24] | Weiler, J., Gausepohl, H., Hauser, N., Jensen, O.N., Hoheisel, J.D., 1997. Hybridisation based DNA screening on peptide nucleic acid (PNA) oligomer arrays. Nucleic. Acids. Res. 25, 2792-2799. |
| [25] | Wells, J.S., Davis, B.R., Wachsmuth, I.K., Riley, L.W., Remis, R.S., Sokolow, R., Morris, G.K., 1983. Laboratory investigation of hemorrhagic colitis outbreaks associated with a rare Escherichia coli serotype. J. Clin. Microbiol. 18, 512-520. |
| [26] | Willshaw, G.A., Cheasty, T., Smith, H.R., O'Brien, S.J., Adak, G.K., 2001. Verocytotoxin-producing Escherichia coli (VTEC) O157 and other VTEC from human infections in England and Wales: 1995-1998. J. Med. Microbiol. 50, 135-142. |
| [27] | You, J.Y., Moon, B.M., Oh, I.G., Baek, B.K., Li, L.G., Kim, B.S., Stein, B.D., Lee, J.H., 2006. Antimicrobial resistance of Escherichia coli O157 from cattle in Korea. Int. J. Food. Microbiol. 106, 74-78. |
APA Style
Ki-Eun Lee, Woo-Seog Jeong, Seong-In Lim, Se-Ryun Kim, Yong-Kuk Kwon, et al. (2014). A Novel Detection Method for Escherichia Coli O157 by Peptide Nucleic Acid Array. International Journal of Nutrition and Food Sciences, 3(2), 14-18. https://doi.org/10.11648/j.ijnfs.20140302.11
ACS Style
Ki-Eun Lee; Woo-Seog Jeong; Seong-In Lim; Se-Ryun Kim; Yong-Kuk Kwon, et al. A Novel Detection Method for Escherichia Coli O157 by Peptide Nucleic Acid Array. Int. J. Nutr. Food Sci. 2014, 3(2), 14-18. doi: 10.11648/j.ijnfs.20140302.11
AMA Style
Ki-Eun Lee, Woo-Seog Jeong, Seong-In Lim, Se-Ryun Kim, Yong-Kuk Kwon, et al. A Novel Detection Method for Escherichia Coli O157 by Peptide Nucleic Acid Array. Int J Nutr Food Sci. 2014;3(2):14-18. doi: 10.11648/j.ijnfs.20140302.11
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Download@article{10.11648/j.ijnfs.20140302.11,
author = {Ki-Eun Lee and Woo-Seog Jeong and Seong-In Lim and Se-Ryun Kim and Yong-Kuk Kwon and Jin-San Moon and Soo-Yeon Cho and Jae-Young Song and Dong-Jun An},
title = {A Novel Detection Method for Escherichia Coli O157 by Peptide Nucleic Acid Array},
journal = {International Journal of Nutrition and Food Sciences},
volume = {3},
number = {2},
pages = {14-18},
doi = {10.11648/j.ijnfs.20140302.11},
url = {https://doi.org/10.11648/j.ijnfs.20140302.11},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijnfs.20140302.11},
abstract = {E. coli O157 is responsible for a number of human infections through the consumption of foods of animal origin, particularly those originating from cattle. Food poisoning by Escherichia coli O157 causes diarrhea, bloody stools and abdominal pain in humans, and may lead to severe kidney failure or brain injury. For these reasons, E. coli O157 has been the subject of increasingly stringent international regulations. A novel peptide nucleic acid (PNA) array was developed to identify E. coli O157 quickly and accurately. This PNA array exhibited high specificity, significantly detecting E. coli O157 strains without cross-reacting with other bacterial strains. The detection limit of the E. coli O157 PNA array was 100 CFU/ml. The E. coli O157 PNA array is indeed a better option for early detection and accurate diagnosis of the causative agent of a food-borne disease outbreak, which is crucial in planning and implementing strategic measures to prevent and control widespread outbreaks.},
year = {2014}
}
TY - JOUR T1 - A Novel Detection Method for Escherichia Coli O157 by Peptide Nucleic Acid Array AU - Ki-Eun Lee AU - Woo-Seog Jeong AU - Seong-In Lim AU - Se-Ryun Kim AU - Yong-Kuk Kwon AU - Jin-San Moon AU - Soo-Yeon Cho AU - Jae-Young Song AU - Dong-Jun An Y1 - 2014/01/20 PY - 2014 N1 - https://doi.org/10.11648/j.ijnfs.20140302.11 DO - 10.11648/j.ijnfs.20140302.11 T2 - International Journal of Nutrition and Food Sciences JF - International Journal of Nutrition and Food Sciences JO - International Journal of Nutrition and Food Sciences SP - 14 EP - 18 PB - Science Publishing Group SN - 2327-2716 UR - https://doi.org/10.11648/j.ijnfs.20140302.11 AB - E. coli O157 is responsible for a number of human infections through the consumption of foods of animal origin, particularly those originating from cattle. Food poisoning by Escherichia coli O157 causes diarrhea, bloody stools and abdominal pain in humans, and may lead to severe kidney failure or brain injury. For these reasons, E. coli O157 has been the subject of increasingly stringent international regulations. A novel peptide nucleic acid (PNA) array was developed to identify E. coli O157 quickly and accurately. This PNA array exhibited high specificity, significantly detecting E. coli O157 strains without cross-reacting with other bacterial strains. The detection limit of the E. coli O157 PNA array was 100 CFU/ml. The E. coli O157 PNA array is indeed a better option for early detection and accurate diagnosis of the causative agent of a food-borne disease outbreak, which is crucial in planning and implementing strategic measures to prevent and control widespread outbreaks. VL - 3 IS - 2 ER -
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